Structure, contents, and distribution of roof rat (Rattus rattus) burrows in a rain forest.

Examined roof rat burrows in a 50-acre area of rain forest on the island of Oahu, Hawaii. All burrows were constructed on sloping ground and were spaced farther apart from one another than were other sites of digging activity. Few burrows contained nest material or food, but clusters of opened macadamia and kukui nuts were found at feeding stations that were assumed to protect the animal from predation while it was eating. Comparisons of the present data with those from several studies of Rattus norvegicus indicate that the burrows of both species are similar in overall construction and design. (30 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Some aspects of Phlebotomus papatasi (Scopoli) in greater Cairo, Egypt

Exogenous and indigenous cases of zoonotic cutaneous leishmaniasis are reported in several Egyptian Governorates particularly those in the Nile Delta, Phlebotomus paptasi (Scopoli) is the only proven insect vector. This paper was intended to study the seasonal abundance, nocturnal activity and breeding sites as well as other relevant behaviour aiming to throw some light on this insect vector. The results showed that: (a) the seasonal activity started in April and ended in November or beginning of December. (b) female outnumbered male indoors (7.4:1) and V.V. outdoors (0.14:1). (c) blood fed females were 97.7% indoors and 29.4% outdoors. (d) the nocturnal activity ranged between 6 p.m. to 6 a.m. indoors and 8 p.m. to 6 a.m. outdoors. (e) the immature stages of Phlebotomus were successfully recovered from rodent burrows and poultry sheds.     

Parasitological and clinical studies on human scabies in Cairo

This study is a parasitological and clinical study on human scabies. This study was carried out on 100 patients attending the Dermatology Outpatient Clinic at Ain Shams University Hospitals, including 54 males and 46 females. All patients were subjected to detailed history taking, complete dermatological examination including Skin Scraping Test and Burrow Ink Test. Younger patients attended the dermatology clinic earlier than older patients, who usually delayed their visits until complications occurred. Scratching, erythematous papules and secondary infected lesions were the commonest lesions. The hands, wrist and external genitalia were the most frequently affected sites while the feet, ankles, knees and back were the least affected. Burrows could only be detected in 40% of patients. The most frequent sites were the web spaces, external genitalia, and finger sides. Burrow Ink Test was positive among 85% of patients with burrows while mites could be identified by Skin Scraping Test in 55% of patients. The most frequent clinical manifestations in the parasitologically positive patients were itching, burrows, and papules, and the most frequent sites were the web spaces, and the finger sides. Multiple sites affection was the characteristic feature among the preschool age children who represented 14% of cases.     

Burrows of wild and albino rats: Effects of domestication, outdoor raising, age, experience, and maternal state.

Burrows dug by Sprague-Dawley albino rats were compared with those of wild Norway rats in an outdoor pen and in laboratory observation chambers in 5 experiments. In terms of measurements, configurations, or sequential development, burrows were indistinguishable in wild and domestic rats. Burrowing for both wild and domestic Ss was not affected by raising in outdoor burrows, by availability of nesting material, or by pregnancy. Prior experience in burrowing did make it more efficient in a 2nd trial, suggesting that learning may have a limited role in what appears to be a behavior with a strong genetic component. Feralization of domestic rats in the outdoor pen was especially productive in answering claims of degeneracy in these Ss: Albino rats were hardy throughout climatic extremes, maintained a stable population for 2 yrs, constructed and lived in burrows, and showed a variety of wild-type behaviors. (51 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The relative importance of global and local landmarks in navigation by Columbian ground squirrels (Spermophilus columbianus).

In order to survive, small burrowing mammals need to remember the locations of escape burrows. Therefore, it is important to know what types of landmarks are used to aid navigation in the wild. The author tested the ability of free-ranging Columbian ground squirrels (Spermophilus columbianus) to locate escape burrows when local (e.g., vegetation pattern, local relief), global (e.g., forest edge, mountain outline), or both types of landmarks were obstructed. Results suggest that squirrels need both local and global landmarks of the environment for successful navigation, and that the upper portion of the horizon is especially important for orientation. Moreover, the lack of information from one type of landmark (local or global) cannot be completely compensated by the other type. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Laboratory observations on the function of tongue extrusion in the desert iguana (Dipsosaurus dorsalis).

In this article, I examine rates of tongue extrusion in Dipsosaurus dorsalis to begin identification of the types of chemical information important for this species' survival and reproductive success. Increases in rates of tongue extrusion were observed in response to handling and novelty and to sand containing odors of kangaroo rats. The desert iguanas in this study did not increase their rates of tongue extrusion in response to sand containing the odors of unfamiliar conspecifics or the western whiptail lizard. Desert iguanas inhabit burrows constructed by kangaroo rats, and it is suggested that chemical information plays an important role in helping desert iguanas select appropriate burrows. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A relationship between proteinase activity and clinical parameters in the treatment of periodontal disease

The objective of this research was to determine the effectiveness of a biochemical assay which measures proteolytic enzyme activity in gingival crevicular fluid (GCF) and to relate this enzyme activity to clinical parameters traditionally utilized for periodontitis detection. A clinical trial was conducted on 8 periodontitis subjects with > or =4 sites exhibiting a loss of attachment of > or =5 mm and probing depths of > or =5 mm with bleeding on probing. On each subject, a plaque index was performed, followed by GCF sampling at those sites which exhibited a loss of attachment and probing depths. GCF was analyzed for activity against benzoyl-L-arginine-p-nitroanilide in the presence (BAPNA w/gly-gly) and the absence (BAPNA w/o gly-gly) of glycyl-glycine and against MeOSuc-Ala-Ala-Pro-Val-pNA and Suc-Ala-Ala-Pro-Phe-pNA for neutrophil serine proteinases activity (elastase and cathepsin G, respectively). Subsequently, a gingival index was performed, attachment levels and probing depths were recorded using a constant force probe with bleeding on probing being noted. A split-mouth design was employed and half mouths were randomly assigned to the following treatment groups: group A, half of the mouth received scaling/root planing and polishing: group B, half of the mouth received no treatment (control). Subjects were treated, then instructed on toothbrushing and interdental cleaning. After 4 weeks, subjects returned to receive a plaque index; GCF sampling, gingival index, attachment levels, probing depths and bleeding on probing as described above. Using a paired Student t-test, the findings suggest that BAPNA w/gly-gly was significantly less in treatment sites than in non-treated control sites (p=0.05). No such correlation was found for other activities, including neutrophil serine proteinases which were shown to occur in GCF in free, proteolytically active forms. In addition, significant treatment effects were detected for probing depths (p= 0.03) which reduced by 1.3 mm and attachment levels (p=0.02) which gained 0.7 mm. The reduction of P. gingivalis from treated periodontitis sites as detected by a significant decrease in BAPNA w/ gly-gly may prove to be a valuable marker for periodontal disease activity.     

The in vitro uptake of albumin by adult Haemonchus contortus is altered by extracorporeal digestion

FITC (fluorescein isothiocyanate)-labeled albumin was used as a substrate to further characterize the digestion of host blood proteins by adult Haemonchus contortus. Isolated H. contortus intestine degraded FITC-albumin; degradation was completely inhibited by E-64, a specific inhibitor of cysteine proteases. The in vitro uptake and degradation of FITC-albumin by parasites was also evaluated. Uptake of fluorescence was demonstrated; greater than 50% of the fluorescence was associated with degraded FITC-albumin. However, both the uptake and degradation of FITC-albumin were reduced by about 85%. Additionally, when parasites were incubated in the presence of specific inhibitors of cysteine proteases, degradation of FITC-albumin was shown to occur rapidly in the media during in vitro incubation. This degradation was blocked completely by the presence of cysteine protease inhibitors. These results indicate that the rapid extracorporeal digestion of FITC-albumin (mediated by secreted cysteine proteases) produce relatively high concentrations of low molecular weight FITC-labeled fragments and alters the kinetics of uptake of fluorescence by the parasite. These fragments rather than FITC-albumin are rapidly taken up by the parasites. Thus in vitro demonstration of a definitive role for the cysteine proteases as intestinal digestive enzymes in H. contortus is compromised by the enzyme's extracorporeal activity. This extracorporeal cysteine protease activity may represent the enzyme's predominant activity.     

The effects of localized inactivation of somatosensory cortex, area 3a, on area 2 in cats

In cats and primates, area 3a of the somatosensory cortex is the primary recipient of proprioceptive input (Phillips et al., 1971). Neurons in area 3a project to area 2 (Pons and Kaas, 1986; Porter, 1991), where somatic input relayed from the cortex and the thalamus may be integrated (Iwamura and Tanaka, 1978a,c). The goal of the present study was to determine the effects of area 3a input on neuronal activity in area 2 of cats. Extracellular recording techniques were used to identify neurons in area 2 that responded to deep stimulation of the contralateral forepaw. Neurons in area 3a that responded to the same receptive field modality and location as those in area 2 were also isolated. Single-unit or multiunit responses and evoked potentials to electrical stimulation of the shared peripheral receptive field and spontaneous activity were recorded from areas 2 and 3a. Lidocaine, a local anesthetic, was injected at the area 3a recording site to block neuronal activity. Spontaneous activity and receptive fields were abolished and evoked potentials were considerably diminished at the injection site, immediately after lidocaine was administered. Changes in unit responses, spontaneous activity, and evoked potentials in area 2 were monitored following inactivation of the somatotopically "matched" site in area 3a. Unit activity was recorded at 15 matched sites. In area 2, changes in unit responses to the peripheral stimulation and/or in spontaneous activity were observed at most of the recording sites following inactivation of area 3a. Spontaneous activity rates changed at 63% of the sites (mean change = 85%). Unit responses to the peripheral stimulation changed at 57% of the recording sites (mean change = 47%). The remaining sites in area 2 did not show lidocaine-induced changes. These sites may not have been connected with the matched sites in area 3a. Spontaneous activity and unit responses were not always similarly altered at a given site; sometimes one increased while the other decreased. Decreases in unit responses and spontaneous activity following inactivation of area 3a input were the predominant effects, indicating that area 3a has a facilitatory effect on neuronal activity observed in some regions of area 2. However, increases in neuronal activity at some sites indicated that the effects of area 3a input on area 2 are nonuniform. Evoked potentials were recorded at 19 matched sites, before and after injection of lidocaine. Evoked potentials also changed at some area 2 recording sites following area 3a inactivation.(ABSTRACT TRUNCATED AT 400 WORDS)     

Entomological and rodent surveillance of suspected plague foci in agro-environmental and feral biotopes of a few districts in Maharashtra and Gujarat states of India

Studies carried out on entomological and rodent surveillance in agroclimatic and feral biotopes of five districts of Maharashtra and two districts of Gujarat revealed that the terrain features of the seven districts surveyed were conducive to wild rodent species, Tatera indica, a natural reservoir of plague and vector flea species, Xenopsylla cheopis. A total of 214 Tatera indica and three Bandicoota bengalensis were collected from burrows by the digging method and 89 rat fleas were retrieved. The flea index calculated ranged from 0.26 to 1.0 in different districts. The examination of blood serum samples, contact tissue impression smears and tissue organs (heart, lung, liver and spleen) of these wild rodents did not reveal any evidence of plague activity. The natural harbourages of Tatera indica were found to be elevated land or raised margins of agricultural fields or barren land with thorny bushes/xerophytic plants and parthenium grass. Agricultural fields having wet soil were found to be the preferred harbourages of Bandicoota bengalensis. Movement of wild rodents toward human settlement was observed to be the common phenomenon during the harvesting season which may lead to the creation of favorable conditions for plague outbreaks.     

Studies on the vectors of leishmaniasis in Kenya: phlebotomine sandflies of Sandai location, Baringo district

Adults of phlebotomine sandflies were collected from natural and man-made habitats in Sandai area of Baringo district using sticky and CDC light traps. Three sandfly species belongings to the Phlebotomus genus and eight species belonging to the Sergentomyia genus were recorded in the area. These flies fall into "perennial" group as they appeared to breed throughout the year. During the dry season there was an increase of sandfly densities in animal burrows and termite mounds, the main sandfly breeding places. Tree holes and human habitations constituted the major sandfly resting places during the wet season. Sandflies of the Phlebotomus genus were more collected in termite mounds and inside houses using CDC light traps, whereas in animal burrows they were more collected by sticky traps than by CDC traps. Sandflies of the Sergentomyia genus were equally collected by sticky traps and CDC light traps. However, in animal burrows and in human habitations, sandflies were more collected by CDC light traps than by sticky traps. On statistical analysis, CDC light traps seemed to be effective inside houses and in animal burrows. In termite mounds both traps yielded uniform results.     

Microinfusion of cocaine into the medial preoptic area or nucleus accumbens transiently impairs maternal behavior in the rat.

Cocaine was microinfused bilaterally (50 μg/0.5 μl/side) into the medial preoptic area (MPOA) or nucleus accumbens (NA), 2 regions within the rat brain neural circuit known to mediate maternal behavior (MB). Additionally, 2 sites not involved in this neural circuit, the dorsal striatum and dorsal medial hippocampus, were used as control sites. Microinfusion of cocaine into the MPOA or NA impaired MB, whereas infusion into the control sites did not. MB impairment was not temporally coincident with the increased locomotor activity, also documented after cocaine infusion into the MPOA or NA, arguing strongly that impaired MB is a direct, specific effect of cocaine in these areas, not a derivative of increased motor activity. This is the first demonstration that cocaine action on single central nervous system (CNS) sites can impair MB to the same extent as systemic injections. Thus, cocaine's simultaneous effect on multiple CNS sites is not required for MB impairment. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Vincristine saturation of cellular binding sites and its cytotoxic activity in human lymphoblastic leukemia cells: mechanism of inoculum effect

Vincristine (VCR) is an active agent in the treatment of acute lymphoblastic leukemia (ALL). We evaluated the relationship between the cytotoxic activity of VCR and the degree of VCR saturation of cellular drug binding sites, using the MOLT-3 ALL cell line. When MOLT-3 cells at a density of 1 x 10(6) or 1 x 10(8) cells/mL of pH-controlled medium were exposed to VCR for 1 hr, its cytotoxic activity on cells at high density was 10-fold less than on cells at low density (inoculum effect). The number of VCR binding sites measured by Scatchard analysis was 9.25 x 10(6)/cell. At high cell density, the saturation of VCR binding sites was one log order less than that at low density. Irrespective of cell density, curves of cell-kill versus the degree of VCR saturation of the cellular binding sites overlapped each other. Minimal cytotoxic activity was observed at 0.3% VCR saturation, and nearly maximal cytotoxic activity occurred at about 25% saturation, with the Ic50 at about 4% saturation. These data show that the VCR-induced cell-kill effect is dependent on the degree of saturation of VCR binding sites rather than on the extracellular VCR concentration. The lesser cell-kill on cells at high density can be explained by the lack of drug molecules to sufficiently saturate cellular binding sites. This phenomenon may be responsible, at least in part, for the poor chemotherapeutic outcome of ALL patients with high leukocyte counts at presentation.     

N-glycosylation is requisite for the enzyme activity and Golgi retention of N-acetylglucosaminyltransferase III

UDP-N-acetylglucosamine: beta-D-mannoside beta-1,4N-acetylglucosaminyltransferase III (GnT-III, EC 2.4.1.144) is a glycoprotein involved in the biosynthesis of N-linked oligosaccharides. Rat GnT-III contains three potential N-glycosylation sites, which have been predicted to be Asn243, Asn261, and Asn399. To study the roles of N-glycosylation in the GnT-III function, rat GnT-III was expressed in COS-1 cells under tunicamycin or castanospermine treatment. The tunicamycin-treated GnT-III, which was not N-glycosylated, had almost no activity. The castanospermine-treated GnT-III was not localized in the Golgi, but glucosylation did not affect its activity. To clarify the role of individual N-glycosylations, we obtained a series of mutant cDNAs in which some or all of the potential glycosylation sites were eliminated by site-directed mutagenesis, and expressed them in COS-1 cells. All the mutants exhibited lower enzyme activity than the wild-type, but deglycosylation at individual sites had different effects on the enzyme activity. The deglycosylation at Asn243 or Asn261 was more effective on the activity than that at Asn399. The enzyme activity decreased as the number of glycosylation sites decreased. The null glycosylation mutant had no activity, corresponding to the case of tunicamycin-treated wild-type GnT-III. Kinetic analysis revealed that the deglycosylation at Asn243 or Asn261 resulted in slightly lower affinity for the donor substrate, but the other mutation did not significantly change the K(m) value for either the donor or acceptor. None of the mutant GnT-IIIs showed perinuclear localization or Golgi retention, that was observed for the wild-type protein. This is the first demonstration that the glycosyltransferase localized in the Golgi apparatus requires N-glycosylation for its activity and retention.     

Interactions between lonidamine and beta- or hydroxypropyl-beta-cyclodextrin

Equilibrium dialysis and Scatchard plots were used to establish that human and rabbit paraoxonases both have two calcium binding sites. Independent-site and stepwise constant analyses were used to calculate a higher affinity site (Kd1) of 3.6 +/- 0.9 x 10(-7) M for human A paraoxonase, and 1.4 +/- 0.5 x 10(-8) M for rabbit paraoxonase, and a lower affinity site (Kd2) of 6.6 +/- 1.2 x 10(-6) M for human A paraoxonase, and 5.3 +/- 0.94 x 10(-6) M for rabbit paraoxonase. In both species, the higher affinity sites were found to be essential to maintain hydrolytic activity; complete removal of calcium led to irreversible inactivation. The lower affinity sites were required for catalytic activity, and their binding of calcium was reversible. Experimentally estimated values of Kd2 based on the concentration of calcium required to obtain half the maximum enzymatic activity were 3 microM for human A and B paraoxonases, and also in the order of 3 microM for rabbit paraoxonase, using three different substrates. Calcium was the only metal found that protects against denaturation and also confers hydrolytic activity with these two mammalian paraoxonases.     

Monoamine neurotoxins: selective and delayed effects on behavior in colonies of laboratory rats

Male laboratory rats were raised in two colonies, each of 27 rats, and then given intraventricular injections of the norepinephrine neurotoxin 6-hydroxydopamine (6-OHDA) or the serotonin neurotoxin 5,6-dihydroxytryptamine (5,6-DHT) or saline. They were then returned to their enclosure and behavior during the next 50 days was observed. Shortly after neurotoxin injections the 6-OHDA rats spent more time in the burrows than controls and when out were inactive. The 5,6-DHT rats in contrast spent more time in the open than controls, ran more in activity wheels, approached humans, and fought more. Fighting, mounting, and hoarding in the colony gradually increased for 25 days; during this time the status of the 6-OHDA animals fell progressively whereas the 5,6-DHT animals increased in dominance. Social behavior returned to more normal levels after 50 days. Several successive stages of behavioral alterations occur following neurotoxin injections.     

Appearance of granulocyte-macrophage colony-stimulating factor activity at allergen-challenged cutaneous late-phase reaction sites

The cytokines IL-3 and granulocyte-macrophage CSF (GM-CSF) activate and/or prime monocytes, basophils, and eosinophils for a number of proinflammatory events in vitro. It was hypothesized that IL-3 and GM-CSF might also participate in the local inflammatory cascades that occur at cutaneous blister sites after Ag challenge in vivo. The M-07e megakaryocytic leukemia cell line, which proliferates in response to IL-3 or GM-CSF, was used to determine whether these cytokines were present in fluids derived after Ag challenge in the cutaneous blister chamber model. Fluids from blister chambers after either Ag (timothy grass, orchard grass, or ragweed) or vehicle control challenge were collected hourly for 12 h from nine patients with allergic rhinitis. Cytokine (IL-3/GM-CSF) activity was modestly elevated at 4 h after Ag challenge compared to control with the median of maximal proliferation 4% (range, 2 to 22%) vs 2% (range, 1 to 14%), respectively (Ag vs control, p < 0.03). Activity peaked at 7 h (Ag = 10%, range 1 to 12%, vs control = 1%, range 1 to 9%, p < 0.02) and then steadily declined. No increase in cytokine activity over control was seen in Ag-challenged nonatopics (n = 5, p = NS), indicating that release did not result from a nonspecific effect of the Ag solution. Neutralization of cytokine bioactivity in pooled late phase reaction (LPR) fluids from h 4 to 12 (n = 5) with anti-IL-3, anti-GM-CSF, or both antisera revealed that the majority of the activity was GM-CSF. To better quantify cytokine levels, pooled LPR fluids prepared from an additional 11 subjects were concentration-dialyzed (10x) and tested for cytokine activity. Pooled fluids from Ag-challenged sites contained a median of 625 pg/ml (range, 30 to 1250 pg/ml) GM-CSF equivalents, whereas those from the vehicle control-challenged sites contained a median 30 pg/ml (range, 30 to 300 pg/ml) GM-CSF equivalents (p < 0.004 Ag vs control groups, n = 11). Concentrated fluid from Ag- and control-challenged sites in two nonatopic subjects contained < 10 pg/ml cytokine activity. To evaluate the IL-3 and GM-CSF activity with a separate technique, ELISA were performed on separately pooled blister fluids from six atopic subjects. Although no IL-3 activity was detected after Ag challenge in these six subjects, all of them demonstrated levels of GM-CSF at Ag-challenged sites comparable to that found in the bioassay.(ABSTRACT TRUNCATED AT 400 WORDS)     

A DNA controlled-release coating for gene transfer: transfection in skeletal and cardiac muscle

In this paper we report a novel technique of DNA-polymer coating for gene transfer. A proprietary DNA polymer solution was used for thin-layer coating on a chromic gut suture as a model study. The coated sutures were characterized for physical properties such as coating thickness, mass of the DNA deposited on the suture, surface characteristics as determined by scanning electron microscopy, and in vitro DNA release characteristics under simulated physiologic conditions. The in vivo gene transfection using DNA-coated sutures was demonstrated in rat skeletal muscle and in canine atrial myocardium. A heat-stable human placental alkaline phosphatase (AP) plasmid was used as a marker gene. Incisions of 1 to 1.5 cm were made in the rat skeletal muscles or the canine atrial myocardium. The sites were closed with either the DNA-coated sutures or control sutures. Two weeks after the surgery, the tissue samples adjacent to the suture lines were retrieved and analyzed for AP activity. The DNA-coated sutures demonstrated a sustained release of the DNA under in vitro conditions, with an approximately 84% cumulative DNA release occurring in 26 days. An agarose gel electrophoresis of the DNA samples released from the suture demonstrated two bands, with the lower band corresponding to the input DNA (supercoiled). It seems that there was a partial transformation of the DNA from a supercoiled to an open circular form due to the polymer coating. The tissue sites, which received the DNA-coated sutures, demonstrated a significantly higher AP activity compared with the tissue sites that received control sutures. In the rat studies, the mean AP activity (square root of cpm/microgram protein) was 43.6 +/- 3.3 vs 20.6 +/- 2.1 (p = 0.001) at the control sites. Similarly, in the canine studies, the AP activity was 73.6 +/- 7.4 Vs 21.6 +/- 1.4 (p = 0.0009) at the control sites. Thus, our studies demonstrated a successful gene transfer using our DNA-polymer coating technique. This technique could be useful for coating sutures used in vascular and general surgery, and also for coating medical devices, such as stents, catheters, or orthopedic devices, to achieve a site-specific gene delivery.     

Immunohistopathological status of the skin in cases infested with three species of mites

This study was performed on eighty patients with clinical manifestations of scabies. Sarcoptes scabiei adult or larva was isolated from 39 patients (48.8%). Dermanyssus gallinae (red poultry mite) and Dermatophagoides sp. were isolated from 6 and 5 patients respectively. Punch biopsies were taken from the patients, stained with haematoxylin and eosin and direct immunofluorescence stain to study the immunopathological status of the skin infested with three species of mites. The results declared that the pathological changes and the immunoglobulin deposition in the skin were nearly similar regardless of the type of the causative mite with the except of the presence of burrows in Sarcoptes scabiei.