玉米赤霉烯酮时间分辨荧光试纸条的制备及特性研究

研究制备一种基于时间分辨荧光免疫层析法的玉米赤霉烯酮(ZEN)快速定量检测试纸条.采用铕纳米微球作为荧光探针,标记抗ZEN的单克隆抗体,应用竞争抑制原理建立免疫层析定量方法并对其进行方法学考核.结果表明:ZEN荧光试纸条的灵敏度为0.1 ng/mL,线性范围为0.1～5.0 ng/mL,玉米、小麦阴性样品的加标回收率在...     

量子点微球免疫层析试纸条检测鸡蛋中恩诺沙星的性能评价

目的:现场快速筛查鸡蛋中恩诺沙星。方法:按《食品安全国家标准食品中41种兽药最大残留限量》要求，依据国家市场监督管理总局2023年1月发布的《关于规范食品快速检测使用的意见》,制备3个浓度水平的恩诺沙星加标样本。50份空白样本和50份标准限量要求0.5倍浓度水平(5μg/kg)的加标样本用以评价快检方法的假阳性率；50份标准限量要求1倍浓度水平(10μg/kg)的加标样本用以评价快检方法的假阴性率。50份实际鸡蛋样本用量子点微球免疫层析试纸条与HPLC-MS/MS方法分别进行检测。结果:量子点微球免疫层析试纸条可以满足国家限量要求，假阴性率为0%,假阳性率为0%。实际样本检测时，量子点微球免疫层析试纸条与HPLC-MS/MS方法的结果符合率高。结论:借助于便携式读取仪，量子点微球免疫层析法可获得数字化的准确结果，适合鸡蛋中风险因子的大批量样本现场快速筛查。     

玉米赤霉烯酮时间分辨荧光试纸条的制备及特性研究（网络首发、推荐阅读）

研究制备一种基于时间分辨荧光免疫层析法的玉米赤霉烯酮（ZEN）快速定量检测试纸条。采用铕纳米微球作为荧光探针,标记抗ZEN的单克隆抗体,应用竞争抑制原理建立免疫层析定量方法并对其进行方法学考核。结果表明：ZEN荧光试纸条的灵敏度为0.1 ng/mL,线性范围为0.1~5.0 ng/mL,玉米、小麦阴性样品的加标回收率在93.45%~112.20%之间,对于同一个样品5次重复测定的变异系数小于15%。ZEN快速定量检测试纸条具有灵敏度高、反应时间短、准确定量、稳定性好等技术特点,适用于谷物及制品中玉米赤霉烯酮的快速定量检测。     

量子点荧光微球免疫法定量检测小麦中黄曲霉毒素B1

摘 要: 目的 建立量子点荧光微球免疫法快速检测小麦中黄曲霉毒素B1的方法。方法 采用量子点荧光微球作为荧光标记物,与黄曲霉毒素B1的单克隆抗体偶联,构建量子点荧光微球探针。优化缓冲液pH、抗体最小标记量、荧光探针用量和包被抗原浓度等实验条件,建立检测卡上T线和C线信号峰值面积的比值与样本中黄曲霉毒素B1浓度的关系,构建定量标准曲线。针对小麦样品,将该检测方法与时间分辨荧光定量检测方法进行比较。结果 本研究建立的荧光定量免疫层析检测方法最佳反应条件为：pH 7.5磷酸钠缓冲液,抗体标记量为20 μg,荧光探针用量为4.0 μL,抗原质量浓度使用0.40 mg/mL。小麦中黄曲霉毒素B1的定量检测线性范围为0.05 μg/kg-25 μg/kg,其线性拟合方程为Y= -0.6058X + 12.523（r2=0.9994）,检出限为0.02 μg/kg,定量限为0.05 μg/kg。加标回收率在91.50%~115.00%之间,变异系数在1.88%~4.35%之间。结论 本研究建立的荧光定量免疫层析方法快速、准确、稳定性好、可靠性高,适用于小麦中黄曲霉毒素B1的现场快速检测。     

量子点标记免疫亲和凝胶柱快速检测动物组织中恩诺沙星

目的:建立一种检测动物组织中残留的兽药恩诺沙星(ENR)的量子点标记免疫亲和凝胶柱快速检测新方法。方法:将恩诺沙星特异性抗体偶联到溴化氢活化的琼脂糖凝胶上,制备抗体胶,将抗体胶装入标准的1mL SPE固相萃取柱制成恩诺沙星免疫亲和凝胶检测柱。采用混合酸酐法制备恩诺沙星包被抗原(ENR-OVA),采用活化酯法将ENR-OVA偶联到水溶性量子点(QDs)上制备荧光信号探针QDs-OVA-ENR。将待测样品和荧光信号探针QDs-OVA-ENR分别加入检测柱中,基于抗原抗体的竞争结合反应,样品中的恩诺沙星和荧光信号探针QDs-OVA-ENR竞争结合检测柱中抗体。通过目测检测柱体的荧光强弱,定性半定量检测恩诺沙星的含量。结果:该量子点标记恩诺沙星免疫亲和凝胶检测柱的检测限(LOD)为2μg/L,对食源性动物组织样品的检测限为20μg/kg。检测过程不超过5 min。结论:该方法操作简便,灵敏度高,检测时间短,结果易于判断,可满足食源性动物组织中恩诺沙星残留的现场快速检测要求。     

时间分辨荧光免疫层析快速检测牛奶中地塞米松

目的 建立基于时间分辨荧光微球对牛奶中的地塞米松残留进行快速检测的方法,并对该方法性能进行评估。方法 使用地塞米松抗体标记时间分辨荧光微球,分别采用湿法和干法制备检测试纸条,比较两种方法的灵敏度,同时确定微球的最佳抗体标记量,并对试纸条的重复性、检测灵敏度进行评估。结果 以20μg抗体/mg微球的标记量,湿法制备的试纸条检测灵敏度更佳。该方法重复性检测中变异系数(coefficientof variation, CV)为8.2%,地塞米松溶液的检出限为0.07 ng/mL,检测牛奶中地塞米松的半抑制浓度(half maximal inhibitory concentration, IC50)为0.24 ng/mL。结论 该方法具有测试简单、灵敏度高等特点,制备的试纸条可满足牛奶中地塞米松残留的检测要求。     

聚集诱导发光微球免疫层析试纸条定量检测水产品中孔雀石绿

目的 建立一种用于定量检测水产品中孔雀石绿的高灵敏免疫层析检测新方法。方法 以聚集诱导发光荧光微球为标记探针,制备荧光免疫层析试纸条,通过T/C比值法构建定量检测孔雀石绿的定量标准曲线,并实现水产品中孔雀石绿的高灵敏、定量检测。结果 本方法定量检测水产品中孔雀石绿具有较高的检测灵敏度,其50%抑制率达到0.17μg/kg,最低检出限为0.05μg/kg。样本加标0.25、0.50、1.00μg/kg的平均回收率介于116.01%～122.76%,变异系数介于8.66%～11.71%(n=10),表明所建立的荧光免疫层析方法定量检测水产品中孔雀石绿具有较好的准确性、精密度。通过检测30份孔雀石绿阴性以及12份阳性的水产样本,结果表明所建立的荧光免疫层析方法无假阳性,且检测孔雀石绿含量与液相色谱-串联质谱法具有良好的一致性(线性相关系数为0.9695)。结论 本研究以聚集诱导发光荧光微球为新型标记探针,建立了一种高灵敏检测水产品中孔雀石绿的定量方法,为水产品中孔雀石绿的筛查检测提供了技术支撑。     

呕吐毒素时间分辨荧光免疫层析法的建立与评价

构建并评价了呕吐毒素(Deoxynivalenol,DON)时间分辨荧光免疫层析法(TRFIA)。以Eu3+螯合物的纳米微球为荧光探针标记抗DON单抗,采用竞争抑制建立免疫层析定量方法,优化了微球与抗体标记量,检测的环境温度、反应时间、加样体积及缓冲体系;研究了方法的灵敏度、精密度及准确度。结果表明,每100 μL荧光微球结合纯单抗50 μg,最佳划膜浓度为1.0 mg/mL,最佳测定条件为室温(25±2)℃,10 min,加样体积100 μL,PBS (0.01 mol/L,pH7.2)的缓冲体系,方法的灵敏度为0.25 ng/mL,线性范围为0.5~25.0 ng/mL,玉米、小麦阴性样本(加标浓度200、500、1000、2000 μg/kg)平均加标回收率为91.4%~109.3%,6种典型样本经TRFIA与免疫亲和净化-高效液相色谱法(IAC-HPLC)同时测定DON含量,相关系数r达0.9754。TRFIA具有灵敏度高、速度快、定量准等技术特点,适用于谷物及制品中DON的快速定量。     

一种新型定量检测恩诺沙星量子点免疫层析方法的建立

恩诺沙星作为一种应用较为广泛的抗生素,因在实际抽检中出阳率较高引起了广泛的重视。本研究建立了一种可以快速定量检测恩诺沙星,采用量子点进行标记和示踪的免疫层析方法。通过对新型的纳米荧光材料量子点结合抗原抗体等活性材料的技术研究、样本前处理试剂的研发、浓度的筛选优化等技术实现量子点材料与食品基质的配适,建立定量检测恩诺沙星免疫层析的平台。通过对试剂盒整体的相关优化,本检测方法线性范围为2~1000 ng/mL,最低检测限为1.80 ng/mL,精密度可控在10%以内,热加速稳定性为37 ℃可稳定放置10 d,性能优越。与传统液相色谱方法比较,简便快捷,整体检测时间可控在20 min内,检测结果相关性较好,相关系数为0.98。本研究弥补国内对量子点与免疫层析技术相结合定量检测食品类抗生素残留方面研究的空缺,可实现大批量现场原材料的快速筛查,推动食品快检技术的发展。     

呋喃西林代谢物单克隆抗体及荧光免疫层析试纸条的制备

为建立一种基于荧光免疫层析技术的呋喃西林代谢物的快速检测方法,采用活性酯法合成呋喃西林代谢物（氨基脲盐酸盐）（semicarbazide hydrochloride,SEM）人工抗原,并获得SEM的单克隆抗体,通过将量子点微球（quantumdot submicrobeads,QBs）与SEM偶联,得到QBs探针,制备荧光免疫层析试纸条。结果表明：SEM免疫荧光层析试纸条检测鱼肉组织的检测限为0.247 μg/L,不同添加质量浓度的回收率均在70%～120%之间,批内、批间变异系数均在15%以下,符合我国动物源食品中兽药残留检测方法相关指导文件的规定,且在2～8 ℃条件下可以保存6 个月以上,稳定性良好。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Chlorohydrins of bisphenol A diglycidyl ether (BADGE) and of bisphenol F diglycidyl ether (BFDGE) in canned foods and ready-to-drink coffees from the Japanese market

BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GCMS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl and BFDGE.HCl.H₂O at a level of 1.5mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.     

European priorities for research to support legislation in the area of food contact materials and articles

A strong science base is required to underpin the planning and decision-making process involved in determining future European community legislation on materials and articles in contact with food. Significant progress has been made in the past 5 years in European funded work in this area, with many developments contributing to a much better understanding of the migration process, and better and simpler approaches to food control. In this paper this progress is reviewed against previously identified work-areas (identified in 1994) and conclusions are reached about future requirements for R&D to support legislation on food contact materials and articles over the next 5 or so years.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the