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1.
KHESLLER PATRÍCIA OLAZIA JULIANA SOUZA DA SILVA GUILHERME SIRIMARCO DE SOUZA TONELLI SÔNIA NAIR BÁO 《Biocell》2022,46(1):275-283
In this study, the sperm ultrastructure of three species of Idarnes genus was investigated using light and transmission electron microscopy. Spermatozoon morphology of the three species was similar to that of most Chalcidoidea, with helicoidally twisted nucleus and flagellum. The head region consists of an acrosome and a nucleus; the nucleus-flagellum transition region characterized by the presence of mitochondrial derivatives and the centriolar adjunct; a flagellum region, which includes the axoneme with microtubular arrangement 9 + 9 + 2 and two mitochondrial derivatives. However, the sperm of these three species exhibit features that discriminate one species from each other: (1) only one species, Idarnes sp. 2 (carme group) exhibited an extracellular sheath surrounding the anterior portion of the nucleus, which extends to the anterior region of the flagellum, but it did not present filaments; (2) the acrosome in the three species was quite different, Idarnes sp. 1 and Idarnes sp. 2 (carme group) has two compartments (acrosomal and subacrosomal vesicles) while Idarnes sp. 3 (flavicollis group) has a third compartment (perforatorium); (3) the centriolar adjunct elongated and its location among the mitochondrial derivatives is similar for the three species analyzed; (4) mitochondrial derivatives differ between the species, with triangular (Idarnes sp. 1 and sp. 3) and elongated or flat shaped (Idarnes sp. 2) appearance. These data shows that sperm structure may differ within the same genus and confirms the potential of these cells in phylogenetic and taxonomic analyses in the Chalcidoidea superfamily, as well as in Hymenoptera in general. 相似文献
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3.
LUIS A. CARO NATALIA SANTECCHIA PABLO A. MARINANGELI NÉSTOR R. CURVETTO LUIS F. HERNÁNDEZ 《Biocell》2003,27(3):311-318
The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned medium, but with 0.15 mg.L-1 of BAP. After 2 months, microshoots with 2-3 leaves were selected and cultured in BTMm-agar, supplemented with 5 mg.L-1 IBA or in liquid BTMm on perlite and, in the presence or absence of A. rhizogenes (Ar) and in combination with 3 mg.L-1 IBA. Rooting in P. chilensis reached 100.0% when Ar infection was produced in the presence of IBA, increasing both, the number and dry weight of roots. In N. alpina, 90.0% of rooting efficiency was obtained when Ar infection was produced in liquid culture and in the absence of auxin. 相似文献
4.
Kadsura coccinea (Lem.) is a woody wine plant with a peculiar fruit enriched in important health-promoting
compounds. The non-editable part of the fruit, i.e., the seed and peel, represents more than 60% of the fruit and is
considered a biowaste. This significantly restricts the development of the K. coccinea fruit industry. Clarifying the
metabolic components of the different fruit parts can help to improve the utilization rate and valorization of
K. coccinea. Herein, we evaluated K. coccinea fruit peel, pulp, and seed using widely-targeted metabolomics and
quantified a set of 736 bioactive compounds from 11 major metabolite classes. The most prominent metabolite classes
included lipids, amino acids, flavonoids, and lignans. Furthermore, our results emphasized a significant accumulation
of flavonoids in pulp tissues, while alkaloids and lignans were abundant in peel and seed tissues, respectively. A total
of 183 metabolites were differentially accumulated among the three tissues. Procyanidin C2, rutinoside,
2-hydroxyoleanolic acid, 5-hydroxymethyluracil, nootkatol, isoquercitrin, isohyperoside, quercetin-7-O-glucoside,
hyperin, and rutin showed elevated accumulation in the peel. In the seed, kadsuralignan G, kadcoccilactone A,
kadsuralignan H, lysoPE 20:5, iso-schisandrin ethyl alcohol, and kadangustin were significantly enriched. Our results
highlight the diverse metabolome composition of K. coccinea fruit parts, which can be further exploited for its
valorization in various industries. 相似文献
5.
GRACIELI DALLA NORA TAMARA PASTORI HAYWOOD DAIL LAUGHINGHOUSE IV THAIS SCOTTI DO CANTO-DOROW SOLANGE BOSIO TEDESCO 《Biocell》2010,34(3):95-102
Mikania glomerata is a plant used in Brazilian traditional medicine, known as ‘guaco’. It possesses anti-inflammatory properties and the aqueous extracts of its leaves are indicated for the treatment of diseases of the respiratory tract. This study aimed at evaluating the antiproliferative and genotoxic effect of Mikania glomerata leaf infusions on the cell cycle of onion. The material used was collected in the native environment from Rio Grande do Sul State, Brazil. Aqueous extracts through infusions were prepared in two concentrations: 4g/L (usual concentration) and 16g/L (4x more concentrated) of each of the populations. Two groups of four onion bulbs for each plant population were used plus a control group. The rootlets were fixed in ethanol-acetic acid (3:1), conserved in ethanol 70% and slides were prepared using the squashing technique colored with orcein 2%. The cells were observed and analyzed during cell cycle. Per group of bulbs, 2000 cells were analyzed, and the mean values of the cell number of each of the phases of the cell cycle were calculated, determining the mitotic index (MI). Statistic analyses of the data were carried out by the x2 (p= 0.05) test. We conclude that M. glomerata presents both antiproliferative and genotoxic activity. 相似文献
6.
WAN ZHANG GUIYAN YANG YUNLIN ZHAO ZHENGGANG XU HUANG HUIMIN JIAKANG ZHOU 《Biocell》2022,46(3):803-819
Broussonetia kazinoki × Broussonetia papyrifera (ZJGS) is a hybrid species in Moraceae family, which has a very complicated hybrid origin. The excellent characteristics of fast growth, strong soil and water conservation ability, high leaf protein content and stem fiber content in ZJGS make it both ecological benefits in the mining area and economically valuable. This study aims to further understand ZJGS and other Moraceae taxa through the ZJGS chloroplast (cp) genome structure and the comparison with 12 closely related Moraceae species. Among the 13 Moraceae species, the cp genome length of seven Broussonetia species (ranges from 160,239 bp to 162,594 bp) is larger than that of six Morus species (ranges from 158,459 bp to 159,265 bp). Among the 77 shared protein-coding genes (PCGs) in Moraceae species, the obvious positive selection of Ka/Ks ratios acted on petD and rpl16 genes of B. kazinoki and B. papyrifera, respectively. Phylogenetic analysis based on shared PCGs from 28 species shows that ZJGS is closely related to maternal B. kazinoki. These findings provide data support for the origin of ZJGS hybridization and provide genomic resources for future ZJGS resource development and molecular breeding. 相似文献
7.
The emergence and spread of antibiotic resistance genes among Bacteria are a serious threat to global
health. Their occurrence in animals which are in contact with humans is also important. The Chinese cobra (Naja atra, Elapidae), though a highly venomous species, is appreciated as food and as a source of materials used in traditional Chinese medicine. We are here reporting the isolation of multidrug-resistant Klebsiella pneumoniae (Enterobacteriaceae) from the lung of Naja atra, obtained from a snake farm in a Beijing suburb. Our study analyzed, using gene sequencing, the occurrence of antibiotic resistance genes (ARGs) in three K. pneumoniae isolates from two snakes. In addition, bacterial clones were identified by biochemical tests and phylogenetic analysis. Tests of
antimicrobial susceptibility showed that all K. pneumoniae isolates were resistant to a host of antibiotics (piperacillin, cefazolin, gentamicin, tetracycline, doxycyclin, ciprofloxacin, levofloxacin, lomefloxacin, ofloxacin, norfloxacin, nalidixic acid, chloramphenicol, nitrofurantoin, sulfamethoxazole, and sulfamethoxazole/trimethoprim) but were susceptible to cefotaxime, cefixime, aztreonam, bramycin, amikacin, kanamycin, netilmicin, and streptomycin. Eighteen ARGs were detected in total DNA extracted from the isolates. Results showed three quinolone resistance
genes (oqxA, oqxB, qnrB), the gyrA gene that confers resistance to beta-lactam antibiotics, and the emerging aac(3)-II gene that confers resistance to aminoglycosides. K. pneumoniae is an important opportunistic human pathogen and the emergence of multidrug-resistant K. pneumoniae in N. atra suggests the increasing risk of pathogen transmission
between humans, livestock, and wildlife. Given the close association between foodborne pathogenic microorganisms
and humans, it is key factor to identify these antibiotic resistance genes profile thereby minimize the risk of K. pneumoniae transmission. 相似文献
8.
Plant U-box (PUB) E3 ubiquitin ligases play important roles in hormone signaling pathways and in response to different abiotic stresses, but little is known about U-box genes in Danshen (root of Salvia miltiorrhiza Bunge). Here, we identified and characterized 70 SmPUB genes based on its genome sequence. Phylogenetic analysis of U-box genes from S. miltiorrhiza and Arabidopsis suggested that they can be clustered into seven subgroups (I–VII). Typical U-box domains were found in all identified SmPUB genes through the analysis of conserved motifs. Moreover, qRT-PCR was applied to analyze the relative expression levels of U-box genes in S. miltiorrhiza roots and leaves under PEG-induced water deficit and salt stresses. Results revealed that the SmPUB genes exhibited stronger response to drought than to salt stress. To the best of our knowledge, this report is the first to perform genome-wide identification and analysis of the U-box gene family in S. miltiorrhiza, and the results provide valuable information for better understanding of the function of U-box in S. miltiorrhiza. 相似文献
9.
Background: Serratia ureilytica DW2 is a highly efficient phosphate-solubilizing bacteria isolated from
Codonopsis pilosula rhizosphere soil that can promote the growth of C. pilosula; nonetheless, until now, no validated
reference genes from the genus Serratia have been reported that can be used for the normalization of quantitative
real-time polymerase chain reaction (RT–qPCR) data. Methods: To screen stable reference genes of S. ureilytica
DW2, the expression of its eight candidate reference genes (16S rRNA, ftsZ, ftsA, mreB, recA, slyD, thiC, and zipA)
under different treatment conditions (pH, temperature, culture time, and salt content) was assayed by RT–qPCR. The
expression stability of these genes was analyzed using different algorithms (geNorm, NormFinder, and BestKeeper).
To verify the reliability of the data, the expression of the glucose dehydrogenase (gdh) gene under different soluble
phosphate levels was quantified using the most stably expressed reference gene. Results: The results showed that the
zipA and 16S rRNA genes were the most stable reference genes, and the least stable genes were thiC and recA. The
expression of gdh was consistent with the phosphate solubilization ability on plates containing the National Botanical
Research Institute phosphate growth medium. Conclusion: Therefore, this study provides a stable and reliable
reference gene of Serratia for the accurate quantification of functional gene expression in future studies. 相似文献
10.
Yao‐Li Li Ming‐Ying Shang Ji‐Jun Chen Shao‐Qing Cai 《Microscopy research and technique》2013,76(3):296-310
Many Swertia species are utilized as a traditional medicine under the name “Qingyedan” in China, but are easily confused with one another. To distinguish eight Swertia species (S. mileensis, S. cincta, S. patens, S. punicea, S. delavayi, S. nervosa, S. macrosperma, and S. yunnanensis) and to ensure their safety and efficacy, the microscopic and macroscopic characteristics of the roots, stems, leaves, and flowers of them were examined. The results showed that microscopic and macroscopic features helpful for authentication of the eight species were the sinuosity of the anticlinal walls of epidermal cells and presence or absence of hairs on the leaf lamina; presence or absence of V‐shaped fibers and fibers with sinuous abaxial wall in the sepals; shape of epidermal cells and pattern of papillae on hairs on the margin of corolla nectary; distribution of stomata in leaf and sepal epidermises, stone cells in cortex and phloem of roots and in cortex and pith of stems, crystals in parenchymatous cells of mesophyll and stem, stomata size, stem diameter, and 4‐ or 5‐merous flowers, and so on. Two keys to the eight Swertia species based on macroscopic and microscopic characteristics are presented. The study indicates that microscopy and related techniques are convenient, practicable, and can be unambiguously applied for authentication of Swertia species. Microsc. Res. Tech. 76:296–310, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
11.
To investigate the effects of arbuscular mycorrhizal (AM) fungi on the growth and saline–alkaline tolerance of
Potentilla anserina L., the seedlings were inoculated with Claroideoglomus etunicatum (W.N. Becker & Gerd.) C. Walker &
A. Schüßler in pot cultivation. After 90 days of culture, saline–alkaline stress was induced with NaCl and NaHCO3
solution according to the main salt components in saline–alkaline soils. Based on the physiological response of P. anserina
to the stress in the preliminary experiment, the solution concentrations of 0 mmol/L, 75 mmol/L, 150 mmol/L, 225 mmol/L
and 300 mmol/L were treated with stress for 10 days, respectively. The mycorrhizal colonization rate, mycorrhizal
dependence, chlorophyll content, malondialdehyde content, antioxidant enzyme activities, osmoregulation substances
content and water status were measured. The results showed that with the increase of NaCl and NaHCO3 stress
concentration, mycorrhizal colonization rate, colonization intensity, arbuscular abundance and vesicle abundance
decreased, and reached the lowest value at 300 mmol/L. Strong mycorrhizal dependence was observed after the symbiosis
with AM fungus, and the dependence was higher under NaHCO3 treatment. Under NaCl and NaHCO3 stress, inoculation
with AM fungus could increase chlorophyll content, decrease malondialdehyde content, increase activities of superoxide
dismutase, peroxidase and catalase, increase contents of proline, soluble sugar and soluble protein, increase tissue relative
water content and decrease water saturation deficit. It was concluded that salt–alkali stress inhibited the colonization of
AM fungus, but the mycorrhiza still played a positive role in maintaining the normal growth of plants under salt–alkali stress. 相似文献
12.
The genus Opuntia is a valuable forage resource in arid and semiarid lands during periods of drought and shortage of herbaceous plants. However, absolute minimum temperatures in the plains of Mendoza represent a limiting factor to cultivate several species.
Opuntia ellisiana is a cold hardy species, so the goals of this study were to massively propagate it using in vitro culture techniques, and then to acclimatize plantlets obtained to field conditions.
Different sterilization protocols were tested. Areoles were isolated in laminar airflow cabinet, and cultured on Murashige-Skoog medium, supplemented with sucrose and different BAP and IBA combinations. Explants were grown at 27±2ºC, under a 16-h photoperiod. The shoots produced were used in the rooting assay using different auxin combinations. In the most efficient growth treatment, plantlets reached 100% shooting after 35 days of culture, and a mean length of 10.2 mm after 49 days of culture. A 100% rooted plantlets was obtained on a medium containing 5 mg L-1 IBA, after 12 days of culture. Acclimatization was achieved under greenhouse conditions, showing 100% plantlet survival.
This study suggests that O. ellisiana can be successfully micropropagated by areoles, and easily acclimatizated to field conditions. 相似文献
Opuntia ellisiana is a cold hardy species, so the goals of this study were to massively propagate it using in vitro culture techniques, and then to acclimatize plantlets obtained to field conditions.
Different sterilization protocols were tested. Areoles were isolated in laminar airflow cabinet, and cultured on Murashige-Skoog medium, supplemented with sucrose and different BAP and IBA combinations. Explants were grown at 27±2ºC, under a 16-h photoperiod. The shoots produced were used in the rooting assay using different auxin combinations. In the most efficient growth treatment, plantlets reached 100% shooting after 35 days of culture, and a mean length of 10.2 mm after 49 days of culture. A 100% rooted plantlets was obtained on a medium containing 5 mg L-1 IBA, after 12 days of culture. Acclimatization was achieved under greenhouse conditions, showing 100% plantlet survival.
This study suggests that O. ellisiana can be successfully micropropagated by areoles, and easily acclimatizated to field conditions. 相似文献
13.
Clubroot of Chinese cabbage (Brassica rapa L. ssp. pekinensis), caused by the obligate parasite Plasmodiophora
brassicae, accounts for serious yield losses. The aim of our study was to explore the phytohormone levels and metabolome
changes in the roots of resistant and susceptible B. rapa genotypes at a late stage of infection, i.e., 28 days post-infection.
Both genotypes showed decreased auxin levels after P. brassicae infection except for indole-3-acetic acid. Overall, the
susceptible genotype had higher auxin and cytokinin levels after infection, with the exception of trans-zeatin and 3-
indolebutyric acid as compared to the resistant genotype. Jasmonic acid levels declined after infection regardless of the
genotype. Resistance against clubroot was evident with the increased levels of salicylic acid in the resistant genotype.
The susceptible genotype had a higher number of differentially accumulated metabolites (DAMs) (262) than the
resistant genotype (238) after infection. Interestingly, 132 DAMs were commonly detected in both genotypes when
infected with the pathogen, belonging to metabolite classes such as phenolic acids, amino acids, and derivatives,
glucosinolates, organic acids, flavonoids, nucleotides and derivatives, and fatty acids. The differential metabolite
analysis revealed that metabolites related to amino acid biosynthesis, fatty acid biosynthesis and elongation,
glutathione metabolism, and glucosinolate metabolism were highly accumulated in the resistant genotype, suggesting
their essential roles in resistance against P. brassicae infection. 相似文献
14.
CARMINDA DA CRUZ-LANDIM FÁBIO CAMARGO ABDALLA MARIA ALICE DA CRUZ-HÖFLING 《Biocell》2003,27(3):319-328
The spermatogenesis of Piaractus mesopotamicus was investigated under light and transmission electron microscopy. The specimens were captured from their natural environment (Rio Miranda and Rio Aquidauana, Pantanal Matogrossense, Brazil) during April and September. The results were compared with the spermatogenic data of specimens under captivity condition. In both conditions, P. mesopotamicus presented the typical spermatogenesis pattern of the teleost fishes, showing no significative differences. The spermatozoon was classified as type I, which has a globular head without acrosome, a short middle piece and a long tail constituted only by the flagellum. This type of spermatozoon is considered the basic type in fishes. 相似文献
15.
MD. REZWAN MOLLA MD. MOTIAR ROHMAN MD. ROBYUL ISLAM MIRZA HASANUZZAMAN LUTFUL HASSAN 《Biocell》2022,46(7):1613-1627
Waterlogging is an illustrious abiotic stress and the constrictions it enforces on plant roots have negative effects
on growth and development. This study was undertaken to investigate waterlogging stress tolerant potential in chilli
(Capsicum annum L.) genotypes through evaluating morphological, physiological, biochemical and anatomical
parameters. Thirty-five days old seedlings of 10 chilli genotypes were exposed to waterlogging stress maintaining
water height 3–5 cm over the soil surface artificially for three days. This duration (36–38 DAE) was termed as
waterlogging period, and subsequent withdrawal of waterlogging condition (39–45 DAE) was regarded as a recovery
phase. Based on their survival performance, two tolerant genotypes viz., SRC-517 and BARI morich-2 and two
susceptible genotypes viz., AHM-206 and RI-1(6) were selected for studying stress tolerance mechanism. Under
waterlogging, however, both genotypes (tolerant and susceptible) exhibited reduced root shoot length, dry weight
ratio, petiole weight and leaf area, and noticeable reduction regarding these parameters was observed in susceptible
genotypes. Moreover, tolerant genotypes displayed a higher recovery than susceptible genotypes after removal of
waterlogging stress. Lower reduction of leaf area and photosynthetic pigments as well as higher reduction of relative
water content (RWC) were noticed in susceptible genotypes. Higher accumulation of proline and total antioxidant
capacity (TAC) during waterlogging condition in tolerant genotypes suggested lower oxidative damage. Although both
genotypes lost total soluble sugar (TSS) relative to control at waterlogging stress, better performance was recorded in
tolerant genotypes. During the period after the removal of extra water, a similar genotypic response in terms of TSS
gain was seen. Undoubtedly, under flooding conditions, the development of aerenchyma cells in tolerant genotypes is
a means of tolerance mechanism for long-term survival. Thus, the morpho-physiological and biochemical changes
help to understand the tolerance mechanism in chilli under waterlogging stress. 相似文献
16.
The genus Ziziphora L. (Lamiaceae) is represented by five species (nine taxa) in the Turkish Flora. These taxa are Z. clinopodioides Lam. (subsp. elbursensis, subsp. filicaulis, subsp. kurdica, subsp. rigida), Z. capitata L., Z. persica Bunge, Z. tenuior L., Z. taurica Bieb. subsp. taurica, and Z. taurica Bieb. subsp. cleonioides (Boiss.) Davis which to be an endemic taxon for Turkey. They are strongly aromatic herbs which contain rich pulegone and used as herbal teas and spices and for this reason. In this study, comparative anatomy of the genus Ziziphora growing in Turkey is presented for the first time. In anatomical studies, cross sections of vegetative organs such as the root, stem, and leaf (lamina and petiole) were examined. In addition, to exhibit stomatal distribution and anatomy on adaxial and abaxial leaves were taken surface sections of the lamina and calculated stomatal index. Lamina and petiole anatomy were shown to be of great importance in the taxonomy of the Ziziphora taxa. The presence or absence of sclerenchyma in midrib of lamina and petiole, cortex parenchyma layer, mesophyll structure, and epidermal surface were found to be important characters for identification of Ziziphora taxa. 相似文献
17.
Salinization has led to the deterioration of the ecological environment, affected the growth of plants, and hindered the development of agriculture and forestry. Arbuscular mycorrhizal (AM) fungi, as important soil microorganisms, play significant physiological and ecological roles in promoting plant nutrient absorption and improving soil structure. Puccinellia tenuiflora (Turcz.) Scribn. et Merr. in Songnen saline-alkaline grassland was selected as the research object to observe AM fungal colonization of the roots and explore the species and diversity of AM fungi in symbiotic association with P. tenuiflora. This study showed that AM fungi colonized in P. tenuiflora roots and formed a typical Arum–type mycorrhizal structure. A significant correlation was observed between vesicular abundance and the colonization intensity of mycorrhiza. Isolation and identification revealed 40 species of AM fungi in the rhizosphere of P. tenuiflora, belonging to 14 genera, of which two species could not be identified. The richness of the genus Glomus was the highest, accounting for 30% of the total species. Funneliformis mosseae and Rhizophagus intraradices were isolated from all the samples and were the species with the widest distribution in the rhizosphere of P. tenuiflora. Correlation analysis showed that pH only had a significant impact on the distribution of a few species, such as Glomus pustulatum, Diversispora spurca, Glomus aggregatum, Rhizophagus clarum, and Acaulospora foveata. The present study provides a theoretical basis for further exploring the resources of AM fungi in saline-alkaline soil. 相似文献
18.
Ana Laura CIMADOR Emeli Luciana GALANTE Lucila Ibel MUÑOZ Patricia Silvia ROMANO Antonella Denisse LOSINNO María Cristina VANRELL 《Biocell》2019,43(1):13-20
Trypanosoma rangeli and T. cruzi are both parasitic unicellular species that infect humans. Unlike T. cruzi,
the causative agent of Chagas disease, T. rangeli is an infective and non-pathogenic parasite for humans, but pathogenic
for vectors from the Rhodnius genus. Because both species can coexist in different hosts and overlap their infective
cycles but very little is known about the infection of T. rangeli in mammalian cells, we decided to characterize both the
development of this parasite in cell culture and the effect of therapeutic agents with potential trypanocidal action on
it. We found that T. rangeli exhibits a cycle of infection in Vero cells similar to that for T. cruzi and that the repurposed
drug, 17-AAG, and the natural extract Artemisia sp. essential oil produce a toxic effect on epimastigotes showing
a trypanocidal action from the fifth day of culture. Both treatments also affected the infection of trypomastigotes
and reduced the capacity of replication of amastigotes of T. rangeli. Since T. cruzi / T. rangeli coinfection cases have
been reported, the finding of drugs with potential activity against both species could be significant in the future.
Furthermore, studies of susceptibility of both species to drugs could also help to know the different mechanisms of
pathogenicity in humans displayed by T. cruzi that are absent in T. rangeli 相似文献
19.
We investigated the diversity and composition of microflora in feces of Lycopus lucidus Turcz.-fed mice. In
addition, we evaluated the production of major cytokines (Interleukin-6 and -10) which are related to inflammation
and fatty acid composition of several tissues. 16S ribosomal DNA sequencing-based microbiome taxonomic profiling
analysis was performed utilizing the EzBioCloud data base. Male mice fed on L. lucidus showed a significantly
reduced number of lactic acid bacteria and coliform in the feces compared with the control group (p < 0.05). 16S
rDNA sequencing analysis of fecal samples showed that L. lucidus supplementation decreased the community of
harmful microflora (Enterobacteriaceae including Escherichia coli and Bacteroides sp.) in feces compared with the
control group (p < 0.05). There were no significant differences in mRNA expression of cytokine IL-6 and IL-10
between the control and L. lucidus fed groups. The fecal fatty acid composition in the L. lucidus group had
percentages of 4:0, 6:0, 8:0 and 10:0 in the intestine but those short chain fatty acids were not detected in the control
group. Our results showed that L. lucidus supplementation influenced gut environment by decreasing harmful
microflora and increased the percentages of several short fatty acids. 相似文献
20.
Giselle de Moura CARPES Viviane Dal-Souto FRESCURA Solange Bosio TEDESCO Antonio Carlos Ferreira DA SILVA Thais Scotti do CANTO-DOROW 《Biocell》2014,38(1):7-10
Field collected roots of four populations of Sida rhombifolia were used for preparing aqueous decoctions at two concentrations: 4g/L; and 16g/L. Afterwards, we used three groups of six onion (Allium cepa)
bulbs for testing each population. Slides were made with all bulbs through the smashing technique. Cells in all
phases of the cell cycle of A. cepa were analyzed. The mitotic index (% of cells in mitosis) was calculated, and
the statistical analysis through the χ2 test was carried out at 5% probability. The results showed that the aqueous
extracts of S. rhombifolia have antiproliferative activity at high concentrations. Practically no chromosomal aberrations were induced by treatments. 相似文献