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Juan Yang Chun Cui Wanzhen Feng Haifeng Zhao Wei Wang Keming Dong 《International Journal of Food Science & Technology》2017,52(7):1623-1631
The influence of protein hydrolysates of salt duck egg white (PHSDEW) hydrolysed by acid protease on the quality of Jinga Shrimp (Metapenaeus affinis) was analysed. Shrimp immersed in 5% PHSDEW with degree of hydrolysis (DH 3%) had the highest values of cooking yield, water retention, moisture and texture with the lowest cooking loss when compared to which immersed in 0.4% NaCl, 3.5% mixed phosphates and other PHSDEW with different DH (1%, 2% and 5%) (P < 0.05). This treatment made muscle fibres retain the most appropriate compact and regular microstructure and improved the thermal stability of shrimp protein as well (P < 0.05). SDS‐PAGE protein patterns revealed shrimp protein would dissolve out during immersion, while only could PHSDEW infiltrate into shrimp muscle. It suggested PHSDEW would be developed as a new type of water retention agent replaced phosphate used in shrimp or other seafoods, which will decrease the waste of salt duck egg white. 相似文献
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Rattanasatheirn N Benjakul S Visessanguan W Kijroongrojana K 《Journal of food science》2008,73(1):S31-S40
ABSTRACT: Effects of freshness and deveining on some properties, translucence, and microstructure of Pacific white shrimp ( Litopenaeus vannamei ) soaked in 2.5% NaCl containing different phosphates were studied. Shrimp soaked in all solutions had increases in weight gain and cooking yield with lowered cooking loss, compared with the control ( P < 0.05). However, efficacy of mixed phosphates in quality improvement of ice-stored shrimp was lower than fresh shrimp. Deveining resulted in increased weight gain and yield ( P < 0.05). Nevertheless, samples treated with phosphates became more translucent. Shrimp stored in ice for 7 d and treated with mixed phosphates were generally more translucent than fresh counterparts ( P < 0.05). Shrimp soaked in 2.5% NaCl containing 0.875% sodium acid pyrophosphate (SAPP) and 2.625% tetrasodium pyrophosphate (TSPP) were generally less translucent and had high weight gain and cooking yield along with low cooking loss. The microstructure study revealed that the muscle fibers were less attached with the loss of Z-disks after being treated with mixed phosphates. Cooked meats of fresh shrimp and ice-stored shrimp had more compact fiber arrangement with the shrinkage of sarcomere compared with raw samples. Disintegration was observed at the M-line in ice-stored shrimp treated with mixed phosphates after cooking, while such a phenomenon was not found in the cooked fresh sample treated with phosphates. T max and enthalpy of both myosin and actin peaks shifted to lower values when shrimp were treated with mixed phosphates ( P < 0.05). Those changes were generally more pronounced in ice-stored shrimp. Therefore, freshness and deveining process had an impact on the quality of Pacific white shrimp treated with phosphates. 相似文献
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目的 以黄鲫蛋白肽(Half-fin anchovy hydrolysate peptides,HAHp)为基料复配抗坏血酸和茶多酚制备南美白对虾多酚氧化酶(Polyphenol oxidase,PPO)复合生物抑制剂(Composite biological inhibitor,CBI)。方法 以南美白对虾PPO抑制率为检测指标,分别研究HAHp、抗坏血酸、茶多酚、柠檬酸和EDTA-2Na单独抑制PPO活性,选取HAHp、抗坏血酸和茶多酚进行三因素三水平正交优化实验优化CBI配方组成,通过酶促动力学研究CBI对南美白对虾PPO的抑制模式。结果 HAHp、抗坏血酸、茶多酚、柠檬酸和EDTA-2Na对南美白对虾PPO都有一定的抑制作用,且有一定的剂量依赖性,但单一抑制剂对PPO的抑制作用有限。HAHp与天然抗氧化剂抗坏血酸和茶多酚复配,经(L9 33)正交试验优化CBI的最佳配方含有0.7%HAHp、0.02%抗坏血酸和0.20%茶多酚。经测定CBI对南美白对虾PPO抑制率达到94.71±0.46%,酶促动力学结果表明CBI是一种混合型抑制剂,通过降低南美白对虾PPO对底物亲和力实现抑制PPO活力。结论 以HAHp为基料复配抗坏血酸和茶多酚制备的CBI能够有效地抑制南美白对虾PPO活性,有望开发成一种防虾黑变的天然抑制剂,为南美白对虾天然保鲜剂的研究奠定了理论基础。 相似文献
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腌制方法对鸭蛋黄成分变化及品质影响 总被引:2,自引:0,他引:2
研究了鸭蛋在草木灰包裹法和盐水浸泡法腌制过程中,水分含量、盐分含量、R值与Q值,蛋黄总脂肪、可溶性蛋白质和巯基含量的变化以及成熟(7周)后两者蛋黄的感官品质。7周内,随腌制时间的延长,浸泡法鸭蛋黄水分含量明显低于包裹法鸭蛋黄,盐分含量却高于包裹法;蛋清R值高于蛋黄,灰蛋的R值、Q值都低于盐水蛋。第7周,灰蛋和盐水蛋的Q值上升为0.88和0.90;脂肪占湿重的含量上升至48.45%和50.67%,而腌制期总脂肪量是没有变化的;可溶性蛋白质含量增加至6.75%和6.08%;表面巯基和游离巯基下降至0.31、0.28mmol/g蛋黄和1.68、1.34mmol/g蛋黄。灰蛋蛋黄在形态、滋味和气味方面明显优于盐水蛋,感官评分分别为88.9和78.65。 相似文献
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Mu H Chen H Fang X Mao J Gao H 《Journal of the science of food and agriculture》2012,92(10):2177-2182
BACKGROUND: Shrimp is a very perishable product and postmortem changes occur rapidly. Sulfiting agents were once and are still widely used as a preservative in the shrimp industry. However, the application of sulfite in shrimp may pose a risk to human health. Thus development of a natural preservative as a sulfite alternative to extend the shelf life of Pacific white shrimp is urgently needed. RESULTS: The effects of cinnamaldehyde essential oil (1 and 5 g kg?1) on the shelf life of Pacific white shrimp stored at 4 °C were investigated. As the concentration of cinnamaldehyde increased, residual polyphenoloxidase (PPO) enzyme activity decreased. Kinetic analysis showed that cinnamaldehyde was a noncompetitive inhibitor for the oxidation of L ‐DOPA (L ‐3,4‐dihydroxyphenylalanine) by PPO of Pacific white shrimp. Based on this study, shrimp treated with 5 g kg?1 cinnamaldehyde possessed the lowest aerobic plate count, total volatile basic nitrogen, and pH values in all treatments after 10 days of storage. According to the results of L*, cinnamaldehyde showed inhibitory activity toward the formation of melanosis. CONCLUSION: Treatment with cinnamaldehyde could improve the sensory properties and extend the shelf life of Pacific white shrimp to 8 days. Therefore, cinnamaldehyde could be used as a promising natural preservative for inhibiting melanosis and preventing the growth of microbes during the chilled storage of Pacific white shrimp. Copyright © 2012 Society of Chemical Industry 相似文献
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Chemical composition, textural properties, and microstructure of cooked duck egg salted by 2 methods (coating and immersing) were determined during 4 wk of salting. As the salting time increased, moisture content increased and salt content decreased for both cooked salted egg white and yolk. Oil exudation of cooked yolk and expressible water content of cooked egg white obtained from both salting methods increased as salting proceeded (P < 0.05). After cooking, oil exudation accompanied by the solubilized pigments, especially at the outer layer of yolk, was obtained. At week 3 of salting, egg yolk from coating method had the higher egg exudation than that from immersing method. As the salting times increased, the lower hardness, springiness, gumminess, chewiness, and resilience with higher adhesiveness and cohesiveness were generally found in cooked salted egg white (P < 0.05), irrespective of salting methods. Conversely, the hardness of cooked yolk increased continuously and reached the maximum at week 2 and 2 to 3 for immersing and coating method (P < 0.05), respectively. Confocal laser scanning micrographs revealed the smaller yolk granules with more release of free lipid in salted egg after heating, compared with the fresh counterpart. As visualized by scanning electron microscope, gel of cooked salted egg white was coagulum type with larger voids. Salting methods determined oil exudation in egg yolk and texture profile of egg white gel after cooking; however, those attributes were also governed by the salting time. PRACTICAL APPLICATION: Salted duck egg can be made by 2 methods (coating and immersing) affecting the characteristic of salted egg white and yolk after cooking. Desirable cooked salted egg having the red yolk with hardness and high oil exudation could be obtained when salting was carried out for 3 and 4 wk for immersing and coating method, respectively. 相似文献
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为了提升玉米秸秆水解液(corn stalks hydrolysate,CSH)的可生化降解性,研究了蛋清蛋白(egg white protein,EWP)絮凝处理对CSH中木质素衍生物去除的影响,并与氯化铁、阳离子聚丙烯酰胺等常规絮凝剂的效果进行对比。结果表明,在初始CODCr浓度为40 g/L的CSH中使用EWP进行热絮凝处理(100℃下加热5 min),CSH中大分子木质素(以浊度计算)去除率达(76.87±3.00)%,显著优于氯化铁、阳离子聚丙烯酰胺的处理结果 (P <0.05)。同时,EWP热絮凝处理未造成糖、呋喃及弱酸等化合物明显损失。这是由于EWP通过疏水反应与木质素衍生物结合,进一步通过巯基-二硫键交联形成大分子化合物,促进了木质素衍生物从CSH中去除。 相似文献
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以南美白对虾为对象,研究温度变化和不同包装方式对物流过程中品质及组织形态的影响。实验模拟两种不同物流过程,同时采用两种不同包装方式,得4种模拟物流条件。测定模拟物流条件下不同包装的南美白对虾pH、菌落总数、挥发性盐基氮(TVB-N)、硫代巴比妥酸(TBA)、三甲胺(TMA)和K值变化。结果表明,温度变化频率高的模型Ⅰ对虾的品质影响显著——储藏过程中温度波动越大,越频繁,越会加速南美白对虾的劣变程度,其pH、菌落总数、TVB-N、TBA、TMA和K值都会随之增加。空气包装和真空包装相比,后者能够更好的抑制南美白对虾的品质劣变。其中模型Ⅰ-空气包装劣变最为严重,储藏结束时其pH为6.35,菌落总数为5.33 lg CFU/g,TVB-N上升至28.47 mg/100 g,TBA上升至3.56 mg MDA/kg,TMA则为5.14 mg/100 g。而模型Ⅱ-真空中的虾肉品质保持较良好状态,直到储藏结束其pH6.27,菌落总数为4.57 lg CFU/g,TVB-N上升至20.85 mg/100 g,TBA只上升到0.16 mg MDA/kg,TMA则为2.95 mg/100 g。结果表明,减少冷链物流中温度变化和真空包装对南美白对虾品质的劣化具有减缓作用。 相似文献
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可食性涂膜对南美白对虾微冻冷藏的影响 总被引:1,自引:0,他引:1
为改善南美白对虾易于黑变及货架期短的问题,采用M1(4-己基间苯二酚(4-HR)0.05g/L+壳聚糖1.5g/L+乳酸链球菌素(Nisin)0.2g/L)和M2(4-己基间苯二酚(4-HR)0.05g/L+酪蛋白酸钠1.5g/L+聚丙烯酸钠0.5g/L+乳酸链球菌素(Nisin)0.2g/L)两种保鲜剂配方对南美白对虾涂膜保鲜,(-3±1)℃条件贮藏。结果表明:相对于对照组,南美白对虾经M1和M2两种保鲜剂处理后,在(-3±1)℃贮藏过程中菌落总数、挥发性盐基氮(TVB-N)和丙二醛(MAD)增加缓慢,黑变被有效抑制。M1组有最佳抑菌效果,M2组有最佳的感官评分,M1和M2两种保鲜剂均可将货架期延长1倍多。 相似文献
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通过将不同比例的热处理蛋清粉添加到小麦粉中,研究热处理蛋清粉对小麦面团及馒头品质的影响。结果表明:随着热处理蛋清粉的添加,混合面团吸水率明显下降,面团形成时间和稳定时间有所增加,且稳定时间最长至13.10min;添加了热处理蛋清粉混合粉的峰值黏度逐渐上升;当添加量为4%时,混合面团的拉伸距离和拉伸强度最大,分别提高了5.6%、75%;随着热处理蛋清粉添加量的增加,α-螺旋和β-折叠先上升后下降;添加热处理蛋清粉可增强面筋蛋白包裹淀粉颗粒的能力;热处理蛋清粉的添加使馒头的硬度、胶着度及咀嚼性增大;当热处理蛋清粉添加量为4%时,馒头比容增加了2.3%,且感官评价最好。 相似文献
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Avtar Singh Soottawat Benjakul Pornpot Nuthong Thummanoon Prodpran 《International Journal of Food Science & Technology》2021,56(6):2760-2767
Structural and elemental properties of dried Pacific white shrimp (WSS) (Litopenaeus vannamei) shell having the white spots were compared with non-white spot shrimp (NWSS) shell. Based on X-ray diffraction (XRD) analysis, both samples showed two characteristic peaks representing chitin. However, WSS sample had two additional major peaks at 2θ of 26.65 and 29.53º, which were related to quartz and calcite, respectively. Scanning electron microscope coupled with energy-dispersive X-ray spectrophotometer (SEM-EDX) mapping showed the movement of various elements towards the exo- and endocuticle. The Raman spectroscopic study confirmed the formation of calcite crystals in the white spots. The looser chitin matrix was obtained for WSS as compared to that of NWSS as depicted in SEM micrographs. Therefore, the white spots developed in the shell were caused by the formation of calcite, which exhibited different structural arrangements and elemental positioning as compared to shrimp shell without white spots. 相似文献
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Liang Chen Wang Liao Jun Fang Si Qin Xiangyang Lu Jianping Wu 《Journal of Food Biochemistry》2020,44(6):e13220
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在最佳酶解条件下酶解瓜尔豆胶,并用HPGFC和HPLC分析酶解液的组成。取酶解液与蛋清蛋白进行接枝改性研究,固定反应温度为60℃,相对湿度为79%,改变底物配比,反应p H值和反应时间,制备不同的接枝产物并研究其凝胶性和持水性。同时,利用氨基酸分析,SDSPAGE分析和凝胶微观结构分析来表征接枝产物的凝胶性能。结果表明:瓜尔豆胶酶解液的主要成分是甘露低聚糖,几乎不含有单糖。为获得最高的凝胶性和持水性,制备接枝产物的最佳反应条件为:底物配比0.5%,p H值9.0,反应时间4 d,此改性蛋白粉在p H 4.0~10.0的范围内有较高的凝胶强度,且具有良好的储藏稳定性。随着改性时间的延长,改性蛋白粉中游离氨基和氨基酸含量减少。SDS-PAGE分析表明,瓜尔豆胶酶解液和蛋清蛋白在接枝反应的过程中,生成了相对分子质量较大的复合物。在SEM下观察,改性蛋白粉所成凝胶的网络结构比原蛋白粉更为紧密均匀。总的来说,瓜尔豆胶酶解液和蛋清蛋白的接枝反应能有效改善蛋清蛋白的凝胶性能。 相似文献
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研究冰温技术结合4- 己基间苯二酚(4-hexylresorcinol,4-HR)等保鲜剂对延缓虾类黑变和保持品质方面的效果。通过正交试验确定保鲜剂的配方(M),以冻结试验确定冰温贮藏温度,测定贮藏设备的温控范围,通过评定南美白对虾的黑变感官得分,测定多酚氧化酶(PPO)活力、挥发性盐基氮(TVB-N)、pH 值和菌落总数(TBC)等鲜度指标评价保鲜效果差异。结果表明:南美白对虾的冰温带为- 2.2~0℃,贮藏设备温度波动满足冰温技术要求,M 保鲜剂配方为0.01% 4- 己基间苯二酚+1.5% 柠檬酸+1% 抗坏血酸,冰温能显著减缓TVB-N 值、pH 值和TBC值增加速度,M 配方保鲜剂能有效抑制虾的多酚氧化酶活性和黑变,冰温技术结合M 配方保鲜剂保藏南美白对虾能互补二者优缺点,能显著减低黑变感官得分,PPO 活性、TVB-N、pH 值和TBC 的增长速度。比(4 ± 1)℃冷藏的货架期延长近1 倍。冰温技术结合保鲜剂能有效防止虾类黑变和延长品质货架期。 相似文献
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为提升麦草秸秆碱法预处理水解液(简称碱预处理液)的可生化降解性能,本研究利用蛋清蛋白(Egg White Proteins,EWPs)热絮凝工艺脱除碱预处理液中的木质素,并与EWPs常温絮凝工艺,以及分别以氯化铁(FeCl3)和聚氯化铝(PAC)为絮凝剂的传统絮凝工艺进行比较。结果表明,在碱预处理液浓度(以CODCr计)为8 g/L时,经EWPs热絮凝工艺处理,碱预处理液中大分子胶体木质素(以浊度计)去除率达70.6%±2.2%,小分子可溶性木质素(以总酚计)去除率达20.7±1.4%,与EWPs常温絮凝、FeCl3及PAC絮凝工艺相比均有所提高。EWPs热絮凝脱除木质素的机理探究结果表明,高温会引发EWPs变性,导致其蛋白结构发生变化,促进EWPs通过疏水及氢键作用与木质素絮凝结合;在加热过程中,EWPs通过分子间氢键作用及巯基-二硫键转换反应,进一步与木质素形成大分子絮体,促进了碱预处理液中大分子木质素及其衍生物的絮聚脱除。 相似文献