乳制品中纳他霉素的测定

建立了测定乳制品中纳他霉素含量的高效液相色谱法。样品用甲醇萃取后进样,采用Thermo ODS-2250min×4.6mm色谱柱分离,流动相为乙酸铵与氯化铵的缓冲液：乙腈=4：1（体积比）,加入5InL四氢呋喃（每升）,紫外检测;A-R303nm,RSD为0．56％-0．82％（n=5）,标准曲线r=0．9998,平均回收率分别为98．2％,最低检出限为0．05mg／L。本法具有操作简单、灵敏度高和准确性好等优点。     

高效液相色谱法测定糖蜜中的甜菜碱含量

建立了一种简单、快速测定甜菜糖蜜中甜菜碱的方法,即样品经去离子水提取,碱式醋酸铅脱色后,采用高效液相色谱-DAD检测器测定甜菜碱的含量。色谱柱为AgilentZorbaxNH2柱（Φ4．6mm×150mm,5Ixm）,流动相为0．05mol／L KH2PO4溶液（pH=4．53）,流速0．7mL／min,柱温30℃,检测波长195nm。试验结果表明：甜菜碱在0．1mg／mL～5．0mg／mL范围内线性良好,相关系数为0．998;加标回收率为99．5％～99．7％,相对标准偏差（RSD）为0．09％-1．06％（n=3）。     

采用高效液相色谱法测定猪肉中的呈味核苷酸

本文建立了猪肉中呈味核苷酸肌苷酸和乌苷酸的高效液相色谱检测方法。以甲醇．0．05mol／L磷酸二氢钠水溶液为流动相,用ZOBAX-NH2色谱柱分离,二极管阵列检测器检测,检测波长为260nm。该方法线性范围为1．00～100mg／mL,变异系数为0．5%-3％,加标平均回收率为85％以上,方法准确可靠,精密度较高。     

高效液相色谱法测定圣女果中维生素C的含量

建立了一种高效液相色谱测定圣女果中维生素C含量的方法。色谱中采用Hypersil-C18色谱柱,0．1％草酸作流动相,流速1．0mL／min,紫外检测器,检测波长254nm。维生素C在20-100mg／L内有良好的线性关系,线性回归方程为y=51．59x-55．12,相关系数R2=0．9998;5,回收率为97．88％-101．52％,相对标准偏差等于0．15％。所用测定方法简便、快速,能准确测定圣女果中维生素C的含量。     

酱油中Monacolin K的高效液相色谱分析

采用Polaris C18色谱柱（100×2．0mmid,3μm）,水（含0．5％H3PO4）、乙腈为流动相,梯度洗脱,流速0．20mL／min,柱N500c,测定波长（λ）237nm,分析了酱油中MonacolinK,结果显示,MonacolinK在0．2mg/L-5mg／L时显线性关系,加样平均回收率为90．7％,相对标准偏差0．93％-2．7％。     

高效液相色谱法检测小麦粉中过氧化苯甲酰的含量

小麦粉中的过氧化苯甲酰用无水乙醇提取，被碘化钾还原成苯甲酸根，然后用高效液相色谱仪进行测定。采用C18色谱柱，以甲醇-0．02mol／L醋酸铵为流动相，流速1．0mL／min，检测波长230nm。以保留时间定性，以峰面积定量，测定样品中过氧化苯甲酰的含量。结果显示在0～100mg／L范围内线性关系良好，相关系数为0．9996，检测极限为0．1mg／L，相对标准偏差为0．7％．回收率为90．0％～102．5％。试验证明，该方法简便、快捷、准确，用于小麦粉中过氧化苯甲酰含量的检测，取得了满意的结果。     

反相高效液相色谱法测定葡萄中的有机酸

本研究建立了反相高效液相色谱检测葡萄果实有机酸的方法,并评价该方法的准确性和灵敏度。结果显示,用酸性水溶液提取果实有机酸,当应用色谱柱为Kromasil C18,流动相为3％CH3OH-0．01mol／LKH2PO4（pH2．8）缓冲液,流速为0．8mL／min,柱温为25℃,检测波长为210nm时,在8min内可将提取液中的酒石酸、苹果酸、柠檬酸和草酸有效分离。此方法的平均回收率〉90％,定量相对标准偏差均在5％以内,最低检测限在0．067～1．12g／L,表明此法具有快速、灵敏、可靠的特点,能够满足果实样品4种有机酸同时检测的要求。运用该方法,分析了赤霞珠和霞多丽果实发育过程中有机酸含量的变化,发现红、白两品种间有机酸变化趋势相似,即草酸和酒石酸含量逐渐下降,而苹果酸在转色前逐渐积累,而后迅速下降;柠檬酸含量变化不明显。     

聚酰胺固相萃取法在食品安赛蜜和糖精钠检测中的应用

样品用水提取,提取液用聚酰胺小柱富集和净化,用0．02mol／L乙酸铵／甲醇梯度洗脱,色谱柱为XB-C18,5μm,4．6×150mm,波长230nm检测,外标法定量。与国标方法比较,用固相柱萃取可以提高选择性和检测限,检测限可以达到0．2mg／kg。此方法的回收率为87．1％-99．9％,相对标准偏差〈5％（n=6）．在0．02—0．20mg／mL的之间有较好的线性关系．     

高效液相色谱法测定鸡精中呈昧核苷酸含量

建立了高效液相色谱法测定鸡精中呈味核苷酸含量方法。样品经水超声提取。采用液相色谱仪进行检测,色谱柱为C18色谱柱,流动相为V（甲醇）：V（乙酸铵溶液）=5：95,检测波长为254nm。结果表明,鸟苷酸二钠和肌苷酸二钠分离效果良好（R=2．O）,可分别准确定量。在1μg／ml-100μg／ml范围内鸟苷酸二钠和肌苷酸二钠回归方程分别为：Y=10．6825X＋1．5953,r=0．99995;Y=8．9096X＋0．13404,r=0．99997。方法回收率为99．1％、98．7％。     

反相高效液相色谱法测定乳制品中核苷酸方法的研究

建立了高效液相色谱法测定乳制品中核苷酸含量的方法。采用紫外检测器，波长为254nm；色谱柱为迪马钻石C18，柱温为25℃，流动相为①0．01mol／L K2HPO4960ml 0．1mol／L K2HPO440mllpH5；测定腺嘌呤核苷(AMP)、鸟嘌呤核苷(GMP)、次黄嘌呤核苷(IMP)。②乙腈：(0．01mol／LKH2PO4 0．005mol／LK2HPO4 1mmol／L四丁基溴化氨，磷酸调pH5．0)=5：95，测定胞嘧啶核苷(CMP)、脲嘧啶核苷(CMP)。流速为1．0ml／min。五种核苷酸组分。标准曲线相关系数r为CMP0．9868；UMP0．9879；GMP0．9792；IMP0．9799；AMP0．9553；回收率为94．6％；8次样品重复测定变异系数为1．79％。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press     

INFOPRINT 2014 was Successfully Held in Dongguan

Sponsored by Printing and Printing Equipment Industries Association of China （PEIAC） and organized by Print China magazine, the Seventeenth Beijing International Printing Information Conference （INFOPRINT 2014） was successfully held on 11th Dec. 2014 at Dongguan Exhibition International Hotel.