Effect of innovative pre-treatments on the mitigation of acrylamide formation in potato chips

The aim of this research was to evaluate the reduction of acrylamide (AA) formation in potato chips applying innovative pre-treatments. Raw potato slices were subjected for 5 and 15 min to: dipping in water; dipping in Aureobasidium pullulans L1 yeast water suspension; dipping in water or in yeast water suspension after pulsed electric fields (PEF) in order to investigate a possible synergistic effect of pre-treatments. The raw potato samples were analysed for AA precursors and, after frying, for AA by using HPLC-MS/MS. In addition, the final potato chips main quality parameters were evaluated.Compared to untreated potato chips, yeast treatment promoted a reduction of AA formation mainly at the longest dipping time (15 min). PEF treatment followed by water dipping was the most effective in reducing AA for both the studied treatment times. The combination of PEF and yeast treatments led only a slight reduction of AA formation.Industrial relevanceThe Commission Regulation (EU) 2017/2158 has established new acrylamide (AA) benchmark levels in different foods due to its negative classification as “probably carcinogenic to human”. For this reason, food industries are interested in developing different processing methods to reduce the AA formation and at the same time to maintain an acceptable quality of final products. Fried potatoes (French fries and chips) are the most vulnerable foods in terms of high content of AA formation, being rich in the main Maillard reaction substrates, such as asparagine and reducing sugars, and characterized by a high surface to volume ratio. Among the strategies recently suggested for the reduction of AA in potato chips, pulsed electric fields (PEF) and yeast pre-treatments are very promising, having the potentiality to reduce AA precursors in raw potato tissues. In this study the possibility to apply yeast and PEF pre-treatments on raw potato slices with suitable processing times for a possible industrial application were evaluated.     

低温真空油炸薯片生产工艺及其贮藏条件的研究

对马铃薯片进行低温真空油炸生产工艺的基础性研究,分析薯片在贮藏过程中水分、脂肪和VC含量的变化,并探讨等温吸湿规律。通过单因素试验确定最优的油炸工艺参数为温度105℃,时间20min,真空度0.090MPa;离心脱油的最佳条件为脱油转速400～500r/min,时间5～7min,真空度0.090MPa。研究结果对低温真空油炸薯片技术具有很好的指导和促进作用,同时改善了现在油炸薯片的品质和提高了能源利用率。     

Effect of pulsed electric fields on the structure and frying quality of “kumara” sweet potato tubers

The aim of this research was to study the effect of pulsed electric fields (PEF) on the microstructure of “kumara” sweet potato (Ipomoea batatas cv. Owairaka) and its quality after frying. Whole sweet potato tubers were treated at different electric field strengths ranging from 0.3 to 1.2 kV/cm with specific energy levels between 0.5 and 22 kJ/kg. Cell viability was determined using tetrazolium staining to investigate the uniformity of the PEF effect across tubers. Based on the patterns of viable cells it was observed that the effect of PEF was not homogeneous across the tuber. This result was also supported by the pattern of enzymatic browning due to PEF facilitating the reaction of polyphenoloxidase and phenols. PEF treatment resulted in significant softening of the ground tissues, but not on the dermal tissues, as determined by texture analysis. With respect to frying quality, tubers pre-treated with PEF at electric field strength of 1.2 kV/cm and fried at 190 °C had an 18% lower oil content than non-PEF treated samples. The kinetics of browning as a function of frying time could be described by a fractional conversion model. The activation energy (Ea) of the browning rate during frying increased (more temperature sensitive) due to PEF pretreatment at 0.5 kV/cm and 1.2 kV/cm. It implies that PEF pretreatment allows frying the potato chips at lower temperature in order to achieve the same brown colour intensity as the non-PEF treated tubers. This study shows clearly that PEF could reduce the energy required for cutting and frying of kumara.Industrial relevanceThis study provides evidence that the effect of PEF processing on whole kumara tubers is not uniform, demonstrating heterogenous distribution. These findings provide important information for food industry to design appropriate PEF processing conditions for solid materials. More importantly, PEF treatment reduced the energy required for cutting and frying of kumara, and reduced the oil content in the fried kumara chips.     

Pulsed electric fields and their impact on the diffusion characteristics of potato slices

Mass transfer in potato slices and strips after Pulsed Electric Fields (PEF) treatment was examined to evaluate potential application of PEF in potato processing. PEF treatment on cell material leads to pore formation in cell membrane and thus modifies diffusion of intra- and extracellular media. Results showed enhanced release of intracellular molecules from permeabilized tissue as well as improved uptake of low molecular substances into the sample. Sugar, one substrate for the Maillard reaction, was decreased in PEF treated potatoes, while conductivity increased after electroporation and soaking in sodium chloride solution, indicating the improved diffusion of salt caused by PEF. Higher release of cell liquid during drying of PEF treated potatoes was noticed in comparison to untreated potato slices. This effect increased with the treatment intensity. Furthermore, it was revealed that PEF application leads to a distinct reduction of fat content after deep fat frying and thus provides a potential for the production of low-fat French fries. It can be presumed that PEF is a capable assistance to thermal treatments in the processing of potato chips or French fries for the achievement of structural modifications and improved process conditions.     

茶多酚在油炸过程中对马铃薯片品质及其贮藏稳定性的影响

以感官品质、质构特性、理化特性、风味特性以及丙烯酰胺含量为指标,研究茶多酚在连续油炸过程中对马铃薯片品质的影响,并进一步探究茶多酚对马铃薯片贮藏稳定性的影响。结果表明,茶多酚对连续油炸过程中马铃薯片感官品质及硬度值的下降具有抑制作用;茶多酚对连续油炸过程中马铃薯片水分含量和含油率的增加具有明显的抑制作用,并且降低了薯片的含油率,茶多酚组T₂₄批次的马铃薯片含油率为41.96%±2.00%,显著低于空白组的46.29%±1.06%(P<0.05)。同时,与空白对照组相比,茶多酚抑制了马铃薯片劣变风味的产生,明显抑制了马铃薯片中有害物丙烯酰胺的生成。此外,茶多酚能够增加油炸马铃薯片的贮藏稳定性,平均货架期能够延长8 d。初步分析,茶多酚在油炸过程中对油脂品质的保护作用是其能够提高马铃薯片品质的重要原因。     

栀子果油煎炸过程中的品质变化及对油条和薯条的感官影响

为了探究栀子果油煎炸过程中的品质变化,本文选择薯条和油条两种代表性煎炸食材,将栀子果油在煎炸20h内每隔2h的酸价、羰基价、过氧化值和极性组分与大豆油、棕榈油在同等条件下煎炸的理化指标进行比较,结果表明：棕榈油煎炸后的品质较高,栀子果油和大豆油比较相似,栀子果油可以作为煎炸油。但栀子果油连续煎炸12 h时极性组分超过国家现有煎炸油的卫生标准,因此其连续煎炸时间不能超过12 h。同时对1、10、20h煎炸出来的油条和薯条进行感官评价,结果表明：煎炸食材相同时,三种食用油的感官评分无显著性差异（P>0.05）,说明栀子果油对油条和薯条的品质无不良影响,适合作为煎炸油。     

Ultrasonic-assisted leaching of glucose and fructose as an alternative mitigation technology of acrylamide and 5- hydroxymethylfurfural in potato chips

The effect of ultrasound (480 W, 40 kHz) on the leaching of reducing sugars during the water soaking of potatoes slices (60, 70 and 80 °C- 1, 8 and 15 min) was investigated to reduce the formation of acrylamide (AA) and 5-hydroxymethylfurfural (5-HMF) in potato chips.Ultrasound (US) influenced abruptly the reducing sugar leaching during the first 15 min, significantly increasing their extraction rate (glucose: 60%, fructose: 30%) at all evaluated temperatures. When potato slices were treated with US, the formation of AA (~95%) and 5-HMF (~96%) were reduced significantly after frying. Although AA content did not correlate with glucose and fructose concentrations, 5-HMF did (r²: 0.80 and 0.83, respectively), probably because reducing sugars are their main precursors. The AA and 5-HMF concentrations of potato chips presented good correlation coefficient (r²:0.76), suggesting the use of 5-hydroxymethylfurfural as an acrylamide indicator for potato chips.     

Changes in oil and potato chips during frying

The effect of frying of potato chips (batch processing during 12 h) on the chemical characteristics and fatty acid composition of the used oil were determined. Samples of the fried oil and potato chips were taken at various time periods. The volatile carbonyl compounds of the aroma concentrate of each sample were isolated and their oxime derevatives were prepared and subsequently estimated by UV absorption for the total oximes and conjugated diene carbonyl oximes. The development of the volatile carbonyl compounds in both frying oil and fried potato chips was increased on continued frying. Concerning the frying oil this increase was found to be in coincident with the decrease in the linoleic and linolenic acid percent.     

Color changes and acrylamide formation in fried potato slices

The objective of this work was to study the kinetics of browning during deep-fat frying of blanched and unblanched potato chips by using the dynamic method and to find a relationship between browning development and acrylamide formation. Prior to frying, potato slices were blanched in hot water at 85 °C for 3.5 min. Unblanched slices were used as the control. Control and blanched potato slices (Panda variety, diameter: 37 mm, width: 2.2 mm) were fried at 120, 150 and 180 °C until reaching moisture contents of ∼1.8% (total basis) and their acrylamide content and final color were measured. Color changes were recorded at different sampling times during frying at the three mentioned temperatures using the chromatic redness parameter a¹. Experimental data of surface temperature, moisture content and color change in potato chips during frying were fit to empirical relationships, with correlation coefficients greater than 90%. A first-order rate equation was used to model the kinetics of color change. In all cases, the Arrhenius activation energy decreases alongside with decreasing chip moisture content. Blanching reduced acrylamide formation in potato chips in ∼64% (average value) in comparison with control chips at the three oil temperatures tested. For the two pre-treatments studied, average acrylamide content increased ∼58 times as the frying temperature increased from 120 to 180 °C. There was a linear correlation between acrylamide content of potato chips and their color represented by the redness component a¹ in the range of the temperatures studied.     

Quality changes of edible oils during vacuum and atmospheric frying of potato chips

Demand for safe and nutritionally rich fried products is gaining a momentum among consumers, leading to the increased consumption of vacuum fried products. The impact of vacuum frying (VF)(110 °C, 40 kPa) on chemical composition of food, fatty acid profile, microstructure, oxidative stability and sensory attributes was assessed and differentiated with that of atmospheric frying(AF) (180 °C). The potato slices were fried in mustard and soyabean oil used repetitively for 25 h. The oil content of VF potato chips was lower (15.18%) than AF chips (18.98%), however water loss in AF chips was higher than VF chips by 1.63-fold. VF significantly prevented the PUFA degradation, minimizes transfatty acid (TFA) formation and maintain a low C18:2/C16:0 ratio as compared to AF. VF Chips fried in soyabean oil show an increase in TFA content from 2.15 to 2.63% and a decrease in PUFA from 51.57 to 45.16% as compared to AF chips where TFA content increased from 2.15 to 3.72% and PUFA shows a higher reduction from 51.57 to 37.69% at the end of 25 h of frying. This indicate that in AF, oil is safe for use upto 10 cycles of frying, while as in VF, the same oil can be used for upto 40 cycles of frying without quality deteoriation. Sensorial analysis revealed that VF chips retain a better colour, taste and flavour but were less crispy than that of AF chips. These findings validate the application of vacuum frying technology for the production of high-quality foods with lesser degradation of frying oil.Industrial relevanceFood manufacturers are now impelled by the health-conscious consumer base for the production of healthy food products. The toxic effect of foods fried in degraded oils on human health is now widely known and thus the production of safe fried foods is the need of hour globally. In this context, vacuum frying is the most feasible approach for the production of quality fried products retaining the natural colour, flavour, sensory and nutritional properties better than that of atmospheric frying. Vacuum frying causes the least degradation of fatty acid of the frying oil and the fried potatoes, producing healthy potato chips. Therefore, the oil used for vacuum frying have a greater shelf life and oxidative stability than atmospheric frying. However, the higher installation cost of vacuum fryer still limits its use in the street fried food market, where degradation of oil is more likely. Thus, for its widespread commercialisation in developing countries, steps should be taken both by government and manufacturing companies to reduce the installation costs.     

复合马铃薯片油炸工艺研究

以马铃薯淀粉和马铃薯全粉为主要原料,对复合马铃薯片的油炸工艺参数进行了研究。采用正交试验,确定了生产低含油量、低丙烯酰胺(AA)含量、高脆度复合薯片的最佳油炸工艺参数,即油炸前坯料的含水量控制在20%左右,含盐量2%左右,油温以160℃为佳,炸制时间为40 s,油炸薯片丙烯酰胺含量为0.28 mg/kg,在市售薯片基础上降低26.32%;含油量为23.36%,降低了1.47个百分点。     

Effect of Cooking Method (Baking Compared with Frying) on Acrylamide Level of Potato Chips

ABSTRACT:  The effect of cooking method (baking compared with frying) on acrylamide level of potato chips was investigated in this study. Baking and frying experiments were conducted at 170, 180, and 190 °C using potato slices with a thickness of 1.4 mm. Raw potatoes were analyzed for reducing sugars and asparagine. Surface and internal temperatures of potato slices were monitored during the experiments to better explain the results. Fried and baked chips were analyzed for acrylamide content using an LC-MS method. The results showed that acrylamide level of potato chips prepared by frying increased with frying temperature (19.6 ng/g at 170 °C, 39 ng/g at 180 °C, and 95 ng/g at 190 °C). In baking, however, the highest acrylamide level was observed in potato chips prepared at 170 °C (47.8 ng/g at 170 °C, 19.3 ng/g at 180 °C, and 29.7 ng/g at 190 °C). The results showed that baking at 170 °C more than doubled the acrylamide amount that formed upon frying at the same temperature, whereas at 180 and 190 °C, the acrylamide levels of chips prepared by baking were lower than their fried counterparts.     

稻米油调和油煎炸应用研究

通过与棕榈油对比180℃下炸制薯片以及评估薯片感官喜好度、质构、口感及粘牙性等差异来评价稻米油调和油的煎炸应用性能。结果表明:采用稻米油调和油煎炸的薯片中多不饱和脂肪酸含量显著高于棕榈油(P<0.05),且其薯片的整体感官喜好度、质构口感、酥脆度、粘牙喜好度,均显著优于棕榈油薯片(P<0.05)。在加速氧化条件下,采用稻米油调和油炸制的薯片的酸价、过氧化值均与棕榈油炸制的薯片无显著性差异(P>0.05),且远低于GB 16565-2003的规定。在加速存储过程中,谷维素却得到了很好的保留。总之,稻米油调和油具有良好的煎炸性能。     

Vacuum frying as a route to produce novel snacks with desired quality attributes according to new health trends

Consumers look for products that contribute to their wellness and health, however, even health-conscious consumers are not willing to sacrifice organoleptic properties, and intense full-flavor snacks remain an important trend. The objective of this study was to examine most important quality parameters of vacuum (1.92 inHg) and atmospheric-fried carrot, potato, and apple slices to determine specific advantages of vacuum technology. Slices were fried using equivalent thermal driving forces, maintaining a constant difference between oil temperature and the boiling point of water at the working pressure (ΔT = 60 and 80 °C). This resulted in frying temperatures of 160 and 180 °C, and 98 and 118 °C, for atmospheric and vacuum frying, respectively. Vacuum-fried carrot and potato chips absorbed about 50% less oil than atmospheric-fried chips, whereas vacuum-fried apple chips reduced oil absorption by 25%. Total carotenoids and ascorbic acid (AA) were greatly preserved during vacuum frying. Carrot chips vacuum fried at 98 °C retained about 90% of total carotenoids, whereas potato and apple slices vacuum fried at 98 °C, preserved around 95% of their initial AA content. Interestingly, results showed that the antioxidant capacity of chips may be related to both the presence of natural antioxidants and brown pigments developed at elevated temperatures. PRACTICAL APPLICATION: A way to reduce detrimental effects of deep-fat frying is through operating-pressure reduction, the essence behind vacuum deep-fat frying. In this way, it is possible to remove product moisture at a low temperature in a low-oxygen environment. The objective of this research was to study the effect of oil temperature reduction when vacuum frying traditional (potatoes) and nontraditional products (carrots and apples) on most important quality attributes (vitamins, color, and oil uptake). Results are promising and show that vacuum frying can be an alternative to produce nutritious and novel snacks with desired quality attributes, since vitamins and color were greatly preserved and oil absorption could be substantially reduced.     

Reduction of Acrylamide Formation in Potato Chips by Low-temperature Vacuum Frying

ABSTRACT: Potatoes and other foods that have a high content of the amino acid asparagine and a high accumulation of reducing sugars are subject to the formation of acrylamide upon frying. The objectives of this research were (1) to analyze the level of acrylamide formed during deep-fat frying of potato chips and (2) to evaluate means of reducing acrylamide in potato chips by using different potato cultivars and vacuum frying. Several potato cultivars were used in this research, including Innovator (I), NDTX 4930–5W (N), ATX 854 04–8W (ATw), Atlantic (A), Shepody (S), ATX847806–2Ru (ATr), and White-Rose (W). An electric bench-top (atmospheric conditions)-type fryer was used to fry the potatoes. Three temperatures were used: 150 °C, 165 °C, and 180 °C. The vacuum frying experiments were performed at 118 °C, 125 °C, and 140 °C and a vacuum pressure of 10 Torr. The potatoes were sliced (1.5-mm thick) and fried for different lengths of times. For potatoes fried at 165 °C (for 4 min) at atmospheric conditions, the acrylamide contents were 5021 ± 55 ppb (W), 552 ± 25 ppb (I), 358 ± 50 ppb (N), 397 ± 25 ppb (ATw), 646 ± 55 ppb (A), 466 ± 15 ppb(S), and 537 ± 14 ppb (ATr). Vacuum frying reduced acrylamide formation by 94%. Results showed that both cultivar and modified frying systems can play an important role in reducing acrylamide formation in fried potatoes. As the frying temperature decreased from 180 °C to 165 °C, acrylamide content in potato chips reduced by 51% during traditional frying and by 63% as the temperature decreased from 140 °C to 125 °C in vacuum frying. Increased frying time increased acrylamide formation during traditional frying for all temperatures and frying methods analyzed. However, the effect on acrylamide concentration was greater for the traditional frying than the vacuum frying. Keywords: acrylamide, vacuum, frying, potato, temperature     

响应面法优化低脂甘薯脆片加工工艺

以红心甘薯为原料,优化低脂甘薯片生产过程中漂烫、热风干燥、油炸等关键工艺参数,以产品含油率和色差(L*值和b*值)为评价指标,并通过响应面优化确定最优的低脂甘薯片生产工艺.结果表明,较优漂烫工艺条件为:漂烫温度70℃,漂烫时间7 min;较优的热风干燥工艺条件为:热风干燥温度70℃,热风干燥时间40 min;最优油炸工...     

Modeling of acrylamide formation and browning ratio in potato chips by artificial neural network

The artificial neural network (ANN) modeling approach was used to predict acrylamide formation and browning ratio (%) in potato chips as influenced by time x temperature covariants. A series of feed-forward type network models with back-propagation training algorithm were developed. Among various network configurations, 4-5-3-2 configuration was found as the best performing network topology. Four neurons in the input layer were reflecting the asparagine concentration, glucose concentration, frying temperature, and frying time. The output layer had two neurons representing acrylamide concentration and browning ratio of potato chips. The ANN modeling approach was shown to successfully predict acrylamide concentration (R = 0.992) and browning ratio (R = 0.997) of potato chips during frying at different temperatures in time-dependent manner for potatoes having different concentrations of asparagine and glucose. It was concluded that ANN modeling is a useful predictive tool which considers only the input and output variables rather than the complex chemistry.     

Effect of superheated steam prefrying treatment on the quality of potato chips

Superheated steam drying (SSD) was used as a prefrying treatment prior to deep‐fat frying for potato slices. The effect of SSD at 130, 150 or 180 °C and steam velocity of 2.0 m s^?1 on the fat uptake, colour and texture of fried potato chips was evaluated; microstructure and degree of starch gelatinization were also evaluated to help explain the fat uptake results. SSD and frying yielded potato chips with the fat content from 0.263 ± 0.002 to 0.304 ± 0.002 kg kg^?1 (d.b.), while frying without SSD led to chips with the fat content as high as 0.359 ± 0.003 kg kg^?1 (d.b.). SSD did not promote starch gelatinization. Lower fat uptake was correlated to modified surface structure and lower moisture content of potato slices prior to frying. Frying with/without SSD pretreatment yielded potato chips of similar hardness, crispness and lightness. On the other hand, SSD significantly increased redness and yellowness of the fried chips.     

Deterioration of sunflower seed oil under simulated frying conditions and during small-scale frying of potato chips

The changes occurring in sunflower seed oil under simulated frying conditions and during potato chip frying were monitored. The changes monitored were in: (a) fatty acid composition, (b) triglyceride content, (c) octanoate content, (d) titratable acidity, and (e) colour. To simulate frying conditions either argon or air, with or without steam, was bubbled through the hot oil. For the frying of potato chips, two methods of frying were used, viz continuous frying throughout 1 day and intermittent frying over a total of 10 days. It was found that whenever the hot oil was exposed to air, whether during simulated frying or the frying of potato chips, there was evidence of oxidative deterioration. Steam appeared to have an antioxidant effect in the simulated frying experiments. In all experiments the molar yield of acidity was comparable in magnitude with that of the octanoate. The correlation of octanoate production with the losses of linoleate and triglyceride is discussed.     

Oil uptake and texture development in fried potato slices

The objective of this work was to study oil absorption and the kinetics of texture development of fried potato slices during frying. Prior to frying, potato slices were blanched in hot water at 85 °C for 3.5 min. Unblanched slices were used as the control. Control and blanched potato slices (Panda variety, diameter: 37 mm, width: 2.2 mm) were fried at 120, 150 and 180 °C until reaching moisture contents of 1.8% (total basis) and their texture and oil content were measured periodically. Oil uptake was higher in 15% for blanched samples than for control samples after 20 s of frying. Besides, the higher the frying temperature, the lower the oil absorption in control samples. Textural changes in fried potato slices were followed by the parameter maximum force (MF) extracted from the force vs. distance curves corresponding to different sampling times. Normalized maximum force (MF*) was used in modeling textural changes in the potato slices during frying in both the initial tissue softening process and the later crust development process. Higher temperatures accelerated these processes; however neither the temperature nor the pre-treatment had a significant effect (P > 0.05) over the final texture of the fried potato chips.