A super-oscillatory lens optical microscope for subwavelength imaging

The past decade has seen an intensive effort to achieve optical imaging resolution beyond the diffraction limit. Apart from the Pendry-Veselago negative index superlens, implementation of which in optics faces challenges of losses and as yet unattainable fabrication finesse, other super-resolution approaches necessitate the lens either to be in the near proximity of the object or manufactured on it, or work only for a narrow class of samples, such as intensely luminescent or sparse objects. Here we report a new super-resolution microscope for optical imaging that beats the diffraction limit of conventional instruments and the recently demonstrated near-field optical superlens and hyperlens. This non-invasive subwavelength imaging paradigm uses a binary amplitude mask for direct focusing of laser light into a subwavelength spot in the post-evanescent field by precisely tailoring the interference of a large number of beams diffracted from a nanostructured mask. The new technology, which--in principle--has no physical limits on resolution, could be universally used for imaging at any wavelength and does not depend on the luminescence of the object, which can be tens of micrometres away from the mask. It has been implemented as a straightforward modification of a conventional microscope showing resolution better than λ/6.     

Resolution limit for two-dimensional crossed-grating patterns projected by a coherent beam

The resolution limit for two-dimensional crossed-grating patterns created by projecting mask objects by using a coherent beam has been investigated. We consider first two conventional mask types, a binary-amplitude mask and a two-level phase-shifting mask, in analyzing relationships between a diffraction-beam configuration and an image-intensity distribution. Then we derive, as a mask that overcomes the resolution limit of the conventional ones, a four-level phase-shifting structure with which the minimum image period can be reduced to square root(2)/2 time that of the two-level phase-shifting mask.     

Super high resolution for long-range imaging

A new optical system with a resolution that is superior to the resolution of the usual optical systems with diffraction limit is presented. We introduce a newly generated narrow light beam that propagates for a long range while almost maintaining its beam width and show that the beam width is narrower than that of the diffraction limit of normal optics. Thus a super high resolution is achieved for a long range, e.g., a range of a few kilometers, by the use of a 10-cm-diameter telescope. The high resolution for long-range imaging can be obtained by a Galilean telescope with a negative eyepiece that has a spherical aberration. We demonstrate theoretically high-resolution imaging by using simple objects and assuming a telescope 10 cm in diameter and a visible wavelength. A comparison of simulation results by the conventional optical system and by the special optical system clearly shows the superiority of the new system.     

High-resolution neutron microtomography with noiseless neutron counting detector

The improved collimation and intensity of thermal and cold neutron beamlines combined with recent advances in neutron imaging devices enable high-resolution neutron radiography and microtomography, which can provide information on the internal structure of objects not achievable with conventional X-ray imaging techniques. Neutron detection efficiency, spatial and temporal resolution (important for the studies of dynamic processes) and low background count rate are among the crucial parameters defining the quality of radiographic images and tomographic reconstructions. The unique capabilities of neutron counting detectors with neutron-sensitive microchannel plates (MCPs) and with Timepix CMOS readouts providing high neutron detection efficiency (∼70% for cold neutrons), spatial resolutions ranging from 15 to 55 μm and a temporal resolution of ∼1 μs—combined with the virtual absence of readout noise—make these devices very attractive for high-resolution microtomography. In this paper we demonstrate the capabilities of an MCP-Timepix detection system applied to microtomographic imaging, performed at the ICON cold neutron facility of the Paul Scherrer Institute. The high resolution and the absence of readout noise enable accurate reconstruction of texture in a relatively opaque wood sample, differentiation of internal tissues of a fly and imaging of individual ∼400 μm grains in an organic powder encapsulated in a ∼700 μm thick metal casing.     

Raman and luminescence probes for the study of compound semiconductors

Optical characterization using local probes with submicrometric spatial resolution is very useful for many problems concerning compound semiconductors and devices. In particular, micro-Raman spectroscopy and cathodoluminescence spectrum imaging are very powerful analytical techniques that manage good signal/noise ratios allowing to acquire images including spectral information and slightly submicrometric spatial resolution in a short time. The main analytical and spatial resolution aspects concerning both techniques are addressed. Several examples are presented in order to illustrate the capabilities of micro-Raman and cathodoluminescence spectrum imaging for the characterization of compound semiconductors and the devices based on them.     

Flexible depth-of-field imaging system using a spatial light modulator

A major problem of optical microscopes is their small depth-of-field (DOF), which hinders automation of micro object manipulation using visual feedback. Wavefront coding, a well-known method for extending DOF, is not suitable for direct application to micro object manipulation systems based on visual feedback owing to its expensive computational cost and due to a trade-off between the DOF and the image resolution properties. To solve such inherent problems, a flexible DOF imaging system using a spatial light modulator in the pupil plane is proposed. Especially, the trade-off relationship is quantitatively analyzed by experiments. Experimental results show that, for low criterion resolution, the DOF increases as the strength of the mask increases, while such a trend was not found for high criterion resolution. With high criterion resolution, the DOF decreases as the mask strength increases when high-resolution images are required. The results obtained can be used effectively to find the optimum mask strength given the desired image resolution.     

Superresolution with nonorthogonal polarization coding

One can achieve high-resolution (superresolution) imaging, beyond the classical limit, by exploiting certain degrees of freedom such as time and polarization for the object under consideration. We present an implementation, based on polarization coding, that requires insertion of a single mask into the object plane followed by postprocessing of the detected signal. We describe the procedure and provide experimental evidence for the implementation of the proposed technique.     

Apertureless near-field/far-field CW two-photon microscope for biological and material imaging and spectroscopic applications

We present the development of a versatile spectroscopic imaging tool to allow for imaging with single-molecule sensitivity and high spatial resolution. The microscope allows for near-field and subdiffraction-limited far-field imaging by integrating a shear-force microscope on top of a custom inverted microscope design. The instrument has the ability to image in ambient conditions with optical resolutions on the order of tens of nanometers in the near field. A single low-cost computer controls the microscope with a field programmable gate array data acquisition card. High spatial resolution imaging is achieved with an inexpensive CW multiphoton excitation source, using an apertureless probe and simplified optical pathways. The high-resolution, combined with high collection efficiency and single-molecule sensitive optical capabilities of the microscope, are demonstrated with a low-cost CW laser source as well as a mode-locked laser source.     

密集式MIMO声呐高分辨成像实验研究

为验证密集式多输入多输出(Multi-Input Multi-Output,MIMO)声呐高分辨成像算法的有效性,进行了水池实验并获得了期望的高分辨成像结果。首先,将成像算法分为角度维高分辨成像和距离维高分辨成像两种,分别建立了各自的成像处理流程。角度维高分辨成像基于虚拟阵列波束形成算法,距离维高分辨成像基于大带宽信号合成算法。然后,根据两种处理流程选择合适的发射信号和阵型,并据此搭建密集式MIMO声呐高分辨成像平台进行水池实验。最后,通过与传统单输入多输出(Single-Input Multi-Output,SIMO)声呐的成像结果进行对比,证明了MIMO声呐可结合发射信号、阵型和相应的处理流程获得更高的角度分辨率和距离分辨率。     

High-resolution scanning phase retrieval with a probe beam of unknown modulus

A high-resolution phase-retrieval method for an imaging system with scanning illumination that is capable of reconstructing the modulus and phase of an object without a holographic reference wave is proposed. Reconstruction involves the synthesis of the reconstructed objects from the data of zero- and higher-frequency components of two Fourier intensity measurements: the Fourier intensity of the product of the object and a probe beam that is scanned across the object and the Fourier intensity of the product of the object and a probe beam that is modulated with an exponential filter. This method improves on the resolution of a reconstructed object by previous methods that make use of the data of only the zero-frequency components of the two Fourier intensities. In addition, phase retrieval in the scanning-illumination system with a probe of unknown modulus can be treated by use of the synthetic procedure, provided the phase of the probe function is a constant or a known distribution. Computer-simulated examples of the reconstruction of one- and two-dimensional complex objects demonstrate that reconstruction is robust.     

High-resolution imaging in a deep turbid medium based on an ultrasound-switchable fluorescence technique

The spatial resolution of fluorescence imaging techniques in deep optically turbid media such as tissues is limited by photon diffusion. To break the diffusion limit and achieve high-resolution and deep-tissue fluorescence imaging, a fundamentally different method was demonstrated based on a concept of ultrasound-switchable fluorescence. The results showed that a small fluorescent tube with a diameter of ～180?μm at a depth of ～20?mm in an optical scattering medium ([Formula: see text] and [Formula: see text] cm(-1)) can be clearly imaged with a size of ～260?μm. The depth-to-resolution ratio is shown to be about one order of magnitude better than other deep-tissue fluorescence imaging techniques.     

Description and performance of a prototype PET system for small volume imaging

A prototype positron emission tomography (PET) system has been designed for high-resolution imaging of small volumes. The detectors use Pb converter stacks and multiwire proportional counters (MWPC); the data acquisition components and image reconstruction methods are also described briefly. The performance of the system is discussed in terms of sensitivity, count rate capability, spatial resolution, and scattered background. Three examples of metabolic or transport imaging demonstrate the capabilities and limitations of the system. These are blood flow to bone, cerebral glucose uptake, and nutrient translocation in plants. The performance of the prototype has been sufficiently promising that an improved system is under development.     

Simultaneous measurement of diameter and position of spherical particles in a spray by an original imaging method

The size and the position of individual particles in a falling spray crossing a horizontal plane are measured simultaneously with a shadowgraph imaging setup. An original method for measurement of drop sizes based on a prescribed analytical form of the luminance distribution of the objects (i.e., the drops) is presented. The processing time is strongly reduced compared with that for the usual inversion techniques. The turbulent dispersion of drops in a grid-generated turbulence is studied. Evidence for the presence of coalescence in the spray is presented.     

Determination of the size and structure of an X-pinch x-ray source from the diffraction pattern produced by microfabricated slits

X-pinch plasma emits subnanosecond bursts of x rays in the 3-10-keV energy range from a small source. As such, it has been used for high-resolution point-projection imaging of small, dense, rapidly changing plasmas as well as for submillimeter-thick biological samples. In addition to the effect of source size on geometric resolution, a small source size can also provide high spatial coherence of x rays, enabling the rays to be used for imaging weakly absorbing objects with excellent spatial resolution by a method called phase-contrast imaging. To determine the source size, we microfabricated gold slits and imaged them in a point-projection radiography configuration. The shape of the shadow image pattern depends on the source size and energy band of the x rays, the shape and material used for the slits, and the geometry of the experiment. Experimental results have been compared with wave-optics calculations of the expected image pattern as a function of all the parameters listed above. For example, assuming a Gaussiansource distribution, an effective source size in 2.5-4.1 A radiation (1 A = 0.1 nm) of 1.2 +/- 0.5 microm (full width at half-maximum) was determined for a 20-microm Mo wire X pinch. Characterization of the size and structure of the x-ray bursts from X pinches by the use of different wire materials and different slit structures is made.     

Subwavelength surface-relief gratings for stellar coronagraphy

We present a new design of a phase mask coronagraph implemented with subwavelength diffractive optical elements consisting of optimized surface-relief gratings. Phase mask coronagraphy is a recent technique that seeks to accommodate both high dynamic and high angular resolution imaging of faint sources around bright astrophysical objects such as exoplanets orbiting their host stars. The original design we propose is a new, integrated, and flexible solution to the pi phase-shift chromaticity of the phase mask coronagraphs. It will allow broadband observations, i.e., shorter integration times and object characterizations, by means of spectroscopic analysis. The feasibility of the component manufacturing is also considered through a tolerance study.     

相移掩模方法及其一维数值模拟

相移掩模方法是一种新的光刻技术，它可以提高现有光刻设备的分辨率，使超大规模集成电路及二元光学的制作迈上一个新台阶。本文介绍了相移掩模方法的基本原理，用部分相干光成象理论分析了用于光刻的投影照相系统的成象特性，导出了一维成象的简化公式，对一维光栅结构进行了计算机数值模拟并给出了模拟结果。     

Quantitative structured-illumination phase microscopy

We introduce a quantitative phase imaging method for homogeneous objects with a bright field transmission microscope by using an amplitude mask and a digital processing algorithm. A known amplitude pattern is imaged on the sample plane containing a thick phase object by placing an amplitude mask in the field diaphragm of the microscope. The phase object distorts the amplitude pattern according to its optical path length (OPL) profile, and the distorted pattern is recorded in a CCD detector. A digital processing algorithm then estimates the object's quantitative OPL profile based on a closed form analytical solution, which is derived using a ray optics model for objects with small OPL gradients.     

The influence of disturbing effects on the performance of a wide field coded mask X-ray camera

The coded aperture telescope, or Dicke camera, is seen as an instrument suitable for many applications in X-ray and gamma ray imaging. In this paper the effects of a partially obscuring window mask support or collimator, a detector with limited spatial resolution, and motion of the camera during image integration are considered using a computer simulation of the performance of such a camera. Cross correlation and the Wiener filter are used to deconvolve the data. It is shown that while these effects cause a degradation in performance this is in no case catastrophic. Deterioration of the image is shown to be greatest where strong sources are present in the field of view and is quite small ( 10%) when diffuse background is the major element. A comparison between the cyclic mask camera and the single mask camera is made under various conditions and it is shown the single mask camera has a moderate advantage particularly when imaging a wide field of view.     

Shannon number and information capacity of three-dimensional integral imaging

Integral imaging systems performance has been previously investigated with regard to different parameters such as lateral resolution, field of view, and depth of view. Those parameters are linked to one another, and, since the information capacity of an integral imaging system is finite, there are always trade-offs among them. We use the Shannon number and information capacity limit as figures of merit of integral imaging systems. The Shannon number and information capacity provide compact assessments of the system and are useful for analysis and design. The limitations on the Shannon number and the information capacity of an integral imaging system are determined by the recording and display media.