壳聚糖GFP质粒纳米粒对肿瘤细胞的转染效率研究

用绿色荧光蛋白（GFP）基因做报告基因，制备壳聚糖GFP质粒纳米粒，体外转染人肝癌细胞Hep G2和肺癌细胞3.549，定性评价纳米粒的转染效率和细胞毒性。结果表明壳聚糖GFP质粒纳米粒能转染两种细胞并在表达GFP，但两种细胞的转染效率不同，且该纳米粒能抑制两种细胞生长。     

A new kind of magnetic targeting induction heating drug carrier and its physical and biological properties

Nano-carbon and iron composite―carbon-coated iron nanoparticles (CCINs) produced by carbon arc method can be used as a new kind of magnetic targeting induction heating drug carrier for cancer therapy. The structure and morphology of CCINs are studied by X-ray diffraction (XRD) and transmission electron microscope (TEM). Mossbauer spectra of these nanoparticles show that they contain only iron and carbon, without ferric carbide and ferric oxide. CCINs can be used as the magnetic drug carrier, with the effect...     

中贫氧化矿高梯度磁选试验研究

对白云鄂博中贫氧化矿进行了高梯度磁选试验，着重研究了磁感应强度、矿浆流速对磁选指标的影响．采用粗选（B=0．6T）-精选（B=0．15T）工艺，在矿浆流速5．6cm／s、矿浆浓度20％的条件下，选出的铁精矿品位为60．0％。回收率达到79．5％．     

提高腺病毒对肿瘤细胞转染效率的药物效果评价

为了解决在肿瘤基因治疗中腺病毒载体介导的转染细胞效率低的问题,高效、低毒的转染试剂为其提供了一条新途径.采用正十二烷基-β-D-麦芽糖苷(n-Dodecyl-β-D-Maltoside,DDM)、DC-Chol、SDS联合腺病毒e GFP-Ad转染肝癌细胞Hep G2、食管癌细胞EC109及人正常肝细胞L-02与食管正常细胞Het-1A,48 h后观察绿色荧光蛋白表达情况并计阳性细胞率,评价联合转染试剂处理提高e GFP-Ad转染肿瘤细胞的效率.实验结果表明,DDM(3μg/m L)、DC-Chol(1 000μg/m L)、SDS(0.5μg/m L)联合e GFP-Ad转染肿瘤细胞Hep G2和EC109的效率分别可达85%和78%、80%和76%、45%和41%,与单独e GFP-Ad相比,转染效率分别提高16倍和14.6倍、15倍和14.2倍、8倍和7.2倍;且与正常细胞相比,DDM能显著提高对肿瘤细胞的转染效率,二者差异具有高度统计学意义.作为非脂质体类试剂,DDM能显著提高腺病毒对肿瘤细胞的转染效率,为进一步提高腺病毒介导的肿瘤基因治疗提供有效方法与技术参考.     

Role of VEGF-A/C and CCR-7 in the enhanced metastasis of A549 cells induced by 2 and 4 Gy X-rays in vitro and in vivo

Radiotherapy is a common strategy in treating lung cancer.Accumulating evidence suggested that radiotherapy has the potential to promote the metastasis and invasion of carcinoma cells.In this study,we aimed to testify the role of vascular endothelial growth factor(VEGF)and C-C chemokine receptor-7(CCR-7)in the metastasis of human adenocarcinoma A549 cells in vivo and in vitro.Nude mice were injected with A549 cells irradiated by 0,2 and 4 Gy X-rays,respectively.Quantitative detections of VEGF-A/C and CCR-7 mRNA from lung sample were performed by real-time RT-PCR.Small interfering RNA(siRNA)transfection technology was further used to testify the role of the genes in the metastasis of A549 cells.VEGF and CCR-7mRNA could only be detected 10 weeks post injection in vivo when visible tumor foci scattered in lung.In addition,VEGF-A/C mRNA expressed significantly higher in mice injected with A549 cells irradiated by 2 and 4 X-rays than those with 0 Gy X-rays irradiation.On the other hand,A549 cells with or without X-rays irradiation transfected with VEGF siRAN and CCR-7 siRNA showed a dramatic decrease of invasiveness compared to normal A549 cells with or without irradiation.The migration indexes were?0.7,?0.48,?0.34 and?0.32 for A549 cells with CCR-7 siRNA,VEGF siRNA,X-rays combined CCR-7 siRNA and X-rays combined VEGF-siRNA respectively.These results demonstrated that X-rays could promote the metastasis of A549 cells,and VEGF-A/C and CCR-7 mRNA were closely related to the metastasis of A549 cells in vivo and in vitro.     

阳离子脂质体的细胞毒性

为了解决在肿瘤基因治疗中腺病毒载体介导的转染细胞效率低的问题,高效、低毒的转染试剂为其提供了一条新途径.采用正十二烷基-β-D-麦芽糖苷（n-Dodecyl-β-D-Maltoside,DDM）、DC-Chol、SDS联合腺病毒eGFP-Ad转染肝癌细胞HepG2、食管癌细胞EC109及人正常肝细胞L-02与食管正常细胞Het-1A,48 h后观察绿色荧光蛋白表达情况并计阳性细胞率,评价联合转染试剂处理提高eGFP-Ad转染肿瘤细胞的效率.实验结果表明,DDM（3 μg/mL）、DC-Chol（1 000 μg/mL）、SDS（0.5 μg/mL）联合eGFP-Ad转染肿瘤细胞HepG2和EC109的效率分别可达85%和78%、80%和76%、45%和41%,与单独eGFP-Ad相比,转染效率分别提高16倍和14.6倍、15倍和14.2倍、8倍和7.2倍;且与正常细胞相比,DDM能显著提高对肿瘤细胞的转染效率,二者差异具有高度统计学意义.作为非脂质体类试剂,DDM能显著提高腺病毒对肿瘤细胞的转染效率,为进一步提高腺病毒介导的肿瘤基因治疗提供有效方法与技术参考.

     

HSV-tk联合GCV自杀系统对肝癌细胞株Huh-7杀伤作用的研究

单纯疱疹病毒胸苷激酶(herpessimplex virus-thymidine kinase,HSV-tk)基因,是目前研究比较多的自杀基因,其与前体药物丙氧鸟苷(ganciclovir,GCV)构成HSV-tk/GCV自杀基因系统.本实验将携带HSV-tk基因的慢病毒转染Huh-7细胞,通过报告基因反映慢病毒的转染效率;基因组PCR及RT-PCR检测转染Huh-7细胞中的HSV-tk基因的整合及表达;MTT检测不同浓度药物处理后的细胞存活率;最后HSV-tk+与HSV-tk-的Huh-7细胞以不同比例混合,MTT测定GCV作用后细胞的存活率.结果表明,HSV-tk/GCV系统作用于Huh-7细胞后,细胞出现明显的凋亡现象,旁观者效应比较明显.     

聚酰基硫脲基因输送体系的构建与评价

基于新型树枝状大分子聚酰基硫脲(PATU),通过巯基-烯Michael加成反应得到表面N, N-二甲基半胱胺修饰的聚酰基硫脲(PATU-DMCA). 核磁结果表明：PATU-DMCA树枝状结构正确且完整. 琼脂糖凝胶电泳及动态光散射粒径测定结果表明：PATU-DMCA在低代数、低氮磷摩尔比条件下可以有效包载DNA,形成粒径为60~100 nm、Zeta电位为10~20 mV的纳米复合物. 在人宫颈癌细胞HeLa和人肺癌细胞A549上,低氮磷摩尔比的PATU-DMCA的无血清转染效率优于经典的树枝状大分子基因载体聚酰胺胺,而两者细胞毒性相当. HeLa上的亚细胞分布结果表明：纳米复合物在细胞水平的转运过程基本包括黏附细胞膜、内吞入胞、进入溶酶体,通过“质子海绵”效应从溶酶体逃逸后进入细胞质及细胞核进行转染. PATU-DMCA以及PATU本身在基因输送中均具有很大的潜力.     

Micaceous iron oxide prepared from pyrite cinders by hydrothermal method

Micaceous iron oxide (MIO) with a hexagonal flaky shape was prepared by hydrothermal method. The ferric hydroxide used as precursor was obtained by an acidic leaching solution of pyrite cinders reacting with ammonia solution. The optimal experimental conditions for preparing micaceous iron oxide were investigated by orthogonal experiments. Micaceous iron oxide can be successfully prepared when optimal parameters of total iron concentration of 2.0 mol/L, pH value of 8, n(Fe²⁺)/n(Fe³⁺) of 0.1, mass of seed crystal of 1 g, reaction temperature of 260 °C and reaction time of 30 min are applied. X-ray diffractometry (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and selected area electron diffractometry (SAEM) were adopted to characterize the hydrothermal products prepared under optimal conditions. The results indicate that highly crystallized α-Fe₂O₃ hexagonal flakes, about 1.0–1.5 μm in diameter and 0.1 μm in thickness, are prepared. Furthermore, the quality of micaceous iron oxide prepared can meet the required characteristics of micaceous iron oxide pigments for paints (ISO10601—2007).     

Extracting indium and preparing ferric oxide for soft magnetic ferrite materials from zinc calcine reduction lixivium

A hydrometallurgical process for indium extraction and ferric oxide powder preparation for soft magnetic ferrite material was developed.Using reduction lixivium from high-acid reductive leaching of zinc oxide calcine as raw solution,copper and indium were firstly recovered by iron powder cementation and neutralization.The recovery ratios of Cu and In are 99% and 95%,respectively.Some harmful impurities that have negative influences on magnetic properties of soft magnetic ferrite material are deeply removed ...     

Facile preparation and formation mechanism of uniform silver nanoparticles ising OP-10 as emulsifier in reverse microemulsion

Using the polymerizable hydrophobic styrene monomer as the dispersion medium and the traditional nonionic surfactant OP-10 as emulsifier, stable silver nanoparticles of narrow size distribution were prepared by a reverse (w/o) microemulsion method. The powder X-ray diffraction (XRD) pattern indicated that the obtained silver nanoparticles were of face-centered cubic structure. The results of the transmission electron microscopy (TEM) show that the final silver nanoparticles are of spherical structure with an average diameter of 15.2 nm and of a Gaussian distribution. The internal high-ordered structure of silver nanoparticles was characterized by the field-emission high-resolution transmission electron microscopy (FEHRTEM), indicating that the silver is monocrystalline and it has only one nucleation site during the formation process of a nanoparticle. The time-resolved UV-visible absorption spectra was used to monitor the process of the reaction in situ. The results show that the concentration of silver nanoparticles increases but the size changes little and the morphology transforms from obvious ellipsoidal shape to nearly spherical shape during the process. The experimental results indicate that the droplets’ dynamic exchange which is closely related to the nature of surfactant film is the control factor of the kinetics. The dynamic exchange mechanism of silver nanoparticle formation is proposed to involve continual encounter of two separate droplets forming transient fused dimer in which the chemical reaction occurs followed by re-separation without combination. Attributed to the dual role of surfactant in the nanoparticle formation, tailored nanoparticles can be successfully synthesized in control in the premise of a certain stability of reverse microemulsion.     

Regulation mechanism of EM parameters in natural ferrite and its application in microwave absorbing materials

1 Introduction Microwave absorbing materials (MAM) refer to a kind of materials that can be used to absorb the emitted electromagnetic (EM) energy and to minimize the wave reflected in the direction of an energy radar receiver. When the EM wave incidents into MAM, it is readily absorbed, attenuated, and changed into heat or other energy. MAM are functional materials that possess special performance for absorbing EM wave. Along with the advancement in radar and microwave technologies, MA…     

Separation of hematite from banded hematite jasper(BHJ) by magnetic coating

The separation of iron oxide from banded hematite jasper(BHJ) assaying 47.8% Fe, 25.6% Si O2 and 2.30%Al2O3 using selective magnetic coating was studied. Characterization studies of the low grade ore indicate that besides hematite and goethite,jasper, a microcrystalline form of quartzite, is the major impurity associated with this ore. Beneficiation by conventional magnetic separation technique could yield a magnetic concentrate containing 60.8% Fe with 51% Fe recovery. In order to enhance the recovery of the iron oxide minerals, fine magnetite, colloidal magnetite and oleate colloidal magnetite were used as the coating material. When subjected to magnetic separation, the coated ore produces an iron concentrate containing 60.2% Fe with an enhanced recovery of56%. The AFM studies indicate that the coagulation of hematite particles with the oleate colloidal magnetite facilitates the higher recovery of iron particles from the low grade BHJ iron ore under appropriate conditions.     

Magnetic separation studies for a low grade siliceous iron ore sample

Investigations were carried out, on a low grade siliceous iron ore sample by magnetic separation, to establish its amenability for physical beneficiation. Mineralogical studies revealed that the sample consists of magnetite, hematite and goethite as major opaque oxide minerals where as silicates as well as carbonates form the gangue minerals in the sample. Processes involving combination of classification, dry magnetic separation and wet magnetic separation were carried out to upgrade the low grade siliceous iron ore sample to make it suitable as a marketable product. The sample was first ground and each closed size sieve fractions were subjected to dry magnetic separation and it was observed that limited upgradation is possible. The ground sample was subjected to different finer sizes and separated by wet low intensity magnetic separator. It was possible to obtain a magnetic concentrate of 67% Fe by recovering 90% of iron values at below 200 μm size.     

基因脉冲导入仪的研制

针对基因转移技术的发展，开发了LN－101基因脉冲导入仪，介绍了其组成基本原理及实现的方法，这一仪器的输出脉冲电压最大值为2500V，电流最大值为160A，其转染率是用化学方法所得不到的，该仪器具有高效率，低毒性，普适性和可控性，能够对多种类型的细胞实行基因导入。     

Isolation and characterization of acidophilic bacterium from Dongxiangshan Mine in Xinjiang Province, China

One bioleaching bacterium, named as strain DXS, was isolated from acid mine drainages (AMDs) of Dongxiangshan Mine of Hami, Xinjiang Province, China. The strain DXS is gram-negative and rod-shaped with a size of (0.40±0.05) μm×(1.3±0.5) μm. The optimal temperature and pH for growth are 30 ℃ and pH 2.0, respectively. It can grow autotrophically by using ferrous iron, elemental sulfur and NaS2O3 as sole energy sources. In the phylogenetic tree, strain DXS has similarity with Acidithiobacillus ferrooxidans typ...     

Magnetic biliary stent targeting to treat hepatoma combining with magnetic nanoparticles

To evaluate the effect of targeting to hepatoma treated by magnetic biliary stent combining with magnetic nanoparticle containing 5-fluorouracil （5-FU）, thirty-two nude mice modes with transplanted hepatoma were divided equally into four groups randomly. Experimental group received magnetic biliary stent and magnetic nanoparticles containing 5-FU. The tumor volume and pathomorphology of all groups was measured. The tumor control rate of the experimental group provided magnetic biliary stent wires and magnetic nanoparticles containing 5-FU is remarkably higher than three other control groups, showing significant curative effect. More apoptosis of tumor cells could be detected easily in experimental group. There are more apoptotic bodies and phagotrophic magnetic particle in apoptosis cells of experimental group under electron microscope. Magnetic biliary stent combining with magnetic nanoparticle containing 5-FU could inhibit the growth of hepatoma, and its curative effect is more remarkable than the traditional methods based on external magnetic fields.     

化学沉淀-磁絮凝深度快速除磷的研究

采用化学沉淀法研究Al2（SO4）3、FeCl3、CaCl2的药剂用量和pH值对除磷效果的影响,探讨非磁性悬浮物磁性接种的机理,并分析了铝、铁、钙的磷酸盐沉淀物由于加入磁种而变得易于沉降,特别是在磁场力作用下沉降速度加快的原因。结果表明,Al2（SO4）3、FeCl3、CaCl2是磷酸根离子的有效沉淀剂,在适当的药剂投加量和pH值条件下,废水经过处理后,剩余磷的浓度小于0．5mg／L;生成的铝、铁、钙的磷酸盐沉淀物颗粒与Fe3O4颗粒在某一pH值下表面电性相反,易发生相互凝聚;磁性接种的铝、铁、钙的磷酸盐沉淀物不仅变得更重,而且在磁场中还受到与重力方向相同的磁场力作用。