QSTAR Pulsar028Ⅰ型串连四极杆飞行时间液质联用质谱仪简介及其应用

简要介绍QSTAR质谱仪的工作原理、性能特点,并从使用者的角度介绍该质谱所配备的各种离子源的特点及应用举例。     

气溶胶在线质谱仪及其在汽车尾气中的应用

汽车排放的颗粒气溶胶和有害气体严重影响着大气环境和人们的健康。我们研制了一台能够在线监测气溶胶粒径分布和气体组成成分的气溶胶在线质谱仪。此仪器具有响应时间快,性能稳定,自动化程度高和移动性好等特点。仪器采用了软电离方式,避免了碎片离子峰的形成,便于对污染环境中挥发性有机物的解析。实验结果表明仪器对挥发性有机物的检测下限为25ppb量级,并能实时测量尾气中气溶胶的浓度,在工厂废气以及污染场所气溶胶的测定方面有广阔的应用前景。     

三重四级杆质谱仪的安装应用和维护

质谱技术已经成为一种重要的现代分析鉴定技术,三重四级杆质谱,采用空间串联四级杆分析器,对选择离子分析具有较高灵敏度,应用广泛.建议专人负责质谱的安装,管理,应用及维护.维持稳定的电源,充足的液氮和氮气,及时清洗和维护,以保证质谱仪正常,稳定的工作.     

质谱仪离子导向装置的原理、应用和进展

离子导向装置（ion guide）是传输离子的重要手段。在质谱仪器中,离子导向装置是离子光学（ion optics）系统的重要组成部分,是联络处于低真空（或大气压）电离源和质量分析器的桥梁。离子导向装置不仅用于传输离子,还可降低离子的空间发散度和能量分散度,使离子最大限度到达质量分析器。本工作主要对一些常用的离子导向装置的原理、应用和进展进行了评述,并展望了未来的发展。     

TRITON同位素质谱仪磁场检修二例

TRITON是目前国际上顶尖的固体同位素质谱仪之一,测量准确度与分析精度优于0.0005%。由于结构复杂,线路众多,本文仅介绍磁场系统基本组成,并剖析二例典型故障,供同行参考。     

微波等离子体飞行时间质谱仪研制及元素检测应用研究

微波等离子体炬（microwave plasma torch,MPT）具有功耗低、操作方便、结构简单等优点,与质谱仪联用可快速分析元素。本文报道了一套自制的低功耗微波等离子体炬飞行时间质谱仪（MPT-TOF MS）及进样装置,并考察了维持气流速、载气流速、超截取锥电压、MPT炬焰位置及微波功率对元素检测的影响,系统地研究了该仪器的性能。结果表明,MPT-TOF MS具有耗气量少（氩维持气流速800 mL/min、氩载气流速400 mL/min、氮干燥气流速2 000 mL/min）、离子源功耗低（100 W）的优势,且线性范围覆盖5个数量级,分辨率高,锂、钠、钾、铷和铯元素的检出限分别为0.49、3.05、1.31、0.74、0.34μg/L。该仪器可用于盐湖中锂、钠、钾、铷及铯碱金属元素的快速检测,是电感耦合等离子体质谱（ICP-MS）元素检测方法的补充。     

MALDI—TOF质谱仪关键技术及进展

基质辅助激光解吸电离飞行时间质谱仪是当前研究蛋白质组不可缺少的质谱仪,已经得到广泛的应用,本文对这种仪器的结构原理、构造特点、关键技术和最新进展等作简要介绍。     

离子阱质谱仪小型化的最新研究进展

为适应科技发展的需要，质谱议的小型化已成为目前分析仪器发展的一个重要趋势，其中离子阱质谱仪的小型化取得了举世瞩目的成果。离子阱的小型化是通过应用小型真空泵、小型真空系统及较低的射频电压和简化阱结构（从双曲线离子阱到圆柱型离子阱再到矩形离子阱）来实现的。和其他形式的Paul离子阱一样，在外部离子注入模式下，圆柱形离子阱具有较低的捕获效率和较低的储存容量（对于商业尺寸的离子阱只能存储大约500个离子），尤其是在低射频电压工作条件下，阱尺寸减小时更是如此。为克服这些缺点，出现了一种新的离子阱质量分析器一矩形离子阱质量分析器。本文介绍了离子阱质量分析器的小型化原理，对其最新研究进展进行了评述。     

在线质谱仪在工业发酵过程优化与放大中的应用

本文主要介绍了在线质谱仪的特点以及在工业发酵过程优化与放大中的应用。在线质谱仪测量精度高、漂移小、分析速度快,可实现长期连续监测,在生物过程领域具有巨大的应用潜力。     

GC-C-IRMS气相色谱-燃烧-同位素质谱仪使用及改进

气相色谱仪-燃烧-稳定同位素比例质谱仪具有灵敏度高、检测周期短,方便等特点,在同位素自然丰度和示踪分析方面得到广泛应用。仪器的日常维护,是获得良好测试数据和延长仪器使用寿命的前提和基础。本文介绍了气相色谱仪-燃烧-稳定同位素比例质谱仪的工作原理和操作流程,并对使用过程中常出现的氧化管更换问题提出了应对策略。此外,还对气相色谱仪-燃烧-稳定同位素比例质谱仪使用过程中的日常维护和注意事项进行了阐述。     

The allure of mass spectrometry: From an earlyday chemist's perspective

This reminiscing review article is an account of the author's fascination and involvements with mass spectrometry from the perspective of an organic chemist with an interest in natural product chemistry. It covers a period from 1961 through the mid 1990s as mass spectrometry evolved form a novelty technique to become a most widely used analytical technique. Following a brief synopsis of my pathway to mass spectrometry, my research efforts in this field are presented with a focus mainly on evolving principles and technologies which I had personal involvements with. To provide historical perspectives, discussions of these developments are accompanied by brief outlines of the relevant state‐of‐the‐art, shedding light on the technical and conceptual challenges encountered during those early days in mass spectrometry. Examples are presented of my involvements with basic and applied research in mass spectrometry during graduate studies at Stanford University and close to three decade tenure in pharmaceutical research at Syntex Research. My basic research interests focused mainly on principles of electron ionization induced fragmentation mechanisms, with an emphasis on steroids and other model compounds. Extensive deuterium labeling evidence was used to determine the fragmentation mechanisms of the diagnostically significant ions in the spectra of numerous model compounds, uncovering examples of wide‐ranging hydrogen transfers, skeletal rearrangements, methyl and phenyl migrations, stereoselective fragmentations and low and high energy fragmentation processes. Depiction of the industrial research phase of my career includes comments on the pivotal role mass spectrometry played on advancing modern pharmaceutical research. Examples are presented of involvements with instrumental developments and a few select cases of applied research, including studies of bile mechanisms in vertebrates, identification of bisphenol‐A leaching from sterilized polycarbonate containers, high sensitivity TCDD analyses and other projects. Reflecting on my services for the mass spectrometry society, involvements with the co‐founding and 12 year chairing of the Asilomar Conference on Mass Spectrometry and founding of the Bay Area Mass Spectrometry regional MS discussion group, as part of my services for the mass spectrometry community, are presented in some detail. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 36:520–542, 2017     

生物质谱在蛋白质组学研究中的应用

本文简述了生物质谱的发展以及在蛋白质组学研究中的应用。     

傅立叶变换离子回旋共振质谱及其在药学领域的应用

傅立叶变换离子回旋共振质谱可以实现超高分辨率和质量精确度,在药物结构鉴定和元素分析中展现出强大的优势,并发展成为分析复杂混合物的强有力工具。本文简述了傅立叶变换离子回旋共振质谱的分析特性以及在药学领域的应用。     

Crosslinking combined with mass spectrometry for structural proteomics

The method of crosslinking combined with mass spectrometry is being gradually accepted as a technology enabling detailed structural information on proteins and protein complexes. Intrinsic challenges of the method, which have prevented its widespread use, are being progressively addressed by improvements in mass spectrometry instrumentation capabilities, by the development of new crosslinking reagents, and by the development of specialized software tools for processing of mass spectrometric crosslinking data. This review focuses on recent literature concerning the development of specialized crosslinking reagents and approaches for mass spectrometry‐based applications. Critical features of crosslinking reagents for optimum mass spectrometric performance, such as isotopic coding, cleavability, affinity groups, structure of the linkers, and reactive groups, are assessed. Requirements for the design of crosslinking reagents to make them well suited for mass spectrometric detection and analysis are summarized. © 2010 Wiley Periodicals, Inc. Mass Spec Rev 29:862–876, 2010     

Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research

Progress in proteomics research has led to a demand for powerful analytical tools with high separation efficiency and sensitivity for confident identification and quantification of proteins, posttranslational modifications, and protein complexes expressed in cells and tissues. This demand has significantly increased interest in capillary electrophoresis‐mass spectrometry (CE‐MS) in the past few years. This review provides highlights of recent advances in CE‐MS for proteomics research, including a short introduction to top‐down mass spectrometry and native mass spectrometry (native MS), as well as a detailed overview of CE methods. Both the potential and limitations of these methods for the analysis of proteins and peptides in synthetic and biological samples and the challenges of CE methods are discussed, along with perspectives about the future direction of CE‐MS. @ 2019 Wiley Periodicals, Inc. Mass Spec Rev 00:1–16, 2019.     

现代生物质谱及其应用

80年代以来,有机质谱获得突破性进展,其应用已跨入了生物大分子的研究领域,并迅速形成了一个新的分支学科生长点——生物质谱学。已经诞生的生物质谱计已在生命科学的研究中发挥着非常重要的作用。本文主要介绍基质辅助激光解吸电离/飞行时间质谱(MALDI/TOF MS)的构造、原理、功能及主要应用。