Performance of dairy cows fed annual ryegrass silage and corn silage with steam-flaked or ground corn

Twenty-four lactating Holstein cows were used in a 6-wk randomized block design trial with a 2 × 2 factorial arrangement of treatments to determine the effects of feeding ground corn (GC) or steam-flaked corn (SFC) in diets based on either annual ryegrass silage (RS) or a 50:50 blend of annual ryegrass and corn silages (BLEND). Experimental diets contained 49.6% forage and were fed as a total mixed ration once daily for 4 wk after a 2-wk preliminary period. No interactions were observed among treatments. Cows fed BLEND consumed more dry matter (DM), organic matter (OM), neutral detergent fiber (NDF), and acid detergent fiber (ADF) than those fed RS, but total-tract digestibility of OM, NDF, and ADF was greater for RS than for BLEND. No differences in nutrient intake were observed among treatments during wk 4 when nutrient digestibility was measured, but digestibility of DM and OM was greater for SFC than for GC. Cows fed BLEND tended to produce more energy-corrected milk than those fed RS, resulting in improved efficiency (kg of milk per kg of DM intake). When diets were supplemented with SFC, cows consumed less DM and produced more milk that tended to have lower milk fat percentage. Yield of milk protein and efficiency was greatest with SFC compared with GC. Blood glucose and milk urea nitrogen concentrations were similar among treatments, but blood urea nitrogen was greater for cows fed GC compared with those fed SFC. Results of this trial indicate that feeding a blend of annual ryegrass and corn silage is more desirable than feeding diets based on RS as the sole forage. Supplementing diets with SFC improved performance and efficiency compared with GC across forage sources.     

Ryegrass or alfalfa silage as the dietary forage for lactating dairy cows

Renewed interest exists in using grass forages to dilute the higher crude protein (CP) and lower digestible fiber present in legumes fed to lactating dairy cows. A 3 x 3 Latin square feeding study with 4-wk periods was conducted with 24 Holstein cows to compare ryegrass silage, either untreated control or macerated (intensively conditioned) before ensiling, with alfalfa silage as the sole dietary forage. Ryegrass silages averaged [dry matter (DM) basis] 18.4% CP, 50% neutral detergent fiber (NDF), and 10% indigestible acid detergent fiber (ADF) (control) and 16.6% CP, 51% NDF, and 12% indigestible ADF (macerated). Alfalfa silage was higher in CP (21.6%) and lower in NDF (44%) but higher in indigestible ADF (26%). A lower proportion of the total N in macerated ryegrass silage was present as nonprotein N than in control ryegrass and alfalfa silages. Diets were formulated to contain 41% DM from either rye-grass silage, or 51% DM from alfalfa silage, plus high moisture corn, and protein concentrates. Diets averaged 17.5% CP and 28 to 29% NDF. The shortfall in CP on ryegrass was made up by feeding 7.6% more soybean meal. Intake and milk yields were similar on control and macerated ryegrass; however, DM intake was 8.3 kg/d greater on the alfalfa diet. Moreover, feeding the alfalfa diet increased BW gain (0.48 kg/d) and yield of milk (6.1 kg/d), FCM (6.8 kg/d), fat (0.26 kg/d), protein (0.25 kg/d), lactose (0.35 kg/d), and SNF (0.65 kg/d) versus the mean of the two ryegrass diets. Both DM efficiency (milk/DM intake) and N efficiency (milk-N/N-intake) were 27% greater, and apparent digestibility was 16% greater for DM and 53% greater for NDF and ADF, on the ryegrass diets. However, apparent digestibility of digestible ADF was greater on alfalfa (96%) than on ryegrass (average = 91%). Also, dietary energy content (estimated as net energy of lactation required for maintenance, milk yield, and weight gain) per unit of digested DM was similar for all three diets. Results of this trial indicated that, relative to ryegrass silage, feeding alfalfa silage stimulated much greater feed intake, which supported greater milk production.     

Effect of length of cut and kernel processing on use of corn silage by lactating dairy cows

Forty Holstein cows were used in an 8-wk randomized block design trial to determine the effects of theoretical length of cut (TLC) and kernel processing (KP) of whole plant corn silage on nutrient intake and digestibility, milk yield, and milk composition. Corn was harvested at three-quarters milk line stage of maturity at TLC of 1.90 or 2.54 cm. At each TLC, corn was KP at either 2 or 8 mm roll clearance. The control was harvested at 1.90 cm without KP. Corn silage provided 38% of the dietary dry matter (DM) in the experimental diets. Intake of DM and nutrients was similar among treatments. Apparent digestibility of DM and acid detergent fiber (ADF) increased with increasing TLC. Fiber digestibility was improved by KP compared with unprocessed corn silage. Starch digestibility was greater for corn silage KP at 2 vs. 8 mm. Apparent digestibility of DM, crude protein, and ADF was lowest for the diet containing silage harvested at 2.54 cm TLC and KP at 8 mm, resulting in an interaction of TLC and KP. No differences were observed in DM intake (DMI) among treatments. An interaction of TLC and KP was observed, however, for yield of milk protein and energy-corrected milk (ECM) and efficiency of converting DMI to ECM because of lower yield for diets containing silage harvested at 2.54 cm TLC and KP at 8 mm. Results of this trial indicate that as TLC increases, aggressive KP is necessary to maintain nutrient digestibility and performance of lactating dairy cows.     

Effects of replacing grass silage with barley silage in dairy cow diets

This study examined the effects of gradually replacing grass silage with whole-crop barley silage on feed intake, ruminal and total tract digestibility, and milk yield in lactating dairy cows. Four dairy cows in early lactation, equipped with rumen cannulas, were fed 4 diets over four 21-d periods. The diets consisted of 4 forage mixtures of grass silage and whole-crop barley silage supplemented with 8.9 kg/d of concentrates [dry matter (DM) basis]. The proportion of barley silage in the forage was adjusted to 0, 0.20, 0.40, and 0.60 kg/kg of DM. Ruminal nutrient metabolism was measured on the basis of digesta flow entering the omasal canal. Ammonia concentrations and volatile fatty acid profiles were determined in the rumen fluid. Ruminal digestion and passage kinetics were assessed by the rumen evacuation technique. Replacement of grass silage with barley silage had no effect on DM, digestible organic matter, or neutral detergent fiber (NDF) intake, but starch intake increased, whereas nitrogen and digestible NDF (dNDF) intake decreased. Increases in the proportion of barley silage linearly decreased milk yield, and the molar proportion of acetate in the rumen, and increased that of propionate, butyrate, and valerate. Decreases in milk yield due to inclusion of barley silage were attributed to decreases in diet digestibility and nutrient supply to the animal. Barley silage linearly decreased organic matter digestibility in the total tract and NDF and dNDF digestibility in the rumen and the total tract, and decreased nonammonia N flow entering the omasal canal. No significant differences between diets were noted in the digestion rate of dNDF or passage rate of indigestible NDF from the rumen. Decreases in organic matter and NDF digestibility were attributed to the higher indigestible NDF concentration of barley silage compared with that of grass silage and to the smaller pool size of dNDF in the rumen.     

Effects of corn silage hybrid and dietary concentration of forage NDF on digestibility and performance by dairy cows

Eight intact multiparous cows and four ruminally and duodenally cannulated primiparous cows were fed four diets in a replicated 4 x 4 Latin square design: 1) 17% forage neutral detergent fiber (NDF) with brown midrib corn silage (BMRCS), 2) 21% forage NDF with BMRCS, 3) 17% forage NDF with conventional corn silage (CCS), and 4) 21% forage NDF with CCS. Diets contained 17.4% crude protein and 38.5% NDF. Each period consisted of 4 wk for intact cows and 2 wk for cannulated cows. For intact cows, DM intake was higher for BMRCS than CCS, and milk urea N was higher for 21 than 17% forage NDF. Milk protein yield tended to be higher and milk urea N lower for cows fed BMRCS than those fed CCS. Milk yield and milk protein percentage were similar among treatments. For the cannulated cows, ruminal mat consistency was similar among treatments. Based on a 72 h in situ incubation, BMRCS was lower in indigestible NDF than CCS. The BMRCS resulted in a higher proportion of ruminal propionate than CCS. Cows fed 21% forage NDF had a higher proportion of acetate and a lower proportion of propionate than cows fed 17% forage NDF. The total tract digestibility of nutrients and efficiency of bacterial N synthesis were similar among treatments, except that BMRCS resulted in lower intestinal fatty acid digestibility than CCS, and 17% forage NDF tended to result in higher total tract fatty acid digestibility than 21% forage NDF. Ruminal NDF digestibility was similar among dietary treatments. The increased milk production observed from feeding BMRCS in some studies may be explained by higher DM intake rather than increased total tract digestibility of the diets.     

Influence of corn variety and cutting height on nutritive value of silage fed to lactating dairy cows

Two corn varieties predicted to differ in digestibility were harvested at 2 cutting heights (10.2 or 30.5 cm) to determine effects on the nutrient content of the resulting silage, nutrient intake, nutrient digestibility, and production of lactating cows fed such corn silage originally harvested at two-thirds milk line. Acid detergent fiber (ADF) concentration was higher and in vitro true dry matter (DM) digestibility (IVTDMD) was lower for the variety predicted to have average digestibility. An interaction was observed between variety and cutting height because of decreased ADF and increased IVTDMD for the average digestibility variety cut at 30.5 vs. 10.2 cm; no differences were observed for the higher digestibility variety at each cutting height. When silages were fed to 32 Holstein cows in a 5-wk randomized design trial, DM intake, milk yield, and milk composition were similar. There was an interaction between variety and cutting height for DM intake and total tract apparent digestibility of DM, crude protein, and neutral detergent fiber because of lower intake and digestibility for the diets containing either the high cut, average quality variety or low cut, higher quality variety. These results suggest that increasing the cutting height to 30.5 cm does not improve silage quality or improve milk yield of cows. Although the 2 varieties selected for this trial were predicted to differ in digestibility, these differences were not great enough to influence milk yield or composition of lactating cows.     

Using brown midrib 6 dwarf forage sorghum silage and fall-grown oat silage in lactating dairy cow rations

Double cropping and increasing crop diversity could improve dairy farm economic and environmental sustainability. In this experiment, corn silage was partially replaced with 2 alternative forages, brown midrib-6 brachytic dwarf forage sorghum (Sorghum bicolor) or fall-grown oat (Avena sativa) silage, in the diet of lactating dairy cows. We investigated the effect on dry matter (DM) intake, milk yield (MY), milk components and fatty acid profile, apparent total-tract nutrient digestibility, N utilization, enteric methane emissions, and income over feed cost. We analyzed the in situ DM and neutral detergent fiber disappearance of the alternative forages versus corn silage and alfalfa haylage. Sorghum was grown in the summer and harvested in the milk stage. Oats were grown in the fall and harvested in the boot stage. Compared with corn silage, neutral detergent fiber and acid detergent fiber concentrations were higher in the alternative forages. Lignin content was highest for sorghum silage and similar for corn silage and oat silage. The alternative forages had less than 1% starch compared with the approximately 35% starch in the corn silage. Ruminal in situ DM effective degradability was similar, although statistically different, for corn silage and oat silage, but lower for sorghum silage. Diets with the alternative forages were fed in a replicated 3 × 3 Latin square design experiment with three 28-d periods and 12 Holstein cows. The control diet contained 44% (DM basis) corn silage. In the other 2 diets, sorghum or oat silages were included at 10% of dietary DM, replacing corn silage. Sorghum silage inclusion decreased DM intake, MY, and milk protein content but increased milk fat and maintained energy-corrected MY similar to the control. Oat silage had no effect on DM intake, MY, or milk components compared to the control. The oat silage diet increased apparent total-tract digestibility of dietary nutrients, except starch, whereas the sorghum diet slightly decreased DM, organic matter, crude protein, and starch digestibility. Cows consuming the oat silage diet had higher milk urea N and urinary urea N concentrations. Milk N efficiency was decreased by the sorghum diet. Diet did not affect enteric methane or carbon dioxide emissions. This study shows that oat silage can partially replace corn silage at 10% of the diet DM with no effect on MY. Brown midrib sorghum silage harvested at the milk stage with <1% starch may decrease DM intake and MY in dairy cows.     

Influence of fiber degradability of corn silage in diets with lower and higher fiber content on lactational performance,nutrient digestibility,and ruminal characteristics in lactating Holstein cows

The effect of neutral detergent fiber (NDF) degradability of corn silage in diets containing lower and higher NDF concentrations on lactational performance, nutrient digestibility, and ruminal characteristics in lactating Holstein cows was measured. Eight ruminally cannulated Holstein cows averaging 91 ± 4 (standard error) days in milk were used in a replicated 4 × 4 Latin square design with 21-d periods (7-d collection periods). Dietary treatments were formulated to contain either conventional (CON; 48.6% 24-h NDF degradability; NDFD) or brown midrib-3 (BM3; 61.1% 24-h NDFD) corn silage and either lower NDF (LNDF) or higher NDF (HNDF) concentration (32.0 and 35.8% of ration dry matter, DM) by adjusting the dietary forage content (52 and 67% forage, DM basis). The dietary treatments were (1) CON-LNDF, (2) CON-HNDF, (3) BM3-LNDF, and (4) BM3-HNDF. Data were analyzed as a factorial arrangement of diets within a replicated Latin square design with the MIXED procedure of SAS (SAS Institute Inc., Cary, NC) with fixed effects of NDFD, NDF, NDFD × NDF, period(square), and square. Cow within square was the random effect. Time and its interactions with NDFD and NDF were included in the model when appropriate. An interaction between NDFD and NDF content resulted in the HNDF diet decreasing dry matter intake (DMI) with CON corn silage but not with BM3 silage. Cows fed the BM3 corn silage had higher DMI than cows fed the CON corn silage, whereas cows fed the HNDF diet consumed less DM than cows fed the LNDF diet. Cows fed the BM3 diets had greater energy-corrected milk yield, higher milk true protein content, and lower milk urea nitrogen concentration than cows fed CON diets. Additionally, cows fed the BM3 diets had greater total-tract digestibility of organic matter and NDF than cows fed the CON diets. Compared with CON diets, the BMR diets accelerated ruminal NDF turnover. When incorporated into higher NDF diets, corn silage with greater in vitro 24-h NDFD and lower undegradable NDF at 240 h of in vitro fermentation (uNDF240) allowed for greater DMI intake than CON. In contrast, for lower NDF diets, NDFD of corn silage did not affect DMI, which suggests that a threshold level of inclusion of higher NDFD corn silage is necessary to observe enhanced lactational performance. Results suggest that there is a maximum gut fill of dietary uNDF240 and that higher NDFD corn silage can be fed at greater dietary concentrations.     

Performance of lactating dairy cows fed ryegrass silage and corn silage with ground corn, steam-flaked corn, or hominy feed

Forty-eight mid-lactation Holstein cows were used in a 6-wk completely randomized block design trial with a 4 × 3 factorial arrangement of treatments to determine the effects of feeding different proportions of corn silage and ryegrass silage with supplemental ground corn (GC), steam-flaked corn (SFC), and hominy feed (HF) on the performance of lactating dairy cows. Forage provided 49% of the dietary dry matter in the experimental diets, which were formulated to meet National Research Council requirements. Ryegrass silage provided 100, 75, 50, or 25% of the total forage dry matter, with corn silage supplying the remainder. There were no interactions between the proportion of forage provided by ryegrass silage and energy supplement. Dry matter intake and milk protein percentage decreased linearly with increasing proportions of ryegrass silage, but milk protein yield was similar among forage treatments. There were no differences among forage treatments in milk yield, milk fat percentage and yield, and energy-corrected milk yield. Dry matter intake was higher and there was a tendency for increased milk fat percentage for GC compared with SFC or HF. No other differences were observed in milk yield or composition among energy supplements. Plasma urea nitrogen and glucose concentrations were similar among treatments. Under the conditions of this trial, our results indicate that feeding a combination of corn silage and ryegrass silage is more desirable than feeding ryegrass silage alone, whereas supplementation with GC, SFC, or HF supports similar levels of milk production.     

Inclusion of brown midrib dwarf pearl millet silage in the diet of lactating dairy cows

Brown midrib brachytic dwarf pearl millet (Pennisetum glaucum) forage harvested at the flag leaf visible stage and subsequently ensiled was investigated as a partial replacement of corn silage in the diet of high-producing dairy cows. Seventeen lactating Holstein cows were fed 2 diets in a crossover design experiment with 2 periods of 28 d each. Both diets had forage:concentrate ratios of 60:40. The control diet (CSD) was based on corn silage and alfalfa haylage, and in the treatment diet, 20% of the corn silage dry matter (corresponding to 10% of the dietary dry matter) was replaced with pearl millet silage (PMD). The effects of partial substitution of corn silage with pearl millet silage on dry matter intake, milk yield, milk components, fatty acid profile, apparent total-tract digestibility of nutrients, N utilization, and enteric methane emissions were analyzed. The pearl millet silage was higher in crude protein and neutral detergent fiber and lower in lignin and starch than the corn silage. Diet did not affect dry matter intake or energy-corrected milk yield, which averaged 46.7 ± 1.92 kg/d. The PMD treatment tended to increase milk fat concentration, had no effect on milk fat yield, and increased milk urea N. Concentrations and yields of milk protein and lactose were not affected by diet. Apparent total-tract digestibility of dry matter decreased from 66.5% in CSD to 64.5% in PMD. Similarly, organic matter and crude protein digestibility was decreased by PMD, whereas neutral- and acid-detergent fiber digestibility was increased. Total milk trans fatty acid concentration was decreased by PMD, with a particular decrease in trans-10 18:1. Urinary urea and fecal N excretion increased with PMD compared with CSD. Milk N efficiency decreased with PMD. Carbon dioxide emission was not different between the diets, but PMD increased enteric methane emission from 396 to 454 g/d and increased methane yield and intensity. Substituting corn silage with brown midrib dwarf pearl millet silage at 10% of the diet dry matter supported high milk production in dairy cows. When planning on farm forage production strategies, brown midrib dwarf pearl millet should be considered as a viable fiber source.     

Comparison of a corn silage hybrid with high cell-wall content and digestibility with a hybrid of lower cell-wall content on performance of Holstein cows

We hypothesized that substituting a corn hybrid with high cell-wall content and high neutral detergent fiber (NDF) digestibility (HCW) for a hybrid with lower cell-wall content and lower NDF digestibility (LCW) would improve feed intake and milk production in lactating Holstein cows. There was a 3.6 percentage unit difference in NDF content and a 4.1 percentage unit difference in 30-h in vitro NDF digestion between the 2 corn hybrids. In trial 1, 40 cows (12 primiparous) ranging in milk production from 24.1 to 44.0 kg/d, following a 2-wk preliminary period, were used in a crossover design with 2-wk periods. Diets consisted of 45% corn silage (HCW or LCW), 10% alfalfa hay, and 45% concentrates. The DMI (25.4 vs. 24.2 kg/d) and 4% FCM yield (34.3 vs. 31.7 kg/d) were higher for cows fed the HCW diet compared with the LCW diet. When HCW was substituted for LCW on a DM basis, there was no relationship between pretrial milk yield (preliminary period) and subsequent response to HCW silage. In trial 2, 40 cows (8 primiparous) ranging in milk production from 20.6 to 49.0 kg/d, following a 2-wk preliminary period, were used in a crossover design with 2-wk periods. Diets consisted of the same LCW diet as trial 1 and a diet containing HCW at a concentration (40% of DM) that resulted in equal NDF content (30.8%) between the 2 diets (HCWN). The DMI (26.8 kg/d) was unaffected by diet, although there was a trend for greater DMI (% of BW) for cows fed the HCWN diet compared with LCW silage (4.24 vs. 4.12). Milk fat (3.91 vs. 3.79%) and 4% FCM yield (34.9 vs. 33.4 kg/d) were greater for cows fed HCWN vs. LCW diet. When HCW was substituted for LCW silage on an NDF basis, cows with greater milk production during the preliminary period had a greater milk response to HCW than lower-producing cows. Results of these trials supported our hypothesis that HCW corn silage results in greater DMI and milk yield than LCW silage, whether substitution occurs on a DM or NDF basis.     

Effect of applying bacterial inoculants containing different types of bacteria to corn silage on the performance of dairy cattle

This study examined the effect of applying different bacterial inoculants to corn silage at the time of ensiling on the performance of lactating dairy cows. Corn plants were harvested at 35% dry matter (DM), chopped, and ensiled in 2.4-m-wide bags after application of (1) no inoculant (CON); (2) Biotal Plus II (B2) containing Pediococcus pentosaceus and Propionibacteria freudenreichii; (3) Buchneri 40788 (BUC) containing Lactobacillus buchneri; or (4) Buchneri 500 (B500) containing Pediococcus pentosaceus and L. buchneri. All inoculants were supplied by Lallemand Animal Nutrition (Milwaukee, WI). Each of the 4 silages was included in separate total mixed rations consisting of 44% corn silage, 50% concentrate, and 6% alfalfa hay (DM basis). Fifty-two lactating Holstein cows were stratified according to milk production and parity and randomly assigned at 22 d in milk to the 4 dietary treatments. Cows were fed for ad libitum consumption and milked twice daily for 49 d. Dietary treatment did not affect intakes (kg/d) of DM (20.0), crude protein (CP; 3.7), neutral detergent fiber (NDF; 5.7), or acid detergent fiber (ADF; 3.6), or digestibility (%) of DM (73.9) or CP (72.4). However, NDF digestibility was lower in cows fed B2 compared with those fed other diets (45.3 vs. 53.0%). Consequently, cows fed B2 had lower digestible NDF intake (kg/d) than those fed other diets (2.5 vs. 3.0 kg/d). Dietary treatment did not affect milk yield (32.3 kg/d), efficiency of milk production (1.61), concentrations of milk fat (3.18%) and protein (2.79%), or yields of milk fat (1.03 kg/d) and protein (1.26 kg/d). Inoculant application to corn silage did not affect milk yield or feed intake of cows.     

Lactation response to soybean meal and rumen-protected methionine supplementation of corn silage-based diets

Corn silage, an important forage fed to dairy cows in the United States, is energy rich but protein poor. The objectives of this experiment were to investigate the effects on production of milk and milk components of feeding corn silage-based diets with 4 levels of dietary crude protein (CP) plus rumen-protected methionine (RPM). Thirty-six cows were blocked by days in milk into 9 squares and randomly assigned to 9 balanced 4 × 4 Latin squares with four 4-wk periods. All diets were formulated to contain, as a percent of dry matter (DM), 50% corn silage, 10% alfalfa silage, 4% soyhulls, 2.4% mineral-vitamin supplement, and 30% neutral detergent fiber. Supplemental RPM (Mepron, Evonik Corp., Kennesaw, GA) was added to all diets to maintain a Lys:Met ratio of 3.1 in digested AA. Ground high-moisture corn was reduced and soybean meal (SBM) plus RPM increased to give diets containing, on average, 11% CP (28% corn, 31% starch, 6% SBM, 4 g of RPM/d), 13% CP (23% corn, 29% starch, 10% SBM, 8 g of RPM/d), 15% CP (19% corn, 26% starch, 15% SBM, 10 g of RPM/d), and 17% CP (14% corn, 24% starch, 19% SBM, 12 g of RPM/d). Data from the last 14 d of each period were analyzed using the mixed procedures in SAS (SAS Institute Inc., Cary, NC). With the exception of milk fat and milk lactose content, we found no significant effects of diet on all production traits. We did note linear responses to dietary CP concentration for intake, production of milk and milk components, and MUN. Cows fed the 11% CP diet had reduced DM intake, lost weight, and yielded less milk and milk components. Mean separation indicated that only true protein yield was lower on 13% CP than on 17% dietary CP, but not different between 15 and 17% CP. This indicated no improvement in production of milk and milk components above 15% CP. Quadratic trends for yield of milk, energy-corrected milk, and true protein suggested that a dietary CP concentration greater than 15% may be necessary to maximize production or, alternately, that a plateau was reached and no further CP was required. Although diet influenced apparent digestibility of DM, organic matter, and neutral detergent fiber, digestibility did not increase linearly with dietary CP. However, we observed linear and quadratic effects of dietary CP on acid detergent fiber digestibility. As expected, we found a linear effect of dietary CP on apparent N digestibility and on fecal and urinary N excretion, but no effect of diet on estimated true N digestibility. Ruminal concentrations of ammonia, total AA, peptides, and branched-chain volatile fatty acids also increased linearly with dietary CP. Quadratic responses indicated that 14.0 to 14.8% CP was necessary to optimize digestion and energy utilization. Overall results indicated that, when RPM was added to increase Lys:Met to 3.1, 15% CP was adequate for lactating dairy cows fed corn silage diets supplemented with SBM and secreting about 40 kg of milk/d; N excretion was lower than at 17% CP but with no reduction in yield of milk and milk components.     

Effects of brown midrib 3 mutation in corn silage on dry matter intake and productivity of high yielding dairy cows

The effects of enhanced in vitro neutral detergent fiber (NDF) digestibility of corn silage on dry matter intake (DMI) and milk yield were evaluated using 32 Holstein cows in a crossover design with 28-d periods. At the beginning of the experiment, cows were 89 d in milk and yielded 45.6 kg/d of milk. Experimental diets contained either brown midrib (bm3) corn silage or isogenic normal corn silage (control) at 44.6% of DM. The NDF digestibility estimated by 30-h in vitro fermentation was higher for bm3 corn silage by 9.7 units. Contents of NDF and lignin were lower for bm3 corn silage by 1.8 and 0.8 units, respectively. Diets were formulated to contain 19% crude protein and 31% NDF and to have a forage to concentrate ratio of 56:44. Daily DMI, milk yield (3.5% fat-corrected milk), and solids-corrected milk were 2.1, 2.6, and 2.7 kg higher, respectively, for cows fed bm3 corn silage. The milk protein and lactose contents were greater for bm3 treatment, but milk fat content was not. Individual milk yield responses of the cows to bm3 treatment were positively related to pretrial milk yield, and DMI response tended to be positively related to pretrial milk yield. Enhanced in vitro NDF digestibility was associated with higher energy intake, which resulted in increased milk yield.     

Utilization of protein in red clover and alfalfa silages by lactating dairy cows and growing lambs

Feeding trials were conducted with lactating cows and growing lambs to quantify effects of replacing dietary alfalfa silage (AS) with red clover silage (RCS) on nutrient utilization. The lactation trial had a 2 × 4 arrangement of treatments: AS or RCS fed with no supplement, rumen-protected Met (RPM), rumen-protected Lys (RPL), or RPM plus RPL. Grass silage was fed at 13% of dry matter (DM) with AS to equalize dietary neutral detergent fiber (NDF) and crude protein contents. All diets contained (DM basis) 5% corn silage and 16% crude protein. Thirty-two multiparous (4 ruminally cannulated) plus 16 primiparous Holstein cows were blocked by parity and days in milk and fed diets as total mixed rations in an incomplete 8 × 8 Latin square trial with four 28-d periods. Production data (over the last 14 d of each period) and digestibility and excretion data (at the end of each period) were analyzed using the MIXED procedure of SAS (SAS Institute Inc., Cary, NC). Although DM intake was 1.2 kg/d greater on AS than RCS, milk yield and body weight gain were not different. However, yields of fat and energy-corrected milk as well as milk content of fat, true protein, and solids-not-fat were greater on AS. Relative to AS, feeding RCS increased milk and energy-corrected milk yield per unit of DM intake, milk lactose content, and apparent N efficiency and reduced milk urea. Relative to AS, apparent digestibility of DM, organic matter, NDF, and acid detergent fiber were greater on RCS, whereas apparent and estimated true N digestibility were lower. Urinary N excretion and ruminal concentrations of ammonia, total AA, and branched-chain volatile fatty acids were reduced on RCS, indicating reduced ruminal protein degradation. Supplementation of RPM increased intake, milk true protein, and solids-not-fat content and tended to increase milk fat content. There were no silage × RPM interactions, suggesting that RPM was equally limiting on both AS and RCS. Supplementation of RPL did not influence any production trait; however, a significant silage × RPL interaction was detected for intake: RPL reduced intake of AS diets but increased intake of RCS diets. Duplicated metabolism trials were conducted with lambs confined to metabolism crates and fed only silage. After adaptation, collections of silage refusals and excreta were made during ad libitum feeding followed by feeding DM restricted to 2% of body weight. Intake of DM was not different when silages were fed ad libitum. Apparent digestibility of DM, organic matter, NDF, and hemicellulose was greater in lambs fed RCS on both ad libitum and restricted intake; however, acid detergent fiber digestibility was only greater at restricted intake. Apparent and estimated true N digestibility was substantially lower, and N retention was reduced, on RCS. Results confirmed greater DM and fiber digestibility in ruminants and N efficiency in cows fed RCS. Specific loss of Lys bioavailability on RCS was not observed. Based on milk composition, Met was the first-limiting AA on both silages; however, Met was not limiting based on production and nutrient efficiency. Depressed true N digestibility suggested impaired intestinal digestibility of rumen-undegraded protein from RCS.     

Influence of corn silage hybrid type on lactation performance by Holstein dairy cows

The primary objective of this study was to determine lactation performance by dairy cows fed nutridense (ND), dual-purpose (DP), or brown midrib (BM) corn silage hybrids at the same concentration in the diets. A secondary objective was to determine lactation performance by dairy cows fed NutriDense corn silage at a higher concentration in the diet. One hundred twenty-eight Holstein and Holstein × Jersey cows (105 ± 38 d in milk) were stratified by breed and parity and randomly assigned to 16 pens of 8 cows each. Pens were then randomly assigned to 1 of 4 treatments. Three treatment total mixed rations (TMR; DP40, BM40, and ND40) contained 40% of dry matter (DM) from the respective corn silage hybrid and 20% of DM from alfalfa silage. The fourth treatment TMR had ND corn silage as the sole forage at 65% of DM (ND65). A 2-wk covariate adjustment period preceded the treatment period, with all pens receiving a TMR with equal proportions of DP40, BM40, and ND40. Following the covariate period, cows were fed their assigned treatment diets for 11 wk. nutridense corn silage had greater starch and lower neutral detergent fiber (NDF) content than DP or BM, resulting in ND40 having greater energy content (73.2% of total digestible nutrients, TDN) than DP40 or BM40 (71.9 and 71.4% TDN, respectively). Cows fed BM40 had greater milk yield than DP40, whereas ND40 tended to have greater milk yield and had greater protein and lactose yields compared with DP40. No differences in intake, component-corrected milk yields, or feed efficiency were detected between DP40, BM40, and ND40. Milk yield differences may be due to increased starch intake for ND40 and increased digestible NDF intake for BM40 compared with DP40. Intake and milk yield and composition were similar for ND40 compared with BM40, possibly due to counteracting effects of higher starch intake for ND40 and higher digestible NDF intake for BM40. Feeding ND65 reduced intake, and thus milk and component yields, compared with ND40 due to either increased ruminal starch digestibility or increased rumen fill for ND65. Nutridense corn silage was a viable alternative to both DP and BM at 40% of diet DM; however, lactation performance was reduced when nutridense corn silage was fed at 65% of DM.     

Effects of brown midrib corn silage on the energy balance of dairy cattle

Effects of genotype and level of intake on net energy for lactation values of corn silage were evaluated by indirect calorimetry in two experiments using lactating and dry, nonpregnant dairy cows. In experiment 1, six multiparous Holstein cows in early lactation were fed experimental diets containing either brown midrib (bm3) or isogenic normal corn silage. Dietary treatments were isogenic and bm3 diets fed ad libitum, and the bm3 diets restricted-fed. Dry matter (DM) intake was 2.4 kg/d greater for cows fed the bm3 diet ad libitum compared with cows fed the isogenic diet. Apparent digestibilities of DM, organic matter, neutral detergent fiber, and acid detergent fiber were greater for cows restricted-fed bm3 than the isogenic diet. In experiment 2, six dry, nonpregnant Holstein cows were fed maintenance diets containing either bm3 or isogenic corn silage. Apparent digestibilities of DM, organic matter, neutral detergent fiber, and acid detergent fiber were greater for cows fed bm3 compared with isogenic corn silage. Digestible energy and metabolizable energy were greater for maintenance diets containing bm3 compared with isogenic corn silage, respectively. These data indicate increased milk production seen in other studies is a result of increased DMI rather than an increase in energy efficiency. Increased organic matter digestibility of bm3 corn silage resulted in greater digestible energy and metabolizable energy values in cows fed at maintenance energy intake. However, calculated net energy for lactation values of bm3 and isogenic corn silages were similar at both productive and maintenance levels of feeding.     

Effects of varying dietary protein and energy levels on the production of lactating dairy cows

Forty-five multiparous and 18 primiparous Holstein cows were fed three levels of crude protein (CP), each at three levels of neutral detergent fiber (NDF), to identify optimal dietary CP and energy. Cows were blocked by parity and days in milk into seven groups of nine and randomly assigned to an incomplete 9 x 9 Latin square trial with four, 4-wk periods. Diets were formulated from alfalfa and corn silages, high-moisture corn, soybean meal, minerals, and vitamins. Forage was 60% alfalfa and 40% corn silage on all diets; NDF contents of 36, 32, and 28% were obtained by feeding 75, 63, and 50% forage, respectively. Dietary CP contents of 15.1, 16.7, and 18.4% were obtained by replacing high-moisture corn with soybean meal. Production data were from the last 2 wk of each period. Spot fecal and urine samples were collected from 36 cows to estimate N excretion using fecal indigestible acid detergent fiber (ADF) and urinary creatinine as markers. There were no interactions (P > or = 0.08) between dietary CP and NDF for any trait; thus, effects of CP were not confounded by NDF or vice versa. Intake of DM and fat yield were lower on 15.1% CP than at higher CP. There were linear increases in milk urea and urinary N excretion and linear decreases in N efficiency with increasing CP. Increasing CP from 15.1 to 18.4% reduced milk N from 31 to 25% of dietary N, increased urinary N from 23 to 35% of dietary N, and reduced fecal N from 45 to 41% of dietary N. Decreasing NDF gave linear increases in BW gain, yield of milk, protein, true protein, lactose, and SNF, and milk/DM intake and milk N/N intake, and linear decreases in milk urea. However, fat yield was lower on 28% than 32% NDF. Reducing NDF from 36 to 28% increased purine derivative excretion by 19%, suggesting increased microbial protein. Increasing CP by adding soybean meal to diets fed cows averaging 34 kg/d of milk increased intake and fat yield but depressed N efficiency. Increasing dietary energy by reducing forage improved milk yield and efficiency and decreased excretion of environmentally labile urinary N.     

Partial replacement of corn silage with whole-plant soybean and black oat silages for dairy cows

This study aimed to evaluate the effects of partially replacing corn silage (CS) with whole-plant soybean silage (SS) or black oat silage (OS) on nutrient intake and digestibility, in vitro neutral detergent fiber degradability of silages, feeding behavior, rumen fermentation, and performance of dairy cows. Twenty-four lactating Holstein cows (6 of which were rumen-cannulated) with 32.5 ± 4.92 kg/d milk yield, 150 ± 84.8 days in milk, and 644 ± 79.0 kg of body weight were used in a 3 × 3 Latin square design to evaluate the following treatments: (1) corn silage diet (CSD): using corn silage as the only forage source in the diet [48% dietary dry matter (DM)]; (2) whole-plant soybean silage diet (SSD): SS replacing 16% of corn silage from CSD; and (3) black oat silage diet (OSD): OS replacing 16% of corn silage from CSD. The inclusion of OS and SS decreased intakes of DM, organic matter, and crude protein. Corn silage had the greatest in vivo effective degradability of DM, and SS had the least effective degradability of neutral detergent fiber. The OSD treatment decreased milk and protein yields, whereas SSD increased rumen ammonia nitrogen concentration compared with the other diets. Cows fed OSD exhibited a greater preference for feed with small particles (<4 mm) compared with those fed SSD. Cows fed treatments containing either SS or OS at the expense of CS had increased rumination and chewing activities. Although replacing CS with OS and SS reduced feed intake, SS had no effect on productive performance of dairy cows.     

Effects of feeding diets based on silage from corn hybrids that differed in concentration and in vitro digestibility of neutral detergent fiber to dairy cows

A dual-purpose hybrid and a hybrid selected for high neutral detergent fiber (NDF) concentration were harvested as corn silage. The dual-purpose silage (DPCS) had 42% NDF and 35.4% in vitro (30 h) NDF digestibility and the high fiber silage (HFCS) had 49% NDF and 40.1% in vitro NDF digestibility. Two diets (dry matter basis) had 45% DPCS or HFCS and 46% corn grain-based concentrate (dietary NDF was 29 and 32%, respectively), a third diet had 33% HFCS and 58% corn-based concentrate (27% dietary NDF), and a fourth diet had 33% DPCS and 58% concentrate that contained soybean hulls (32% dietary NDF). All diets contained 9% alfalfa silage. Diets were fed to eight midlactation Holstein cows in a 4 x 4 Latin square with 28 d periods. No differences among treatments were observed for milk yield (34.1 kg/d), dry matter intake (23.7 kg/d), and yield and concentration of milk protein. Cows fed the diet with 33% HFCS tended to have lower milk fat percentage than cows fed the 45% DPCS diet. Total digestible nutrients (measured using total collection) tended to be lower for the 33% DPCS diet than for the 45% DPCS diet. In vivo digestibility of NDF tended to be lower for the 33% HFCS diet than the 45% DPCS diet, but digestibility of starch in the two diets with HFCS was higher than the 45% DPCS diet. The lack of any substantial differences in responses suggest that the HFCS was equal to the DPCS when fed at 45% of the diet dry matter (53.5% total forage). When HFCS replaced DPCS so that NDF was similar between diets, milk fat percentage was reduced and ruminal propionate was increased. Increasing dietary NDF by adding soybean hulls to a diet based on DPCS reduced digestibility of dry matter, organic matter, and protein, and resulted in lower energy balance than the 45% DPCS diet.