Production of edible mycoprotein using agroindustrial wastes: Influence on nutritional,chemical and biological properties

The production of Agaricus blazei, Auricularia fuscosuccinea and Pleurotus albidus mycoproteins in brewer-spent grain and grape bagasse by solid-state cultivation was studied, including an investigation of their nutritional value and biological activity. The production of P. albidus mycoprotein in brewer-spent grain resulted in the highest values of protein (22.6 g.100 g⁻¹), total amino acids (78.54 mg.g⁻¹), ergosterol (0.39 ± 0.01 mg.g⁻¹) and mycelial biomass (125.60 ± 3.30 mg.g⁻¹). The A. fuscosuccinea mycoprotein in brewer-spent grain resulted in the highest value of total phenolics (3.80 mg EAG/g) and higher antioxidant activity. Using grape bagasse, the P. albidus mycoprotein increased 23.9% proteins, reduced 15.5% and 77% total fats and phenolics, respectively. Anti-hyperglycaemic activity, assessed by α-glycosidase inhibition in vitro, was observed in the P. albidus and A. blazei mycoproteins produced in brewer-spent grain (inhibition of α-glycosidase >98%). Mycoproteins produced in brewer-spent grain and grape bagasse could be a strategy to increase the nutritional value and biological activity of these agroindustrial residues.     

Cookie making behavior of wheat–barley flour blends and effects on antioxidant properties

Refined wheat flour was replaced with whole barley flour at varying levels and the blends were evaluated for their cookie making behavior. The spread factor of cookies decreased as the proportion of barley flour increased while snap force and water activity increased significantly upto 114.7 N and 0.397 in only barley flour cookies. Increasing levels of barley flour lead to a significant decrease in L^∗ and b^∗ values of cookie dough. Peak viscosity (PV) and final viscosity (FV) increased significantly as the levels of barley flour increased. A significant increase in antioxidant activity (AOA), total phenolic content (TPC), metal chelating activity (MCA), reducing power (RP) and total flavonoid content (TFC) was observed as the proportion of barley flour increased. Baking lead to a significant decrease in TPC and TFC whereas AOA, MCA and RP increased. Baking lead to a significant increase in the non-enzymatic browning index of cookies.     

Quality and antioxidant properties of bread containing turmeric (Curcuma longa L.) cultivated in South Korea

Turmeric (Curcuma longa L.) powder was used to substitute 0%, 2%, 4%, 6% and 8% of wheat flour for making turmeric wheat breads. Proximate composition, physical quality, functional components (curcumin and total phenols) and antioxidant properties of breads containing turmeric were analysed and compared with those of wheat bread. Hardness, crumb colour a and b values, curcumin content and total phenolic contents of breads significantly increased with the addition of turmeric powder. Water activity, specific volume and crumb colour L value of breads decreased with the addition of turmeric powder. Breads containing turmeric powder also showed good antioxidant activity as tested by the β-carotene-linoleate bleaching assay. A 4% substitution of wheat flour with turmeric powder showed acceptable sensory scores which were comparable to wheat bread. Breads containing turmeric powder can thus be developed as a health-promoting functional food.     

Antioxidant capacity,phenolic compound content and sensory properties of cookies produced from organic grape peel (Vitis labrusca) flour

The by-products of the food industry can be used as nutrient source to improve the nutritional characteristics of other food products. This study aimed to investigate the chemical composition, antioxidant activity and phenolic compound content of organic grape peel flour in cookies using response surface methodology. Composition analysis using different assays (DPPH, ABTS, FRAP, ORAC) revealed that this flour was low in calories (180.22 ± 0.25 kcal%), with low dietary fibre content (50.10 ± 0.01 g%) and high antioxidant capacity. The total phenolic compound analysis showed high values of phenolic compounds (1063.58 ± 8.09 mg%), consisting of resveratrol (54.27 ± 0.79 mg%), epicatechin (43.94 ± 0.90 mg%) and chlorogenic acid (35.70 ± 1.47 mg%). A 2³ factorial experimental design was formulated to optimise the ingredients for cookie production. The resulting cookies presented high acceptance and good sensory properties, confirming that organic grape flour did not interfere with product acceptability. Thus, cookies with an acceptable quality and improved nutrition can be prepared using organic grape peel flour.     

Effect of pulse flours on the physiochemical characteristics and sensory acceptance of baked crackers

Pulse flours offer nutritional alternatives to wheat flour in the production of baked snacks due to their high protein and fibre levels and low glycaemic index. In this study, broad‐bean (Vicia faba), yellow‐pea and green‐pea (Pisum sativum) flours were each blended with wheat flour at 40% in the formulation of chemically leavened crackers. The effects of flour type and baking time on the physiochemical properties, sensory acceptability, nutritional composition and antioxidant activity of the crackers were observed in comparison with 100% wheat crackers. Broad‐bean crackers had the highest protein content and antioxidant activity (13 g per 100 g DM and 38.8 mgAAE per 100 g DM, respectively). Yellow‐pea crackers had the highest fibre content (12 g per 100 g DM). Physical dimensions and colour attributes were significantly affected by pulse‐flour substitution. Yellow‐pea and broad‐bean crackers were significantly preferred by consumers compared to the control, demonstrating the potential application of these flours to improve the eating quality and nutritional profile of crackers.     

Rice bran-soy blends as protein supplements in cookies

The effect of incorporating one of three stabilized full-fat rice brans in combination with each of four soy ingredients in a sugar-snap cookie was investigated. The four soy ingredients in a sugar-snap cookie were two soyflours (full-fat and defatted), a soy concentrate and a soy isolate. Rice bran and soy were added in place of wheat flour to increase protein content from 6% to a minimum of 9%, and the cookies were compared with each other and with a 100% wheat flour cookie. Quality characteristics evaluated included protein, spread, physical texture and colour, and sensory preference by ranking for colour, crispness and flavour. In general, the rice bran-soy cookies had less spread (even with added shortening, water and emulsifier), were less crisp and more chewy, and were somewhat darker in colour than the 100% wheat flour cookie. The soy isolate, USDA and Protex 20-S cookies were as acceptable in colour, crispness and flavour as the control.     

Chemical composition, functional properties and baking potential of African breadfruit kernel and wheat flour blends

The chemical composition and functional properties of African breadfruit kernel flour (ABKF), wheat flour (WF) and their blends were determined. Cookies prepared from the blends were evaluated for their protein contents, physical and sensory characteristics. The flour blends had higher protein, fat and ash contents than WF. The level of these nutrients improved with increased amounts of ABKF in the blends. Foaming, water and oil absorption capacities of the flour blends increased, while the foam stability decreased with increased level of ABKF. The protein contents of the composite flour cookies were higher than that of the all WF cookie. There were no significant differences (P > 0.05) in the flavour of the composite flour cookies and that of all WF cookies. However, sensory scores differed significantly (P < 0.05) amongst them for colour, texture and overall acceptability.     

Physical and nutritional evaluation of wheat cookies supplemented with pulse flours of different particle sizes

With the aim to develop functionally and nutritionally improved cookies, the influence of the total or partial replacement of wheat flour by pulses flours on the quality characteristics of cookies was analysed. Blends containing 25, 50, 75 and 100 g/100 g of navy bean, pinto bean, green lentil and commercial yellow pea flours were prepared. Green lentil, navy and pinto bean flours were used at two degrees of milling (fine and coarse). The incorporation of pulse flours significantly affected the physical and chemical parameters of the cookies. Incorporation of fine flours remarkably increased cookies’ hardness and decreased spread while coarse flours marginally reduced both parameters. The greatest impact on the physical characteristics was observed with the incorporation of green lentil flour, where cookies made with coarse flour were of unacceptable structure and were sticky to handle. Cookies baked with pulse flours were higher in protein level and showed increased antioxidant activity compared to the control. Overall analysis indicated that cookies with acceptable physical characteristics and improved nutritional profile could be produced with partial or complete replacement of the wheat flour.     

Effect of dried guduchi (Tinospora cordifolia) leaf powder on rheological,organoleptic and nutritional characteristics of cookies

Tinospora cordifolia (Guduchi) is a shrub with various medicinal properties like, antioxidant, anti-diabetic, anti-inflammatory, antihepatotoxic, etc., and widely used in Ayurvedic preparations. Use of dried Tinospora leaf powder (DTLP) at the levels of 2.5, 5.0 and 7.5% on the rheological characteristics of wheat flour; organoleptic and nutritional characteristics of cookies was studied. Incorporation of increasing amount of DTLP from 0 to 7.5% increased farinograph water absorption, decreased dough stability; increased amylograph gelatinization temperature, decreased peak viscosity; increased hardness, decreased cohesiveness and springiness of cookie dough; decreased spread ratio and increased breaking strength of the cookies. Sensory evaluation showed that cookies incorporated with 5% DTLP were acceptable. Addition of DTLP increased the protein, dietary fiber, iron, calcium, radical scavenging activity and β-carotene contents of the cookies. The results showed the possibility of utilizing DTLP to improve the nutritional properties of cookies.     

In vitro bioaccessibility of avenanthramides in cookies made with malted oat flours

This study evaluated the effects of malting and in vitro digestion on the bioaccessibility of six avenanthramides (AVNs), soluble phenols (SPs) and antioxidant capacity (ORAC) in cookies (3B, 4B, 5B) prepared with oat flour malted for three (M3), four (M4), five (M5) days, respectively. Control cookies (CTRL) were made with non-malted oat flour (M0). Five-days malting increased the AVN content of native kernels up to 10-folds, whereas SPs and ORAC tripled their values. After the in vitro digestion, AVNs showed their bioaccessibility in the following order: 5B > 4B > 3B, with a recovery of 1703 μg AVNs per 50 g portion of 5B cookies, compared to 135 μg of CTRL. Results showed that the inclusion of 27% malted oat flour was effective to formulate functional cookies with satisfactory technological properties, high AVN content and bioaccessibility.     

Olive stone powder: A potential source of fiber and antioxidant and its effect on the rheological characteristics of biscuit dough and quality

Being waste products of olive fruits, olive stones could be used as a healthy functional food ingredient thanks to their rich antioxidant properties, nutrients and dietary fibers. In this study, wheat flour was substituted by olive stone powder at levels of 0, 5, 10 and 15%. The rheological properties of biscuit dough and proximate composition, color values, total phenolic content, antioxidant activity, textural characteristics and sensorial properties of biscuit samples were investigated. Wheat flour replacement by olive stone powder increased antioxidant activity, fat and fiber content of biscuit samples (p < 0.05). The biscuits prepared with addition of 15% olive stone powder had 30.44 ± 0.03% DPPH radical scavenging activity, 11.22 ± 0.09% crude fiber, and 26.32 ± 0.22% fat. According to results of sensory analysis, wheat flour could be substituted up to 15% by olive stone powder to prepare biscuits without causing unacceptable product in terms of sensory properties.     

Nutritional assessment of cookies supplemented with defatted wheat germ

Replacement of wheat flour with defatted wheat germ (DFWG) at levels of 0–25% was investigated for its effect on functional and nutritional properties of cookies. The crude protein content of DFWG was as high as 27.8% with a highly valuable amino acid profile, rich in essential amino acids, especially lysine (2.32 g/100 g). The physicochemical and sensory evaluation of cookies, revealed that up to 15% substitution of wheat flour with DFWG produced acceptable cookies similar to the control (100% wheat flour) cookies. The protein quality of the cookies was assessed through weanling albino rats by feeding a diet of cookies for 10 days, which was formulated to supply 10% protein, with a casein diet as a control. The cookies containing 15% DFWG, were best regarding protein bioavailability in rats. The protein efficiency ratio (PER), net protein utilization (NPU), biological value (BV) and true digestibility (TD) differed significantly among diets containing cookies with 0–10% DFWG, and casein diet when fed to rats. Diets containing 15% DFWG have values, of these parameters, similar to the casein diet.     

Physical properties and sensory acceptability of cookies made from chickpea addition to white wheat or whole wheat flour compared to gluten‐free amaranth or buckwheat flour

The effects of chickpea addition (0–100%) on the physical properties and sensory attributes of cookies based on white or whole wheat compared to gluten‐free amaranth or buckwheat flour were studied. The physical properties determined were spread factor, colour and hardness. Sensory evaluation was conducted for colour, taste, texture and overall impression. Chickpea addition decreased the lightness in white wheat cookies while increasing it in whole wheat and amaranth cookies and it significantly increased yellowness in all cookies. Spread factor of the gluten‐free cookies was reduced by chickpea addition and hardness was increased in white wheat and buckwheat cookies, and decreased in whole wheat and amaranth cookies. Sensory evaluation demonstrated that chickpea addition increased the acceptability of all cookies, in particular of the gluten‐free cookies. Optimal levels of chickpea addition were 20–40% in wheat cookies and 60–80% for amaranth and buckwheat cookies.     

Effects of barley flour and barley protein isolate on chemical, functional, nutritional and biological properties of Pita bread

This study was carried out to investigate the effects of fortification of wheat flour with barley flour (BF) and barley protein isolate (BPI) at three levels; 5, 10 and 15% levels on the chemical composition, nutritional evaluation and biological properties of pita bread. Proteins fractions such as globulin, prolamin, glutelin-1 and glutelin-2 as well as protein isolates were extracted from barley flour and evaluated for protein yield, chemical composition and nutritional quality. Highest yield and essential amino acids contents were obtained in barley protein isolate. SDS-PAGE gels electrophoresis indicated that fortified wheat flour with BPI and BF consists of proteins coming from wheat flour and barley proteins. The contents of essential limiting amino acids in bread were increased from 1.38 to 3.10 g/100 g for lysine and from 0.86 to 1.73 g/100 g for methionine as the ratio of fortification with BF and BPI increased from 0 to 15%. The highest content of total phenolics, antioxidant activity, and inhibitory activity for both angiotensin converting enzyme (ACE) and α-amylase were found in fortified bread with BPI at 15%. Results indicated that bread made from fortification of wheat flour with BF and BPI at 15% showed superior chemical, physico-chemical, nutritional and biological properties.     

Utilization of germinated and heat-moisture treated brown rices in sugar-snap cookies

Utilization of germinated brown rice (GBR) in sugar-snap cookies and effect of heat-moisture treatment of the GBR were investigated. Brown rice was germinated at 30 °C for 48 h and then a heat-moisture treatment was conducted for the moistened GBR (17 g/100 g moisture content) at 100 °C for 4 h. Sugar-snap cookies were prepared with white rice, brown rice, GBR and the treated GBR flours, as substitutes for wheat flour (30–100 g/100 g). All cookies containing rice flours, regardless of germination and heat-moisture treatment, required significantly less force to compress than did the wheat flour cookie, and this softening effect was increased as the level of rice flour substitution increased. The cookies made with the GBR flour displayed inferior physical characteristics compared to those with wheat flour, but the cookies containing the treated GBR flour showed improved physical properties with lower moisture content and higher spread factor than those containing untreated GBR flour. The cookies containing the treated GBR flours showed relatively a low degree of firming during the ambient storage. The overall results showed that the cookies with acceptable quality and improved nutrition could be prepared by partial or complete replacement of wheat flour with the heat-moisture treated GBR flour.     

天然抗氧化剂抑制曲奇中丙烯酰胺含量的研究

采用天然抗氧化剂荞麦、孜然和黑种草籽抑制曲奇中丙烯酰胺的生成。研究这3种配料对面粉中丙烯酰胺前体的含量、面粉和曲奇抗氧化的特性、曲奇烘焙特性和丙烯酰胺含量的影响,结果表明：这3种配料组合都可以增加面粉中还原糖和天门冬酰胺的含量,也可以增加面粉和曲奇的抗氧化特性(包括总酚类物质、DPPH自由基清除能力和ABTS+·的抑制能力)。当所添加的天然抗氧化剂含量增加时,曲奇颜色变深,表现为更低的L*,a*和b*值;荞麦和孜然的引入会增加曲奇中的水分含量,而黑种草籽则相反。所有天然抗氧化剂组合都可以增加曲奇的延展率。未添加天然抗氧化剂的曲奇中丙烯酰胺的含量为361.2μg/kg,而单独添加15%荞麦、15%孜然和15%黑种草籽使得曲奇中丙烯酰胺的含量分别降低了20.2%、67.6%和75.9%。感官分析结果表明：富含天然抗氧化剂的曲奇都可以被人们所喜欢。     

The influence of α-amylase supplementation, γ-irradiation (60Co) as well as long time of storage of wheat grain on flour technological properties

The varieties of winter wheat, Aria and Beta, were studied. The Aria variety was stored for the period of four years. The part of wheat grain from Beta variety was irradiated with γ rays (⁶⁰Co). In extracts from wheat kernels and flour protein content, total α- and β-amylolytic activity as well as α-amylolytic activity were determined. α-amylases of native and fungal origin were added to the flour obtained from samples of stored wheat kernels (Aria), irradiated and non-irradiated (Beta). Consequently native α-amylase activity of flour increased by 25 % and 50% respectively. Extensive technological estimation of grain and flour with amylase supplements was carried out. The study included: sedimentation analysis, falling number test, milling experiment, farinogram and extensogram analyses, measurement of the degree of damaged starch and flour colour, as well as baking experiment. The obtained experimental loaves of bread were tested for their ability to remain fresh. It was found out that the stored grain flour was characterized by the highest α -amylolytic activity and the lowest falling number value, whereas the irradiated grain flour showed the highest degree of starch damage and water absorption. When α-amylase supplementation to doughs wasn't accompanied either by irradiation or storage of grain, it definitely changed their physical properties for the worse. The negative influence of native α-amylases appeared to be less significant than that of fungal α-amylases. The positive influence of α-amylase supplementations, especially of those increasing by 25 % the native α-amylolytic activity of flour on volume, and freshness of loaves of bread was observed.     

Effect of Wheat Bran,Coarse Wheat Flour,and Rice Flour on the Instrumental Texture of Cookies

Abstract

The effect of replacing wheat flour with wheat bran (0–10%), coarse wheat flour (0–20%) or with rice flour (0–20%) on the quality of cookies was studied. The cookie dough was subjected to Instrumental Texture Profile Analysis in order to determine dough cohesiveness and adhesiveness. After baking spread factor, puncture force and fracture strength of the cookies was also determined. The control dough had cohesiveness and adhesiveness values of 0.279 and 13.6 N s whereas control cookies had puncture force and fracture strength of 90.84 N and 100.16 N. Wheat bran increased dough cohesiveness and adhesiveness whereas coarse wheat flour had the opposite effect. Increasing levels of rice flour decreased cohesiveness but increased adhesiveness. Wheat bran and rice flour lowered the spread factor where as coarse wheat flour increased spread factor. Coarse wheat flour and rice flour lowered the fracture strength where as wheat bran increased fracture strength. Sensory evaluation revealed that increasing levels of wheat bran lowered the overall acceptability whereas rice flour and coarse wheat flour improved sensory scores.     

Antioxidant capacity and polyphenolic content of high-protein lupin products

In order to produce high protein lupin products, α-galactoside extraction from Lupinus angustifolius cv. Troll and cv. Emir and Lupinus albus cv. Multolupa, and protein isolation from L. albus cv. Multolupa were carried out. Trolox equivalent antioxidant capacity (TEAC), DPPH radical-scavenging activity (DPPH-RSA), peroxyl radical-trapping capacity (PRTC), superoxide dismutase-like activity (SOD-like activity), total phenolic compounds (TPC) and total flavonoids were determined in lupin products. In L. angustifolius cv. Troll, L. angustifolius cv. Emir and L. albus cv. Multolupa α-galactoside-low flours, the TEAC and DPPH-RSA decreased (43%, 38%; 73%, 82%; 77%, 38%, respectively). PRTC decreased in L. angustifolius cv. Troll and L. albus cv. Multolupa α-galactoside-low flours (13% and 98%, respectively), while in those of L. angustifolius cv. Emir, PRTC increased (25%). SOD-like activity and TPC were also affected by α-galactoside extraction and reductions of 30–52% and 38–56%, respectively, were observed. The protein isolate of L. albus cv. Multolupa presented lower TEAC (24%), a similar level of PRTC and twice higher level of DPPH-RSA than did α-galactoside-low Multolupa flour. Moreover, the SOD-like activity of lupin protein isolate was sharply reduced but the TPC content was 24% higher. The technological procedures, α-galactoside extraction and protein isolation, provide high protein lupin products but with lower antioxidant capacity and total phenolic compounds than the raw seeds, although the levels of antioxidant capacity of these lupin products resemble those of cereals.     

Effect of incorporation of amaranth flour on the quality of cookies

Studies were carried out on composite flour cookies by incorporating amaranth seed (Amaranthus gangeticus) flour, which is rich in protein at 0–35% levels, showed a small reduction in water absorption capacity from 58.0 to 56.5%, a considerable reduction in Farinograph stability from 3.0 to 1.5 min and increase in the mixing tolerance index from 40 to 120 BU of the dough. The pasting characteristics of the blends using rapid visco analyser (RVA) showed a reduction in peak viscosity, cold paste viscosity as well as break down viscosity from 261 to 192, 204 to 153 and 151 to 108 RVU, respectively. Incorporation of amaranth flour improved the colour of the cookies from pale cream to golden brown. The cookies became crispier which is evident from the reduction in the breaking strength value from 6.2 to 4.02 kg. Considering the colour, taste, flavour, surface appearance of the cookies, 25% incorporation of amaranth flour was found to be optimum. By the addition of glycerol mono stearate (GMS) and soy lecithin in combination at 0.5% level, the quality of the cookies further improved.