共查询到20条相似文献,搜索用时 109 毫秒
1.
植烟土壤硫化细菌与反硫化细菌分布特点研究 总被引:1,自引:0,他引:1
利用选择性培养基,采用最大或然数法对其计数,对云南大理州主要植烟区土壤硫化细菌及反硫化细菌进行分离,以了解硫化细菌和反硫化细菌在烟草不同生长时期的动态变化。结果表明,剑川甸南、下庄、程官和弥渡4个烤烟主产区硫化细菌和反硫化细菌根际土细菌数大于田间土,硫化细菌根际与田间土之比为R:S=6~10:1,反硫化细菌的根土比约R:S=2.5~3.5:1,弥渡烤烟区细菌数量要明显大于其他3个烤烟区。永平和南涧地区田间土硫化细菌数比根际要高。土壤pH与土壤细菌含量呈负相关性,对反硫化细菌的影响要远远大于硫化细菌;根际硫化细菌与田间土硫化细菌呈显著正相关性r=1.00,根际与田间土反硫化细菌呈显著正相关性r=0.879。硫化细菌在成熟期含量较高,反硫化细菌数在团棵期含量较高。 相似文献
2.
在自然界中,细菌常以细菌生物膜的形式存在,其形成给公共卫生、食品安全、环境等诸多领域带来了不利影响。复杂的细菌生物膜结构为生理多样化的细菌细胞提供了保护屏障,细菌生物膜的形成是细菌感染及细菌抗药耐药性问题出现的主要驱动因素。噬菌体是细菌的天敌,细菌与噬菌体之间的共同进化迫使噬菌体开发特定的策略来克服生物膜防御屏障并杀死细菌。本文总结了细菌生物膜的组成、结构、形成过程及噬菌体对细菌生物膜的感染机制;综述了基于噬菌体及其衍生物对抗生物膜和耐药细菌感染的最新研究进展;最后,针对目前噬菌体疗法存在的问题进行了讨论,以期发现和更好地利用噬菌体的天然抗菌潜力,促进新型、高效的细菌生物膜检测及控制技术进一步发展。 相似文献
3.
4.
5.
乳酸菌细菌素是细菌在核糖体上合成的具有抗菌活性的多肽类物质,这些细菌素能杀灭或抑制引起食品腐败的细菌的繁殖,可作为天然的食品防腐剂在食品中应用。该文综述了乳酸菌细菌素的分类、国内外研究现状、抑菌特性及应用等方面的最新研究进展,并对乳酸菌细菌素未来研究趋势进行简要分析,对乳酸菌细菌素在食品中的应用有一定参考价值。 相似文献
6.
7.
8.
基于高通量测序技术巴彦淖尔市酸粥中细菌多样性分析 总被引:2,自引:0,他引:2
采用Illumina MiSeq高通量测序技术对采集自内蒙古自治区巴彦淖尔市的10份酸粥样品中的细菌多样性进行解析,同时基于细菌属水平对其进行围绕中心点的划分(PAM),并基于PICRUSt和BugBase软件对细菌的基因功能及表型进行预测。结果表明,所有酸粥样品中的细菌隶属于9个细菌门的55个属,样品间细菌多样性和丰度存在明显差异,但优势细菌门均为硬壁菌门(Firmicutes)和变形菌门(Proteobacteria),优势细菌属均为乳杆菌属(Lactobacillus)和醋杆菌属(Acetobacter)。所有酸粥样品可分为两个聚类,聚类I以醋杆菌属(Acetobacter )为主,聚类II以乳杆菌属(Lactobacillus)为主;两个聚类中细菌对于碳水化合物的利用和细菌的生长繁殖等存在显著差异(P<0.05),隶属于聚类I的酸粥样品中,其细菌的代谢和繁殖更加迅速。 相似文献
9.
糙米发芽过程中优势细菌分离鉴定 总被引:2,自引:4,他引:2
研究糙米在发芽过程中细菌总数变化,分离、纯化、鉴定发芽糙米中优势细菌;以营养琼脂为培养基,采用十倍稀释法分离、纯化发芽糙米中优势细菌,并采用16S rDNA测序与基因比对方法鉴定发芽糙米优势细菌。发芽过程中,细菌总数先减少再增加,在发芽完成时,细菌总数达7.08×108 cfu/g;分离到1株优势细菌,该细菌菌株与蜡样芽孢杆菌(Bacillus cereus)16S rDNA序列相似性为99.5%;所以,发芽糙米存在食品安全隐患,必须控制糙米发芽过程中微生物。 相似文献
10.
11.
报道了一种用1.5mL Ep管培养菌液,用滤纸片法筛选抗生物质产生菌的新型初筛方法。与传统方法相比具有工作量小,检测手段灵敏,特别适用于微好氧菌及兼性厌氧菌的筛选等特点。使用此法一天时间可以制备4000~5000个滤纸片样品,每个EP管至少可以检测对十种以上致病菌的抑菌效果,这些是传统的琼脂块法无法比拟的。使用此种筛选方法从泡菜水中检出抗生物质产生茵的比率高达71.3%。 相似文献
12.
该研究从清香型白酒大曲中筛选出一株对致病性尖孢镰刀菌(Fusarium oxysporum)拮抗效果最好的拮抗菌株,采用形态观 察、生理生化试验和16S rRNA序列分析对其进行鉴定,并绘制该菌株生长曲线、考察其培养液中的蛋白类及脂肽类物质的抑菌效 果。 结果表明,从清香型白酒大曲中筛选出一株对致病性尖孢镰刀菌拮抗效果最好的拮抗菌株,编号为P9,其抑菌圈直径达到20 mm 左右。 经鉴定,拮抗菌P9为贝莱斯芽孢杆菌(Bacillus velezensis);生长曲线结果表明,拮抗菌P9在第9小时生长速率达到最大;抑菌物 质提取实验表明,拮抗菌P9能分泌具有抑菌作用的蛋白类物质及脂肽类物质。 相似文献
13.
Yamamura S Morita Y Hasan Q Rao SR Murakami Y Yokoyama K Tamiya E 《Journal of Bioscience and Bioengineering》2002,93(6):595-600
A keratin-degrading bacterium was isolated from soil containing deer fur. An axenic culture of the keratin-degrading bacterium was obtained in liquid culture using a keratin enrichment technique. The isolated bacterium was gram negative and catalase- and oxidase-positive. Transmission electron microscopic observations showed that the bacterium was rod-shaped, 1.0-1.3 microm long and 0.7 microm in diameter. Phylogenetic analysis of 16S rDNA revealed that the new isolate has only 90.6% homology with Stenotrophomonas nitritireducens. Hence, this new bacterium was designated as Stenotrophomonas sp. D-1. The optimum temperature was determined to be 20 degrees C for maximum growth and keratinolytic enzyme production. Amino acid data, obtained after treating keratin powder with the supernatant culture, suggest that the major free amino acids resulting from keratin degradation are phenylalanine, tyrosine and valine. In addition, native chicken feather was degraded completely at 20 degrees C in 2.5 d by this bacterium. 相似文献
14.
乳酸菌在发酵香肠中的研究现状 总被引:2,自引:1,他引:2
本文综述了乳酸菌在发酵香肠中的研究现状,主要介绍了发酵香肠中常用的乳酸菌种类及作用,乳酸菌的作用机制,国内外研究现状,最后对其发展趋势进行了展望。 相似文献
15.
Salt-cured and dried salt-cured cod rehydrated using sterile water and equipment have a short shelf life at 4 degrees C due to high bacterial counts. The microbiota develops off-odours which partly can be described as musty, causing sensory rejection within 7-10 days of chilled storage. The microbiota composition was studied in a total of 38 samples obtained from 10 different, both commercial and laboratory produced, salt-cured and dried salt-cured cod products. The dominating bacterium, representing at least 90% of the total viable count in all products studied, was identified as belonging to the genus Psychrobacter; a Gram-negative, oxidase- and catalase-positive, nonpigmented, halotolerant, psychrotolerant, facultative aerobe and nonmotile bacterium. The morphology of the bacterium resembles coccobacilli and the cells occur most often in pairs. The bacterium was able to hydrolyze lipids, but not proteins. It did not produce H(2)S or TMA and the spoilage in rehydrated salt-cured and dried salt-cured cod is therefor different from what is observed in fresh cod. However, samples inoculated with Psychrobacter immobilis gave the same musty odour as spoiled control samples but earlier in the storage period and of a stronger intensity. In a field experiment, carried out to investigate the origin of the dominating bacterium, it was found that the microbiota in both sterile rehydrated commercially produced and laboratory (aseptically) produced salt-cured cod was dominated by this same bacterium. The bacterium was also isolated from cod skin mucus immediately after capture. The bacterium survived NaCl concentrations up to 25% (w/v) NaCl, stating its ability to survive during the salt-curing process. The dominating bacterium in rehydrated salt-cured and dried salt-cured cod seems to mainly originate from the fresh fish itself and not from contamination during processing. 相似文献
16.
为了提高草莓的附加值,研究多菌混合发酵酿造草莓果醋的工艺,主要考察原料糖度、温度和菌种量对草莓果醋产量和品质的影响,确定最佳的酿造工艺条件。结果表明,多菌混合发酵法酿造苹果醋的最佳工艺条件:原料糖度为18%,接菌种量为原料质量的10%,发酵温度为30℃,发酵周期16~18 d。证明多菌混合发酵法简化了工艺,是一种值得推广的果醋生产技术。 相似文献
17.
从沤麻水中,分离出多种菌株,经初筛、复筛获得4种好氧菌。用革兰氏染色后镜鉴,R-M26是黑曲酶;R-X80为芽孢杆菌;R—S76、R-S136都是链球状,革兰氏染色呈紫色。对选出的4种菌分别进行单菌和混菌脱胶,发现4种菌混合脱胶效果好,且脱胶效果提高了10%。 相似文献
18.
19.
20.
Silver ions can effectively kill bacteria due to their adsorption onto the negatively charged bacterial cell wall, thus deactivating cellular enzymes, disrupting membrane permeability, and ultimately leading to cell lysis and death. In this study, silver particles incorporated into hollow polyethylene terephthalate (PET) fibers are utilized as an antibacterial composition against the bacterium, Staphylococcus aureus. Also, a silver ion–bacterium model similar to the predator–prey model is established to analyze the inhibitory effect of silver ions on S. aureus. In contact with an aqueous solution, silver ions are released from hollow PET fibers into the solution. When a silver ion in an active medium is absorbed by a bacterium, the bacterium dies and the concentration of silver ions lowers in the medium. To keep the concentration of silver ions in the medium constant, another silver ion is released from the hollow fiber, making the silver ion–bacterium model much simpler to be solved analytically. This study shows that there is an optimal concentration of silver ions at which minimal cytotoxicity and maximal antibacterial efficacy can be achieved. To support this, experimental verification is also carried out in this study. 相似文献