沪酿3.042米曲霉富硒条件的探索

目的：探索沪酿3．042米曲霉的富硒能力并找到最佳的富硒条件。方法：于豆芽汁液体培养基中添加一系列浓度的亚硒酸钠，培养一段时间，研究亚硒酸钠浓度对米曲霉生物量、富硒能力，蛋白酶活力的影响，找到适合的亚硒酸钠浓度，测定此浓度下米曲霉的生长曲线、富硒量曲线，找到最佳的富硒条件，分析此条件下富硒米曲霉胞内硒的存在状态。结果：亚硒酸钠浓度为100mg／L时米曲霉的生物量最大，富硒能力最高，50mL液体培养基可以收获0．454g湿茵体，含有胞内硒2mg，其中有机硒含量达到95％以上，最适合的茵体收获时间是48h。但是此时蛋白酶活力降低明显，亚硒酸钠浓度为50mg／L时蛋白酶活力比不加亚硒酸钠时活力更高，36h达到活力峰，此时富硒能力也较强。结论：米曲霉是个很好的富硒载体，亚硒酸钠浓度为100mg／L时富硒能力最高，应用前景很好。     

富硒乳酸菌的筛选及鉴定

对5 种15 株乳酸菌的生长曲线进行了测定,并对亚硒酸钠添加质量浓度、耐硒和富硒能力进行比较,筛选出了富硒优势乳酸菌,并进行了生理生化和分子鉴定。结果表明：适宜的加硒时间为培养后的第6小时,培养时间为24 h。通过对9 株菌株比较,确定了较适宜的亚硒酸钠添加质量浓度为6 μg/mL。最终筛选出BC-25为富硒优势菌株,富硒量425.79 μg/g,硒转化率达27.00%,经16S rDNA分析鉴定其为植物乳杆菌（Lactobacillus plantaru）。     

大麦苗富硒研究

目的：探讨大麦苗不同富集硒的方法以及不同浓度的亚硒酸钠对大麦苗的生长及富硒量的影响。方法：采用水培法栽培大麦苗;富集硒的方法分别为浸麦富硒法、出苗后富硒法以及全程富硒法;透析袋法分离有机与无机硒;原子荧光光谱法测定富硒大麦苗中的硒含量。结果：浸麦富硒时,当亚硒酸钠浓度低于50mg／L时大麦苗生长旺盛,麦苗出率与生长曲线均高于空白对照组;出苗后富硒,亚硒酸钠为30mg／L时,其生长曲线高于空白对照组,其富硒量最高;全程富硒时,大麦苗出芽率,麦苗出率低,且生长迟缓。结论：出苗后富硒,当亚硒酸钠浓度为30mg／L时,大麦苗生长快,且富硒量最高,全硒含量是空白对照组的4．97倍,有机硒含量是空白对照的8．05倍。此为本实验中最佳富硒方法与最佳富硒浓度。     

假丝酵母发酵丢糟生产富硒饲料蛋白的研究

将假丝酵母接种在添加亚硒酸钠的培养液中进行驯化,以大曲丢糟为主要原料,以富硒假丝酵母为菌种进行富硒饲料蛋白的发酵生产,通过正交试验得到最佳的饲料生产工艺条件为：亚硒酸钠添加量为0．75mg／kg,富硒假丝酵母接种量为15％,发酵时间为4d。该富硒饲料粗蛋白和有机硒含量分别达到了17．8％、0．17mg／kg。     

曲酒丢糟培养A地霉生产富硒饲料蛋白的研究

将白地霉接种在添加亚硒酸钠的培养液中进行驯化，以大曲丢糟为主要原料，以富硒白地霉为菌种，适当添加纤维素酶进行富硒饲料蛋白的发酵生产，通过正交试验得到最佳的饲料生产工艺条件为：Na2SeO3添加量为0．50mg／kg，富硒白地霉接种量为15％，纤维素酶添加量为0．03％，培养时间4d。所生产得的富硒饲料粗蛋白和有机硒含量分别达到了18．9％、0．18mg＆g。     

啤酒酵母的富硒条件和效果研究

研究了硒添加量、接种量、培养时间及装液量对啤酒酵母富硒作用的影响。结果表明，培养基中的硒添加量是影响啤酒酵母富硒效果的最主要因素，在初步确定的优化培养条件下（硒添加量20mg／L，接种量5％，培养时间20h，装液量60mL／250mL三角瓶），富硒酵母中的硒含量达920．3μg／g，硒总含量达4038．7μg／L，富硒效果较好。     

富硒乳酸菌种的筛选

本实验从样品中分离，纯化，筛选出嗜酸乳杆菌(Lactobacillus acidophilus，简称LA)，保加利亚乳杆菌(Lactobacillus Bulgaria,简称LB)，嗜热链球菌(Streptococcus thermopiles，简称ST)。通过富硒抗性筛选，确定了LA为耐硒菌种。通过对其发酵培养获得富硒能力优良的菌株和富硒条件，试验结果表明：选用番茄汁液体培养基，接种量10％，加硒量18ug／ml，pH6．8，37℃培养24h，有机硒含量可达12．99μg／ml，转化率为72．17％。     

富硒乳酸菌筛选及其富硒工艺初探

首先对含有嗜酸乳杆菌(La),保加利亚乳杆菌(Lb),嗜热链球菌(St)的分离样品进行分离,纯化,筛选出纯菌种,然后分别进行富硒试验,通过抗性筛选和三角瓶发酵培养获得富硒能力优良的菌株和富硒条件。试验结果表明,嗜酸乳杆菌(La)富硒能力最强,其富硒条件初步确定为:选用番茄汁液体培养基,亚硒酸钠添加量为18μg/mL,pH6.6,37℃,培养24h,富硒能力为62.22%。      

富硒乳酸菌筛选及其富硒工艺初探

首先对含有嗜酸乳杆菌(La),保加利亚乳杆菌(Lb),嗜热链球菌(St)的分离样品进行分离,纯化,筛选出纯菌种,然后分别进行富硒试验,通过抗性筛选和三角瓶发酵培养获得富硒能力优良的菌株和富硒条件。试验结果表明,嗜酸乳杆菌(La)富硒能力最强,其富硒条件初步确定为:选用番茄汁液体培养基,亚硒酸钠添加量为18μg/mL,pH6.6,37℃,培养24h,富硒能力为62.22%。     

富硒益生菌的筛选及其富硒条件的优化

为了获得能耐受较高亚硒酸钠质量浓度和具有富硒能力的益生菌菌株,对7株酵母菌和12株乳酸菌进行了筛选。结果表明,所有菌株均能在亚硒酸钠质量浓度为20~80μg/m L的平板上生长,乳酸菌YQRS菌株和酵母菌FJYJM3菌株具有较高的耐受性和富硒能力。对它们进行发酵条件的优化,表明YQRS菌株在亚硒酸钠添加量为15μg/m L、添加硒的时间为对数期前期时,富硒效果最好,菌体生物量为2.66 g/L,总硒含量能达到2 300.26μg/L。而FJYJM3菌株在亚硒酸钠添加量为20μg/m L,添加硒的时间为对数期前期时,富硒效果最显著,生物量能达到4.86 g/L,总硒含量能达到5 790.99μg/L。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press