Effect of vitamin E supplementation on α-tocopherol and β-carotene concentrations in tissues from pasture- and grain-fed cattle

The effects of dietary vitamin E supplementation of grain-fed cattle on lipid oxidation and meat colour have been extensively investigated, but little attention has been given to pasture-fed cattle where meat is likely to contain naturally high amounts of α-tocopherol and carotenoids. In the work described, we evaluated the effects of pasture-feeding alone and with vitamin E supplementation on tissue levels of anti-oxidants and compared the findings with those obtained for grain-fed cattle with and without supplementation. Sorghum was the major component of the grained-based ration. α-Tocopherol concentrations in plasma, muscle and fat tissues of pasture-fed cattle were not affected by vitamin E supplementation (2500 IU/head/day for 132 days prior to slaughter) while those of grain-fed cattle increased significantly. The α-tocopherol concentrations in the supplemented grain-fed cattle were similar in muscle and liver to pasture-fed animals but were lower in their fat (P<0.05). The major carotenoid present in all tissues studied from pasture-fed was β-carotene and its contents in plasma, liver, fat and muscles were decreased (P<0.05) by supplementation with vitamin E. Carotenoids were essentially absent in grain-fed cattle except for small amounts in liver. The implication of this study for the meat industry is that cattle grazed on good pasture can achieve concentrations of α-tocopherol in muscles and other tissues at least as high as those obtained by supra-nutritional supplementation of grain-fed cattle with vitamin E. However, α-tocopherol supplementation of pasture-fed cattle reduced tissue concentrations of β-carotene, which would reduce carcase fat yellowness and make pasture-fed cattle more acceptable to some Asian markets.     

Meta-analysis of the effects of dietary vitamin E supplementation on α-tocopherol concentration and lipid oxidation in pork

Meta-analyses have been carried out to quantify the effect of dietary vitamin E on α-tocopherol accumulation and on lipid oxidation in porcine M. longissimus. Published results of 13 (vitamin E accumulation) and 10 (lipid oxidation) experiments respectively were used for the analyses. After a number of standardization procedures, a nonlinear relationship was found between the supplementary vitamin E and the accumulation of α-tocopherol in pork which approached a maximum value of 6.4 μg/g tissue. Pork lipid oxidation levels were described in terms of Thiobarbituric Acid Reacting Substances (TBARS) values. The statistical analysis revealed significant effect of vitamin E dose, muscle α-tocopherol concentration and supplementation time on TBARS, resulting in two prediction models for lipid oxidation. Meta-analysis has proven to be a valuable tool for combining results from previous studies to quantify the effects of dietary vitamin E. Further studies, carried out with standardized experimental protocols would be beneficial for model validation and to increase the predictive power of the derived models.     

Distribution of α-tocopherol in beef muscles following dietary α-tocopheryl acetate supplementation

The objective of this study was to determine the effect of dietary vitamin E supplementation on the distribution and concentration of α-tocopherol in beef muscles. Crossbred cattle (n=8) were selected and divided into two groups and fed diets containing 20 (basal) and 3000 mg (supplemented) α-tocopheryl acetate/head/day for 135 days prior to slaughter. Carcasses were split centrally and chilled at 4°C for 10 days. Muscles (n=16) were identified and removed from the left side of each animal and stored at -20°C until required. Mean α-tocopherol levels in muscles were significantly (P<0.05) higher in all supplemented muscles with the exception of m. infraspinatis and m. deltoidous compared to controls. Mean α-tocopherol levels in muscles from the supplemented group decreased in the order m. supraspinatis > m. psoas major > m. trapezius > m. gluteus medius > m. triceps caput brachii lumborum > m. rhomboidous > m. seratus ventralis > m. gluteobiceps > m. semitendinosus > m. semimembranosus > m. infraspinatis > m. subscapularis > m. tricepscaputbrachiilaterale > m. deltoidous > m. longissimus thorasis > m. longissimus lumborum. Significant (P<0.05) differences in α-tocopherol distribution within muscles were observed for supplemented m. psoas major and control m. seratus ventralis (highest levels in posterior ends and lowest in anterior ends) only. However, trends showed definite distribution patterns for other muscles. Levels of α-tocopherol were found to be highest in oxidative muscles (m. psoas major and m. gluteus medius) and lowest in glycolytic muscles (m. longissimus thoracis and m. longissimus lumborum) while moderate levels of α-tocopherol occurred in intermediate muscles (m. semimembranosus).     

Sensitivity to ionic strength of MgCa-enhanced ATPase activity as an index of myofibrillar ageing in beef

Modifications of MgCa-enhanced ATPase activity and its sensitivity to ionic strength were studied during the conditioning of beef Longissimus dorsi muscle, together with the changes in the mechanical properties of the myofibrillar structure assessed instrumentally by a compressive test. As the storage time increases, the ATPase activity increases at low ionic strengths whereas it decreases at higher ones. Concomitantly, we observe an increment, with storage time, in the slope of the straight line graph obtained when plotting this ATPase activity against KCl concentrations. This slope enhancement is parallel with the decrease in the maximum compressive strength. Furthermore, these two changes have been significantly correlated (P < 0·01). We conclude that the slope value which quantifies the sensitivity of the ATPase activity to ionic strength could be an accurate indicator of the degree of ageing of the myofibrillar structure and has been called the Biochemical Index of Myofibrillar Ageing (BIMA).     

Technical note: Rapid field test for the quantification of vitamin E, β-carotene,and vitamin A in whole blood and plasma of dairy cattle

Fast and easy tests for quantifying fat-soluble vitamins such as vitamin E and vitamin A, as well as β-carotene, in whole blood without a need to preprocess blood samples could facilitate assessment of the vitamin status of dairy cattle. The objective of this study was to validate a field-portable fluorometer/spectrophotometer assay for the rapid quantification of these vitamins in whole blood and plasma of dairy cows and calves. We measured the concentrations of vitamin E and β-carotene in whole blood and plasma from 28 dairy cows and 11 calves using the iCheck test (BioAnalyt GmbH, Teltow, Germany) and compared the results with the current analytical standard (HPLC) in 2 independent laboratories, one at the University of Potsdam (Germany) and at one at DSM Nutritional Products Ltd. (Kaiseraugst, Switzerland). For vitamin A, the HPLC measurements were done only in the laboratory in Germany. The whole-blood concentrations of vitamin E as determined by iCheck (blood-hematocrit-corrected) ranged from 1.82 to 4.99 mg/L in dairy cows and 0.34 to 3.40 mg/L in calves. These findings were moderately correlated (R² = 0.66) with the values assessed by HPLC in dairy cattle (cows + calves). When calves were excluded, the correlation was higher (R² = 0.961). The β-carotene and vitamin A values obtained by the reference method HPLC were highly correlated with the iCheck methods in whole blood (R² = 0.99 and 0.88, respectively). In plasma, we observed strong correlations between the concentrations assessed by iCheck and those of HPLC for vitamin E (R² = 0.97), β-carotene (R² = 0.98), and vitamin A (R² = 0.92) in dairy cattle (cows + calves). For vitamin E, β-carotene, and vitamin A, we compared the relationship between the differences obtained by the iCheck assay and the HPLC measurements, as well as the magnitude of measurements, using Bland–Altman plots to test for systematic bias. For all 3 vitamins, the differences values were not outside the 95% acceptability limits; we found no systematic error between the 2 methods for all 3 analytes.     

Effect of supplementation with vitamin D₃ on glucose production pathways in human subjects

Scope: Research reports suggest that vitamin D affects glucose and insulin metabolism; however, the exact mechanisms are unclear. ²H NMR analysis of monoacetone glucose (MAG) after tracer administration provides a non‐invasive method of profiling hepatic glucose metabolism. This study examined the effects of supplementation with vitamin D₃ on contribution of glycogenolysis to glucose production. Methods and results: Tracer administration and biofluid collections were performed with eight healthy females before and following a 4‐wk vitamin D₃ administration period. Following an overnight fast subjects ingested deuterated water and acetaminophen. Full void urine samples were collected after 4 h. ²H NMR spectra of urinary monoacetone glucose were acquired to determine the contribution of glycogenolysis to glucose production. The mean contribution of glycogenolysis to glucose production was 60±13%. Supplementation with vitamin D₃ had no effect on hepatic glucose production. Regression analysis revealed a significant relationship between carbohydrate intake and the contribution of glycogenolysis (β=0.914, p=0.004). Conclusion: In conclusion, we saw no changes in the percentage contribution of glycogenolysis following supplementation with vitamin D₃. The reproducibility of our results and the non‐invasive nature of the method highlight the potential for this method in assessing mechanistic modes of action in future nutritional interventions.     

Is rumination time an indicator of methane production in dairy cows?

As long as large-scale recording of expensive-to-measure and labor-consuming traits, such as dry matter intake (DMI) and CH₄ production (CH₄P), continues to be challenging in practical conditions, alternative traits that are already routinely recorded in dairy herds should be investigated. An ideal indicator trait must, in addition to expressing genetic variation, have a strong correlation with the trait of interest. Our aim was to estimate individual level and phenotypic correlations between rumination time (RT), CH₄P, and DMI to determine if RT could be used as an indicator trait for CH₄P and DMI. Data from 343 Danish Holstein cows were collected at the Danish Cattle Research Centre for a period of approximately 3 yr. The data set consisted of 14,890 records for DMI, 15,835 for RT, and 6,693 for CH₄P. Data were divided in primiparous cows only (PC) and all cows (MC), and then divided in lactation stage (early, mid, late, and whole lactation) to analyze the changes over lactation. Linear mixed models, including an animal effect but no pedigree, were used to estimate the correlations among traits. Phenotypic and individual level correlations between RT and both CH₄P and DMI were close to zero, regardless of lactation stage and data set (PC or MC). However, CH₄P and DMI were highly correlated, both across lactation stages and data sets. In conclusion, RT is unsuitable to be used as an indicator trait for either CH₄P or DMI. Our study failed to validate RT as a useful indicator trait for both CH₄P and DMI, but more studies with novel phenotypes can offer different approaches to select and incorporate important yet difficult to record traits into breeding goals and selection indexes.     

Vitamin D₂ impairs utilization of vitamin D₃ in high-yielding dairy cows in a cross-over supplementation regimen

Vitamin D exists in 2 forms that are important regarding vitamin D status and supply in cattle: vitamin D₂ (D₂) and vitamin D₃ (D₃). To become physiologically active, both D₂ and D₃ must undergo 25-hydroxylation in the liver. The resulting 25-hydroxyvitamin D₂ [25(OH)D₂] and 25-hydroxyvitamin D₃ [25(OH)D₃] are measured as indicators of the physiological vitamin D status of cattle. The study used 14 Danish Holstein cows housed without access to sunlight. The cows were orally administered 250 mg (1.0 × 10⁷ IU) of D₂ and D₃ in a cross-over design with 2 treatment groups and 2 study periods, rendering 4 treatments when carryover effects were taken into account: D₂ given first, D₂ given last after D₃, D₃ given first, and D₃ given last after D₂. Two weeks elapsed between the treatment in the first study period and the treatment in the second study period. Blood samples were collected 0, 3, 6, 14, 17, 20, 23, 26, 40, 48, 70, 94, 166, and 214 h after providing the oral bolus of vitamin to the cows. Comparisons between plasma levels of the metabolites D₂, D₃, 25(OH)D₂, and 25(OH)D₃ over time were made by comparing areas under the plasma concentration curves. Oral administration of D₃ increased plasma D₃ (182.6 ± 17.1 ng/mL; mean ± SEM) and 25(OH)D₃ (103.5 ± 10.0 ng/mL) more efficiently than oral administration of D₂ increased plasma D₂ (49.1 ± 32.6 ng/mL) and 25(OH)D₂ (27.9 ± 2.1 ng/mL). The D₃ given after an oral dose of D₂ was less efficient for increasing plasma concentrations of 25(OH)D₃ (61.2 ± 12.0 ng/mL) compared with D₃ given without previous D₂ administration (103.5 ± 10.0 ng/mL), whereas the plasma concentrations of D₃ itself were the same when given first (182.6 ± 17.1 ng/mL) as when given after D₂ (200.0 ± 123.9 ng/mL). The same occurred for plasma concentrations of D₂ metabolites both if D₂ was given first (49.1 ± 32.6 ng/mL) and after D₃ (54.7 ± 7.7 ng/mL). In conclusion, D₃ given after D₂ is less efficient at increasing the plasma status of 25(OH)D₃ than D₃ given without previous D₂ administration.     

Supranutritional vitamin E supplementation in pigs: Influence on subcellular deposition of α-tocopherol and on oxidative stability by conventional and derivative spectrophotometry

The influence of three levels of vitamin E (30, 200 and 1000mg kg⁻¹) in the diet of pigs on the subcellular deposition of α-tocopherol in muscle and on the oxidative stability determined by conventional and first derivative Spectrophotometry was studied. The content of α-tocopherol in m. gluteo biceps and in mitochondrial and microsomal fractions of the muscle significantly increased (p < 0.01) with increasing levels of dietary vitamin E. Concentrations of α-tocopherol in muscle, mitochondria and microsomes of pigs fed diet supplemented with 1000 mg kg⁻¹ α-tocopheryl acetate were 3.2-, 6.1- and 5.6-fold greater, respectively, than those in their counterparts from the control animals. These differences in α-tocopherol concentration in the subcellular fractions and intact muscle resulted in enhanced stability of the membranes and the tissue when exposed to iron-ascorbate induced peroxidation. When lipid oxidation in the same samples was further measured by the first derivative method, the resultant MDA-TBA values were 59–69% lower in tissue samples, 16–19% lower in mitochondria and 6–9% lower in microsomes than the conventional TBARS values.     

Colour of subcutaneous adipose tissue and M. longissimus dorsi of high index dairy and beef×dairy cattle slaughtered at two liveweights as bulls and steers

The objectives of this study were (a) to compare muscle and adipose tissue colour of male progeny of two strains of high genetic merit Friesian cows (New Zealand [NZF] and Irish [DAF]) with those of beef (Belgian Blue)×dairy (Holstein-Friesian) [BBHF] male progeny; (b) to compare bulls and steers (gender) of these genotypes and (c) to examine the effects of slaughter weight (SW) on these quality traits. Bulls (n=48) and steers (n=48) of the three genotypes were grown to nominal target liveweights of 550 kg (light) and 630 kg (heavy). Adipose tissue from the NZF genotype was more yellow (P<0.05) than from DAF or BBHF, regardless of gender or SW. For longissimus dorsi (LD) pH, bulls and heavy animals had higher pH (P<0.05) than steers or light animals, respectively, while NZF and BBHF bulls had higher pH than steers. LD muscle from the BBHF genotype had lighter colour (P<0.05) and lower haem pigments (P<0.01) than NZF or DAF progeny. There was no difference in muscle `L' value between light bulls and steers but heavy bulls had darker muscle than heavy steers. There was an interaction between genotype, gender and SW for LD redness. Thus, NZF animals were most red when slaughtered as light or heavy bulls, but there were no differences between genotypes slaughtered as light or heavy steers. These data demonstrate differences in colour of beef, especially from progeny of NZF, which produced the most yellow adipose tissue and the most red muscle tissue.     

Influence of dietary fat and vitamin E supplementation on α-tocopherol levels and fatty acid profiles in chicken muscle membranal fractions and on susceptibility to lipid peroxidation

Broiler chickens were fed a basal feed supplemented with 10% tallow or olive oil and varying levels of vitamin E (20 and 200 mg vitamin E/kg feed). The concentration of α-tocopherol in the membranes of breast and thigh muscles was significantly influenced by the α-tocopherol level in the feed (p < 0.001). Deposition of vitamin E was not influenced by the type of oil in the feed, except in the mitochondrial fraction of breast where the vitamin E concentration was higher in those fed olive oil than in those fed tallow (p < 0.05). Dietary oil influenced the fatty acid composition of the muscle membranal fractions (p < 0.001). The oxidative stability of the membranal fractions tended to increase with increasing concentrations of α-tocopherol in the membranal fractions. In conclusion, the supplementation of vitamin E appeared to enhance the stability of muscle to oxidation. Thus, incorporation of α-tocopherol into the membranes via dietary manipulation helped in stabilizing the membrane-bound lipids.     

Ability of α-tocopherol,taurine and rosemary,in combination with vitamin C,to increase the oxidative stability of beef steaks packaged in modified atmosphere

Fresh beef steaks were sprayed on the surface with vitamin C (500 ppm), taurine (50 mM), rosemary (1000 ppm) and vitamin E (100 ppm), the three latter in combination with 500 ppm of Vitamin C, packaged in modified atmosphere (70% O₂+20% CO₂+10% N₂) and stored at 1±1 °C for 29 days. Metmyoglobin formation, lipid oxidation (TBARS), instrumental colour (CIE a¹), psychrotrophic bacterial counts (PCA) and sensory discolouration and odour were determined. Results demonstrated that surface application of antioxidant combinations resulted in an effective delay of oxidative deterioration of fresh beef steaks. Shelf life was extended beyond that of control, according to evaluation of sensory attributes. Both combinations of vitamin C with either rosemary or taurine significantly (P<0.01) extended the shelf life of fresh beef steaks by about 10 days. Rosemary was the most effective in delaying oxidation processes. The combination of vitamins E and C was significantly (P<0.01) less effective than those combinations in delaying meat oxidation.     

Application of absolute qPCR as a screening method to detect illicit 17β-oestradiol administration in male cattle

It has been previously demonstrated that the progesterone receptor gene is up-regulated in the sex accessory glands of pre-pubertal and adult male bovines after 17β-oestradiol treatment. In the present study, a qualitative screening method was optimised to detect 17β-oestradiol treatment using absolute quantification by qPCR of the progesterone receptor gene to determine the amount of gene expression in bulbo-urethral glands. An external standard curve was generated and developed with TaqMan® technology. Based on two in vivo experiments, the decision limit CCα, sensitivity and specificity of this screening method were established. Trial 1 consisted of 32 Friesian veal calves divided into two groups: group A (n?=?12), consisting of animals treated with four doses of 17β-oestradiol (5?mg?week^?1 per animal); and group B (n?=?20), consisting of control animals. Trial 2 was performed on 26 Charolaise beef cattle that either received five doses of 17β-oestradiol (group C; 20?mg?week^?1 per animal; n?=?6) or remained untreated (group D; n?=?20). Further, progesterone receptor gene expression was evaluated in beef and veal calves for human consumption. A specific CCα on 20 Piedmontese control beef cattle was calculated to include these animals in a field investigation. Five out of 190 beef cattle and 26 out of 177 calves tested expressed the progesterone receptor gene above their respective CCα and they were classified as being suspected of 17β-oestradiol treatment. Additionally, 58% of veal calves that tested suspect via qPCR exhibited histological lesions of the bulbo-urethral gland tissue, which are typical of oestrogen administration and are consistent with hyperplasia and metaplasia of the glandular epithelium.     

Investigating the within-herd prevalence and risk factors for ketosis in dairy cattle in Ontario as diagnosed by the test-day concentration of β-hydroxybutyrate in milk

An observational study of 790 to over 3,000 herds was conducted to estimate the within-herd prevalence and cow-level risk factors for ketosis in dairy cattle in herds that participate in a Dairy Herd Improvement Association (DHIA) program. Ketosis or hyperketolactia (KET) was diagnosed as milk β-hydroxybutyrate ≥0.15 mmol/L at first DHIA test when tested within the first 30 d in milk. Seven hundred ninety-five herds providing at least 61 first milk tests from June 2014 to December 2015 were used to estimate the provincial within-herd prevalence of KET. All herds on DHIA in Ontario (n = 3,042) were used to construct cow-level multilevel logistic regression models to investigate the association of DHIA collected variables with the odds of KET at first DHIA milk test. Primiparous and multiparous animals were modeled independently. The cow-level KET prevalence in Ontario was 21%, with an average within-herd prevalence of 21% (standard deviation = 10.6) for dairy herds enrolled in a DHIA program. The prevalence of KET had a distinct seasonality with the lowest prevalence occurring from July to November. Automatic milking systems (AMS) were associated with increased within-herd prevalence, as well as increased odds of KET in multiparous animals at first test (odds ratio: 1.45; 95% confidence interval: 1.30 to 1.63). Jersey cattle had over 1.46 times higher odds of KET than Holstein cattle. Milk fat yield ≥1.12 kg/d at the last test of the previous lactation was associated with decreased odds of KET in the current lactation (odds ratio: 0.56; 95% confidence interval: 0.53 to 0.59). Increased days dry and longer calving intervals, for multiparous animals, and older age at first calving for primiparous animals increased the odds of KET at first test. This study confirms previous findings that increased days dry, longer calving intervals, and increased age at first calving are associated with increased odds of KET and is the first report of increased KET in herds with AMS and in relation to milk fat yield at the final test of the previous lactation. Feeding management on AMS herds likely contributes to the increased prevalence of KET and further work is required to investigate modifications to current management to minimize risk. Milk fat yield during the previous lactation may be representative of energy partitioning.     

Recovery of heat-stressed E. coli O157:H7 from ground beef and survival of E. coli O157:H7 in refrigerated and frozen ground beef and in fermented sausage kept at 7°C and 22°C

A study was undertaken to obtain information on survival of Escherichia coli O157:H7 in ground beef subjected to heat treatment, refrigeration and freezing and on survival of E. coli O157:H7 in fermented sausage kept at 7°C and 22°C. For the challenge test, a mixture of E. coli O157:H7 strains (EH 321, EH 385, EH 302) was used and enumeration was performed on an isolation medium suitable for recovery of stressed organisms: modified Levine's eosin methylene blue agar (mEMB). Heat resistance of E. coli O157:H7 decreased after pre-incubation at a reduced temperature.Escherichia coli O157:H7 was more susceptible to heat inactivation after storage at 7°C and die-off was even more enhanced if cultures were frozen prior to heat inactivation. The enhanced reduction of the pathogen at 56°C after prior storage under refrigeration was confirmed in a test with inoculated ground beef.Escherichia coli O157:H7 was able to survive in ground beef at 7°C for 11 days and at −18°C for 35 days showing maximal one log reduction during the storage period. Thus, ground beef contaminated with E. coli O157:H7 will remain a hazard even if the ground beef is held at low or freezing temperatures. At both 7°C and 22°C, a gradual reduction of E. coli O157:H7 was noticed in fermented sausage over the 35 days storage period resulting in a 2 log decrease of the high inoculum (10⁶cfu 25 g⁻¹). For the low inoculum (10³cfu 25 g⁻¹) a 2·5 log reduction was obtained in 7 and 28 days storage at respectively 22 and 7°C. Application of good hygienic practices and implementation of HACCP in the beef industry are important tools in the control of E. coli O157:H7.     

Confirmation of the progesterone receptor as an efficient marker of treatment with 17β-estradiol in veal calves

Under current European Union legislation the use of anabolic steroids in food-producing livestock is banned because of their long-term adverse effects on human health. We examined the expression profile of the immunohistochemical marker progesterone receptor in veal calves’ sex accessory glands following experimental administration of anabolic compounds. The aim was to confirm the accuracy of the immunohistochemical approach in the detection of the over-expression of the progesterone receptor induced by the administration of sexual steroids at low levels (17β-estradiol and nandrolone alone or in combination). A total of 217 male veal calves were randomly divided into four groups: group A (104 calves) treated with 17β-estradiol (5 mg/head; 4 weekly injections); group B (20 calves) treated with nandrolone (50 mg/head; 4 weekly injections); group C (20 calves) treated with the association of the two steroids (5 mg estradiol + 50 mg nandrolone; 4 weekly injections); and group K (73 calves) kept as a control. All the sexual accessory glands were collected at the slaughterhouse (15 days after the last administration) and subjected to immunohistochemical staining with anti-progesterone receptor antibody. All the calves treated with 17β-estradiol alone or in association with nandrolone (groups A and C) showed strong positivity, while nandrolone-treated calves and controls (groups B and K) gave negative results to the immunohistochemical investigation. The statistical analysis showed that the progesterone receptor is a significant predictor of 17β-estradiol treatment alone or in association with nandrolone (p < 0.001): the immunohistochemical study resulted in 100% sensitivity (CI = 95%: 97.1–100%) and specificity (CI = 95%: 95.1–100%) for prostate and 99% sensitivity (CI = 95%: 95.6–100%) and 100% specificity (CI = 95%: 95.1–100%) for bulbo-urethral glands. The data confirm that this innovative biological approach offers a reliable tool to enhance the efficacy of the histological test to detect illegal treatments with estrogens alone or in association with androgens.     

Can routinely recorded reproductive events be used as indicators of disease emergence in dairy cattle? An evaluation of 5 indicators during the emergence of bluetongue virus in France in 2007 and 2008

In response to increasing risks of emerging infectious diseases, syndromic surveillance can be a suitable approach to detect outbreaks of such diseases across a large territory in an early phase. To implement a syndromic surveillance system, the primary challenge is to find appropriate health-related data. The objective of this study was to evaluate whether routinely collected dates of reproductive events in dairy cattle could be used to build indicators of health anomalies for syndromic surveillance. The evaluation was performed on data collected in France between 2003 and 2009. First, a set of 5 indicators was proposed to assess several types of reproductive disorders. For each indicator, the demographic coverage over the total number of cattle at risk was analyzed in time and space. Second, the ability to detect an emerging disease in an early phase was retrospectively evaluated during epidemics of bluetongue serotypes 1 and 8 (BTV-1, BTV-8) in France in 2007 and 2008. Reproductive indicators were analyzed weekly during these epidemics for each indicator in each infected French district (16 in 2007 and 50 in 2008 out of 94 districts). The indicators were able to detect the BTV epidemics despite their low demographic coverage on a weekly basis relatively to total number of cattle (median = 1.21%; range = 0–11.7%). Four indicators related to abortions, late embryonic death, and short gestations were abnormally elevated during both BTV epidemics. Median times to abnormal elevations in these indicators were 20 to 71 d after the first notification of clinical signs of BTV by veterinarians. These results demonstrate that reproduction data can be used as indicators of disease emergences, whereas in the specific case of these BTV epidemics, detection via these indicators was later than clinical detection by veterinarians. The emergence of bluetongue in 2007 in France was associated with gestations that were a few days shorter than expected. A short gestation indicator underwent high elevations relative to prior random fluctuations and was the earliest (out of the 4 indicators) to show abnormal elevations, making it possible to detect this emergence.     

Effects of vitamin A supplementation on growth performance, carcass characteristics and meat quality in Limosin×Luxi crossbreed steers fed a wheat straw-based diet

Three experiments were conducted to examine the effects of dietary vitamin A supplementation on performance and carcass parameters in Limosin × Luxi crossbreed finishing steers fed a wheat straw-based diet. Sixteen 12-month old (301 ± 22 kg) steers, 16 12-month old (309 ± 15 kg) steers and 16 24-month old (411 ± 20 kg) steers were used in experiment 1 for 6 months feeding period, in experiment 2 for three months feeding period and in experiment 3 for three months feeding period, respectively. Sixteen steers of each experiment were randomly divided into the four groups of four animals. Treatments consisted of four vitamin A supplementation levels (0, 1100, 2200 and 4400IU/kg DM). The growth rate was affected by dietary vitamin A level in experiment 1 and 2, revealing that the suitable amount of vitamin A supplementation increased the growth rate; excessive vitamin A in the ration decreased the growth rate of 12-month-old finishing steers. The marbling deposition decreased with the increment of vitamin A supplementation level, but possibly associated with vitamin A supplementing duration. Furthermore, the suitable dietary vitamin A level probably decreased lipid and pigment oxidation, and increased the tenderness of beef meat. Vitamin A supplementation had no significant effect on chemical composition of gluteus medius muscle and longissimus dorsi muscle.