Correlation between sealer penetration into dentinal tubules and bond strength of two new calcium silicate-based and an epoxy resin-based,endodontic sealer

The relationship between sealer penetration into dentinal tubules and the bond strength of two new calcium silicate-based and epoxy resin-based endodontic sealers was evaluated. Thirty recently extracted human maxillary incisors were instrumented and divided into three groups according to the sealer used: (1) AH Plus; (2) iRoot SP; and (3) MTA Fillapex. Sealer penetration into dentinal tubules was measured using CLSM. Then, a universal testing machine was used to compare the push-out bond strengths of the sealers to the root canal dentin. AH Plus and MTA Fillapex exhibited significantly higher sealer penetration than iRoot SP did (p > .05). The push-out bond strengths of AH Plus and iRoot SP were higher than that of MTA Fillapex. We concluded that greater penetration of the sealer into the dentinal tubules was not associated with higher bond strength among the three sealers tested.     

Which is effective on bond strength of resin-based sealers: incorporation of powdered dentin to primer or adhesive?

The aim of this study was to investigate the effect of adding powdered dentin to primer or adhesive in a self-etch system on the shear bond strength of three resin-based sealers. Seventy-two premolars were sectioned buccolingually, and 144 root halves were divided into three groups according to the sealer used: epoxy resin-based sealer (AH Plus), methacrylate resin-based (RealSeal, Hybrid Root SEAL) n = 48. The surfaces were irrigated with 5% NaOCl, 17% EDTA, distilled water for 5 min. Four subgroups were created (n = 12): control group; Clearfil Liner Bond 2 V treated group; powdered dentin added to the primer of Clearfil Liner Bond 2 V (40 wt.%); and powdered dentin added to the adhesive of Clearfil Liner Bond 2 V (20 wt.%). Dentin powder was prepared. Three mm high buildups with a constant surface area of 3.45 mm² were created using the sealers and allowed to set (37 ºC, 100% humid, 72 h). The samples were tested to failure for shear bond strength (1 mm/min). The data were calculated (MPa) and analyzed using two-way ANOVA, one-way ANOVA, and Tukey HSD tests. Adhesive use decreased the bonding performance of AH Plus (p = 0.00). Mean bond strength of the other sealers was found similar to control. Primer or adhesive resin with powdered dentin did not increase the adhesive performance of the self-etch system used. The shear bond strength of RealSeal was significantly increased when powdered dentin was added to primer or adhesive (p = 0.00). The effect of adding powdered-dentine to primer or adhesive in a self-etch system on the shear bond strength was sealer-dependent .     

The effect of Nd:YAG laser irradiation on root surface: shear-bond strength and SEM–EDX study

To investigate the effects of Nd:YAG laser and 17% EDTA treatment on root-dentin mineral content using scanning electron microscope–energy dispersive X-ray spectroscopy (SEM–EDX) and on shear-bond strength of epoxy-resin-based sealer (AH Plus) to root dentin. Twelve extracted premolars were decoronated and roots were sectioned, so that 24 two-root halves were obtained. Element levels of each half were examined by SEM/EDX, and AH Plus build-ups were created. After shear-testing, the test surfaces were reground and subjected to a 5.25% NaOCl. Two subgroups were created according to the surface treatment (n = 12): G1, with 17% EDTA for 5 min; G2, with the Nd:YAG laser. The element level analysis and shear-bond strength test were repeated for each half; the data were recorded (MPa) and analyzed (paired samples t-test). The EDTA treatment increased the O, C, Ca/P ratio (p < 0.001), decreased Ca, P content (p = 0.000), but did not change Na, Mg content (p > 0.05). The Nd:YAG laser increased O, Ca/P ratio (p < 0.001), and decreased the Ca, P content (p = 0.000). The C, Mg, Na content did not change with the Nd:YAG laser (p > 0.05). Both 17% EDTA and Nd:YAG laser had an effect on the mineral content of roots. The 17% EDTA treatment decreased the shear-bond strength of AH Plus to root dentin (p = 0.000); however, the Nd:YAG laser did not affect the bond strength (p = 0.238). Thus, an Nd:YAG laser can be used for disinfection of the root canal when AH Plus is used as a sealer.     

Effect of calcium hydroxide removal techniques on the bond strength of root canal sealers

To evaluate the influence of calcium-hydroxide(CH) with different vehicles on the push-out bond strength of different canal sealers to radicular dentin. 152 decrowned single-rooted human teeth were used. After preparation of root canals with nickel-titanium rotary files, 8 roots served as control groups. Then, the roots were divided as follows: (1) Calasept and (2) Surepaste (n = 72). Roots were further subgrouped according to the CH removal techniques: (1) %17 ethylenediaminetetraacetic acid (EDTA) + rotary file, (2) %17EDTA + hand file, and (3) %17EDTA (n = 24). Eight roots from each group sectioned longitudinally, divided into two pairs and photographed by stereomicroscope (n = 16). The remaining 16 roots in CH intracanal dressing groups were further divided into 2 subgroups according to the sealer used: (1) AH-Plus-jet and (2) Apexit-Plus (n = 8). Bond strengths of the root canal sealers to root canal dentin were measured using a push-out test setup. The data were statistically analyzed using multivariate analysis of variance p = 0.05. The push-out bond strength values were significantly affected by type of vehicle and the removal techniques (p < 0.05). The mean bond strength of AH-Plus-jet was significantly higher than Apexit-Plus, regardless of type of vehicle and the removal techniques (p < 0.05). There was no difference between vehicles on CH removal (p > 0.05). When examining the removal techniques, only irrigation with %17 EDTA left significantly larger amount of residue (p < 0.05). AH-Plus-jet showed better dislocation resistance than Apexit-Plus. Type of vehicle does not play a fundamental role in the degree of persistence of CH residues on the dentin walls. Instrumentation improves the removal efficiency of CH from root canal.     

The effect of combined use of chitosan and PIPS on push-out bond strength of root canal filling materials

Adhesion of root canal filling materials to root dentin is important for the long-term success of the treatment. Push-out bond strength test is used to evaluate the adhesion capacity of root canal filling materials to root canal walls. The aim of the present study is to compare the bond strength of root canal filling materials to root dentin after irrigation with EDTA, chitosan and the combination of chitosan and PIPS irridation using push-out bond strength test. Forty-eight extracted teeth were resected until 13-mm long roots were obtained. Root canals were prepared with a size-25 OneShape instrument. Samples were divided into three groups each including 15 roots. Group 1: Canals were rinsed with 0.2% chitosan and subjected to laser irridation with PIPS at the same time. Group 2: Canals were rinsed with 0.2% chitosan. Group 3: Canals were rinsed with EDTA. All canals were filled with .06 tapered gutta-percha and AH-plus sealer. One-mm thick slices were taken from coronal, middle and apical one-thirds of the roots. Push-out bond strength was determined using a Universal Testing Machine. One root from each group was observed under SEM to evaluate the degree of smear removal. Statistical analysis was performed with Kruskall-Wallis test. Results showed that bond strength values were statistically similar in overall evaluation for all groups (p > .05). In segmental evaluation, group 1 revealed the highest bond strength in apical one-third compared to other groups (p < .05).     

Effect of root canal sealers on the push-out bond strengths of tooth-colored posts to root dentine

Objectives: This study evaluated the effect of different root canal sealers on the push-out bond strength of tooth-colored posts to root dentin. Material and methods: Eighty human mandibular premolar teeth with single roots were decoronated and randomly divided into two groups according to post material: G1–G5: Cytec blanco; G6–G10: Cosmopost. In each group, the specimens were further subgrouped according to the filling material plus sealer (n = 8): G1, G6: Gutta-percha + AH Plus; G2, G7: Resilon + Epiphany SE; G3, G8: Gutta-percha + Sealite; G4, G9: Gutta-percha + iRoot SP; and G5, G10: control (unobturated). Cytec blanco and Cosmopost of 1.4 mm diameter were adhesively luted to samples using Variolink II. Push-out test was performed in a universal testing machine, and failure modes were examined under stereomicroscope. Data were analyzed with the two-way ANOVA and post hoc Tukey’s tests. Statistical significance was set to 0.05. Results: Roots obturated with AH Plus (3.48 ± 1.41 MPa), Sealite (3.47 ± 0.65 MPa), and Resilon (3.36 ± 1.23 MPa) had the lowest bond strength (p < 0.005). iRoot SP and control group samples showed the highest bond strength values (7.38 ± 0.89 MPa and 6.43 ± 1.16 MPa, respectively) (p < 0.05). Significant differences were observed among tooth-colored posts and sealers (p < 0.05). Adhesive failures were predominant in all groups (48%). Conclusions: When the resin cement Variolink II was used, the types of root canal filling materials and sealers could affect the retentions of the fiber/zirconium posts; the fiber post revealed the higher bond values than the zirconium post; and the calcium silicate-based sealer (iRoot SP) revealed the highest bond strengths.     

Impact of conditioning regimens and time on adhesion of a fiber post to root dentin using two resin cements

The aim of this study was to evaluate the influence of irrigation protocols on the bond strength of a glass fiber post bonded to dentin using two resin cements. In 200 root-filled teeth, post space was prepared and divided into five groups (n?=?40) based on the irrigation protocol: group 1 (3% sodium hypochlorite), group 2 (3% sodium hypochlorite – 17% Ethylene diamine tetraacetic acid), group 3 (a mixture of sodium hypochlorite and etidronic acid), group 4 (sodium hypochlorite – QMix), and group 5 (distilled water). Samples were subdivided into two subgroups (n?= 10) and fiber posts were cemented using subgroup A (Self-adhesive dual-cure resin cement; SEA) or B (dual-cure resin cement following an etch-and-rinse protocol, ER). Push-out bond strength was performed after 24 h and four months (n?= 10) and failure modes were categorized. Statistical analysis of data was carried out by appropriate analyses (p < 0.05). The irrigation protocol and the resin cement had a significant impact on push-out bond strength. Subgroup A group showed lower bond strength than B at both time periods when 3% NaOCl–17% EDTA and 1:1 mixture of 6% NaOCl + 18%EA protocols were used. Three percent NaOCl used in combination with 17% EDTA or QMix significantly decreased the push-out bond strength of ER at the end of four months (p < 0.05). In conclusion, dual-cure resin cements bonded with etch-and-rinse protocol showed highest bond strength when a mixture of NaOCl and etidronic acid was used as root canal irrigant. These values were differentially influenced by time.     

Influence of the ER,CR:YSGG laser and different irrigation methods on push-out bond strength of fiber post

Er,Cr:YSGG lasers are currently being investigated for disinfecting the root canal treatment. The aim of this study was to compare the effects of various irrigation protocols on push-out bond strength of fiber posts. Fifty maxillary anterior teeth were divided into five groups (n = 10) according to the protocol that applied into the post space. Group-1: distilled water, Group-2: 5% NaOCl, Group-3: 2% CHX, Group-4: Er,Cr:YSGG laser (1.5 W, 20 Hz, 85 air, 75 water, 26.7 J/cm²), Group-5: Er,Cr:YSGG laser (1.25 W, 50 Hz, 34 air, 24 water, 12.7 J/cm²). Fiber posts were cemented with resin cement. The remaining part of the root, three slices were obtained from each specimen and push-out test was performed. One-way ANOVA and Duncan’s test at a 5% level of significance were used for the statistical analysis. Post space irradiation with Er,Cr:YSGG laser (1.5 W 20 Hz, 85 air, 75 water, 26.7 J/cm²) increases push-out bond strength of fiber post to root canal dentin. Further investigations are needed to establish and optimize ER,Cr:YSGG laser parameters to increase the push-out bond strength of fiber posts.     

Comparative evaluation of push-out bond strength of Neo MTA Plus with Biodentine and white ProRoot MTA

Objective: The aim of this in vitro study was to evaluate the bond strength of a new calcium silicate cement, Neo MTA Plus (Avalon Biomed Inc. Bradenton, FL, U.S.A) by comparing ProRoot MTA and Biodentine.

Material and Methods: Sixty dentin slices were instrumented to achieve a diameter of 1.3 mm. Group 1: white ProRoot MTA, group 2: Biodentine, group 3: Neo MTA Plus – G (powder mixed with gel), group 4: Neo MTA Plus – W (powder mixed with distilled water) were loaded into cavities. The push-out bond strength values were measured. Data were analyzed using Welch ANOVA with Bonferroni correction p = 0.05. Failure modes (adhesive, cohesive, and mixture) were analyzed.

Results: The highest bond strength value was recorded in Neo MTA Plus mixed with gel (5.23 ± 1.78 MPa), whereas white ProRoot MTA (2.57 ± 0.66 MPa) had the lowest. Bond strength values of Neo MTA Plus mixed either with gel or with distilled water were statistically different from both white ProRoot MTA and Biodentine (2.61 ± 0.70 MPa) (p < 0.05). Adhesive failure was predominantly observed in all groups.

Conclusion: Neo MTA Plus could be considered as alternatives to the ProRoot MTA and Biodentine due to its better performance in bonding to root dentin.     

Effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with Bioaggregate apical plugs

To evaluate the effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with DiaRoot BioAggregate (BA) when used as apical plugs. Forty single-rooted teeth were prepared using Peeso reamers. The samples were divided into four groups. The intracanal medicaments were applied to the root canals as follows: Group1: a combination of metronidazole–ciprofloxacin–cefaclor, Group2: a combination of metronidazole–ciprofloxacin, Group3: calcium hydroxide, and Group4: no medication. After 21 days, the medicaments were removed. The apical part of each root was horizontally sectioned into 1-mm thick slices. The samples were divided into two subgroups, and the following materials were placed: Biodentine, DiaRoot-BioAggregate. After 48-h incubation, the push-out bond strength was measured. The data were analyzed by a two-way ANOVA. Biodentine showed a significantly higher mean push-out bond strength value than DiaRoot-BioAggregate (P = 0.00). The medications have an effect on the push-out bond strength of both materials (P = 0.002). Biodentine showed better adhesive performance as an apical plug than DiaRoot-BioAggregate.     

Effects of mixing techniques and dentin moisture conditions on push-out bond strength of ProRoot MTA and Biodentine

Objective: To evaluate the influence of manual and mechanical mixing techniques as well as the effects of moisture on the push-out bond strength of ProRoot MTA (Dentsply Tulsa Dental, Tulsa, OK, USA) and Biodentine (Septodont, Saint Maur des Fosses, France) to radicular dentin.

Material and methods: Two hundred and forty dentin discs were assigned into three groups with respect to the moisture condition tested: (1) dry, (2) paper points, (3) wet. The discs were further divided into four subgroups according to the calcium silicate cements (CSCs) and mixing techniques used: (1) ProRoot MTA mixed manually, (2) ProRoot MTA mixed mechanically, (3) Biodentine mixed manually, and (4) Biodentine mixed mechanically. Bond strengths of the cements to root canal dentin were measured using a push-out test setup. The data were statistically analyzed using three-way ANOVA and Bonferroni post hoc test p = 0.05.

Results: The data indicated that the push-out bond strength values were significantly affected by CSCs, mixing techniques, and moisture conditions (p < 0.001). Dry conditions caused a significant decrease in bond strength values for both CSCs (p < 0.001). The mean bond strength of Biodentine was significantly higher than that of ProRoot MTA, regardless of the mixing techniques and moisture conditions (p < 0.001). Mechanical mixing favored bond strength values statistically compared to manual mixing (p < .001).

Conclusion: The mixing techniques and moisture conditions have an effect on the push-out bond strengths of ProRoot MTA and Biodentine. Dry samples and manual mixing of cements deteriorate the push-out bond strengths values.     

Long-term bond strength of fiber posts cement to dentin with self-adhesive or conventional resin cements

The aim of this study was to evaluate the immediate and the long-term push-out bond strength of glass fiber posts (GFP) cemented with conventional or self-adhesive dual-curing resin cements, at different root depths. Prior to cementation, the GFP (Reforpost #3, Angelus) were etched with 37% phosphoric acid for 30 s followed by silane for 1 min. Thirty canine roots were divided into two groups (n = 15) according to resin cement type: ARC – dual resin cement (RelyX ARC/3M ESPE) combined with an three-step etch-and-rinse adhesive (Adper Scotch Bond Multi-Purpose Plus 3M/ESPE) or U200 – self-adhesive resin cement (RelyX U200/3M ESPE). The manufacturer’s instructions were followed. After 48 h, the roots were cross-sectioned at three different depths, resulting in serial slices corresponding to the cervical, middle, and apical root thirds. Slices were randomly divided into two groups, according to the period of water storage prior to push-out bond strength analysis: 48 h or 180 days. The data (MPa) were analyzed using three-way ANOVA for randomized blocks (p < 0.05), which showed no significant interaction between the three factors (p = 0.716). The main study factors were also proven not significant (cement: p = 0.711; time: 0.288; root third: p = 0.646). In conclusion, root depth, cement type (self-adhesive or conventional), and storage in water for 180 days did not influence the bond strength of GFP to intracanal dentin.     

Effect of the frequency of mechanical pulses for fatigue aging testing on push-out bond strength between glass fiber posts and root dentin

The aim of this study was to evaluate the influence of the frequency of mechanical pulses during mechanical fatigue aging on bond strength between glass fiber posts and root dentin. Fiber posts were adhesively cemented in 30 bovine roots and the core was built up with composite. All specimens were subjected to mechanical cycling (45° angle; 37 °C; 50 N; 2×10⁶ pulses) at different frequencies: 2, 4, and 8 Hz. After the fatigue, each specimen was submitted to push out test. The bond strength was calculated for data analysis (one-way ANOVA, p = 0.05). The frequency did not affect the push-out (p = 0.7). The main failure mode was between dentin and cement in all groups. The mechanical pulses were not influenced by the bond strength between root dentin and fiber posts. Thus, it is possible to decrease the time-consuming in vitro tests involving posts without damaging the reliability of the test.     

The effect of root dentin conditioning protocols on the push-out bond strength of three calcium silicate sealers

Objectives: To compare the effects of irrigation protocols on the push-out bond strength of calcium silicate materials at two different time periods (7-days and 3-months). Materials and methods: Root canals (n=300) were irrigated with one of the following (n=60): group 1 (3% NaOCl–17% EDTA); group 2 (17% EDTA–3% NaOCl); group 3 (1:1 mixture of 6% NaOCl and 18% etidronic acid); group 4 (3% NaOCl–QMix 2in1); group 5 (3% NaOCl–2% chlorhexidine). Specimens were subdivided into three subgroups (n=20): A, Endosequence BC sealer [EBC]; B, MTA Plus [MTA-P]; C, Tech Biosealer Endo [TECH]. Specimens were suspended in phosphate buffered saline [PBS] for 7 days or 3 months (n=10 per sealer). Push-out bond strength was measured and data were analyzed (P=0.05). Results: MTA-P: showed the highest bond strength at both time periods, when NaOCl+EA was used as an irrigant. This was not significantly different from the strength produced when NaOCl–QMix was used for 7 days (P>0.05). There was no significant difference between the bond strengths of the three materials when irrigated with group 1, 2 or 5 (P>0.05), but these groups showed significantly lower bond strengths than groups 3 and 4 (P<0.05). While the bond strength of EBC and MTA-P in specimens irrigated with groups 3 and 4 improved significantly with time [P<0.05], this was not true for TECH. Conclusions: Push-out bond strength of calcium silicate cements was differentially influenced by irrigation protocol and time. MTA Plus showed the highest bond strength at both time periods, when root canals were irrigated with NaOCl+EA. The bond strength of Tech Biosealer Endo did not improve with time immaterial of the irrigation protocols.     

Effect of Er,Cr:YSGG laser surface conditioning on the adhesion of fiber- reinforced composite and zirconia intraradicular posts to the root dentin

This study evaluated the influence of Er,Cr:YSGG laser surface conditioning on push-out bond strength of different root posts to the root dentin. Extracted (N = 27, n = 9 per group) and endodontically treated human mandibular premolars were prepared to receive the posts. Three types of posts, namely quartz fiber (D), glass fiber (S), and zirconium dioxide post (C) were luted with resin cement. The posts were randomly assigned to one of the surface conditioning method: (a) No conditioning, control (L0), (b) Er,Cr:YSGG laser at 175 mJ, 3.5 W for 60 s (L1), and (c) at 225 mJ, 4.5 W for 60 s, with 60 μs pulse duration and repetition rate was 20 Hz (L2) irradiation. Six sections (two coronal, two middle, and two apical) were made in each tooth yielding to 1 mm thick specimens. The specimens were stored in distilled water at 37?°C for 24 h and push-out bond strength (MPa) was tested in a Universal Testing Machine (1 mm/min). Data were analyzed using Kruskall–Wallis and Dunns`s post hoc tests (α = 0.05). In group D, both laser treated groups (L1:16.16 ± 19.89; L2:8.24 ± 9.26) presented significantly less bond strength compared to control group (L0:28.3 ± 16.8) (p < 0.001). Mean push-out bond strength values did not significantly differ according to the root segments (coronal, middle, and apical) (p = 0.106). Application of Er,Cr:YSGG laser, with the parameters tested, did not increase the bond strength of zirconium glass fiber and zirconium oxide posts. Laser surface conditioning decreased the bond strength of quartz fiber posts in the root canal.     

Bonding ability of self-adhesive resin-cements after dentin biomodification with hyaluronic acid

Purpose: The present study evaluated the influence of the hyaluronic acid (HA) on the bonding ability of self-adhesive resin cements to dentin regarding the bond strength. Eighty bovine incisors were ground flat to obtain a 2-mm thick slices which received conical preparations. The specimens were randomly distributed into 4 groups (n = 15) according to the dentin pretreatment (1 – control: untreated dentin; 2 – application of HA) and the evaluation time (1 – control: immediate evaluation; 2 – hydrolytic degradation: 6 months of storage in water at 37 °C). Preparations received the application of a self-adhesive resin cement (RelyX U200 or MaxCem Elite). Push-out bond strength test was conducted (0.5 mm/min). The bond strength data was submitted to two-way ANOVA/Tukey’s test (α = 0.05). For U200, no significance was observed when comparing the immediate (24 h) and 6 months means for the control groups (unexposed specimens). Previous application of HA to dentin significantly reduced the bond strength of U200 to dentin in both evaluation times (p < 0.05). HA had no significant influence on the push-out bond strength means for the cement MAX in both evaluation times (p > 0.05). The type 1 failure mode (adhesive mode) occurred in 100% of the specimens, irrespective of the dentin treatment or evaluation times. Pretreatment of dentin with HA produces a material-dependent influence on the push-out bond strength. The bonding ability of RelyX U200 is negatively influenced by the pretreatment of dentin with HA, whereas the biomodification of dentin with this bioactive agent causes no impact for the cement MaxCem Elite.     

Degree of conversion and adhesion of methacrylate-based resin cements with phosphonic or phosphoric acid acrylate to glass fiber posts at different regions of intraradicular dentin

This study evaluated the degree of conversion (DC) and adhesion of methacrylate-based resin cements to glass fiber posts at different regions of intraradicular dentin. Single-rooted teeth (N?=?24, n?=?12 per group) were cut at the cement–enamel junction (CEJ), endodontically treated and post space (depth?=?8 mm) was prepared. Teeth were randomly divided into two groups according to the resin cements: (a) Group ML: methacrylate-based cement with phosphonic acid acrylate (Multilink Automix, Ivoclar Vivadent); (b) Group RXU: methacrylate-based cement with phosphoric acid acrylate (RelyX Unicem 2 Automix, 3 M ESPE). Fiber-reinforced composite root posts (RelyX Fiber Post, 3 M ESPE) were cemented according to the manufacturers’ instructions of the resin cements. Root slices of 2-mm thickness (n?=?3 per tooth) were cut below the CEJ 1, 3, and 5 mm apically. The DC of each section was analyzed with micro-Raman spectrometer and push-out test was performed in the Universal Testing Machine (0.5 mm/min). After debonding, all specimens were analyzed using optical microscope to categorize the failure modes. While data (MPa) were statistically evaluated using Kruskal Wallis, Mann–Whitney U tests for DC data 3-way ANOVA and Tukey’s tests were used (α?=?0.05). Regardless of the resin cement type, the mean push-out bond strength results (MPa), were significantly higher for the coronal slices (ML: 9.1?±?2.7; RXU: 7.3?±?4.1) than those of the most apical ones (ML: 7?±?4.9; RXU: 2.89?±?1.5) (p?=?0.002). Resin cement type and (p?p?=?0.002) significantly affected the DC values, while the interaction terms were not significant (p?=?0.606). Overall, DC was significantly higher for ML (67?±?8.2%) than RXU (26?±?8.8%) (p?相似文献   

Effect of caries removal techniques on bond strength to caries affected dentin on gingival wall: AFM observation of dentinal surface

Objective: The purpose of this study was to evaluate the effect of different caries removal techniques on the bond strength of two adhesive systems. Materials and Methods: Seventy human molar teeth that had proximal carious lesions were randomly divided into four groups according to the technique used to remove the caries: a conventional steel bur, an Er:YAG laser, a chemomechanical Carisolv^® gel, and air polishing. The groups were then divided into two subgroups according to the adhesives used: Clearfil S³ (Kuraray Co., Ltd, Tokyo, Japan) and Adper SE Plus (3 M ESPE, St Paul, MN, USA). The enamel and superficial dentin of the crown was flattened and caries were removed using different methods. Teeth were restored with composite and three 1 mm² stick-shaped microtensile specimens were prepared from each tooth. For each removal technique, the surface roughness of one dentin sample was analyzed using atomic force microscopy (AFM). The microtensile bond strength data were then analyzed using a two-way ANOVA, Kruskal–Wallis and Mann–Whitney U tests, and multiple comparison tests (p = 0.05). Results: The two-way ANOVA showed that the effects of the cavity preparation techniques and adhesive systems and their interaction were statistically significant (p < 0.05). The μTBS values were highest when bonding with Adper SE Plus to cavities prepared with the steel bur (37.20 ± 11.65 MPa) and lowest when bonding with Clearfil S³ Bond to cavities prepared with the Er:YAG laser (16.74 ± 6.95 MPa). Conclusion: The caries removal techniques affected the bond strength values of the self-etch adhesive systems.     

Endodontic irrigants effect on long-term intraradicular adhesion of resin cements

The aim of this study was to evaluate over time the bond strength of dual-cure and self-adhesive resin cements used for bonding fiberglass posts following irrigation with different solutions. Ninety roots from single-rooted premolars were selected and divided into 6 groups (n = 15) according to the resin cement, dual-cure or self-adhesive (RelyX ARC and RelyX U100) or the endodontic irrigant used (2% chlorhexidine digluconate - CH, 1% sodium hypochlorite - SH and deionized distilled water – control). Following post cementation, the roots were cross-sectioned in order to obtain two slices from each root third (cervical, mid and apical). The specimens were stored for 7 or 180 days in water and the push-out bond strength test applied. The data was analyzed using three-way ANOVA and Tukey Kramer. The interaction endodontic irrigants-resin cement vs. storage time was significant (p = 0.008), where 7 days of storage induced no difference between the groups, however, after 180 days, the groups for which CH or SH combined with RelyX U100 were used showed higher bond strength values than RelyX ARC, regardless of the irrigant solution. There was no difference between the use of RelyX ARC after 7 and 180 days of storage. For Rely X U100 180 days of storage increased the push-out bond strength when either CH or SH was used. The dual-cure and self-adhesive resin cements associated with CH or SH demonstrated similar immediate bond strength performance. The self-adhesive cement, however, showed improved bond strength over time when either irrigant was used.     

Bond strength of fiber posts and short fiber-reinforced composite to root canal dentin following cyclic loading*

The aim of this study was to evaluate the effect of cyclic loading on the bond strength of fiber posts and short fiber-reinforced composite (FRC) to root canal. One hundred single-rooted teeth were divided into two groups according to the material used for luting fiber posts: (1) Resin-core material (Gradia Core, GC Corp.) and (2) Short FRC (EverX Posterior, GC Corp.). Then the specimens were randomly assigned into three sub-groups according to the post material and the groups are indicated as follows: (1) Short FRC (EverX Posterior) used instead of post and core, (2) Fiber post (GC post, GC Corp.) cemented with resin-core (Gradia Core), (3) Fiber post (GC post, GC Corp.) cemented with short FRC (EverX Posterior), (4) Experimental fiber post cemented with resin-core (Gradia Core, GC Corp), (5) Experimental fiber post cemented with short FRC (EverX Posterior). Then the specimens were subdivided into a further two groups in accordance with the storage condition (cyclic loading and 24 h water storage-control group) (n = 10/per group). The micropush-out bond strength between root dentin and posts was measured. Data were analyzed using three-way ANOVA and Tukey HSD tests (α = 0.05). Micropush-out bond strength of the posts to dentin was significantly affected by the type of post material (p < 0.05). However, the load cycling and the resin-based luting agent used had no effect on bond strength values (p = 0.706 and p = 0.346, respectively).