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Cor a 14, the allergenic 2S albumin from hazelnut,is highly thermostable and resistant to gastrointestinal digestion 下载免费PDF全文
Sabine Pfeifer Merima Bublin Pawel Dubiela Karin Hummel Judith Wortmann Gerhard Hofer Walter Keller Christian Radauer Karin Hoffmann‐Sommergruber 《Molecular nutrition & food research》2015,59(10):2077-2086
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Polymerase chain reaction (PCR) for detection of potentially allergenic hazelnut residues in complex food matrixes 总被引:1,自引:0,他引:1
Corylus avellana ) residues in complex food matrixes has been developed applying the polymerase chain reaction (PCR) with “hot start” and “time-release”
features. By amplifying a 182 bp- fragment of the coding deoxyribonucleic acid (cDNA) of Cor a 1, the major hazelnut allergen,
detection of even 0.001 % of hazelnut in commercial food products could be demonstrated. Results were confirmed by our previously
described sandwich-type enzyme-linked immunosorbent assay (ELISA) that quantifies potentially allergenic hazelnut protein
at trace levels.
Received: 9 November 1999 / Revised version: 9 December 1999 相似文献
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Marta Prado Jos Casqueiro Yolanda Iglesias Alberto Cepeda Jorge Barros‐Velzquez 《Journal of the science of food and agriculture》2004,84(6):505-512
A polymerase chain reaction (PCR) method was compared with a variation of the official microscopic technique (Directive 98/88/EC) for the detection in animal meals of cereals (wheat and corn) and animal parts (bone, feathers, meat, liver, fat and blood). Microscopy successfully detected animal bones in raw feeds with a sensitivity of 1 g kg?1, while the sensitivity of the PCR method was in the range of 5–10 g kg?1. Microscopy also allowed the detection of animal bones and feathers in feeds processed at 115 and 133 °C but failed to detect other animal materials. The PCR method successfully detected cereals (wheat and corn) as well as meat, bone, liver, fat and feathers after processing at 115 °C for 20 min. Heating at 133 °C under overpressure (autoclave) conditions resulted in more intense DNA fragmentation and lower DNA extractability. Nevertheless, bone and liver, as well as wheat and corn in home‐made animal meals, were successfully detected even after heating at 133 °C for 20 min. Copyright © 2004 Society of Chemical Industry 相似文献
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Luc van Dyck Amparo Pascual-Ahuir Benedicte Purnelle Andre Goffeau 《Yeast (Chichester, England)》1995,11(10):987-991
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Celina Cziepluch Elisabeth Kordes Aurora Pujol Jean-Claude Jauniaux 《Yeast (Chichester, England)》1996,12(14):1471-1474
The complete sequence of a 40 247 bp DNA segment located on the left arm of chromosome X of Saccharomyces cerevisiae has been determined and analysed. The sequence encodes the 5′ coding region of the URA2 gene and 18 open reading frames of at least 100 amino acids. Ten of these correspond to known genes, whereas eight correspond to new genes. In addition, the sequence contains a tRNA-Ala gene, a tRNA-Asp gene, a Ty4 transposable element and three delta elements. The sequence has been deposited in the EMBL databank under Accession Numbers: Z49405, Z49404, Z49403, Z49402, Z49401, Z49400, Z49399, Z49398, Z49397, Z49396, Z49394, Z49392, Z49391, Z49390, Z49389, Z49387, Z49386, Z49385. 相似文献