White cell-reduced red cells prepared by filtration: a critical evaluation of current filters and methods for counting residual white cells

Atrial natriuretic peptide (ANP) is reported to dilate a major coronary artery in both experimental animals and humans. Spasm of a major coronary artery is the cause of variant angina pectoris and can be induced by hyperventilation. The effect of the ANP infusion on anginal attack induced by hyperventilation was studied in patients with variant angina pectoris. The study was performed in the early morning on 3 consecutive days in 11 patients with variant angina pectoris in whom the attacks were reproducibly induced by hyperventilation. On days 1 and 3 (saline solution infusion), and day 2 (ANP infusion), hyperventilation was started 14 minutes after beginning infusion of ANP (0.1 microgram/kg/min) or saline solution for 6 minutes. The attacks were induced in all 11 patients by hyperventilation on days 1 and 3. However, the attacks were not induced in any patient on day 2 of the ANP infusion. The plasma ANP level increased from 33 +/- 7 pg/ml to the peak level of 2,973 +/- 479 pg/ml (p < 0.01) at the end of the ANP infusion, and the plasma level of cyclic guanosine monophosphate (cGMP) increased from 5 +/- 1 pmol/ml to the peak level of 58 +/- 6 pmol/ml (p < 0.01) 5 minutes after the ANP infusion. The plasma levels of ANP and cGMP did not change after hyperventilation on days 1 and 3. It is concluded that the ANP infusion suppresses the attacks induced by hyperventilation in patients with variant angina pectoris, and cGMP is related to the mechanisms of suppression of the attacks.     

Quality control of white cell-reduced red cells: white cell preservation and simplified counting

This article reviews recent studies concerning memory and language disorders in Alzheimer's disease and other neurodegenerative conditions. It shows how different memory and language subcomponents may differentially be impaired in different neurodegenerative diseases and at different stages of the same disease.     

Prestorage versus bedside white blood cell filtration of red blood cell concentrates: effects on the content of cytokines and soluble tumor necrosis factor receptors

BACKGROUND: The cytokine network has important implications for the systemic inflammatory and metabolic response in trauma and infection. The objective of this study was to investigate the influence of white blood cell (WBC) filtration on the cytokine content in red blood cell concentrates (RBCs). STUDY DESIGN AND METHODS: Tumor necrosis factor-alpha (TNF), interleukin-1-beta (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), and soluble TNF receptors I and II (sTNF-RI, sTNF-RII) were investigated in filtered and nonfiltered RBCs during storage. After 40 days of storage the originally nonfiltered units were filtered. RESULTS: On day 1 prestorage filtered RBCs had lower concentrations of WBCs (p < 0.001), TNF (p < 0.01), sTNF-RI (p < 0.01) and sTNF-RII (p < 0.05) compared to nonfiltered units. IL-1 concentrations increased from day 1 to day 40 (p < 0.05) in nonfiltered RBCs and were higher in nonfiltered units compared to prestorage filtered ones on day 40 (p < 0.05). An increase of IL-8 was found in nonfiltered RBCs as well as prestorage filtered units from day 1 to day 40 (p < 0.05) but the concentrations of IL-8 were higher in nonfiltered units on day 40 compared to prestorage filtered units (p < 0.05). Filtration at the end of the 40-day storage period had no influence on the concentrations of cytokines and soluble TNF receptors. CONCLUSION: The present results suggest that prestorage WBC filtration may be more efficient in reducing the cytokine content of RBCs compared to filtration at the the end of the storage period. The clinical impact of passive transfer of components of the cytokine network via RBCs, e.g., in critically ill patients, is however unclear and needs further investigations.     

Changes in the white blood cell picture of calves commercially raised for the 1st 30 days after birth

The changes were studied in the white blood picture of calves commercially raised from day 1 to 30 after birth. It was found that eosinopenia sets on following day 3 postpartum and persists to day 22 while aneosinophilia persists to day 11 after birth, this being at the expense of lobocytes. It was further found that when calves are first fed pooled milk neutrophil total number rises temporarily--mostly young neurophils (red nucluar cells to metamyelocytes). It is suggested that this may be due to their release from the depots.     

Effect of 24-hour storage at 25 degrees C on the in vitro storage characteristics of CPDA-1 packed red cells

We have studied 120 patients with EEGs that show defective reactivity of alpha activity in which one side fails to attenuate during the performance of mental arithmetic. The side of defective reactivity was the right side in 74 patients and the left in 46. A similar asymmetric reactivity of alpha activity to eye opening (Bancaud phenomenon) was seen in 32 patients. Also, 113 patients had lateralized EEG abnormalities on the side of defective reactivity and on the side in which cerebral lesions or disorders of cerebral function were present. The unilateral failure of alpha attenuation with mental concentration represents a subtle lateralized electrographic manifestation of cerebral dysfunction on the side of defective reactivity.     

Hydrothermal preparation and photoluminescent property of LiY(MoO4)2:Pr3+red phosphors for white light-emitting diodes

Praseodymium doped lithium yttrium molybdate Li Y1-8x Pr x(Mo O4)2(x=0.005–0.025) phosphors were successfully prepared by the hydrothermal method. The phase, morphology, and luminescent property of the prepared phosphors were investigated by X-ray diffraction and scanning electron microscopy. The results indicated that doping of Pr3+ ions did not change the main phase of the phosphors. The samples emitted red luminescence upon excitation at 453 nm and the strongest emission peak corresponding to the characteristic transition of the Pr3+ ion: 3P0→3F2 was observed at 657 nm. Li Y(Mo O4)2:Pr3+ red phosphors could be effectively excited by blue light emitting-diodes to emit red light; thus, acting as potential candidates for compensating the red light deficiency of cerium doped yttrium aluminum garnet yellow phosphor.     

铜,铅,精矿中金,银分析取制样方法研究成果的应用

介绍了铜，铅精矿中金，银分析取制样方法研究的成果，其中金，银品质波动分类，在企业应用上，有着明显的经济效益和一定的社会效益。     

Effects of SL-probiotic preparation on the body weight and phagocytosis of white mice

Specimens from 15 young patients presenting with acute epididymitis were tested for the presence of Chlamydia trachomatis by an enzyme immunoassay (EIA), polymerase chain reaction (PCR), and for other bacteria by standard laboratory techniques. C. trachomatis urethral infection was detected in 3 patients by an EIA test of the urethral swabs (20%) and in 13 patients by the PCR (87%). This difference in detection rate was statistically significant (p < 0.005). Thirteen specimens were positive by the PCR, but only three of them were positive by the EIA method. These findings indicate that the PCR assay is a highly sensitive assay for the detection of C. trachomatis in male urine specimens and provides a noninvasive technique for routine screening of chlamydia infection in the patient with acute epididymitis.     

Influence of Ostertagia ostertagi and Cooperia infections on the energetic efficiency of steers fed a concentrate ration

Studies on effects of subclinical parasitism (low worm burden) in full-fed and maintenance-fed steers indicated that over a short interval low worm burdens do not have a statistically significant effect on the efficiency of energy utilization. In full-fed steers, energy retention tended to be higher in steers with lower worm burdens (mean 95.8 Ostertagia, 12.5 Cooperia) than in those with higher worm burdens (mean 677.5 Ostertagia, 201.8 Cooperia). Maintenance-fed steers generally had larger numbers of Ostertagia ostertagi and Cooperia spp than did the full-fed steers.     

Relationship between appearance of urinary red blood cell/white blood cell casts and the onset of renal relapse in systemic lupus erythematosus

The purpose of the study was to determine the extent to which urinary sediment findings (changes in red blood cells [RBCs], white blood cells [WBCs], and the appearance of RBC and WBC casts) predict the onset of renal relapse (defined as a specific increase in proteinuria and/or serum creatinine level) in patients with systemic lupus erythematosus (SLE). Seventeen SLE patients with biopsy-proven diffuse proliferative glomerulonephritis at initial presentation were followed prospectively for 1,129 patient-months under a study protocol. Semiquantitative urinalyses were performed at 2-month intervals during periods with little or no SLE activity and, more frequently, during periods with increased SLE activity. Each urinalysis was accompanied by a clinical evaluation and a panel of screening tests relevant to the evaluation of SLE activity. During this study, 877 semiquantitative urinalyses were performed and 43 renal relapses were observed in 14 patients. No relapse occurred in three patients. Of the renal relapses, 30 were defined as proteinuria relapses (mean baseline proteinuria increased from 0.8 +/- 0.1 g/24 hr to 2.7 +/- 0.3 g/24 hr; P < 0.001) and 13 were defined as serum creatinine relapses (mean baseline serum creatinine increased from 2.7 +/- 0.4 mg/dL to 3.8 +/- 0.5 mg/dL; P < 0.001). Red blood cell and/or WBC casts (cellular casts) were observed before or at the onset of 35 of the 43 renal relapses (sensitivity, 81%). The mean and median intervals between the appearance of cellular casts and the onset of renal relapse was 10 +/- 2 weeks and 8 weeks, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)     

华蟾素注射液对人肝癌HepG-2细胞增殖及周期的影响

Objective: The aim of our study was to investigate the effect of Cinobufacini injection on the proliferation and cell cycle of human hepatoma HepG-2 cells. Methods: Cell proliferation was assessed by MTT assay, cell cycle distributionwas detected by the flow cytometry (FCM). The expression of Cyclin A, CDK2 mRNA levels were examined by RT-PCR.Quantitative colorimetric assay was used to analyze Cyclin NCDK2 activity in HepG-2 cells. Results: Cinobufacini injection significantly inhibited HepG-2 cells proliferation in dose- and time-dependent ways; FCM analysis showed Cinobufacini injection induced cell cycle arrest at S phase; RT-PCR assay showed Cinobufacini injection down-regulated Cyclin A, CDK2expression at mRNA levels; Quantitative colorimetric assay showed Cinobufacini injection deceased Cyclin A/CDK2 activity in HepG-2 cells. Conclusion: Cinobufacini injection can inhibit human hepatoma HepG-2 cells growth, induce cell apoptosis and induce cell cycle arrest at S phase, the mechanism of which might be partly related to the down-regulation of Cyclin A,CDK2 mRNA expression and inhibition of Cyclin A/CDK2 activity.     

锌精矿焙烧工艺条件对湿法炼锌总硫利用率及锌浸出率的影响

叙述了锌精矿沸腾焙烧工艺操作条件对湿法炼锌总硫利用率及锌浸出率的影响,介绍了西北铅锌冶炼厂锌精矿沸腾焙烧的生产实践。     

锌精矿焙烧工艺条件对湿法炼锌总硫利用率及锌浸出率的影响

叙述了锌精矿沸腾焙烧工艺操作条件对湿法炼锌总硫利用率及锌浸出率的影响,介绍了西北铅锌冶炼厂锌精矿沸腾焙烧的生产实践.     

Relation between the osmolality trend and ornithynedecarboxylase activity in red blood cells of uremic patients during hemodialytic treatment

We validated the Hypomania Interview Guide-Seasonal Affective Disorder version (HIGH-SAD) in patients with rapid cycling bipolar disorder (RCBD). Fourteen outpatients were rated on six separate occasions (total = 84 visits). On each visit the patients were rated with the HIGH-SAD and the Young Mania Rating Scale (YMRS) in a counterbalanced order. Clinical assessment was completed at the end of the visit by the treating psychiatrist. Patients were assessed as hypomanic/manic on 22 of the visits. Pearson correlation coefficient between the YMRS total scores and the HIGH-SAD total scores for those 22 visits in which patients were hypomanic/manic was r = 0.629 (P < 0.05) and for all visits was r = 0.769 (P < 0.0001). Analysis with only one rating per patient yielded a Pearson correlation coefficient of r = 0.792 (P < 0.0004). We found that the HIGH-SAD was a valid scale for the measurement of hypomania in patients with RCBD. However, the scale does not differentiate hypomania from mania in this group of patients.     

State of the hypophyseal-adrenal system during surgical correction of heart defects under various anesthesiological methods and at distant periods after surgery

The concentration of 11-HOCS in the blood plasma was measured in 278 patients with heart diseases operated on under various anesthesias at different stages of surgical correction of the defects and in the immediate postoperative period. Plasma ACTH level was measured in 137 of these operated on under deep hypothermia and hypothermal perfusion. The hormonal response was the most expressed in patients operated on under normothermia, whereas in patients exposed to hypothermia the reaction was minimal. 141 patients were examined in remote periods after surgery. Blood plasma 11-HOCS level was normal in the cases with good and satisfactory results of surgical treatment, whereas in those with poor results it was higher or lower than normally.