共查询到17条相似文献,搜索用时 93 毫秒
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生产手性分子的催化方法,通常包括有机金属催化、有机催化和生物催化。为了有效地创造新的活性药物成分,化学家正在对各种新技术和广袤的化学多样性进行不懈地深入研究。本文是根据C&EN休斯顿资深通讯记者ANN M.THAYER,发表在《化学与工程新闻》(2005年9月5日)上的”手性催化”一文编译而成。文中广泛介绍了有关催化反应、催化剂和实验技术的最新进展,对国内同行了解和把握这一领域的发展具有一定参考价值。 相似文献
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《制药原料及中间体信息》2006,(5):31-34
传递手性给一个分子有很多种不同的途径,其技术大致分为以下三类:拆分、手性池和不对称转化。后一种技术是把前手性分子定量地转化成单一手性分子,这是制备化学家们最感兴趣的。现在有多条不对称转化技术可以使用,人们最感兴趣的是生物催化和金属催化。 相似文献
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手性化合物与生物催化剂 总被引:1,自引:0,他引:1
孙万儒 《精细与专用化学品》1999,7(5):6-8
手性化合物作为医药、农药、香料、功能性材料的前体、中间体或终产物在精细化工产品的生产中占有极其重要的地位。手性化合物的生物合成与拆分是利用统称为生物催化剂的酶促反应或微生物转化的高度底物、立体、位点、区域选择性,将化学合成的前体、潜手性化合物或外消旋衍生物转化成单一光学活性产物。 相似文献
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Maiko Tanabe Dr. Sonoko Ishino Prof. Masafumi Yohda Prof. Kosuke Morikawa Prof. Yoshizumi Ishino Dr. Hirokazu Nishida 《Chembiochem : a European journal of chemical biology》2012,13(17):2575-2582
DNA ligases catalyze the joining of strand breaks in duplex DNA. The DNA ligase of Pyrococcus furiosus (PfuLig), which architecturally resembles the human DNA ligase I (hLigI), comprises an N‐terminal DNA‐binding domain, a middle adenylylation domain, and a C‐terminal oligonucleotide‐binding (OB)‐fold domain. Here we addressed the C‐terminal helix in the OB‐fold domain of PfuLig by mutational analysis. The crystal structure of PfuLig revealed that this helix stabilizes a closed conformation of the enzyme by forming several ionic interactions with the adenylylation domain. The C‐terminal helix is oriented differently in hLigI when DNA is bound; this suggested that disruption of its interaction with the adenylylation domain might facilitate the binding of DNA substrates. We indeed identified one of its residues, Asp540, as being critical for ligation efficiency. The D540R mutation improved the overall ligation activity relative to the wild‐type enzyme, and at lower temperatures; this is relevant to applications such as ligation amplification reactions. Physical and biochemical analyses indicated that the improved ligation activity of the D540R variant arises from effects on the ligase adenylylation step and on substrate DNA binding in particular. 相似文献
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随着手性科学的快速发展,获取手性内酯的单一对映体具有重要的研究和应用价值.酶法合成手性化合物具有催化效率高、立体选择性强、条件温和等优点,已成为目前化工领域研究和应用的热点.综述了当前主要三类酶法制备手性内酯的途径及其应用. 相似文献
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手性药物的HPLC分析是药物分析中较新的研究领域,综述了近年来手性药物色谱分离的研究进展。对手性衍生化试剂法和手性固定相法的分离机理、检测方法、试剂种类和应用等作了介绍。 相似文献
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Structural Analysis Reveals the Substrate‐Binding Mechanism for the Expanded Substrate Specificity of Mutant meso‐Diaminopimelate Dehydrogenase 下载免费PDF全文
Dr. Weidong Liu Prof. Dr. Rey‐Ting Guo Dr. Xi Chen Zhe Li Dr. Xiuzhen Gao Dr. Chun‐Hsiang Huang Prof. Dr. Qiaqing Wu Dr. Jinhui Feng Prof. Dr. Dunming Zhu 《Chembiochem : a European journal of chemical biology》2015,16(6):924-929
A meso‐diaminopimelate dehydrogenase (DAPDH) from Clostridium tetani E88 (CtDAPDH) was found to have low activity toward the D ‐amino acids other than its native substrate. Site‐directed mutagenesis similar to that carried out on the residues mutated by Vedha‐Peters et al. resulted in a mutant enzyme with highly improved catalytic ability for the synthesis of D ‐amino acids. The crystal structures of the CtDAPDH mutant in apo form and in complex with meso‐diaminopimelate (meso‐DAP), D ‐leucine (D ‐leu), and 4‐methyl‐2‐oxopentanoic acid (MOPA) were solved. meso‐DAP was found in an area outside the catalytic cavity; this suggested a possible two‐step substrate‐binding mechanism for meso‐DAP. D ‐leu and MOPA each bound both to Leu154 and to Gly155 in the open form of CtDAPDH, and structural analysis revealed the molecular basis for the expanded substrate specificity of the mutant meso‐diaminopimelate dehydrogenases. 相似文献
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Dr. Andrea C. Kneuttinger Martin Winter Nadja A. Simeth Kristina Heyn Prof. Dr. Rainer Merkl Prof. Dr. Burkhard König Prof. Dr. Reinhard Sterner 《Chembiochem : a European journal of chemical biology》2018,19(16):1750-1757
The artificial regulation of proteins by light is an emerging subdiscipline of synthetic biology. Here, we used this concept to photocontrol both catalysis and allostery within the heterodimeric enzyme complex imidazole glycerol phosphate synthase (ImGP‐S). ImGP‐S consists of the cyclase subunit HisF and the glutaminase subunit HisH, which is allosterically stimulated by substrate binding to HisF. We show that a light‐sensitive diarylethene (1,2‐dithienylethene, DTE)‐based competitive inhibitor in its ring‐open state binds with low micromolar affinity to the cyclase subunit and displaces its substrate from the active site. As a consequence, catalysis by HisF and allosteric stimulation of HisH are impaired. Following UV‐light irradiation, the DTE ligand adopts its ring‐closed state and loses affinity for HisF, restoring activity and allostery. Our approach allows for the switching of ImGP‐S activity and allostery during catalysis and appears to be generally applicable for the light regulation of other multienzyme complexes. 相似文献
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简要介绍环氧化物水解酶的研究进展。重点介绍环氧化物水解酶的结构特点和催化特性,即核心结构和帽子结构两个功能性结构及三位一体的催化活性构象。最后介绍环氧化物水解酶在生物催化等精细化学品领域中的应用,并展望其广阔的应用前景。 相似文献
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Dr. Taejin Kim Amude M. Kassim Ajit Botejue Dr. Chen Zhang Jared Forte Dr. David Rozzell Dr. Mark A. Huffman Dr. Paul N. Devine Dr. John A. McIntosh 《Chembiochem : a European journal of chemical biology》2019,20(9):1129-1132
Reactions that were once the exclusive province of synthetic catalysts can increasingly be addressed using biocatalysis. Through discovery of unnatural enzyme reactions, biochemists have significantly expanded the reach of enzymatic catalysis to include carbene transfer chemistries including olefin cyclopropanation. Here we describe hemoprotein cyclopropanation catalysts derived from thermophilic bacterial globins that react with diazoacetone and an unactivated olefin substrate to furnish a cyclopropyl ketone, a previously unreported reaction for enzyme catalysts. We further demonstrate that the resulting cyclopropyl ketone can be converted to a key cyclopropanol intermediate that occurs en route to the anti-hepatitis C drug grazoprevir. 相似文献