PCR-DGGE分析资中冬尖发酵过程中细菌多样性

目的:采用聚合酶链式反应-变性梯度凝胶电泳(polymerase chain reaction-denatured gradient gel electrophoresis,PCR-DGGE)技术分析资中冬尖发酵过程中微生物群落结构及其动态变化。方法:从资中采集4份不同发酵年份冬尖样品,提取样品总DNA,采用巢式PCR法扩增细菌16S r DNA V3区,扩增产物采用DGGE分离,对细菌主要优势条带进行克隆、测序、构建系统发育树。结果:冬尖在发酵过程中细菌多样性较丰富,且群落结构发生了较大变化。不同发酵时间样品间,细菌群落结构相似性为9%~67%,其中第2年与第3年样品相似性最高,达67%。资中冬尖发酵过程中,所涉及的细菌主要分为3类,分别为厚壁菌门(Firmicutes)、变形杆菌门(Proteobacteria)和非培养菌,其中厚壁菌门为优势菌群,占53%;变形杆菌门占37%;非培养菌仅占10%。结论:采用PCR-DGGE技术能更全面、更真实地反映资中冬尖在发酵过程中微生物群落的多样性及其动态变化,为冬尖的生产工艺和风味物质的形成提供理论支撑。     

资中细嫩冬尖

四川资中冬菜创始至今约200多年历史,早在清朝道光年间就是四川有名的腌菜之一。曾远销北京、天津、上海、武汉、广州等地,还曾外销港澳及东南亚国家,在国内外亨有盛誉。资中冬菜系指细嫩冬尖,附产品有二     

资中冬尖和南充冬菜

介绍了资中冬尖和南充冬菜的独特生产工艺，从原材料到加工方法以及两种名特食品的品质标准都作了详细介绍。     

不同年份资中冬尖中香味成分的GC-MS分析

采用同时蒸馏萃取(SDE)提取资中冬尖中的挥发性香味物质,利用气相色谱-质谱联用仪(GC-MS)对其挥发性香味成分进行分离和鉴定确认了23种成分.初腌冬尖中含量最高的是甲醇(0.061％)、2-丁烯(0.044％)、反1,2-二氯乙烯(0.032％)和二甲基硫(0.029％);腌制一年的冬尖中含量最高的是亚麻酸乙酯(0.056％)、甲醇(0.056％)、2-丁烯(0.039％)和反1,2-二氯乙烯(0.033％);两年冬尖中含量最高的是亚麻酸乙酯(0.138％)、2-丁烯(0.039％)、反1,2-二氯乙烯(0.031％)和乙酸(0.030％);特制冬尖中最高含量是二甲基硫(0.09％)、过氯乙烯(0.08％)、十八烷三烯酸乙酯(0.072％)和反1,2-二氯乙烯(0.069％).     

调味副食品供求信息

品牌规格产地单价(元)单位联系人供/求际怕天掷椒育墩450兀/吨毕1义:盐伽m酋脑供swa＋nn、,＿。。联系人:傅加义,u页HW盐城3300元/吨1二“x卫”“二^－＿。供二二二?”?———””“”－“电is,0515—8242603”“甜血酋盐喊250()T/吨地址．建军桥东河边4号供细嫩冬尖凹他Xf四川资中1．89单位:四川资中酿造厂供细嫩冬尖叩吨X40”3．60联系人:朱远红供碎粒细嫩冬尖兀他X64”2．20电话:0832－552219供碎粒细嫩冬尖叩他X40”4．o地址:重龙镇桥北酉路18号供风味豆鼓ZopX24”2．95供风味豆鼓45%X24”5．20供香辣酱凹吨X24”1．80供香辣酱扣地…     

滩羊皮漂白、染色工艺技术研究

论述了滩羊皮漂白、染色工艺技术及操作要点，并针对该工艺控制过程中的影响因素，提出了相应的技术措施。     

峡江生麦制曲的生产工艺初探

“峡江老冬酒”是以当年收割的早尖糯米为原料,并用农家自制的酒曲粒和踩制生麦曲为糖化发酵剂酿制而成。其风味来源主要得益于当地得天独厚的自然条件和以江皮、小麦、红泥土为原料踩制成的生麦曲。该文初探了峡江生麦制曲的工艺制造和操作要求,在此基础上提出了传统生麦踩曲中所含微生物种群和生麦曲的特征和作用。     

新疆特色风味色拉酱的加工工艺

为了生产出高品质具有新疆特色风味的低脂色拉酱,对其基本配方、加工工艺及关键危害控制点进行了论述,确定了新疆特色风味低脂色拉酱的工艺操作技术及关键危害控制点.     

试论油脂浸出厂的技术管理

本文综合论述了技术管理在油脂浸出厂企业管理中的重要地位、工作范围和内容。对工艺管理、设备管理和人才管理等方面分别提出了多项加强措施。工艺管理方面:1.不断深化研究和改进生产工艺;2.制定和执行生产操作规程和操作指标:3.建立和执行检测制度;4.消耗定额管理;5.贯彻国家有关安全防火规范和执行本厂的安全制度和规程。设务管理方面:1.设备选型与革新;2.建立设备档案制度;3.设备维护与检修。人才管理方面;1.开展定期和不定期技术培训活动;2.有组织地开展合理化建议活动;3.制定和执行好技术工人的技术标准。     

高级调味油的生产工艺总结

论述了调味油的加工工艺、加工设备、操作技术、产品质量及产品市场前景。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press