共查询到20条相似文献，搜索用时 15 毫秒
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Perozziello G La Rocca R Cojoc G Liberale C Malara N Simone G Candeloro P Anichini A Tirinato L Gentile F Coluccio ML Carbone E Di Fabrizio E 《Small (Weinheim an der Bergstrasse, Germany)》2012,8(18):2886-2894
This study aims to adoptively reduce the major histocompatibility complex class I (MHC-I) molecule surface expression of cancer cells by exposure to microfluid shear stress and a monoclonal antibody. A microfluidic system is developed and tumor cells are injected at different flow rates. The bottom surface of the microfluidic system is biofunctionalized with antibodies (W6/32) specific for the MHC-I molecules with a simple method based on microfluidic protocols. The antibodies promote binding between the bottom surface and the MHC-I molecules on the tumor cell membrane. The cells are injected at an optimized flow rate, then roll on the bottom surface and are subjected to shear stress. The stress is localized and enhanced on the part of the membrane where MHC-I proteins are expressed, since they stick to the antibodies of the system. The localized stress allows a stripping effect and consequent reduction of the MHC-I expression. It is shown that it is possible to specifically treat and recover eukaryotic cells without damaging the biological samples. MHC-I molecule expression on treated and control cell surfaces is measured on tumor and healthy cells. After the cell rolling treatment a clear reduction of MHC-I levels on the tumor cell membrane is observed, whereas no changes are observed on healthy cells (monocytes). The MHC-I reduction is investigated and the possibility that the developed system could induce a loss of these molecules from the tumor cell surface is addressed. The percentage of living tumor cells (viability) that remain after the treatment is measured. The changes induced by the microfluidic system are analyzed by fluorescence-activated cell sorting and confocal microscopy. Cytotoxicity tests show a relevant increased susceptibility of natural killer (NK) cells on microchip-treated tumor cells. 相似文献
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V. Staedtke M. Brähler A. Müller R. Georgieva S. Bauer N. Sternberg A. Voigt A. Lemke C. Keck J. Möschwitzer H. Bäumler 《Small (Weinheim an der Bergstrasse, Germany)》2010,6(1):96-103
The efficacy of antifungal treatment has been diminished by the biodistribution limitations of amphotericin B (AmB) due to its pharmacological profile, as well as the severe side effects it causes. A cellular drug‐delivery system, which incorporates human erythrocytes (RBCs) loaded with an AmB nanosuspension (AmB‐NS), is developed in order to improve antifungal treatment. AmB‐NS encapsulation in RBCs is achieved by using hypotonic hemolysis, leading to intracellular AmB amounts of 3.81 ± 0.47 pg RBC?1 and an entrapment efficacy of 15–18%. Upon phagocytosis of AmB‐NS–RBCs, leukocytes show a slow AmB release over ten days, and no alteration in cell viability. This results in an immediate, permanent inhibition of intra‐ and extracellular fungal activity. AmB‐NS–RBC‐leukocyte‐mediated delivery of AmB is efficient in amounts 1000 times lower than the toxic dose. This drug‐delivery method is effective for the transport of water‐insoluble substances, such as AmB, and this warrants consideration for further testing. 相似文献
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Jason R. McCarthy Ethan Korngold Ralph Weissleder Farouc A. Jaffer 《Small (Weinheim an der Bergstrasse, Germany)》2010,6(18):2041-2049
The synthesis and utility of a multimodal theranostic nanoagent based upon magnetofluorescent nanoparticles for the treatment of inflammatory atherosclerosis is described. These particles are modified with near‐infrared fluorophores and light‐activated therapeutic moieties, which allow for the optical determination of agent localization and phototoxic activation at spectrally distinct wavelengths. The resulting agent is readily taken up by murine macrophages in vitro and is highly phototoxic, with an LD50 of 430 pM . Intravenous administration results in the localization of the nanoagent within macrophage‐rich atherosclerotic lesions that can be imaged by intravital fluorescence microscopy. Irradiation of the atheroma with 650 nm light activates the therapeutic component and results in eradication of inflammatory macrophages, which may induce lesion stabilization. Importantly, these agents display limited skin photosensitivity, are highly efficacious, and provide an integrated imaging and therapeutic nanoplatform for atherosclerosis. 相似文献
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J. Matthias Löhr Rainer Heuchel Ralf Jesnowski Christine Wallrapp 《Advanced Engineering Materials》2009,11(8):B129-B135
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Matthew Gleeson Kevin O'Dwyer Sarah Guerin Daragh Rice Damien Thompson Syed A. M. Tofail Christophe Silien Ning Liu 《Advanced materials (Deerfield Beach, Fla.)》2020,32(46):2002873
Second-harmonic generation (SHG) is a nonlinear optical process that can provide disease diagnosis through characterization of biological building blocks such as amino acids, peptides, and proteins. The second-order nonlinear susceptibility tensor χ(2) of a material characterizes its tendency to cause SHG. Here, a method for finding the χ(2) elements from polarization-resolved SHG microscopy in transmission mode is presented. The quantitative framework and analytical approach that corrects for micrometer-scale morphology and birefringence enable the determination and comparison of the SHG susceptibility tensors of β- and γ-phase glycine microneedles. The maximum nonlinear susceptibility coefficients are d33 = 15 pm V−1 for the β and d33 = 5.9 pm V−1 for the γ phase. The results demonstrate glycine as a useful biocompatible nonlinear material. This combination of the analytical model and polarization-resolved SHG transmission microscopy is broadly applicable for quantitative SHG material characterization and diagnostic imaging. 相似文献
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Zijin Lin;Zahra Jiwani;Vahid Serpooshan;Haniyeh Aghaverdi;Phillip. C Yang;Aitor Aguirre;Joseph. C. Wu;Morteza Mahmoudi; 《Small (Weinheim an der Bergstrasse, Germany)》2024,20(41):2305940
Nanomedicine technologies are being developed for the prevention, diagnosis, and treatment of cardiovascular disease (CVD), which is the leading cause of death worldwide. Before delving into the nuances of cardiac nanomedicine, it is essential to comprehend the fundamental sex-specific differences in cardiovascular health. Traditionally, CVDs have been more prevalent in males, but it is increasingly evident that females also face significant risks, albeit with distinct characteristics. Females tend to develop CVDs at a later age, exhibit different clinical symptoms, and often experience worse outcomes compared to males. These differences indicate the need for sex-specific approaches in cardiac nanomedicine. This Perspective discusses the importance of considering sex in the safety and therapeutic efficacy of nanomedicine approaches for the prevention, diagnosis, and treatment of CVD. 相似文献
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Dehlinger DA Sullivan BD Esener S Heller MJ 《Small (Weinheim an der Bergstrasse, Germany)》2007,3(7):1237-1244
Multilayered structures composed of biomolecule-derivatized nanoparticles can be fabricated by electric-field-directed self-assembly. A microelectrode-array device facilitates the rapid parallel electrophoretic transport and binding of biotin and streptavidin fluorescent nanoparticles to specific sites on the microarray. Control of the current, voltage, and activation time of each of the 400-microarray electrodes allows a combinatorial approach to optimize nanoparticle binding. Under optimal conditions, nanoparticle layers form within 15 s of microelectrode activation, and the directed assembly of more than 50 alternate layers of nanoparticles is complete within an hour. The final multilayered structures are removed from the support by a relatively simple lift-off process. The electric-field process allows the parallel patterned assembly of multilayer structures using extremely low concentrations of nanoparticles and produces minimal nonspecific binding to unactivated sites. These results are significant for the development of rapid, maskless nanofabrication and hierarchical integration of biomolecular-derivatized nanocomponents into higher-order materials and devices. 相似文献
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Yu Hoshino Takeo Urakami Takashi Kodama Hiroyuki Koide Naoto Oku Yoshio Okahata Kenneth J. Shea 《Small (Weinheim an der Bergstrasse, Germany)》2009,5(13):1562-1568
Designed polymer nanoparticles (NPs) capable of binding and neutralizing a biomacromolecular toxin are prepared. A library of copolymer NPs is synthesized from combinations of functional monomers. The binding capacity and affinity of the NPs are individually analyzed. NPs with optimized composition are capable of neutralizing the toxin even in a complex biological milieu. It is anticipated that this strategy will be a starting point for the design of synthetic alternatives to antibodies. 相似文献
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Due to the significant role of clinical biomolecules in mediating biological activities and disease progressions, in situ analysis provides a great opportunity for understanding the molecular mechanisms of biological functions, facilitating medical diagnosis, clinical treatment, and prognosis. Among all the analysis methods, mass spectrometry (MS) methodologies exhibit unique features that make them exceptionally suitable for clinical tests. In this review, updated and essential developments of MS technologies, including laser‐based, spray‐based, plasma‐based, and intrasurgical MS techniques for clinical analysis are summarized. Moreover, further perspectives including design of portable sampling devices, introduction of miniature MS instrumentation, development of high‐throughput and multiplexed methodologies for clinical routine tests and point‐of‐care tests are proposed to achieve better advancements and practical applications of clinical MS. 相似文献
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Mingyue Cui;Lulu Qian;Ke Lu;Jinjin Liu;Binbin Chu;Xiaofeng Wu;Fenglin Dong;Bin Song;Yao He; 《Small (Weinheim an der Bergstrasse, Germany)》2024,20(43):2402101
Tumor-associated macrophages (TAMs) play a crucial function in solid tumor antigen clearance and immune suppression. Notably, 2D transitional metal dichalcogenides (i.e., molybdenum disulfide (MoS2) nanozymes) with enzyme-like activity are demonstrated in animal models for cancer immunotherapy. However, in situ engineering of TAMs polarization through sufficient accumulation of free radical reactive oxygen species for immunotherapy in clinical samples remains a significant challenge. In this study, defect-rich metastable MoS2 nanozymes, i.e., 1T2H-MoS2, are designed via reduction and phase transformation in molten sodium as a guided treatment for human breast cancer. The as-prepared 1T2H-MoS2 exhibited enhanced peroxidase-like activity (≈12-fold enhancement) than that of commercial MoS2, which is attributed to the charge redistribution and electronic state induced by the abundance of S vacancies. The 1T2H-MoS2 nanozyme can function as an extracellular hydroxyl radical generator, efficiently repolarizing TAMs into the M1-like phenotype and directly killing cancer cells. Moreover, the clinical feasibility of 1T2H-MoS2 is demonstrated via ex vivo therapeutic responses in human breast cancer samples. The apoptosis rate of cancer cells is 3.4 times greater than that of cells treated with chemotherapeutic drugs (i.e., doxorubicin). 相似文献
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Wenjing Wu;Haimei Li;Wenqi Chen;Yulin Hu;Zichen Wang;Wenyan She;Liang Huang;Yi Liu;Peng Jiang; 《Small (Weinheim an der Bergstrasse, Germany)》2024,20(37):2401299
The immunosuppressive tumor microenvironment (TME) reduces the chimeric antigen receptor (CAR) T-cell therapy against solid tumors. Here, a CAR T cell membrane-camouflaged nanocatalyst (ACSP@TCM) is prepared to augment CAR T cell therapy efficacy against solid tumors. ACSP@TCM is prepared by encapsulating core/shell Au/Cu2-xSe and 3-bromopyruvate with a CAR T cell membrane. It is demonstrated that the CAR T cell membrane camouflaging has much better-targeting effect than the homologous tumors cell membrane camouflaging. ACSP@TCM has an appealing synergistic chemodynamic/photothermal therapy (CDT/PTT) effect that can induce the immunogenic cell death (ICD) of NALM 6 cells. Moreover, 3-bromopyruvate can inhibit the efflux of lactic acid by inhibiting the glycolysis process, regulating the acidity of TME, and providing a more favorable environment for the survival of CAR T cells. In addition, the photoacoustic (PA) imaging and computed tomography (CT) imaging performance can guide the ACSP@TCM-mediated tumor therapy. The results demonstrated that the ACSP@TCM significantly enhanced the CAR T cell therapy efficacy against NALM 6 solid tumor mass, and completely eliminated tumors. This work provides an effective tumor strategy for CAR T cell therapy in solid tumors. 相似文献
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