Monochromatic heterodyne fiber-optic profile sensor for spatially resolved velocity measurements with frequency division multiplexing

Investigating shear flows is important in technical applications as well as in fundamental research. Velocity measurements with high spatial resolution are necessary. Laser Doppler anemometry allows nonintrusive precise measurements, but the spatial resolution is limited by the size of the measurement volume to approximately 50 microm. A new laser Doppler profile sensor is proposed, enabling determination of the velocity profile inside the measurement volume. Two fringe systems with contrary fringe spacing gradients are generated to determine the position as well as the velocity of passing tracer particles. Physically discriminating between the two measuring channels is done by a frequency-division-multiplexing technique with acousto-optic modulators. A frequency-doubled Nd:YAG laser and a fiber-optic measuring head were employed, resulting in a portable and flexible sensor. In the center of the measurement volume of approximately 1-mm length, a spatial resolution of approximately 5 microm was obtained. Spatially resolved measurements of the Blasius velocity profile are presented. Small velocities as low as 3 cm/s are measured. The sensor is applied in a wind tunnel to determine the wall shear stress of a boundary layer flow. All measurement results show good agreement with the theoretical prediction.     

Investigation of the influence of spatial coherence of a broad-area laser diode on the interference fringe system of a Mach-Zehnder interferometer for highly spatially resolved velocity measurements

Laser Doppler anemometry is a method for absolute velocity measurements that is based on a Mach-Zehnder interferometer arrangement and usually employs transverse fundamental-mode lasers. We employed inexpensive and powerful broad-area laser diodes and investigated ways in which an interference fringe system is influenced by the spatial coherence properties of a multimode beam. It was demonstrated that, owing to poor spatial coherence of the beam, interference is suppressed in the marginal regions of the intersection volume. Based on these results, a sensor for highly spatially resolved velocity measurements can be built. The inherent astigmatism of the broad-area diode is corrected by an arrangement of two crossed cylindrical lenses. An interference fringe system of length 200 microm and a relative variation in fringe-spacing of only 0.22% were demonstrated with light emitted from a broad-area laser diode with a 100 microm x 1 microm emitter size. Based on this principle a powerful, simple, and robust laser Doppler sensor has been achieved. Highly spatially resolved measurements of a boundary layer flow are presented.     

Determination of the axial velocity component by a laser-Doppler velocity profile sensor

We report about the determination of the axial velocity component by a laser Doppler velocity profile sensor that is based on two superposed fanlike interference fringe systems. Evaluation of the ratio of the Doppler frequencies obtained from each fringe system yields the lateral velocity component and the axial position inside the fringe system. Inclined particle trajectories result in chirped burst signals, where the change of the Doppler frequency in one burst signal is directly related to the axial velocity component. For one single tracer particle it is possible to determine (i) the lateral velocity component, (ii) the axial velocity component including the direction, and (iii) the axial position of the tracer trajectory. In this paper we present the measurement principle and report about results from simulation and experiments. An uncertainty of the axial velocity component of about 3% and a spatial resolution in the micrometer range were achieved. Possible applications of the sensor lie in three-component velocity measurements of flow fields where only one optical access is available.     

Laser-Doppler velocity profile sensor with submicrometer spatial resolution that employs fiber optics and a diffractive lens

We report a novel laser-Doppler velocity profile sensor for microfluidic and nanofluidic applications and turbulence research. The sensors design is based on wavelength-division multiplexing. The high dispersion of a diffractive lens is used to generate a measurement volume with convergent and divergent interference fringes by means of two laser wavelengths. Evaluation of the scattered light from tracers allows velocity gradients to be measured in flows with submicrometer spatial resolution inside a measurement volume of 700-microm length. Using diffraction optics and fiber optics, we achieved a miniaturized and robust velocity profile sensor for highly resolved velocity measurements.     

Measurement of spatial cross sections of ultrasound pressure fields by optical scanning means

The measurement of spatial cross sections of ultrasound pressure fields is an essential element of exposimetry of ultrasonic medical equipment. An optical technique is presented that allows the two-dimensional (2-D) determination of ultrasound pressure using an optical multilayer hydrophone in which a laser beam with suitable wavelength is partially reflected from a dielectric optical multilayer system. By detecting the change in reflectivity of the multilayer coating induced by the incident ultrasound, the pressure time waveform can be determined. A 2-D data acquisition covering an area of at least 15 mm x 5 mm was realized by two complementary approaches. A serial detection scheme was set up by scanning the sensing point across the area of interest by a micromechanically engineered scanning mirror and acquiring pressure time waveforms sequentially and pointwise. This allows the measurement of repeating ultrasonic waveforms with a spatial resolution of better than 70 microm and a minimal detectable pressure of 50 kPa (bandwidth: 50 MHz) in a few seconds. In an alternative approach exploiting the parallel processing capabilities of a charge-coupled devices (CCD) camera chip, the probe was strobe-illuminated by a large-diameter collimated beam of a pulsed laser diode. The 2-D pressure distribution at a particular moment was derived from captured reflectivity distributions with a spatial resolution of 75 microm. By successive delaying of the laser pulse with respect to the ultrasound pulse, the complete 2-D pulse waveform was acquired with high spatial resolution. Measurement results on ultrasound fields from plane and focusing transducers are presented and compared to simulation results. Individual advantages and drawbacks of both approaches are discussed. A combined setup merging both detection schemes into a single device was developed and represents a milestone on the way toward constructing an optical ultrasound measuring camera.     

Real-time heterodyne imaging interferometry: focal-plane amplitude and phase demodulation using a three-phase correlation image sensor

A method of real-time heterodyne imaging interferometry using a three-phase correlation image sensor (3PCIS) is proposed. It simultaneously demodulates the amplitude and phase images of an incident interference pattern at an ordinary frame rate with good accuracy, thus overcoming the trade-off among measurement time, spatial resolution, and demodulation accuracy suffered in conventional interferometry. An experimental system is constructed with a 64x64 3PCIS camera operated at 30 frames/s and a two-frequency He-Ne laser with a beat frequency of 25 kHz. The results obtained for a scanning mirror and heated silicone oil confirm the proposed method.     

Fringe pattern analysis with a parametric method for measurement of absolute distance by a frequency-modulated continuous optical wave technique

Interferometry associated with an external cavity laser of long coherence length and broad wavelength tuning range shows promising features for use in measurement of absolute distance. As far as we know, the processing of the interferometric signals has until now been performed by Fourier analysis or fringe counting. Here we report on the use of an autoregressive model to determine fringe pattern frequencies. This concept was applied to an interferometric device fed by a continuously tunable external-cavity laser diode operating at a central wavelength near 1.5 microm. A standard uncertainty of 4 x 10(-5) without averaging at a distance of 4.7 m was obtained.     

Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera

A spatially resolved total internal reflection fluorescence correlation microscopy (TIR-FCM) system is constructed with an electron multiplying charge-coupled device (EMCCD) camera. The system was used to determine diffusion coefficients of lipid molecules in a planar lipid bilayer, and lipids and epidermal growth factor receptor (EGFR) proteins on cell membranes of Chinese Hamster Ovary (CHO) cells. The evaluation of the "cross talk" between neighboring pixels suggests that a higher degree of multiplexing can be achieved than was previously proposed [Kannan, B. et al. Anal. Chem. 2006, 78, 3444-51] using the same camera with a focused laser excitation. The best time resolution possible with this system is 4 ms for a region of interest comprising 20 lines in the CCD and is good enough to determine membrane diffusion in lipid bilayers and of membrane proteins in living cells. In this work, using a TIR-FCM setup, 1600 autocorrelation functions were measured simultaneously with a time resolution of 4.8 ms. This area corresponds to a 40 x 40 pixel region of interest with a dimension of 11.3 x 11.3 microm2 and is sufficiently large to allow the measurement of the lower membrane of a whole cell simultaneously.     

Analysis of a spatially dispersive displacement sensor utilizing an AlGaInP chip

We present a demonstration and analysis of an industrialized design of a spatially dispersive displacement sensor, which is composed of an AlGaInP gain chip in visible range, optical assembly, and a spectrum analyzer. The sensor utilizes the spatial dispersion of focus from the optical assembly and wavelength spectrum's deviation induced by the displacement of the target. As a result, the sensor delivers a quick and simple way of measuring displacement. By adapting the magnification and resolution of the optical assembly, a displacement sensor with a middle measurement range, ~10 microm, was obtained. However, we should note that 25 nm resolution is limited by the bandwidth and temperature fluctuation of the gain chip.     

Miniature rainbow schlieren deflectometry system for quantitative measurements in microjets and flames

Recent interest in small-scale flow devices has created the need for miniature instruments capable of measuring scalar flow properties with high spatial resolution. We present a miniature rainbow schlieren deflectometry system to nonintrusively obtain quantitative species concentration and temperature data across the whole field. The optical layout of the miniature system is similar to that of a macroscale system, although the field of view is smaller by an order of magnitude. Employing achromatic lenses and a CCD array together with a camera lens and extension tubes, we achieved spatial resolution down to 4 mum. Quantitative measurements required a careful evaluation of the optical components. The capability of the system is demonstrated by obtaining concentration measurements in a helium microjet (diameter, d=650 microm) and temperature and concentration measurements in a hydrogen jet diffusion flame from a microinjector (d=50 microm). Further, the flow field of underexpanded nitrogen jets is visualized to reveal details of the shock structures existing downstream of the jet exit.     

Raman microspectroscopy: a comparison of point, line, and wide-field imaging methodologies

Three different Raman microspectroscopic imaging methodologies using a single experimental configuration are compared; namely, point and line mapping, as representatives of serial imaging approaches, and direct or wide-field Raman imaging employing liquid-crystalline tunable filters are surveyed. Raman imaging data acquired with equivalent low-power 514.5-nm laser excitation and a cooled CCD camera are analyzed with respect to acquisition times, image quality, spatial resolution, intensity profiles along spatial coordinates, and spectral signal-to-noise ratios (SNRs). Point and line mapping techniques provide similar SNRs and reconstructed Raman images at spatial resolutions of approximately 1.1 microm. In contrast, higher spatial resolution is obtained by direct, global imaging (approximately 313 nm), allowing subtle morphological features on test samples to be resolved.     

Construction of a high-resolution moiré interferometer for investigating microstructural displacement fields in materials

A high-magnification moiré interferometer has been constructed with a spatial resolution of the order of 1 microm to measure the local in-plane displacement field associated with a material's microstructure. Laser illumination passes through phase-stepping optics and is delivered to the microscope head by polarization-preserving single-mode optical fibres. The head itself is a compact unit consisting of collimating optics, an objective lens and a charge coupled device (CCD) camera. Thin-phase gratings are cast onto the sample surface with a compliant epoxy resin and coated with ca. 5 nm of gold to enhance the fringe contrast and reduce speckle noise. By switching between the laser illumination and white-light illumination, the underlying microstructure is viewed in exact registration with the measured displacement fields. The application of the instrument is illustrated here by visualization of displacement fields in polymer-bonded explosives (PBXs) during deformation to failure. PBXs are highly filled polymers consisting of up to 95% by weight crystalline explosive bound in a variety of polymeric binders. The mechanical properties of PBXs are highly dependent on the microstructure, and moiré interferometry is an ideal tool for investigating the relationship between the 1-100 microm sized crystals and the displacement fields. Methods such as this are required if computer models of inhomogeneous materials are to be accurately validated.     

动态波前相位的高分辨率测量

动态波前相位信息测量是大气光学，气动光学和激光技术等领域的重要实验手段。提出了一种具有高的时间和空间分辨率以及长的测量持续时间的动态波前相位测量方法。应用Ｈａｒｔｍａｎｎ－Ｓｈａｃｋ波前传感器获得高空间分辨率的相位信息，采用高帧频ＣＣＤ摄象机获得高时间分辨率图象数据。     

Study of miscible and immiscible flows in a microchannel using magnetic resonance imaging

Magnetic resonance imaging (MRI) is a noninvasive technique that can be used to visualize mixing processes in optically opaque systems in up to three dimensions. Here, MRI has been used for the first time to obtain both cross-sectional velocity and concentration maps of flow through an optically opaque Y-shaped microfluidic sensor. Images of 23 micromx23 microm resolution were obtained for a channel of rectangular cross section (250 micromx500 microm) fed by two square inlets (250 micromx250 microm). Both miscible and immiscible liquid systems have been studied. These include a system in which the coupling of flow and mass transfer has been observed, as the diffusion of the analyte perturbs local hydrodynamics. MRI has been shown to be a versatile tool for the study of mixing processes in a microfluidic system via the multidimensional spatial resolution of flow and mass transfer.     

Enhanced geometrical superresolved imaging with moving binary random mask

In this paper, we address the geometrical resolution limitation of an imaging sensor caused by the size of its pixels yielding insufficient spatial sampling of the image. The spatial blurring that is caused due to inadequate sampling can be resolved by placing a two-dimensional binary random mask in an intermediate image plane and shifting it along one direction while keeping the sensor as well as all other optical components fixed. Out of the set of images that are captured, a high resolution image can be decoded. In addition, this approach allows improved robustness to spatial noise.     

Hydrodynamic flow profiling in microchannel structures by single molecule fluorescence correlation spectroscopy

In this paper we demonstrate high spatial resolution hydrodynamic flow profiling in silicon wafer based microchannels using single molecule fluorescence correlation spectroscopy (FCS). We have used confocal fluorescence microscopy to detect single tetramethylrhodamine (TMR-4-dUTP) biomolecules traversing a approximately 1 fL volume element defined by an argon laser beam focus. By elevating a (approximately 10(-10) M) reservoir of diluted analyte, a continuous hydrodynamic flow through the microstructure could be accomplished. The microchannel was then scanned with a diffraction-limited focus in approximately 1-microm steps in both the vertical and the horizontal directions to determine the flow profile across a 50 x 50 microm2 channel. The flow profile measured was parabolic in both dimensions, thereby showing a Poiseuille laminar flow profile. Future microstructures can hereby be nondestructively investigated with the use of high spatial resolution confocal correlation microscopy.     

Hybrid method combining orthogonal projection Fourier transform profilometry and laser speckle imaging for 3D visualization of flow profile

This paper reports a straightforward technique for three-dimensional (3D) visualization of a flow profile by a hybrid algorithm combining Fourier transform orthogonal fringe projection and laser speckle imaging techniques. The use of orthogonal projection aims to suppress the zero order allowing surface reconstruction with high spatial resolution and accuracy while analyzing the intensity fluctuations of diffuse backscattered laser light providing 2D flow information. Once both are achieved, 3D flow visualization can be displayed. The method is experimentally validated first with a plastic tube filled with scattering liquid (milk) running at various controlled flow rates and then with the tube embedded under scattering layers (chicken breast) of varying thickness. The system includes a single, common camera, a commercial projector (profilometry channel), a laser light source (flow channel), and a computer station. In addition, orthogonal projection processing was combined with Hilbert transform, increasing the visualization and resolution of the measured flow profile.     

Digital demodulation system for low-coherence interferometric sensors based on highly birefringent fibers

A new type of demodulation system for low-coherence interferometric sensors based on highly birefringent fibers is presented. The optical path delay introduced by the sensor is compensated in four detection channels by quartz crystalline plates of appropriate thickness. The system can be used to decode a single-point sensor with a resolution of 2.5 x 10(-3) or two serially multiplexed sensors with decreased resolution. In a multiplexed configuration each sensor is served by two detection channels. By tilting the quartz plates, we can tune the initial phase shift between interference signals in successive channels to differ by pi/8 or pi/4, respectively, for a single-point or a multiplexed configuration. We transferred the sinusoidal intensity changes into digital pulses by appropriate electronic processing, which eventually allows for an unambiguous phase-shift measurement with a resolution of 1/8 or 1/4 of an interference fringe. The system performance for the measurement of hydrostatic pressure changes and simultaneous changes of hydrostatic pressure and temperature is demonstrated. The pressure sensors are based on side-hole fiber to ensure high sensitivity and an operation range of 2.4 MPa. A new configuration for temperature compensation of hydrostatic pressure sensors is proposed, which is better suited for dynamic pressure measurements. In this configuration the sensing and compensating fibers are located in the same compartment of the sensor housing.     

High-throughput nanohole array based system to monitor multiple binding events in real time

We have developed an integrated label-free, real-time sensing system that is able to monitor multiple biomolecular binding events based on the changes in the intensity of extraordinary optical transmission (EOT) through nanohole arrays. The core of the system is a sensing chip containing multiple nanohole arrays embedded within an optically thick gold film, where each array functions as an independent sensor. Each array is a square array containing 10 x 10 nanoholes (150 nm in diameter), occupying a total area of 3.3 mum x 3.3 mum. The integrated system includes a laser light source, a temperature-regulated flow cell encasing the sensing chip, motorized optics, and a charge-coupled detector (CCD) camera. For demonstration purposes, sensing chips containing 25 nanohole arrays were studied for their use in multiplexed detection, although the sensing chip could be easily populated to contain up to 20 164 nanohole arrays within its 0.64 cm2 sensing area. Using this system, we successfully recorded 25 separate binding curves between glutathione S-transferase (GST) and anti-GST simultaneously in real time with good sensitivity. The system responds to binding events in a concentration-dependent manner, showing a sharp linear response to anti-GST at concentrations ranging from 13 to 290 nM. The EOT intensity-based approach also enables the system to monitor multiple bindings simultaneously and continuously, offering a temporal resolution on milliseconds scale that is decided only by the camera speed and exposure time. The small footprint of the sensing arrays combined with the EOT intensity-based approach enables the system to resolve binding events that occurred on nanohole sensing arrays spaced 96 mum apart, with a reasonable prediction of resolving binding events spaced 56 mum apart. This work represents a new direction that implements nanohole arrays and EOT intensity to meet high-throughput, spatial and temporal resolution, and sensitivity requirements in drug discovery and proteomics studies.     

Laser monitoring of the flow velocity in lymphatic microvessels based on a spatiotemporal correlation of the dynamic speckle fields

A method and the corresponding experimental setup for the in vivo laser monitoring of temporal variations in the velocity and direction of flow in lymphatic microvessels are described. Experimental results on the laser monitoring of flow in the mesenteric microvessels of rat are presented. The method is based on an analysis of the statistical properties of the dynamic speckle fields and provides for a high spatial resolution. The results of determination of the lymph flow velocity by the proposed method agree well with analogous data of functional transmission microscopy.