大豆、牛乳发酵制作大豆酸奶的工艺研究

为更好地开发大豆酸奶,介绍了大豆酸奶的生产工艺,详细介绍了原材料的处理、混合浆定容、高压均质、杀菌、冷却、添加生产发酵剂、接种、塑料杯消毒、灌装、主发酵和产品检验等操作要点,认为大豆酸奶的发酵条件:接种量为3%、白砂糖加入量为12%、发酵时间为4 h和发酵温度为42℃。     

凝固型大豆酸奶的加工工艺研究

以大豆蛋白和豆油为主要原料,以感官评定、pH值和持水率为评价指标,对大豆酸奶发酵剂及发酵条件进行了优化。结果表明：发酵剂乳酸乳球菌1号、2号和3号菌种的最佳配比为5：3：2;糖的添加量分别为：蔗糖5％、葡萄糖2％和果糖3％;最适接种量为2．5％,发酵温度为30℃。在上述条件下发酵所得产品凝固性强,豆清析出很少,而且口感细腻、润滑,酸甜适中,并且豆腥味小,含有豆酸奶特有的香味。     

益生菌酸奶的工艺研究

以保加利亚乳杆菌(Lb)、嗜热链球菌(St)、干酪乳杆菌(Lc)、罗依氏乳杆菌(Lr)混合菌发酵,得到益生菌酸奶.正交结果表明,干酪乳杆菌、罗依氏乳杆菌可以应用于酸奶发酵,发酵工艺为:保加利亚乳杆菌:嗜热链球菌:干酪乳杆菌:罗依氏乳杆菌为3:3:2:2,发酵温度39℃,接种量0.25%(DVS),加糖量8%,凝乳时间5h.     

益生菌酸奶的工艺研究

以保加利亚乳杆菌(Lb)、嗜热链球菌(St)、干酪乳杆菌(Lc)、罗依氏乳杆菌(Lr)混合菌发酵,得到益生菌酸奶。正交结果表明,干酪乳杆菌、罗依氏乳杆菌可以应用于酸奶发酵,发酵工艺为:保加利亚乳杆菌∶嗜热链球菌∶干酪乳杆菌∶罗依氏乳杆菌为3∶3∶2∶2,发酵温度39℃,接种量0.25%(DVS),加糖量8%,凝乳时间5h。      

大豆酸奶

研究了以鲜乳和大豆为原料生产大豆酸奶的最适宜配方及生产条件。结果表明,将豆乳与鲜乳以3：2的质量比混合,加入8％的白砂糖、0．2％的乳化稳定剂,选用保加利亚乳杆菌和嗜热链球菌以1：1的接种量,在45℃下发酵4h,可得到优质的大豆酸奶。     

均匀设计法改良双歧杆菌大豆酸奶发酵工艺

采用变温发酵工艺提高利用乳酸菌系和双歧杆菌发酵大豆所制备的酸奶中的活菌数量。采用平行试验法确定大豆干粉和果葡糖浆的用量,利用计算机分析,考察程序变温和发酵时间对大豆酸奶活菌数的影响,并用均匀设计法,对发酵工艺进行优化,得到最佳工艺条件为大豆干粉5.3%,果葡糖浆4%,乳酸菌系(保加利亚乳杆菌∶嗜热链球菌=1∶1)接种量0.03%(以菌种干粉/总固形物计),双歧杆菌接种量0.15%(以菌种干粉/总固形物计),第1阶段发酵为温度42℃,发酵2.5 h,第2阶段为温度36℃,发酵3 h,终产品中活菌数达到6.5×106个/mL以上。     

绿豆酸奶发酵工艺的研究

本文研究了绿豆酸奶的发酵工艺。结果表明,嗜热链球菌与植物乳杆菌可应用于绿豆酸奶的发酵,采用正交试验,确定绿豆酸奶的最佳发酵工艺为:嗜热链球菌与植物乳杆菌比例为2:1,接种量为3%,发酵温度为41℃,发酵时间为5h。     

新型大豆酸奶的研究

本研究以大豆为主要原料，经过膨化处理，将保加利亚乳杆菌(L．bulgaricus)和嗜热乳酸链球菌(Str．themophilus)混合作为发酵剂进行乳酸发酵。用L9(3^4)正交实验得出最佳工艺条件，研制出了具有浓郁风味的乳酸菌发酵酸奶。     

单一干酪乳杆菌KL1发酵益生菌酸奶工艺条件的研究

应用单因素实验及正交实验,确定单一干酪乳杆菌KL1发酵酸奶的最佳原料乳,并对其益生菌酸奶发酵工艺条件进行优化。筛选得到的最佳原料乳为M₂,同时通过添加M₆改善酸奶的色泽与组织状态;采用三因素三水平L₉（3⁴）正交实验优化酸奶的发酵工艺条件,接种量是影响KL1发酵酸奶品质的最主要因素,确定干酪乳杆菌KL1发酵酸奶的优化条件如下:接种量为6.0%,M₆添加量1.0%,葡萄糖添加量1.5%,37℃下发酵酸奶22 h,优化后的益生菌酸奶的活菌数量较对照组高10倍。      

大豆酸奶加工工艺研究

从提高豆浆附加值及应用研究的角度,研究了大豆酸奶的加工工艺.通过单因素以及正交实验的设计方法,确定了大豆酸奶加工过程中辅料添加量、均质、发酵等重要参数.分析数据为大豆酸奶的工业化生产提供了重要理论依据.     

混合发酵法生产花粉面包的工艺优化

玉米花粉营养丰富。制作面包时,在面团中加入破壁的玉米花粉。将酵母菌与乳酸菌以一定的比例混合,作为发酵剂。经过发酵和焙烤,研制出具有花粉清香的保健型花粉面包。混合发酵法生产花粉面包的最佳工艺条件是:玉米花粉添加量为5%,糖添加量为10%,酵母菌与乳酸菌配比为2.5∶1,发酵时间为5h。     

Production of free conjugated linoleic acid by Lactobacillus acidophilus and Lactobacillus casei of human intestinal origin

A gas chromatographic procedure was used for analysis of conjugated linoleic acid (CLA) isomers cis-9, trans-11-octadecadienoic; trans-10, cis-12 octadecadienoic; and trans-9, trans-11-octadecadienoic (c9t11, t10c12, t9t11) produced by lactobacilli. Four different cultures, two strains each of Lactobacillus acidophilus and Lactobacillus casei were tested for their ability to produce CLA from free linoleic acid in MRS broth supplemented with linoleic acid. Different concentrations of linoleic acid (0, 0.05, 0.1, 0.2 and 0.5 mg/ml) were added to MRS broth, inoculated with the lactobacilli, and incubated at 37 degrees C. Viable counts and amounts of individual isomers of CLA (c9t11, t10c12, t9t11) were measured at 0, 24, 48, and 72 h. All the cultures were able to produce free CLA in media supplemented with linoleic acid. Maximum production of CLA (80.14 to 131.63 microg/ml) was observed at 24 h of incubation in broth containing 0.02% of free linoleic acid. No significant (P > 0.05) increases in total CLA levels were observed after 24 h of incubation. The ability of the cultures to produce CLA in skim milk supplemented with 0.02% free linoleic acid also was studied. In this medium, the total amounts of free CLA after 24 h of incubation ranged from 54.31 to 116.53 microg/ml. The use of lactic acid bacteria able to form free CLA in cultured dairy products may have potential health or nutritional benefits. Free CLA in the products likely would be more readily available for absorption from the digestive tract than if it were incorporated into the cells of the starter culture.     

发酵肉制品中益生性乳酸菌的快速筛选

利用一种快速的分离筛选法,即利用pH 2.5和1% 胆盐的MRS液体培养基,对自然发酵肉制品中耐高酸耐胆盐的乳杆菌进行富集,从中分离纯化得到77株乳杆菌.通过对分离菌株进行耐高酸(pH值2.5)、0.3%胆盐的生长的进一步测定,筛选出了21株耐酸及胆汁酸盐菌株,最后运用API 50CHL对筛选菌株进行了鉴定.这些菌株可活体摄入人体肠道,发挥其益生性,为益生性发酵剂开发奠定了基础.     

嗜酸乳杆菌发酵乳的研究

以嗜酸乳杆菌和嗜热链球菌为发酵剂,发酵制成具有保健功能的新型酸乳——嗜酸乳杆菌发酵乳。     

乳酸菌饮料的稳定性

稳定性是乳酸菌饮料生产和储存过程中经常遇到的问题。本文从配方和制备工艺入手,对影 响乳酸菌饮料稳定性的一些主要因素进行了研究探讨,提出了解决乳酸菌饮料稳定性的有效方法。     

乳酸菌饮料的工艺研究

重点介绍了乳酸菌饮料工艺流程,确定工艺的最佳条件。     

Fermentation by Amylolytic Lactic Acid Bacteria and Consequences for Starch Digestibility of Plantain, Breadfruit, and Sweet Potato Flours

The potential of tropical starchy plants such as plantain (Musa paradisiaca), breadfruit (Artocarpus communis), and sweet potato (Ipomoea batatas) for the development of new fermented foods was investigated by exploiting the capacity of some lactic acid bacteria to hydrolyze starch. The amylolytic lactic acid bacteria (ALAB) Lactobacillus plantarum A6 and Lactobacillus fermentum Ogi E1 were able to change the consistency of thick sticky gelatinized slurries of these starchy fruits and tubers into semiliquid to liquid products. Consequently, a decrease in apparent viscosity and an increase in Bostwick flow were observed. These changes and the production of maltooligosaccharides confirmed starch hydrolysis. Sucrose in sweet potato was not fermented by strain A6 and poorly fermented by strain Ogi E1, suggesting possible inhibition of sucrose fermentation. In all 3 starchy plants, rapidly digestible starch (RDS) was higher than slowly digestible starch (SDS) and resistant starch (RS) represented between 17% and 30% dry matter (DM). The digestibility of plantain was not affected by fermentation, whereas the RDS content of breadfruit and sweet potato decreased and the RS content increased after fermentation. Practical Application: The characteristics resulting from different combinations of gluten free starchy plants (plantain, breadfruit, sweet potato) and amylolytic lactic acid bacteria (ALAB) offer opportunities to develop new functional fermented beverages, mainly for breadfruit and sweet potato, after further investigation of their formulation, sensory attributes, nutritional, and prebiotic characteristics.     

益生菌预防常见老年疾病的机制

老年人常见的老年病如阿尔兹海默症、骨质疏松、心血管疾病等不仅会给患者本人带来痛苦,还会导致一些经济问题.人类肠道中存在10种以上微生物,大多数分布在回肠和结肠,这些细菌在维持能量、代谢和免疫等方面起到重要的作用.本文总结分析了益生菌预防阿尔兹海默症、骨质疏松、心血管疾病等老年疾病的机制以及临床应用,以期为老年人的健康状...     

多菌种发酵生产保健型酸奶的研制

通过对保加利亚乳杆菌、嗜酸乳杆菌、干酪乳杆菌、双歧杆菌等九种乳酸菌进行复配，用正交实验，对多菌株混和发酵条件作了初步探讨，从风味、活菌数数量上确定混和菌种的最佳组合，为生产保健作用更好的新型酸奶打下基础。     

乳酸菌类细菌素的研究进展

对乳酸菌进行了简要分类，然后按乳酸菌产生的细菌素的结构组成特点进行了分类，对不同类型的乳酸菌细菌素的组成特点和功能特点进行了简要介绍。此外，对其合成过程及其产生抗菌活性的可能机理进行了描述，在此基础上，对影响乳酸菌类细菌素抗菌作用效果的外界因素和内部环境条件进行了详细分析。最后，论述了目前乳酸菌类细菌素在食品中的应用现状，对其在食品行业中的应用前景进行了探讨和预测。