Estimating crop chlorophyll content with hyperspectral vegetation indices and the hybrid inversion method

A hybrid inversion method was developed to estimate the leaf chlorophyll content (LCC) and canopy chlorophyll content (CCC) of crops. Fifty hyperspectral vegetation indices (VIs), such as the photochemical reflectance index (PRI) and canopy chlorophyll index (CCI), were compared to identify the appropriate VIs for crop LCC and CCC inversion. The hybrid inversion models were then generated from different modelling methods, including the curve-fitting and least squares support vector regression (LS-SVR) and random forest regression (RFR) algorithms, by using simulated Compact High Resolution Imaging Spectrometer (CHRIS) datasets that were generated by a radiative transfer model. Finally, the remote-sensing mapping of a CHRIS image was completed to test the inversion accuracy. The results showed that the remote-sensing mapping of the CHRIS image yielded an accuracy of R² = 0.77 and normalized root mean squared error (NRMSE) = 17.34% for the CCC inversion, and an accuracy of only R² = 0.33 and NRMSE = 26.03% for LCC inversion, which indicates that the remote-sensing technique was more appropriate for obtaining chlorophyll content at the canopy scale (CCC) than at the leaf scale (LCC). The estimated results of various VIs and algorithms suggested that the PRI and CCI were the optimal VIs for LCC and CCC inversion, respectively, and RFR was the optimal method for modelling.     

A novel two-step method for winter wheat-leaf chlorophyll content estimation using a hyperspectral vegetation index

Remote sensing estimation of leaf chlorophyll content is of importance to crop nutrition diagnosis and yield assessment, yet the feasibility and stability of such estimation has not been assessed thoroughly for mixed pixels. This study analyses the influence of spectral mixing on leaf chlorophyll content estimation using canopy spectra simulated by the PROSAIL model and the spectral linear mixture concept. It is observed that the accuracy of leaf chlorophyll content estimation would be degraded for mixed pixels using the well-accepted approach of the combination of transformed chlorophyll absorption index (TCARI) and optimized soil-adjusted vegetation index (OSAVI). A two-step method was thus developed for winter wheat chlorophyll content estimation by taking into consideration the fractional vegetation cover using a look-up-table approach. The two methods were validated using ground spectra, airborne hyperspectral data and leaf chlorophyll content measured the same time over experimental winter wheat fields. Using the two-step method, the leaf chlorophyll content of the open canopy was estimated from the airborne hyperspectral imagery with a root mean square error of 5.18 μg cm^?2, which is an improvement of about 8.9% relative to the accuracy obtained using the TCARI/OSAVI ratio directly. This implies that the method proposed in this study has great potential for hyperspectral applications in agricultural management, particularly for applications before crop canopy closure.     

Calibration and validation of hyperspectral indices for the estimation of broadleaved forest leaf chlorophyll content,leaf mass per area,leaf area index and leaf canopy biomass

This article aims at finding efficient hyperspectral indices for the estimation of forest sun leaf chlorophyll content (CHL, µg cm_leaf^? 2), sun leaf mass per area (LMA, g_{dry matter} m_leaf^? 2), canopy leaf area index (LAI, m²_leaf m_soil^? 2) and leaf canopy biomass (B_leaf, g_{dry matter} m_soil^? 2). These parameters are useful inputs for forest ecosystem simulations at landscape scale. The method is based on the determination of the best vegetation indices (index form and wavelengths) using the radiative transfer model PROSAIL (formed by the newly-calibrated leaf reflectance model PROSPECT coupled with the multi-layer version of the canopy radiative transfer model SAIL). The results are tested on experimental measurements at both leaf and canopy scales. At the leaf scale, it is possible to estimate CHL with high precision using a two wavelength vegetation index after a simulation based calibration. At the leaf scale, the LMA is more difficult to estimate with indices. At the canopy scale, efficient indices were determined on a generic simulated database to estimate CHL, LMA, LAI and Bleaf in a general way. These indices were then applied to two Hyperion images (50 plots) on the Fontainebleau and Fougères forests and portable spectroradiometer measurements. They showed good results with an RMSE of 8.2 µg cm^? 2 for CHL, 9.1 g m^? 2 for LMA, 1.7 m² m^? 2 for LAI and 50.6 g m^? 2 for Bleaf. However, at the canopy scale, even if the wavelengths of the calibrated indices were accurately determined with the simulated database, the regressions between the indices and the biophysical characteristics still had to be calibrated on measurements. At the canopy scale, the best indices were: for leaf chlorophyll content: ND_chl = (ρ₉₂₅ ? ρ₇₁₀)/(ρ₉₂₅ + ρ₇₁₀), for leaf mass per area: ND_LMA = (ρ₂₂₆₀ ? ρ₁₄₉₀)/(ρ₂₂₆₀ + ρ₁₄₉₀), for leaf area index: D_LAI = ρ₁₇₂₅ ? ρ₉₇₀, and for canopy leaf biomass: ND_Bleaf = (ρ₂₁₆₀ ? ρ₁₅₄₀)/(ρ₂₁₆₀ + ρ₁₅₄₀).     

Towards universal broad leaf chlorophyll indices using PROSPECT simulated database and hyperspectral reflectance measurements

Fifty-three leaves were randomly sampled on different deciduous tree species, representing a wide range of chlorophyll contents, tree ages, and leaf structural features. Their reflectance was measured between 400 and 800 nm with a 1-nm step, and their chlorophyll content determined by extraction. A larger simulated database (11,583 spectra) was built using the PROSPECT model, in order to test, calibrate, and obtain universal indices, i.e., indices applicable to a wide range of species and leaf structure. To our knowledge, almost all leaf chlorophyll indices published in the literature since 1973 have been tested on both databases. Fourteen canonical types of indices (published ones and new ones) were identified, and their wavelengths calibrated on the simulated database as well as on the experimental database to determine the best wavelengths and, hence, the best performances in chlorophyll estimation for each index types. These indices go from simple reflectance ratios to more sophisticated indices using reflectance first derivatives (using the Savitzky and Golay method). We also tested other nondestructive methods to obtain total chlorophyll concentration: SPAD (Minolta Camera, Osaka, Japan) and neural networks. The validity of the actual PROSPECT model is challenged by our results: Important discordances are found when the indices are calculated with PROSPECT compared to experimental data, especially for some indices and wavelengths. The discordance is even greater when the indices are determined with PROSPECT and applied on the experimental database. A new calibration of PROSPECT is therefore necessary for any study aiming at using simulated spectra to determine or to calibrate indices. The “peak jump” and the multiple-peak feature observed on the first derivative of the reflectances (e.g., in the Red-Edge Inflection Point [REIP] index) has been investigated. It was shown that chlorophyll absorption alone can explain this feature. The peak jump disqualifies the REIP to be a valuable chlorophyll index. A simple modified difference ratio gave the best results among all published indices (cross-validated RMSE=2.1 μg/cm² on the experimental database). After calibration on the experimental database, modified Simple Ratio (mSR) and modified Normalized Difference (mND) indices gave the best performances (RMSECV=1.8 μg/cm² on the experimental database). The new Double Difference (DD) index, although not the best on the experimental database (RMSECV=2.9 μg/cm²), has the best results on the larger simulated database (RMSE=3.7 μg/cm²) and is expected to give good results on larger experimental databases. The best reflectance-based indices give better performances than the current commercial nondestructive device SPAD (RMSECV=4.5 μg/cm²). In this leaf-level study, the best indices are very near from each other, so that complex methods are useless: REIP-like, neural networks, and derivative-based indices are not necessary and give worst results than simpler properly chosen indices. These conclusions will certainly be different for a canopy-level study, where the derivative-based indices may perform significantly better than the other ones.     

Non-destructive estimation of wheat leaf chlorophyll content from hyperspectral measurements through analytical model inversion

Optimizing nitrogen (N) fertilization in crop production by in-season measurements of crop N status may improve fertilizer N use efficiency. Hyperspectral measurements may be used to assess crop N status indirectly by estimating leaf and canopy chlorophyll content. This study evaluated the ability of the PROSAIL canopy-level reflectance model to predict leaf chlorophyll content of spring wheat (Triticum aestivum L.) during the growth stages between pre-tillering (Zadoks Growth Stage (ZGS 15)) to booting (ZGS50). Spring wheat was grown under different N fertility rates (0–200 kg N ha^?1) in 2002. Canopy reflectance, leaf chlorophyll content, N content and leaf area index (LAI) values were measured. There was a weakly significant trend for the PROSAIL model to over-estimate LAI and under-estimate leaf chlorophyll content. To compensate for this interdependency by the model, a canopy chlorophyll content parameter (the product of leaf chlorophyll content and LAI) was calculated. The estimation accuracy for canopy chlorophyll content was generally low earlier in the growing season. This failure of the PROSAIL model to estimate leaf and canopy variables could be attributed to model sensitivity to canopy architecture. Earlier in the growing season, full canopy closure was not yet achieved, resulting in a non-homogenous canopy and strong soil background interference. The canopy chlorophyll content parameter was predicted more accurately than leaf chlorophyll content alone at booting (ZGS 45). A strong relationship between canopy chlorophyll content and canopy N content at ZGS 45 indicates that the PROSAIL model may be used as a tool to predict wheat N status from canopy reflectance measurements at booting or later.     

An evaluation of EO-1 hyperspectral Hyperion data for chlorophyll content and leaf area index estimation

Estimation of chlorophyll content and the leaf area index (LAI) using remote sensing technology is of particular use in precision agriculture. Wavelengths at the red edge of the vegetation spectrum (705 and 750 nm) were selected to test vegetation indices (VIs) using spaceborne hyperspectral Hyperion data for the estimation of chlorophyll content and LAI in different canopy structures. Thirty sites were selected for the ground data collection. The results show that chlorophyll content and LAI can be successfully estimated by VIs derived from Hyperion data with a root mean square error (RMSE) of 7.20–10.49 μg cm^?2 for chlorophyll content and 0.55–0.77 m² m^?2 for LAI. The special index derived from three bands provided the best estimation of the chlorophyll content (RMSE of 7.19 μg cm^?2 for the Modified Chlorophyll Absorption Ratio Index/Optimized Soil-Adjusted Vegetation Index (MCARI/OSAVI₇₀₅)) and LAI (RMSE of 0.55 m² m^?2 for a second form of the MCARI (MCARI2₇₀₅)). These results demonstrate the possibilities for analysing the variation in chlorophyll content and LAI using hyperspectral Hyperion data with bands from the red edge of the vegetation spectrum.     

Effects of chlorophyll fluorescence on the estimation of microphytobenthos biomass using spectral reflectance indices

The communities of benthic microalgae that form dense biofilms at the surface of aquatic sediments, or microphytobenthos, are important primary producers in estuarine intertidal flats and shallow coastal waters. The microalgal biomass present in the photic zone of the sediment is a key parameter for ecological and photophysiological studies on microphytobenthos, and has been routinely estimated using hyperspectral reflectance indices based on the chlorophyll (Chl) a red absorption peak at 675 nm, usually the Normalised Difference Vegetation Index (NDVI). This study reports that red region-based biomass indices measured on microphytobenthos biofilms can be significantly affected by the enrichment of reflected light with solar-induced Chl fluorescence emitted by the microalgae. Chl fluorescence emission peaks at 683 nm, counterbalancing the decrease in reflectance centered at 675 nm, thus causing the underestimation of NDVI. The interference of Chl fluorescence was found to be easily identified by a conspicuous double-peak feature in the 670-700 nm region of the second-derivative reflectance spectra. The fluorescence-induced NDVI underestimation was shown to be most pronounced for high surface biomass levels and low incident solar irradiance. Particular aspects of microphytobenthos biofilms, such as the increase in surface Chl fluorescence due the contribution of emission by subsurface layers, and vertical migratory responses by motile microalgae to changes in ambient light, further complicate the effects on biomass estimation using NDVI-like indices. By comparing NDVI with a fluorescence-independent biomass index for a wide range of natural light conditions, it was found that Chl fluorescence interference may cause the underestimation of microalgal biomass to reach over 25%, with errors above 10% being expected for more than half of the measuring occasions. These results indicate that the use of NDVI may compromise the correct assessment of important aspects of microphytobenthos ecology, such as the characterisation of migratory behaviour or the determination of biomass-specific productivity rates, and call for the use of alternative biomass indices, not based on the Chl a red absorption peak.     

Normalized sensitivity measures for leaf area index estimation using three-band spectral vegetation indices

A great number of spectral vegetation indices (SVIs) have been developed to estimate key biophysical parameters such as leaf area index (LAI). Considerable interest is often given to the local optimization, performance analysis and sensitivity of each spectral band and SVI for LAI estimation given that several confounding factors are present. In this regard, inclusion of shortwave infrared (SWIR) reflectance in traditionally near-infrared (NIR)-red (R)-based SVIs has played a great role for local optimization and increased sensitivity of SVIs to LAI. This study presents the enhanced and normalized sensitivity functions for evaluating (1) the sensitivity of each spectral band and SVI to LAI and (2) the generic performance analysis of empirical model to estimate LAI based on the SVIs. Several alternatives for three-band (NIR-R-SWIR) SVI modifications have been recommended and proven to be simplistic and unbiased way of local optimization.     

Needle chlorophyll content estimation through model inversion using hyperspectral data from boreal conifer forest canopies

Leaf chlorophyll content in coniferous forest canopies, a measure of stand condition, is the target of studies and models linking leaf reflectance and transmittance and canopy hyperspectral reflectance imagery. The viability of estimation of needle chlorophyll content from airborne hyperspectral optical data through inversion of linked leaf level and canopy level radiative transfer models is discussed in this paper. This study is focused on five sites of Jack Pine (Pinus banksiana Lamb.) in the Algoma Region (Canada), where field, laboratory and airborne data were collected in 1998 and 1999 campaigns. Airborne hyperspectral CASI data of 72 bands in the visible and near-infrared region and 2 m spatial resolution were collected from 20×20 m study sites of Jack Pine in 2 consecutive years. It was found that needle chlorophyll content could be estimated at the leaf level (r²=0.4) by inversion of the PROSPECT leaf model from needle reflectance and transmittance spectra collected with a special needle carrier apparatus coupled to the Li-Cor 1800 integrating sphere. The Jack Pine forest stands used for this study with LAI>2, and the high spatial resolution hyperspectral reflectance collected, allowed the use of the SPRINT canopy reflectance model coupled to PROSPECT for needle chlorophyll content estimation by model inversion. The optical index R750/R710 was used as the merit function in the numerical inversion to minimize the effect of shadows and LAI variation in the mean canopy reflectance from the 20×20 m plots. Estimates of needle pigment content from airborne hyperspectral reflectance using this linked leaf-canopy model inversion methodology showed an r²=0.4 and RMSE=8.1 μg/cm² when targeting sunlit crown pixels in Jack Pine sites with pigment content ranging between 26.8 and 56.8 μg/cm² (1570-3320 μg/g).     

Retrieval of leaf chlorophyll content in field crops using narrow-band indices: effects of leaf area index and leaf mean tilt angle

Reliable estimation of leaf chlorophyll-a and -b content (chl-a + b) at canopy scales is essential for monitoring vegetation productivity, physiological stress, and nutrient availability. To achieve this, narrow-band vegetation indices (VIs) derived from imaging spectroscopy data are commonly used. However, VIs are affected by canopy structures other than chl-a + b, such as leaf area index (LAI) and leaf mean tilt angle (MTA). In this study, we evaluated the performance of 58 VIs reported in the literature to be chl-a + b-sensitive against a unique measured set of species-specific leaf angles for six crop species in southern Finland. We created a large simulated canopy reflectance database (100,000 canopy configurations) using the physically based PROSAIL (coupling of PROSPECT and SAIL (Scattering by Arbitrarily Inclined Leaves) radiative transfer models) model. The performance of model-simulated indices was compared against airborne AISA Eagle II imaging spectroradiometer data and field-measured chl-a + b, LAI, and MTA values. In general, LAI had a positive effect on the strength of the correlation between chl-a + b and VIs while MTA had a negative effect in both measured and simulated data. Three indices (REIP (red edge inflection point), TCARI (transformed chlorophyll absorption ratio index)/OSAVI (optimized soil-adjusted vegetation index), and CTR6 (Carter indices)) showed strong correlations with chl-a + b and similar performance in model-simulated and measured data set. However, only two (TCARI/OSAVI and CTR6) were independent from LAI and MTA. We consider these two indices robust proxies of crop leaf chl-a + b.     

A shadow identification method using vegetation indices derived from hyperspectral data

Shadows in high-spatial-resolution remote-sensing images become more pronounced. The detection of shadows is an essential requirement for both detailed high-spatial land-cover classification and applications such as three-dimensional (3D) reconstruction of buildings as well as cloud removal. This article presents a method for integrating the photochemical reflectance index (PRI) and Red Edge normalized difference vegetation index (RENDVI) for shadow identification (IPRSI) using high-spatial-resolution airborne hyperspectral data. This method detects shadows by setting thresholds to the PRI and RENDVI to separate shadows from vegetated and non-vegetated areas. The proposed method outperformed the invariant colour spaces model and the object-based method in terms of shadow extraction accuracy. The overall shadow identification accuracy of the IPRSI was 88.97% with an F-score of 90.96 (81.32% with F-score 81.97 for the invariant colour spaces model and 78.02% with F-score 82.07 for the object-based method). The IPRSI is a potential method with the wide application of hyperspectral data in high spatial resolution that is increasingly easier to be obtained with the development of remote-sensing platforms (such as unmanned aerial vehicles (UAVs), small satellites, and airships).     

Estimation of foliar pigment concentration in floating macrophytes using hyperspectral vegetation indices

Foliar pigment concentrations of chlorophylls and cartenoids are important indicators of plant physiological status, photosynthesis rate, and net primary productivity. Although the utility of hyperspectral derived vegetation indices for estimating foliar pigment concentration has been documented for many vegetation types, floating macrophytes have not been assessed despite their ecological importance. This study surveyed 39 wetland species (12 floating macrophytes (FM), 8 grasses/sedges/rushes (GSR), and 19 herbs/wildflowers (HWF)) to determine whether foliar pigment concentrations could be estimated from hyperspectral reflectance. Hyperspectral reflectance of samples was recorded using an ASD FieldSpec3 Max portable spectroradiometer with the plant probe attachment or via a typical laboratory set-up. A semi-empirical relationship was established using either a linear, second-degree polynomial or logarithmic function between 13 candidate vegetation indices and chl-a, chl-b, Car, and chl-a + b pigment concentrations. Vegetation indices R-M, CI-Red, and MTCI were strongly correlated with foliar pigment concentrations using a linear fitting function. Chl-a + b and chl-b concentrations for all samples were reasonably estimated by the R-M index (R² = 0.66 and 0.64), although Chl-a and Car concentration estimates using CI-Red were weaker (R² = 0.63 and 0.51). Regression results indicate that pooled samples to estimate individual foliar pigments were less correlated than when each type of vegetation type was treated separately. For instance, chl-a + b was best estimated by CI-Red for FM (R² = 0.80), MTCI for HWF (R² = 0.77), and R-M for GSR (R² = 0.67). Although floating macrophytes feature unique adaptions to their aquatic environment, their foliar pigment concentrations and spectral signatures were comparable to other wetland vegetation types. Overall, vegetation indices that exploit the red-edge region were a reasonable compromise, having good explanatory power for estimation of foliar pigments across the sampled wetland vegetation types and with CI-Red the best suited index for floating macrophytes.     

Comparing the performance of multispectral vegetation indices and machine-learning algorithms for remote estimation of chlorophyll content: a case study in the Sundarbans mangrove forest

Optical vegetation indices (VIs) have been used to retrieve and assess biophysical variables from satellite reflectance data. These indices, however, also are sensitive to a number of confounding factors, such as canopy geometry, soil optical properties, and solar position. This suggests that VIs should be used cautiously for biophysical parameter estimation. Among biophysical variables, chlorophyll content is of particular importance as an indicator of photosynthetic activity. The goal of this study is to investigate the performance of multispectral optical VIs for chlorophyll content estimation in the world’s largest mangrove forest, the Sundarbans, and to compare these with machine-learning algorithms (MLAs). To this end, we have investigated the performance of 15 multispectral VIs and six state-of-the-art MLAs that are widely used for adaptive data fitting. The MLAs are Artificial Neural Networks (ANNs), Genetic Algorithm (GA), Gaussian Processes for Machine Learning (GPML), Kernel Ridge Regression (KRR), Locally Weighted Polynomials (LWP), and Multivariate Adaptive Regression Splines (MARS). We use an in situ data set of reflectance and chlorophyll measurements to develop and validate our models. Each MLA was evaluated 500 times with random partitions of training and validation data. Results showed that the weight optimization and term selection used within GA produce the most reliable chlorophyll content estimation. However, green normalized difference VI (GNDVI) is a simple and computationally efficient VI that produces results that are nearly as accurate as GA in terms of model fit and performance. Results also show that all methods except ANNs and MARS produce a quasi-linear relationship between spectral reflectance and chlorophyll content. Statistical transformations of GNDVI and chlorophyll content have the capability of further reducing model error.     

Application of hyperspectral vegetation indices to detect variations in high leaf area index temperate shrub thicket canopies

Accurate measurement of leaf area index (LAI), an important characteristic of plant canopies directly linked to primary production, is essential for monitoring changes in ecosystem C stocks and other ecosystem level fluxes. Direct measurement of LAI is labor intensive, impractical at large scales and does not capture seasonal or annual variations in canopy biomass. The need to monitor canopy related fluxes across landscapes makes remote sensing an attractive technique for estimating LAI. Many vegetation indices, such as Normalized Difference Vegetation Index (NDVI), tend to saturate at LAI levels > 4 although tropical and temperate forested ecosystems often exceed that threshold. Using two monospecific shrub thickets as model systems, we evaluated the potential of a variety of algorithms specifically developed to improve accuracy of LAI estimates in canopies where LAI exceeds saturation levels for other indices. We also tested the potential of indices developed to detect variations in canopy chlorophyll to estimate LAI because of the direct relationship between total canopy chlorophyll content and LAI. Indices were evaluated based on data from direct (litterfall) and indirect measurements (LAI-2000) of LAI. Relationships between results of direct and indirect ground-sampling techniques were also evaluated. For these two canopies, the indices that showed the highest potential to accurately differentiate LAI values > 4 were derivative indices based on red-edge spectral reflectance. Algorithms intended to improve accuracy at high LAI values in agricultural systems were insensitive when LAI exceeded 4 and offered little or no improvement over NDVI. Furthermore, indirect ground-sampling techniques often used to evaluate the potential of vegetation indices also saturate when LAI exceeds 4. Comparisons between hyperspectral vegetation indices and a saturated LAI value from indirect measurement may overestimate accuracy and sensitivity of some vegetation indices in high LAI communities. We recommend verification of indirect measurements of LAI with direct destructive sampling or litterfall collection, particularly in canopies with high LAI.     

Retrieval of crop chlorophyll content and leaf area index from decompressed hyperspectral data: the effects of data compression

The objective of this study is to evaluate whether the retrieval of the leaf chlorophyll content and leaf area index (LAI) for precision agriculture application from hyperspectral data is significantly affected by data compression. This analysis was carried out using the hyperspectral data sets acquired by Compact Airborne Spectrographic Imager (CASI) over corn fields at L'Acadie experimental farm (Agriculture and Agri-Food Canada) during the summer of 2000 and over corn, soybean and wheat fields at the former Greenbelt farm (Agriculture and Agri-Food Canada) in three intensive field campaigns during the summer of 2001. Leaf chlorophyll content and LAI were retrieved from the original data and the reconstructed data compressed/decompressed by the compression algorithm called Successive approximation multi-stage vector quantization (SAMVQ) at compression ratios of 20:1, 30:1, and 50:1. The retrieved products were evaluated against the ground-truth.In the retrieval of leaf chlorophyll content (the first data set), the spatial patterns were examined in all of the images created from the original and reconstructed data and were proven to be visually unchanged, as expected. The data measures R², absolute RMSE, and relative RMSE between the leaf chlorophyll content derived from the original and reconstructed data cubes, and the laboratory-measured values were calculated as well. The results show the retrieval accuracy of crop chlorophyll content is not significantly affected by SAMVQ at the compression ratios of 20:1, 30:1, and 50:1, relative to the observed uncertainties in ground truth values. In the retrieval of LAI (the second data set), qualitative and quantitative analyses were performed. The results show that the spatial and temporal patterns of the LAI images are not significantly affected by SAMVQ and the retrieval accuracies measured by the R², absolute RMSE, and relative RMSE between the ground-measured LAI and the estimated LAI are not significantly affected by the data compression either.     

Estimation of leaf and canopy water content in poplar plantations by means of hyperspectral indices and inverse modeling

This study investigates the applicability of empirical and radiative transfer models to estimate water content at leaf and landscape level. The main goal is to evaluate and compare the accuracy of these two approaches for estimating leaf water content by means of laboratory reflectance/transmittance measurements and for mapping leaf and canopy water content by using airborne Multispectral Infrared and Visible Imaging Spectrometer (MIVIS) data acquired over intensive poplar plantations (Ticino, Italy).At leaf level, we tested the performance of different spectral indices to estimate leaf equivalent water thickness (EWT) and leaf gravimetric water content (GWC) by using inverse ordinary least squares (OLS) regression, and reduced major axis (RMA) regression. The analysis showed that leaf reflectance is related to changes in EWT rather than GWC, with best results obtained by using RMA regression by exploiting the spectral index related to the continuum removed area of the 1200 nm water absorption feature with an explained variance of 61% and prediction error of 6.6%. Moreover, we inverted the PROSPECT leaf radiative transfer model to estimate leaf EWT and GWC and compared the results with those obtained by means of empirical models. The inversion of this model showed that leaf EWT can be successfully estimated with no prior information with mean relative errors of 14% and determination coefficient of 0.65. Inversion of the PROSPECT model showed some difficulties in the simultaneous estimation of leaf EWT and dry matter content, which led to large errors in GWC estimation.At landscape level with MIVIS data, we tested the performance of different spectral indices to estimate canopy water per unit ground area (EWT_canopy). We found a relative error of 20% using a continuum removed spectral index around 1200 nm. Furthermore, we used a model simulation to evaluate the possibility of applying empirical models based on appositely developed MIVIS double ratios to estimate mean leaf EWT at landscape level (). It is shown that combined indices (double ratios) yielded significant results in estimating leaf EWT at landscape level by using MIVIS data (with errors around 2.6%), indicating their potential in reducing the effects of LAI on the recorded signal. The accuracy of the empirical estimation of EWT_canopy and was finally compared with that obtained from inversion of the PROSPECT + SAILH canopy reflectance model to evaluate the potential of both methods for practical applications. A relative error of 27% was found for EWT_canopy and an overestimation of leaf with relative errors around 19%.Results arising from this remote sensing application support the robustness of hyperspectral regression indices for estimating water content at both leaf and landscape level, with lower relative errors compared to those obtained from inversion of leaf and 1D canopy radiative transfer models.     

Comparison of the sensor dependence of vegetation indices based on Hyperion and CHRIS hyperspectral data

In previous studies of the universal pattern decomposition method (UPDM), spectral shifts, which are very common in hyperspectral imaging spectrometers, were not taken into account when calculating standard spectral pattern vectors. This study evaluated the effect of spectral shifts on the sensor dependence of the vegetation index based on the UPDM (VIUPD) and 11 other vegetation indices (VIs). Spectral shifts were calculated using Gao's spectrum-matching method. The influences of smoothing techniques (moving average and Savitzky–Golay filters) on the consistency of these VIs were also evaluated and compared. Data from the typical narrowband imaging spectrometers, Hyperion and the Compact High Resolution Imaging Spectrometer (CHRIS), were chosen for the study. For all VIs, both smoothing and spectral calibration changed the consistency between Hyperion and CHRIS. Spectral calibration had a positive effect on the majority of VIs, whereas smoothing improved the performance of some VIs but decreased the consistency of others. When compared with spectral calibration and Savitzky–Golay smoothing, moving average generated greater variations within the results. Among the smoothing techniques employed, moving average smoothing exhibited a larger distortion of VI sensor dependency than that of Savitzky–Golay smoothing of the same order. VIUPD based on narrowband hyperspectral data was sensitive to spectral operations (spectral calibration and smoothing). For VIUPD, spectral calibration increased its sensor independence, whereas smoothing had a negative effect. After spectral calibration, VIUPD was more sensor independent than any other VI examined in this study.     

Nondestructive estimation of canopy chlorophyll content using Hyperion and Landsat/TM images

Remote sensing offers a nondestructive tool for the quick and precise estimation of canopy chlorophyll content that serves as an important indicator of the plant ecosystem. In this study, the canopy chlorophyll content of 26 samples in 2007 and 40 samples in 2008 of maize were nondestructively estimated by a set of vegetation indices (VIs; Normalized Difference Vegetation Index, NDVI; Green Chlorophyll Index, CI_green; modified soil adjust vegetation index, MSAVI; and Enhanced Vegetation Index, EVI) derived from the hyperspectral Hyperion and Thematic Mapper (TM) images. The PROSPECT model was used for sensitivity analysis among the indices and results indicated that CI_green had a large linear correlation with chlorophyll content ranging from 100–1000 mg m^?2. EVI showed a moderate ability in avoiding saturation and reached a saturation of chlorophyll content above 600 mg m^?2. Both of the other two indices, MSAVI and NDVI, showed a clear saturation at chlorophyll content of 400 mg m^?2, which demonstrated they may be inappropriate for chlorophyll interpretation at high values. A validation study was also conducted with satellite observations (Hyperion and TM) and in-situ measurements of chlorophyll content in maize. Results indicated that canopy chlorophyll content can be remotely evaluated by VIs with r ² ranging from the lowest of 0.73 for NDVI to the highest of 0.86 for CI_green. EVI had a greater precision (r ²=0.81) than MASVI (r ²=0.75) in canopy chlorophyll content estimation. The results agreed well with the sensitivity study and will be helpful in developing future models for canopy chlorophyll evaluation.     

Combining vegetation indices, constrained ordination and fuzzy classification for mapping semi-natural vegetation units from hyperspectral imagery

Vegetation mapping of plant communities at fine spatial scales is increasingly supported by remote sensing technology. However, combining ecological ground truth information and remote sensing datasets for mapping approaches is complicated by the complexity of ecological datasets. In this study, we present a new approach that uses high spatial resolution hyperspectral datasets to map vegetation units of a semiarid rangeland in Central Namibia. Field vegetation surveys provide the input to the workflow presented in this study. The collected data were classified by hierarchical cluster analysis into seven vegetation units that reflect different ecological states occurring in the study area. Spectral indices covering vegetation and soil characteristics were calculated from hyperspectral remote sensing imagery and used as environmental variables in a constrained ordination by applying redundancy analysis (RDA). The resulting statistical relationships between vegetation data and spectral indices were transferred into images of ordination axes, which were subsequently used in a supervised fuzzy c-means classification approach relying on a k-NN distance metric. Membership images for each vegetation unit as well as a confusion image of the classification result allowed a sound ecological interpretation of the resulting hard classification map. Classification results were validated with two independent reference datasets. For an internal and external validation dataset, overall accuracy reached 98% and 64% with kappa values of 0.98 and 0.53, respectively. Critical steps during the mapping workflow were highlighted and compared with similar mapping approaches.