山奈酚的分析方法

药理研究表明山奈酚在抗癌、抗菌等方面具有很大的药用价值。介绍目前测定山奈酚的分析方法,以更好地分离鉴别和检测山奈酚。     

山奈酚分子印迹聚合物的制备及其性能表征

采用分子印迹技术,以山奈酚(Kaempferol,KAE)为模板分子,2-乙烯基吡啶(2-Vpy)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,合成了山奈酚分子印迹聚合物(KAE-MIP)。采用静态平衡结合实验评价了KAE-MIP对底物分子的结合特性,并进行了吸附动力学研究。结果表明,KAE-MIP对KAE呈现出了很高的选择吸附特性;Scatchard分析显示KAE-MIP对KAE存在不同亲和力的两类结合位点;与对照物在KAE-MIP上的吸附行为比较表明,KAE-MIP对KAE具有良好的分子识别性能;KAE-MIP对KAE的选择性吸附基本在2 h之内完成。     

毛细管电泳法测定沙棘药物制品心达康片中的山奈酚和槲皮素

建立了毛细管电泳法测定沙棘药物制品心达康片中山奈酚和槲皮素的方法,研究了缓冲溶液pH和浓度、分离电压和进样时间对分离的影响,通过分析实际样品并做加入回收实验验证了方法的可行性。     

天胡荽中槲皮素与山奈酚的含量测定

目的：采用高效液相色谱法测定天胡荽中槲皮素、山奈酚的含量。方法：采用ODS色谱柱（250 mm×4.6 mm,5μm）,以乙腈和0.4%磷酸为流动相进行梯度洗脱,流速0.8 mL/min,柱温30℃,检测波长为368 nm。结果：天胡荽中槲皮素与山奈酚的线性范围分别为2.51～50.15μg/mL与2.51～50.25μg/mL,相关系数分别为0.999 7与0.999 6;平均加样回收率为99.7%和100.3%。结论：该方法可靠、简便、准确,可作为天胡荽药材中槲皮素、山奈酚的含量测定方法。     

山奈酚-铜配合物的合成、表征及抗氧化活性研究

黄酮类化合物具有多种生物活性,其部分金属配合物的活性会增强.本文研究山奈酚-铜配合物的制备及其生物活性.以山奈酚和新戊酸铜为原料,按照摩尔比2∶1和1∶1进行配位反应,合成山奈酚-铜配合物.通过紫外光谱、红外光谱、元素分析对其结构进行了表征,比较了山奈酚及其铜配合物清除O2-·自由基和DPPH自由基的能力.结果表明山奈...     

对二氟苯的合成研究

对二氟苯是重要的有机合成中间体，主要应用于染料、农药及医药等行业，是有机合成和精细化工的重要原料。本文报道了一条对二氟苯的合成路线。以对氯硝基苯为原料，与氟化钾反应制得对硝基氟苯，在Fe/NH4Cl体系中还原成对氟苯胺，再Schiemann反应得到对二氟苯。     

山奈酚羟丙基-β-环糊精包合物的制备与评价

制备了山奈酚羟丙基-β-环糊精包合物.通过紫外光谱(U V)、红外光谱(IR)、熔点等分析方法对制备的包合物进行物相鉴定;考察其还原能力及在不同pH值条件下的释药性能.结果表明,山奈酚与羟丙基-β-环糊精发生了包合作用并非简单的物理混合.包合物在pH=1和pH=68.的释药曲线符合一级动力学,在pH=1的释放介质中累积...     

对硝基氟苯及对氟苯胺的合成研究

本研究提出氟苯为原料,混酸作硝化剂制备对硝基氟苯的合成路线。实验研究下反应因素如硝酸化、混酸组成、反应温度、反应时间对产品收纺的影响,并确定最侍反应条件。产品的纯度符合要求,产品的收率均达到９０％以上。此外,也提出对氟苯胺的合成方法。     

The relevance of laboratory studies on delayed ettringite formation to DEF in field concretes

Many laboratory studies of delayed ettringite formation (DEF) have been conducted on thin mortar bar specimens, heat treated, and then immersed in water. Under these conditions, rapid diffusion of alkali hydroxide into the surrounding water occurs and necessarily reduces the alkali hydroxide concentration of the mortar pore solution. Results reported recently by Famy indicate that the DEF process is triggered as a consequence of such leaching. When it is prevented by immersion into alkali hydroxide solution instead of water DEF expansion is delayed or prevented entirely. Results reported by Zhang indicate that 51-mm mortar cubes behave differently than more leaching-susceptible mortar bars when exposed to the same wet environment. Mortars that show severe DEF as mortar bars remain almost free of DEF symptoms if they are stored as cubes, even after 900 days. Attention is called to the fact that DEF in concrete is found commonly in thick concrete members where the possibility of leaching is remote. For such concrete, the reduction in internal alkali hydroxide concentration that occurs with ASR can substitute for the effect of leaching. It is postulated that without effective reduction of alkali hydroxide concentration by one or the other process, DEF remains latent.     

Studies on delayed ettringite formation in heat-cured mortars: II. Characteristics of cement that may be susceptible to DEF

Expansions of mortar bars, stored over (but not in) water after simulated steam curing to 85 °C, were related to certain cement compositional parameters. The relationship is expressed in the form of a “delayed ettringite formation (DEF) index.” The DEF index is computed as the joint product of the SO₃/Al₂O₃ molar ratio of the cement, the sum of its SO₃ and Bogue C₃A percentages divided by 10 and the square root of the alkali content expressed as equivalent % Na₂O. The mortars studied were made with 18 different cements, prepared from a set of six representative clinkers by incorporating Terra Alba gypsum to total SO₃ contents that were 1% below optimum, at optimum and 1% above optimum (as defined in ASTM C 563). Measurements of expansion were recorded at intervals for up to 1400 days. Severe cracking and prominent DEF-induced expansions were observed in mortar bars derived from four of the six ‘oversulfated’ cements and lesser expansions from three of the six cements prepared at optimum SO₃ contents. No expansion was found for cements of DEF index below a threshold value; above this value expansions were approximately proportional to the difference between DEF index and its threshold value. The relationship confirms the significance of all three compositional parameters making up the index, e.g., the SO₃/Al₂O₃ molar ratio, the joint contents of SO₃ and C₃A, and the alkali content, in influencing the extent of DEF-induced expansion. In these measurements, the apparent pessimum effect for SO₃ content previously reported by others was not found, although SO₃ contents examined spanned the supposed pessimum value of 4%. Rather, expansion increased with increasing SO₃ content for mortars made with all clinkers exhibiting expansion.     

Studies on delayed ettringite formation in early-age, heat-cured mortars: I. Expansion measurements, changes in dynamic modulus of elasticity, and weight gains

Mortars were prepared from laboratory cements blended from a set of six representative ground clinkers and Terra Alba gypsum. The addition of gypsum was such that cements containing 1% SO₃ less than the optimum SO₃ content, the optimum SO₃ content, and 1% greater than the optimum SO₃ content were produced. Mortar bars and mortar cubes containing each of these cements were exposed to continuous room temperature (23 °C) curing, or to early-age curing cycles involving maximum temperatures of 55 and 85 °C, followed by long-term exposure at 100% RH over water, but not immersed in water. Measurements of expansion, dynamic elastic modulus, and weight gain were recorded at intervals of up to 900 days. Severe cracking and prominent delayed ettringite formation (DEF)-induced expansions were observed in 85 °C cured mortar bars derived from four of the six “oversulfated” cements. Much smaller expansions were observed in mortar bars from two cements with optimum SO₃ content cements also cured at 85 °C. No expansion or other visible indication of distress was observed for any of the 55 °C or continuously room-temperature-cured mortars. The dynamic elastic modulus increased progressively on prolonged exposure for the unaffected mortar bars, but it decreased precipitously after the onset of expansion in affected mortar bars. Significant weight increases also accompanied the processes of expansion. Mortars that showed severe cracking and deterioration when exposed as mortar bars suffered almost no visible damage when exposed as cubes.     

海水淡化能量回收装置用分腔式切换器研究

能量回收装置是降低反渗透海水淡化系统能耗的关键设备,阀控能量回收装置是其中最主要的产品类型之一。针对现有阀控能量回收装置流量和压力波动的问题,本文设计和制造了用于阀控能量回收装置的分腔式流体切换器,并分析了切换器高压腔和低压腔结构设计特点。对安装有此切换器的阀控能量回收装置的流体力学特性进行了实验测试。结果表明,通过设置适宜的切换器高压腔流道开度,可有效实现阀控能量回收装置高压盐水增压原料海水过程中流量供给的连续性和流体压力的平稳性;所研制的分腔式切换器对保证装置运行安全性及降低设备制造成本具有实用价值。     

耐甲氧西林金黄色葡萄球菌裂解性噬菌体PhMASA-WJ的生物学特性及其对小鼠败血症的疗效

目的探讨耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)噬菌体PhMASA-WJ的生物学特性及其对小鼠败血症的疗效,为开发高效低毒的抗菌药物奠定实验基础。方法以MRSA(USA300)为宿主菌,从地下污水中分离筛选出1株噬菌体PhMASA-WJ,双层琼脂平板培养法测定噬菌体效价,点滴法测定噬菌体裂解谱,确定最佳感染复数(multiplicity of infection,MOI),提取噬菌体全基因组并进行酶切鉴定,电镜观察噬菌体形态,并检测噬菌体的体外裂解效果、安全性及其对小鼠败血症的疗效。结果 PhMASA-WJ在USA300菌苔上形成直径1～2 mm的圆形、透明且边界清晰的噬菌斑,PhMASA-WJ效价为7. 1×10⁸ PFU/m L,其为专一裂解金黄色葡萄球菌的噬菌体,最佳MOI为0. 001,属于RNA病毒,基因组大小约23 000 bp。电镜观察显示,PhMASA-WJ属于有尾噬菌体目肌尾噬菌体科。动物实验证实,PhMASA-WJ对小鼠无毒副作用,败血症小鼠经PhMASA-WJ...     

慢病毒介导的人乳头瘤病毒16型E6基因shRNA对荷瘤裸鼠宫颈癌生长的抑制作用

目的探讨慢病毒介导的人乳头瘤病毒(Human papilloma virus,HPV)16型E6基因shRNA在体内对荷瘤裸鼠宫颈癌生长的抑制作用。方法将BALB/c-nu裸鼠随机分为空白对照组、干扰组和无义干扰组,经皮下接种宫颈癌Caski细胞(2×106个),移植瘤直径达0.5 cm时,空白对照组于瘤体局部注射PBS,干扰组和无义干扰组分别于瘤体局部注射靶向HPV16型E6基因的shRNA-PLL3.7干扰慢病毒和无义干扰慢病毒(3×108TU/ml),2周后检测瘤体大小和重量的变化,采用免疫组化法检测肿瘤组织中HPV16型E6、P53和P21蛋白的表达。结果与无义干扰组和空白对照组比较,干扰组裸鼠体内肿瘤明显缩小,瘤体重量明显降低(P<0.000 1),无义干扰组与空白对照组比较,差异无统计学意义(P>0.05);干扰组肿瘤组织中HPV16型E6蛋白的表达被抑制,P53和P21蛋白的表达水平明显升高。结论慢病毒介导的HPV16型E6基因shRNA能有效抑制动物体内宫颈癌的生长,具有潜在的开发应用前景。     

The effect of silane-based hydrophobic admixture on corrosion of reinforcing steel in concrete

The influence of a hydrophobic admixture based on silane on the corrosion resistance of steel reinforcement in concrete was studied. Sound or deliberately pre-cracked concrete specimens were manufactured with w/c of 0.45 and 0.80, both in the presence and in the absence of silane. The specimens were fully immersed in a 3.5% NaCl aqueous solution.The results, in terms of electrochemical measurements, visual observations, and weight loss measurements of steel reinforcement, show that silane blocked corrosion process in uncracked concrete specimens. On the other hand, in cracked concrete specimens, corrosion of steel reinforcements was unexpectedly more severe in hydrophobic specimens rather than in the corresponding not hydrophobic ones. It is believed that oxygen, which is needed to feed the corrosion process, diffuses faster in a gaseous phase through the open concrete porosity in the hydrophobic concrete, whereas in concrete without silane, oxygen diffuses much more slowly through the water filling the pores of the saturated concrete.     

Experimental investigations of the effect of coal type and coal burner with different oxygen supply angles on gasification characteristics

The IGCC (Integrated gasification combined cycle) is known as one of the coal fueled power generating technologies with the highest efficiency and lowest emissions. To achieve the required higher efficiency and lower emission performance, Korea's 300 MW IGCC RDD&D (Research Development, Demonstration and Dissemination) project was launched in December 2006 under the leadership of the Korea Electric Power Corporation (KEPCO), with the support of the Korea Ministry of Knowledge Economy. Our research group, the KEPCO Research Institute, set up a coal gasifier for the pilot test and conducted many experiments for the parametric study in this project.Our group focused its research on the effect of different types of coal and oxygen supply angles on the optimum O₂/coal ratio for gasification. Through this study we found that using higher oxygen content coal reduced the optimum O₂/coal ratio for gasification. However, there was no apparent relationship between the oxygen supply angle and the optimum O₂/coal ratio. And, two types of coal burners having different oxygen supply angles were used to conduct the feasibility study on a variable angle burner for the coal gasifier.     

抗旋毛虫硒蛋白T单克隆抗体的制备及其对裸鼠肝癌H7402细胞的抑制效果

目的制备抗旋毛虫与肝癌H7402细胞相关抗原硒蛋白T单克隆抗体,并观察其对裸鼠肝癌H7402细胞的抑制效果。方法用纯化的旋毛虫硒蛋白T重组蛋白常规免疫BALB/c小鼠,采用杂交瘤细胞技术制备其单克隆抗体;将肝癌H7402细胞经大腿内侧皮下接种BALB/c裸鼠,1×10^7个细胞/只,采用制备的高效价单抗,按20、50及100μg/只剂量接种裸鼠,隔日1次,共5次,评价单抗对裸鼠肝癌H7402细胞的抑制效果。结果成功获得了3株稳定分泌抗旋毛虫硒蛋白T的单抗的细胞株:4D4、4H2及4F1,效价均达1∶204800以上,其中4H2抗体效价最高;20、50及100μg/只剂量组的抑瘤率分别为51.6%、56.2%和80.3%。结论制备的抗旋毛虫与肝癌H7402细胞相关抗原硒蛋白T单克隆抗体对裸鼠体内肝癌H7402细胞具有明显的抑制效果。     

乌司他丁和泰索帝对人乳腺癌细胞MDA-MB-231中uPA、uPAR、ERK表达的影响

目的探讨乌司他丁(Ulinastatin,UTI)和泰索帝(Taxotere,TXT)对体外培养的人乳腺癌细胞MDA-MB-231中u-PA、uPAR、ERK表达的影响。方法将MDA-MB-231(ER-)细胞分为4组:UTI组(UTI 800 U/ml)、TXT组(TXT 3.7μg/ml)、UTI+TXT组(UTI 800 U/ml+TXT 3.7μg/ml)、对照组(等量生理盐水)。给药后24 h,分别采用荧光定量RT-PCR检测各组细胞中uPA、uPAR、ERK基因mRNA的水平,Western blot法检测各组细胞中uPA、uPAR、p-ERK1/2蛋白的表达水平。结果 UTI组和UTI+TXT组MDA-MB-231(ER-)细胞中uPA和uPAR基因mRNA的水平均明显低于对照组(P<0.05),而TXT组中二者的表达水平均明显高于对照组(P<0.01),各组间ERK基因mRNA的水平差异无统计学意义(P>0.05);UTI组和UTI+TXT组中uPA、uPAR和p-ERK1/2蛋白的表达水平均明显低于对照组(P<0.01),而TXT组中3种蛋白的表达水平均明显高于对照组(P<0.05)。结论UTI可抑制MDA-MB-231细胞中uPA、uPAR、p-ERK的表达,而TXT可上调三者的表达。