基于幽门螺旋杆菌检测的自动封闭袋设计

介绍了幽门螺旋杆菌检测自动封闭袋的设计。首先,介绍幽门螺旋杆菌与多种疾病的密切相关性及其检测方法,指出常用的呼气检测法存在的问题。提出幽门螺旋杆菌检测自动封闭袋的设计的的作用和优点,接着对幽门螺旋杆菌检测自动封闭袋结构组成中的吹气管、橡胶密封环、通气杆、袋体、粘接环等5部分的结构进行详细的介绍。从而明晰自动封闭袋的设计原理,即由自动封闭袋的袋体在吹入气体压力的作用下拉动通气杆,使得通气杆前端部连接的橡胶密封环在拉力的作用下堵住通气杆进气口,实现袋体的自动封闭。最后,对自动封闭袋进行测试验证,验证结果表明应用自动封闭袋进行检测的准确性为100%。最后,介绍幽门螺旋杆菌检测自动封闭袋的应用,以及未来发展前景。     

根除幽门螺旋杆菌与不同亚型老年功能性消化不良疗效的关系

目的:分析根除幽门螺旋杆菌(Helicobacter pylori,HP)与不同亚型老年功能性消化不良(Functional dyspepsia,FD)疗效的关系。方法 :2013年6月—2015年6月收治184例HP阳性老年FD患者,按照随机数字表法分为抗HP组、常规组各92例。均予FD常规治疗,抗HP组加用HP根除治疗,观察抗HP组HP根除成功情况,比较不同亚型患者治疗1个月后、治疗1年后疗效及复发情况。结果 :抗HP组治疗1个月后、治疗1年后FD治疗总有效率分别为78.26%、72.83%,均高于常规组的59.78%、57.61%;HP根除成功组治疗1个月后、治疗1年后总有效率分别为95.24%、98.21%,均高于HP根除失败组的68.42%、46.15%;抗HP组EPS亚型患者治疗1个月后、治疗1年后总有效率分别为86.00%、80.00%,均高于PDS亚型的69.05%、64.29%,差异有统计学意义(P<0.05)。抗HP组、常规组患者不良反应发生率分别为5.43%、6.52%,组间比较差异无统计学意义(P>0.05)。结论 :根除HP可提高EPS型老年PD患者临床疗效。     

13C同位素示踪ITER碳壁腐蚀的方法综述

碳原子序列较低,可耐高温,具有高导热性和优良的等离子体相容性,十分适合作为托卡马克(ITER)中的面向等离子体材料(Plasma Facing Materials,PFMs).但碳壁在面临聚变装置内部复杂的使用环境时,会遭受等离子体粒子(尤其是氢同位素D、T粒子)的轰炸、瞬态和稳态高热负荷和电磁效应等伤害,形成的碳共沉...     

同位素示踪法测定~(125)I-NGF在小鼠体内的血药浓度变化

本文涉及应用Idogn法对神经生长因子(NGF)进行125I标记并利用同位素示踪与电泳分离相结合的方法测定125I-NGF在小鼠体内的血药浓度与时间变化关系。结果显示,静脉注射125I-NGF在小鼠体内的代谢规律符合二房室开放模型。分布相半衰期为0.13h,消除相半衰期为3.68h,125I-NGF在小鼠体内分布和消除均较快,清除率为0.125L/h/Kg,表观分布容积为0.697L/kg,曲线所围面积为16.01μg.h/L。     

同位素示踪法研究~(125)Ⅰ-NGF在小鼠体内的吸收、分布及排泄

本文应用放射性同位素示踪法与电泳分离法相结合的方法研究125I-NGF在小鼠体内的吸收、分布和排泄。结果显示:静脉注射和肌肉注射125I-NGF在小鼠体内的代谢规律均符合二房室开放模型。肌肉注射10μg/kg,20μg/kg,40μg/kg 3个剂量达峰时间分别为0.71h,1.66h,3.78h;峰浓度分别为1.74ng/mL,2.82ng/mL,4.23ng/mL;曲线所围面积分别为8.44μg/h/L,21.6μg/h/L,43.12μg/h/L,消除相半衰期分别为4.83h,4.49h,4.43h。肌肉注射10μg/kg125I-NGF生物利用度为52.7%。静脉注射125I-NGF 10μg/kg,分布相半衰期为0.13h,消除相半衰期为3.68h,表观分布容积为0.697L/kg,曲线所围面积为16.01μg/h/L。125I-NGF在所测的14种组织中均有分布,其中肾、胃、肠中放射性浓度最高,脑和脊髓最少。肌肉注射125I-NGF主要随尿液排出体外。     

汽车罩光漆的同位素比值分析及保存条件影响研究

本文通过检验汽车油漆最外层罩光漆的同位素实现不同样品的区分,并考察保存条件对样品同位素的影响。实验共收集了10种汽车罩光漆样品,分别在室温避光、60℃高温、-20℃低温和强紫外线条件下保存6个月,然后用元素分析仪-同位素比值质谱(EA-IRMS)检测样品的碳、氢、氧同位素比值。结果表明：油漆样品在不同保存条件下发生碳、氢、氧同位素分馏,样品的δ¹³C值在高温和低温保存后向负值偏移,强紫外线照射的影响微弱;δ²H值在3种条件下均向正值偏移,受紫外线照射影响最大;δ¹⁸O值在3种条件下均向负值偏移,但不同种类树脂样品之间δ¹⁸O值的偏移幅度不同,在3类样品中,聚甲基丙烯酸甲脂类树脂样品分子中由于氧含量最少其偏移值最小。稳定同位素分析方法可为油漆样品分析提供高区分度的三维指纹,在油漆识别与区分方面具有较大的潜力,可用于油漆物证的溯源分析。     

ZTQ-201型~(13)C检测仪的研制

研制成功ZTQ 2 0 1型13C检测仪 ,该仪器采用色谱分离和质谱测量原理 ,测定人体呼出气中CO2 的13C/ 12 C比值 ,测量精度 (δ)优于 0 5‰。本文介绍仪器的工作原理、组成和性能。     

示踪粒子在离心泵内流场跟随性的影响因素分析

以修正后适用于低雷诺数下粒子在离心泵叶轮内运动方程为基础,给出离心泵内流场粒子成像测速(Particle imagevelocimetry,PIV)测试中示踪粒子跟随性的数学表述。据此对离心泵内流场中示踪粒子的运动进行数值计算,讨论粒子跟随性对粒子物理特性和离心泵内流场性质的依赖关系。并在现有几种模型基础上对比讨论各项力对粒子跟随性的贡献,指出各模型的优缺点。计算结果表明,密度接近流体的密度的中性悬浮粒子跟随性较好;对于密度较大的粒子只有粒子直径足够小才能跟随到一定范围内湍流角频率的流动;粒子径向跟随性比周向复杂得多;在离心泵叶轮内低速漩涡区粒子跟随性较差,尤其是在径向方向上;离心泵叶轮旋转引起的离心力和科氏力以及流道弯曲产生的离心力对粒子跟随性影响很大。通过了解和掌握这些因素的影响,对于离心泵内流场PIV测试中采用合适的示踪粒子和控制粒子跟随性误差具有重要的理论指导意义。     

负热电离飞行时间质谱仪及其用于硼酸中硼同位素比值的测定

在自行研制的热电离飞行时间质谱仪(TI-TOF-MS)基础上,通过对仪器的重新设计和电源的改造,研制了负离子热电离飞行时间质谱仪(NTI-TOF-MS)。文中介绍NTI-TOF-MS仪器的结构及性能特点,通过对仪器性能进行测试,获得了仪器的分辨率,仪器在m/z为219amu处的分辨率为1369。文中应用NTI-TOF-MS法测量了核纯级硼酸样品中硼的同位素比值,测量的结果为¹⁰B/¹¹B=0.2403±0.0007。     

气相色谱-热转换-同位素比值质谱法测定单体氢同位素稳定性的影响因素分析

采用气相色谱-热转换-同位素比值质谱法（GC-TC-IRMS）测定有机单体化合物氢同位素。实验中每测5个样品,需要测定1个实验室工作标样（正构烷烃C₂₁、C₂₅、C₂₇、C₂₉、C₃₁及C₃₃）。通过逾4个月跟踪分析近1100个样品发现,采用GC-TC-IRMS法测定单体氢同位素时,从使用一个新的裂解管开始,实验室工作标样中不同碳数正构烷烃达到长期稳定状态所需的时间有所差异,C₂₁正构烷烃达到稳定状态最快。实验表明,严格控制仪器测试条件,选用优质材料裂解管,样品气相色谱图干净、目标化合物峰分离良好,是保证GC-TC-IRMS测定有机单体氢同位素结果准确、稳定的重要条件。     

催化裂解制乙烯液相产物及其原料GC/MS分析

采用气相色谱-质谱联用技术对催化裂解制低碳烯烃过程中的裂解原料及液相产物进行定性定量分析,比较原料与液相产物在化学组成及相对含量上的差异;采用两种不同工艺裂解正十二烷,对其液相产物进行GC/MS分析,分析结果表明催化裂解过程比热裂解过程更有利于正十二烷烃的裂解,而热裂解过程比催化裂解过程更易发生α断裂。分析数据为评价催化剂性能,研究裂解机理,改进工艺条件提供参考。     

An improved model for the frequency estimate of mass-loaded plates by a combined use of equivalent center mass and stiffness factors

In this paper, an improved model is developed to determine the frequency of vibrating plates carrying multiple masses at various positions. By virtue of an equivalent-center method, the frequencies of the plates carrying multiple masses can be predicted by using merely the data obtained earlier for the same plate with each corresponding mass component. Further accounting the change of the strain energy into the model and by introducing respective stiffness ratios, one can obtain a quick and better estimation of the loaded plates with various boundary conditions. The proposed model is validated through experimentation of a rectangular clamped plate carrying concentrated masses. Analytical and experimental results for the plate system are compared and discussed. It is found that the change in the strain energy should be incorporated in the model, especially for cases of large masses placed on a thin plate, in order to predict well the natural frequency of the amalgamated system from those of the component systems. The excellent performance of the proposed model is due to the fact that the effects of both the different masses and their locations on the frequency have been accounted for, implicitly, in the respective equivalent center mass and stiffness factors. Although the model considered is rather simple, the proposed methodology can be extended to plates with other geometry and configurations.     

Detection of sulfur in fixed and embedded rat peritoneal mast cell granules by X-ray energy dispersive spectroscopy

Purified rat peritoneal mast cells contained 3.3 × 10^?5 gm SO₄ and 2.2 × 10^?8 gm Ca/10⁶ cells. The molar ratio of S/Ca in the whole cell was 600:1. Frozen thin sections of unfixed mast cells contained only sulfur (S) in the granules when examined by X-ray energy dispersive spectroscopy (EDS). Mast cells fixed in 3% glutaraldehyde and 1.5% formaldehyde in 75% ethanol (Et/Ald) or in mixed buffered aldehydes and embedded in Epon 812 or the low viscosity resin diepoxyoctane (DEO) contained S in all granules and Ca in some of the granules measured. Neither element was found in the nucleus, cytoplasm, or resin. Isolated, Et/Ald fixed and embedded granules also contained S. The presence of Ca in the granules was artifactual in that the Ca was absorbed from water in the trough of the diamond knife and/or from the filter paper used to blot the sections dry. This phenomenon was investigated further. Sections of Et/Ald fixed and embedded mast cells were incubated with 5 × 10^?6 to 10^?2 M CaCl₂. Ca was detectable in 100% of the granules incubated at concentrations ≥ 10^?4 M and reached a constant S/Ca ratio of 2.0 at concentrations ≥ 10^?3 M. Ca was not detectable in the nucleus, cytoplasm, or resin at 10^?2 M. A plot of S versus Ca counts from the granules of cells incubated with 10^?2 M CaCl₂ was linear with a slope of 2.0 and a correlation coefficient of 0.88. Et/Ald fixed cells incubated with distilled H₂O had fewer granules containing Ca, 10%, than unincubated cells, 77%. Further, H₂O removed all Ca from Et/Ald fixed cells embedded in DEO. These studies show that S, which is present as SO₄ on the proteoglycan heparin, is readily detectable by X-ray EDS in fixed and embedded cells. An artifact of the technique is that weak anionic sites, which are most probably carboxyl groups on the proteoglycan, can bind the divalent cation Ca and cause spurious localization.