Ohmic heating assisted vacuum evaporation of pomegranate juice: Electrical conductivity changes

A novel electrical heating method, named as ohmic heating, was successfully integrated to vacuum evaporation system, and pomegranate juice was concentrated until its total soluble dry matter content reached to 40% by applying three different voltage gradients (7.5, 10, and 12.5 V/cm) at 180 mm Hg absolute pressure in this system. Total evaporation times were determined as 152, 78, and 53 min at the voltage gradients of 7.5, 10, and 12.5 V/cm, respectively. The concentration time of pomegranate juice was shortened about 56% by ohmic heating relative to conventional evaporation. The electrical conductivity values were increased up to reach 25% TSDM for 7.5 V/cm and at 35% TSDM for 10 V/cm, then showed a decreasing pattern. However, it was constant (0.55 ± 0.01 S/m) during evaporation process at 12.5 V/cm. It is recommended that the ohmic heating method could be successfully integrated to vacuum evaporation process to shorten the processing time significantly.Industrial relevanceOhmic heating has been utilized as alternative method for the purpose of heating, pasteurization, cooking etc., and relatively better products can be obtained by ohmic heating. Nowadays, energy efficient systems are needed in concentrated juice production. The integration of ohmic heating to the conventional vacuum systems could serve the production high quality juice concentrates with efficient use of energy. In present study, ohmic heating assisted vacuum evaporation of pomegranate juice was conducted, successfully. This method decreased the total process time for juice concentration, which is critically important for industrial scale productions. This novel method can be implemented to the fruit juice production lines by taking into account of design characteristics of ohmic systems such as electrical conductivity changes depending on both temperature and total soluble solids concentration. The effects of main process parameter, named as voltage gradient, on electrical conductivity changes during electrical heating assisted evaporation process has been also reported in the present study.     

Pulsed electric field processing effects on flavor compounds and microorganisms of orange juice

The headspace flavor compounds of fresh squeezed orange juice processed by pulsed electric field (PEF) at 30 kV/cm for 240 or 480 μs, or heat at 90°C for 1 min were isolated by a solid phase microextraction (SPME) coating and separated by gas chromatography. The average losses of flavor compounds in orange juice processed by 240, 480 μs PEF and heat process were 3.0%, 9.0% and 22.0%, respectively (P < 0.05). The flavor loss was mainly due to vacuum degassing in the PEF process. The total plate counts of control, 240, 480 μs PEF, and heat processed orange juice were 5400, 21, 19, and 4, respectively. The yeast and mold counts of control, PEF for 240, 480 μs and heat processed orange juice were 2800, 15, 9, and 4, respectively. ©     

Effect of thermosonication on bioactive compounds in watermelon juice

Freshly squeezed watermelon juice was subjected to thermosonication treatments with processing variables of temperature (25-45 °C), amplitude level (24.1-60 ??m) and processing time (2-10 min) at a constant frequency of 20 kHz and pulse durations of 5 s on and 5 s off. Hunter color values (L*, a* and b*), lycopene (LC), phenolic content (TP) and ascorbic acid (AA) content were measured. Higher retention of AA and LC was observed at low treatment conditions. AA, LC & TP decreased significantly at higher amplitude levels and at the maximum processing time. A second order response function covering the range of experimental conditions investigated was obtained for each of the compounds measured. Model predictions for color parameters and bioactive compounds were closely correlated to the experimental results obtained. Prediction models were found to be significant (p < 0.05) with low standard errors and high coefficients of determination (R²). It was concluded that thermosonication significantly influences key watermelon juice quality parameters     

Inactivation of pectin Methylesterase and Lactobacillus Plantarum by ohmic heating in pomelo juice

The use of ohmic heating (OH) to inactivate damaging elements in pomelo juice was evaluated. The work investigated the effect of frequencies (50–20 000 Hz) on pectin methylesterase (PME) and Lactobacillus plantarum (L. plantarum) in pomelo juice at 30 V cm⁻¹ of electric field strength. The results show that frequency does not affect PME inactivation (P > 0.05) but affects bacterial reduction and has the most efficiency at 60 Hz, followed by 50 Hz, and in the frequency range at or higher than 500 Hz (P < 0.05). Additionally, the inactivation kinetics of PME and L. plantarum and the impact of nonthermal factors were given. The nonthermal element has contributed to the inactivation enhancement of L. plantarum and PME to reduce the necessary treatment temperature and time. The major spoilage bacteria and enzyme destruction in pomelo juice occurred faster under OH than under conventional heating (CH) suggesting that OH can be effectively used to pasteurise pomelo juice.     

Ohmic heating treatment for Gac aril oil extraction: Effects on extraction efficiency,physical properties and some bioactive compounds

The effects of ohmic heating on Gac aril oil extraction in comparison with conventional heating were studied. The experiments were conducted using three extraction stages with the selected ratio of Gac aril powder to n-hexane (solvent) and time for each stage of 1:7 (7 h), 1:6 (6 h) and 1:5 (5 h), respectively. The aims were to (1) study the possibility of applying ohmic method in Gac aril oil extraction and (2) compare between oil extraction using ohmic and conventional treatments. It appeared that the electrical conductivities of fresh Gac aril, Gac aril powder with water and Gac aril powder with hexane ranged between 0.54 and 1.16, 0.50–1.46 and 0.18–0.31 S·m^− 1 respectively. The extraction efficiency, color characteristics and the contents of β-carotene and lycopene of Gac aril oil were enhanced by extraction with ohmic method. Three extraction stages with ohmic heating treatment at 50 °C in all stages resulted in the highest extraction efficiency (81.40%). The SEM micrographs proved that the cell wall of the Gac aril powder after extraction with ohmic heating was more ruptured than that of conventional heating. Physical properties of Gac aril powder and residues collected from different extraction methods were diverse due to their dissimilar compositions and structures.     

Influence of high-intensity pulsed electric field processing parameters on antioxidant compounds of broccoli juice

The effect of high-intensity pulsed electric field (HIPEF) processing parameters (electric field strength, treatment time, and polarity) on broccoli juice carotenoids, vitamin C, total phenolic (TP) content and antioxidant capacity (AC) was evaluated. Results obtained from HIPEF-processed broccoli juice were compared with those of thermally treated (90 °C/60 s) and untreated juices. HIPEF processing parameters influenced the relative content (RC) of bioactive compounds, and the relative AC (RAC). Maximum RC of lutein (121.2%), β-carotene (130.5%), TP (96.1%), vitamin C (90.1%) and RAC (5.9%) was reached between 25 and 35 kV/cm and from 2000 μs to 500 μs. The highest RAC and RC of bioactive compounds were observed in HIPEF treatments applied in bipolar mode, except for vitamin C. HIPEF-treated broccoli juice exhibited greater RC of bioactive compounds and RAC than juice treated by heat. HIPEF technology could be considered a promising option for preserving the antioxidant quality of broccoli juice.Industrial relevanceVegetable juices are becoming more and more popular because of their wide range of health-related compounds. Particularly, broccoli juice is attracting the food industry attention because it contains high amounts of vitamins, carotenoids and phenolic compounds, among other bioactive compounds. Broccoli juice requires treatment conditions that protect its microbial, nutritional and sensorial quality. HIPEF is a non-thermal technology for liquid food preservation that inactivates microorganisms and enzymes without compromising the nutritional and sensorial features of foods. Consequently, this technology could be used in the food industry as an alternative for thermal treatment to preserve the bioactive compounds present in vegetable juices, offering to consumers a healthy product.     

Recovery of bioactive compounds in kiwifruit juice by ultrafiltration

Food quality is not only a function of nutritional values but also of the presence of bioactive compounds exerting a positive effects on human health.This research was undertaken to study the influence of the ultrafiltration (UF) on the composition of some bioactive compounds of the kiwifruit juice in order to develop a natural product which can be used to fortify foods and beverages. At the same time the effect of transmembrane pressure (TMP) and temperature on the permeate flux was also investigated in order to identify the optimal operating conditions for the processing of the juice. An optimal TMP value occurred at 0.6–0.65 bar in different conditions of cross flow velocities. Steady-state permeate fluxes increased linearly with temperature in the range 20–30 °C.The kiwifruit juice was clarified in optimal operating conditions, according to the batch concentration mode, up to a final volume reduction factor (VRF) of 2.76.The analyses of flux decay according to fouling models reported in the literature revealed that the formation of a cake layer covering the entire surface of the membrane is the main cause of the membrane fouling.Most bioactive compounds of the depectinised kiwifruit juice were recovered in the clarified fraction of the UF process. The rejection of the UF membrane towards total phenolics was 13.5%. The recovery of glutamic, folic, ascorbic and citric acids, in the clarified juice, with respect to the initial feed, was dependent on the final VRF of the UF process: an increase of the VRF determines an increase of these compounds in the clarified juice. The rejections of the UF membrane towards these compounds were in the range 0–4.3%.

Industrial relevance

Among different substances contained in the kiwifruit a primary role, in the safeguard of the human health, is carried out by some bioactive compounds such as ascorbic, folic, citric, glutamic acids and polyphenols.This research was undertaken to study the influence of ultrafiltration on the composition of these compounds in order to develop a natural product which can be used to fortify foods and beverages.     

琯溪蜜柚果汁树脂吸附脱苦前后酚类物质含量的测定

利用高效液相色谱法测定了琯溪蜜柚果汁酚类成分的含量,并研究了吸附脱苦对琯溪蜜柚果汁中酚类物质含量的影响。结果表明,琯溪蜜柚果汁中含有绿原酸、咖啡酸、香豆酸、阿魏酸、芸香苷和柚皮苷等六种酚类物质;用高效液相色谱法分析琯溪蜜柚果汁中酚类物质,它们的加标回收率均在85.0%¹03.6%之间,相对标准偏差（RSD）均在0.48%².9%之间。经XAD-16树脂吸附脱苦后,柚汁中的香豆酸、阿魏酸、芸香苷等三种酚类成分已检测不到,而绿原酸、柚皮苷和咖啡酸的损失率分别为97.2%、93.5%、87.9%,说明树脂吸附脱苦导致琯溪蜜柚汁中绿原酸、咖啡酸、香豆酸、阿魏酸、芸香苷和柚皮苷等六种酚类物质含量严重降低。      

Industrial orange juice debittering: Impact on bioactive compounds and nutritional value

The impact of an industrial debittering process (DP) on nutritional and bioactive compounds in orange juice (OJ) was studied. The DP was aimed at removing bitter components in OJ by physical adsorption in a resin. The levels of bioactive compounds (carotenoids, ascorbic acid and phenolics), total antioxidant activity and the colour in the fresh orange juices (non-debittered) and in the debittered counterparts were measured. The results demonstrated that the carotenoid contents were not significantly affected by the treatment. However, the debittered orange juices showed a reduction (p < 0.001) of 26% in ascorbic acid, 32% in hydroxycinnamic acids, 28% of flavones and 41% of flavanones in comparison with the non-treated juices. The antioxidant activity of the hydrophilic fraction (HF) was significantly higher (p < 0.05) in untreated juice than in debittered juices. Some colour parameters (L^∗, a^∗ and h_ab) were also affected. Discriminant analysis revealed that the canonical function related to the levels of HF compounds allowed a 100% correct classifications of the different types of juices.     

高压脉冲电场杀菌技术在橙汁中的应用

食品冷杀菌技术是一类不用热能杀死微生物,不影响食品营养、质构、色泽和风味的新兴杀菌技术。主要阐述了高压脉冲电场杀菌（PEF）的基本原理和优点,讨论了其在橙汁中的应用,并对比了PEF处理和热处理（90℃,15s）对橙汁微生物的杀灭效果及橙汁理化指标的变化。     

Physicochemical parameters,bioactive compounds and microbial quality of sonicated pear juice

This study was carried out to investigate the effect of sonication on physicochemical parameters and microorganisms of pear juice. Ultrasound processing of fresh pear juice was done at fixed amplitude [70% (500 W) and frequency (25 kHz) for 0, 15, 30, 45 and 60 min at 25 °C. Total soluble solids, pH, titratable acidity and Ca and Mn remained stable, while the cloud value, ascorbic acid, total phenols, total flavonoids, total antioxidant capacity, sugar contents and Na, K, Fe and Mg showed a significant increase. Decreases in microbial population and P and Cu were also observed. It may be inferred that ultrasound processing for 60 min exhibited optimum results in terms of physicochemical and microbial quality.     

Influence of packaging material on pomegranate juice colour and bioactive compounds,during storage

Pomegranate juices were assessed after pasteurisation and storage in different packaging materials: transparent and green glass, and paperboard carton with polyethylene layers (Minibrik‐200). The main objective was to establish the influence of the container on the stability of colour and bioactive compounds (anthocyanins, ellagic acid and other non‐coloured phenols). Results showed that non‐coloured phenols and ellagic acid were quite stable during the storage period. In contrast, anthocyanins degraded, to an extent directly proportional to colour loss, less for those juices stored in glass bottles than for those stored in Minibrik. These results indicated that these paperboard carton containers are oxygen permeable, and that oxygen has a greater influence on anthocyanin, and consequently on colour degradation, than light on pomegranate juice during storage. Nevertheless, the antioxidant activity was not influenced by the packaging material employed. Copyright © 2004 Society of Chemical Industry     

Membrane damage and viability loss of Escherichia coli K-12 in apple juice treated with radio frequency electric field

The need for a nonthermal intervention technology that can achieve microbial safety without altering nutritional quality of liquid foods led to the development of a radio frequency electric fields (RFEF) process. In order to understand the mechanism of inactivation of bacteria by RFEF, apple juice purchased from a wholesale distributor was inoculated with Escherichia coli K-12 at 7.8 log CFU/ml and then treated with RFEF. The inoculated apple juice was passed through an RFEF chamber operated at 20 kHz, 15 kV/cm for 170 micros at a flow rate of 540 ml/min. Treatment condition was periodically adjusted to achieve outlet temperatures of 40, 45, 50, 55, and 60 degrees C. Samples at each outlet temperature were plated (0.1 ml) and the number of CFU per milliliter determined on nonselective and selective agar media was used to calculate the viability loss. Bacterial inactivation and viability loss occurred at all temperatures tested with 55 degrees C treatment, leading to 4-log reductions. No significant effect was observed on bacterial population in control samples treated at 55 degrees C with a low-RFEF (0.15 kV/cm) field strength. These observations suggest that the 4-log reduction in samples treated at 15 kV/cm was entirely due to nonthermal effect. RFEF treatment resulted in membrane damage of the bacteria, leading to the efflux of intracellular ATP and UV-absorbing materials. Populations of injured bacteria recovered immediately (<30 min) from the treated apple juice averaged 0.43 log and were below detection after 1 h of RFEF treatment and determination using selective plates (tryptic soy agar containing 5% sodium chloride). The results of this study suggest that mechanism of inactivation of RFEF is by disruption of the bacterial surface structure leading to the damage and leakage of intracellular biological active compounds.     

Effects of pulsed thermosonication treatment on fungal growth and bioactive compounds of Berberis vulgaris juice

In this study, the single and combined effects of pulsed‐ultrasound amplitude (100 W, 30 kHz with 25 and 50% amplitude levels for up to 30 min) and temperature (65 °C and 75 °C) on fungal growth, total phenolics, flavonoids and antioxidant activity of Berberis vulgaris juice were investigated. The combination of pulsed ultrasound at 50% amplitude for 30 min at temperature of 75 °C as the most effective treatment was resulted in a reduction of about?3.083 ± 0.02, ?3.04 ± 0.03, ?3.10 ± 0.01 and ?2.88 ± 0.02 log (N/N₀) on S. cerevisiae, A. flavus, A. versicolor and B. fulva, respectively. Additionally, the highest total phenolics, flavonoids and antioxidant activity were noted for the barberry juice after 30 min of sonication with 50% pulsed‐ultrasound amplitude at either 65 °C or 75 °C. Pulsed thermosonication is a promising technology to extend the shelf life of B. vulgaris fruit juice because of the improvement of antioxidant activity as well as microbial quality.     

Effect of thermosonication on peroxidase,pectin methylesterase activities and on bioactive compounds in custard apple juice

Custard apple juice faces long term storage challenges such as pink discoloration and bitterness due to peroxidase activity. Therefore, inactivation of peroxidase becomes a necessity for longer shelf life of custard apple and its application in food preparations. Heat processing has certain pitfalls such as loss in nutritional contents of custard apple juice, thereby adversely affecting the sensory quality. In the present study, thermosonication of custard apple juice at a constant temperature of 30?±?1?°C (20 kHz, 67.84 W/cm²) from 0 to 40 min has been studied with respect to peroxidase and pectin methylesterase inactivation. Effect of thermosonication has also been studied on bioactive components such as total phenolics and flavonoids along with enzyme inactivation. Complete inactivation of peroxidase and pectin methylesterase with 0% residual activity was achieved within 40 min and 50 s, respectively. Total phenolics were found to increase from 70.9?±?1.6 to 81.7?±?2.5 mg GAE/g. Sonication was found to have no significant effect (P?>?0.05) on pH, °Brix, titratable acidity. Ascorbic acid content remained relatively unaffected during sonication. Hence, thermosonication may be employed as food processing technique where retention of bioactive components have been achieved along with safety and quality attributes.