代谢分析用于丙酮酸发酵的研究进展

丙酮酸是重要的有机合成中间体,广泛应用于医药、化妆品、化学生产等工业中。但目前以发酵法生产丙酮酸的产量不高。近年来,有很多研究者通过代谢控制手段来提高乳酸、缬氨酸、丙酮酸等有机酸的产量。综述了代谢分析在丙酮酸发酵工艺优化中的研究应用进展。     

光滑球拟酵母发酵生产丙酮酸

丙酮酸是一种重要的有机酸 ,广泛应用于制药、日化、农用化学品和食品等工业中。相对于化工法生产的丙酮酸而言 ,发酵法生产的丙酮酸具有低成本、高质量等优势。考虑到球拟酵母属的多重维生素营养缺陷型菌株是目前发酵法生产中最常用和最具竞争力的生产菌株 ,因此重点介绍了发酵法生产丙酮酸在菌种、发酵条件优化等方面的研究进展 ,并给出了发酵法将来可能的发展方向。     

丙酮酸甲酯的合成

本文介绍了以丙酮酸和无水甲醇为原料,加入合适的催化剂合成丙酮酸甲酯的过程。通过对反应物配比、带水剂、反应时间等影响因素进行对比试验,确定以四氯化碳为带水剂,最佳反应配比为:丙酮酸∶无水甲醇∶四氯化碳=1∶2.5∶0.28,产物收率可达73.9%。该工艺过程简单,易操作,适合工业化大生产。     

丙酮酸的合成研究进展

丙酮酸作为一种重要的有机化工产品，日益广泛地应用于医药、农药、日化和食品等行业。本文从化学合成法和生物技术法两个方面，详细介绍了丙酮酸合成现状及其研究进展，并对丙酮酸的发展前景进行了展望。通过对上述两种方法优缺点的比较，指出生物技术法具有原料成本低、产物纯度高、反应条件温和等优点，具有良好的发展前景。     

我国丙酮酸及系列产品合成工艺10 a进展

综述了丙酮酸及系列产品近10 a来合成工艺的发展状况。介绍了目前国内生产丙酮酸的各种合成工艺方法,包括酒石酸法、乳酸氧化法、直接发酵法和酶催化法,阐述了它们的合成反应原理、工艺参数、产率等,评价了这些方法的利弊与发展前景。     

丙酮酸荧光毛细生物传感器的研究

基于荧光毛细法(FCA)和固定化酶技术,开发了一种新的丙酮酸荧光毛细生物传感器(PF-CB)。在激发波长350nm、发射波长460nm条件下,成功地对丙酮酸进行了测定;其线性范围为0.2-0.8mmol/L,r=0.9963。PFCB法操作简便,样品用量仅为18μL,测定成本低;可实现丙酮酸的微量化、快速化测定;可制成荧光毛细丙酮酸测试盒,用于含丙酮酸样品的测定。     

溴代丙酮酸的合成新工艺

以丙酮酸和二溴海因为原料制备了溴代丙酮酸。优化工艺条件为:反应温度为50℃,催化剂为对甲苯磺酸,溶剂为甲醇,收率为40%。该新工艺具有操作友好、后处理简单等优势。     

抗癌药物比卡鲁胺

介绍过期专利抗癌药物比卡鲁胺的基本性能和作用。并概述了分别以溴丙酮、2-甲基丙烯酰氯、2-甲基丙烯酸甲酯、2,3-二羟基-2-甲基丙酸以及丙酮酸或丙酮酸酯为起始原料合成比卡鲁胺的方法。其中以丙酮酸或丙酮酸酯为原料的合成方法为最佳。     

丙酮酸镁合成工艺的研究

研究了丙酮酸与碳酸镁反应合成丙酮酸镁的工艺过程.通过考察不同的原料配比、反应温度、反应时间以及终点控制,确定了最佳的合成条件:n(丙酮酸):n(碳酸镁)=2.1:1,在温度为65℃下反应60min,反应终点pH在4左右;在此条件下,丙酮酸镁的收率可达94.4%.     

酯化法合成丙酮酸乙酯的研究

论述了以丙酮酸、乙醇为原料,离子交换树脂作催化剂,催化合成丙酮酸乙酯的新工艺。考察了催化剂种类、用量、原料配比、反应时间以及催化剂使用次数等因素对酯化率的影响,确定了催化剂种类和最佳工艺条件。结果表明,732强酸性离子交换树脂具有良好的催化活性和选择性,可多次重复使用,酯化率可达85.7%,产品质量符合国家标准。     

水溶性丙酮酸锌的合成工艺研究

采用丙酮酸与乙酸锌反应合成水溶性丙酮酸锌,确定了适宜的工艺条件。合成水溶性丙酮酸锌最佳的反应条件是:以乙酸乙酯和丙酸为溶剂,其体积比为1∶0.7,丙酮酸与乙酸锌摩尔配比为1∶0.55,在20~25℃温度下反应。得到的产品的结构经红外和核磁的确证,其结构式为(CH3COCOO)2Zn.1/2H2O,得到的产品在水中具有较好的溶解性。     

双氧水催化氧化乳酸合成丙酮酸

本文研究了以乳酸为原料，用双氧水催化氧化合成丙酮酸，并探讨了催化剂，温度，原料配比，反应时间等因素对合成反应的影响，确定了最佳合成条件。     

Selective electroreduction of pyruvic acid on lead electrode in acid medium

This work reports the electrochemical reduction of pyruvic acid, which contains a carbonyl group more reducible than its carboxylic entity. The electrode potential and the concentration of the starting substrate seemed to be the factors that control the electrochemical production of the competitive reaction products. In fact, lactic acid was mainly obtained on a lead electrode in sulfuric acid for low concentrations of pyruvic acid while 2,3-dimethyl tartaric acid was selectively formed by electrohydrodimerization of a high amount of pyruvic acid. During the cathodic reduction of pyruvic acid in sulfuric acid solution, the effects of electrode potential and pyruvic acid concentration on the selectivity towards the formation of lactic acid were systematically studied.     

Electrosynthesis of 2,3-dimethyltartaric acid from pyruvic acid in acid medium

This study reports the electrohydrodimerization of pyruvic acid to 2,3-dimethyltartaric acid in sulphuric acid medium (0.5 M H₂SO₄) on a lead cathode. The main products detected were lactic acid and 2,3-dimethyltartaric acid. The selectivity towards the formation of 2,3-dimethyltartaric acid was studied vs. pyruvic acid concentration in sulphuric acid solution, at −1.1 V vs. MSE. The best selectivity of 2,3-dimethyltartaric acid reached 69% for an initial concentration of 1.7 M pyruvic acid. The yield of pyruvic acid was 84%.     

弱碱性树脂吸附分离丙酮酸的特性研究

Uptakes of pyruvic acid for two types of commercially available weakly basic polymer sorbents, D301G and D301R, have been measured over a wide pH range and at various salinities of MgSO4. The results show that the overloading adsorption of pyruvic acid occurs on both weakly basic polymer sorbents, and the overloading models can predict the experimental data of uptake very well. The overloading value for D301G is larger than that for D301R. The adsorption isotherm of pyruvic acid for both polymeric sorbents is greatly affected by the solution pH and MgSO4 concentration in the aqueous phase, and a high recovery efficiency of pyruvic acid from aqueous solution can be obtained at the solution pH around 2.     

Recovery of pyruvic acid with weakly basic polymeric sorbents

BACKGROUND: Carboxylic acids are among the most important substances that can be manufactured from biomass. However, the recovery of carboxylic acids from fermentation broths presents a challenging separation problem. To avoid the production of waste salts and net consumption of chemicals in the calcium carboxylate salt process, the use of reversible chemical complexation with polymeric sorbents and extractants is attractive for carboxylic acid recovery. Pyruvic acid is widely used in the manufacture of medicines, pesticides and foodstuffs and can be produced by fermentation. Since the acidity of pyruvic acid (pK_a = 2.49) is stronger than that of normal carboxylic acids, and as few reports on the recovery of pyruvic acid are available, the sorption of pyruvic acid from aqueous solution on two types of weakly basic polymeric sorbent, tertiary amine D301R and primary amine D392, was investigated over a wide pH range and at various salt (MgSO₄) concentrations. RESULTS: Overloading adsorption of pyruvic acid on both weakly basic polymeric sorbents occurred, with the overloading of D392 being greater than that of D301R. The adsorption of pyruvic acid on both sorbents was greatly affected by the solution pH and the salt concentration in the aqueous phase. An overloading model was able to predict the experimental uptake data very well. CONCLUSION: Solution pH is one of the most important operating conditions, and both polymeric sorbents D392 and D301R can be used to recover pyruvic acid from dilute aqueous solution with high efficiency at a solution pH around 2. The uptake by D392 is greater than that by D301R owing to steric hindrance of the tertiary amine. Copyright © 2008 Society of Chemical Industry     

丙酮酸高产菌诱变选育及发酵工艺优化的初步研究

以光滑球拟酵母(Torulopsis glabrata)HB20为出发菌,经过UV和DES复合诱变选育出一株丙酮酸高产菌HB304.鉴定其为维生素营养缺陷型,摇瓶发酵产酸量比原菌增加了31.2%;优化培养基后其摇瓶发酵产酸量比原菌增加了41.9%.     

丙酮酸化壳聚糖纳米粒子制备研究

壳聚糖在印染、环保、食品、日化、医药等领域的应用十分广泛。但壳聚糖也有局限性,为了进一步拓展壳聚糖的应用范围和开发出一些性能优良的新化合物,所以要对壳聚糖进行化学改性。文章利用壳聚糖C2位上活泼氨基与丙酮酸进行席夫碱反应,制取丙酮酸化壳聚糖。