MiR-195对BEL-7402/5-FU细胞5-氟尿嘧啶耐药性的影响

目的探讨miR-195对BEL-7402/5-FU细胞5-氟尿嘧啶药物敏感性的影响。方法采用qRTPCR检测BEL-7402细胞与BEL-7402/5-FU细胞中miR-195的表达水平。将miR-195质粒载体瞬时转染至BEL-7402/5-FU细胞;MTT检测细胞药物敏感性的改变。结果相比于BEL-7402细胞,miR-195在BEL-7402/5-FU细胞中表达水平下调;miR-195转染组的细胞增殖抑制率较miRNA阴性对照组及未转染组明显增高。结论MiR-195能够增加BEL-7402/5-FU细胞对5-氟尿嘧啶的药物敏感性,抑制细胞增殖。     

酯类聚羧酸系高性能减水剂的研究

本文采用分子设计的方法,首先使用甲氧基聚乙二醇(MPEG)和甲基丙烯酸进行酯化反应合成大分子单体,然后与其他小分子单体进行自由基聚合,合成不同结构的酯类聚羧酸系高性能减水剂。本文研制的酯类聚羧酸系高性能减水剂(PC1)在保持砂浆流动度基本不变的条件下,减水率可达到36%;掺加PC1的混凝土坍落度损失小,混凝土抗压强度较空白样明显提高,3天抗压强度可提高200%,28天强度可提高100%。     

萘系高效减水剂合成工艺中水解反应过程的研究

萘系高效减水剂合成工艺中水解反应过程的控制对最终产品的减水性能影响很大。对萘系高效减水剂合成工艺中的水解过程进行了系统的研究,结果表明,最佳水解终了酸度为28%～30%;由于水解终了酸度检测的滞后性,所以使用水解用水量和酸度双因素比单用水解终了酸度控制水解过程更准确;在满足酸度要求的情况下,尽可能减少水解反应用水量可有效提高萘系高效减水剂的减水性能。     

Mechanism of natural organic matter removal by polyaluminum chloride: effect of coagulant particle size and hydrolysis kinetics

The mechanism of natural organic matter (NOM) removal by AlCl(3) and polyaluminum chloride (PACl) was investigated through bench-scale tests. The fraction distributions of NOM and residual Al after coagulation in solution, colloid and sediment were analyzed as changes of coagulant dosage and pH. The influence of NOM, coagulant dose and pH on coagulation kinetics of AlCl(3) was investigated using photometric dispersion analyzer compared with PACl. Monomeric Al species (Al(a)) shows high ability to satisfy some unsaturated coordinate bonds of NOM to facilitate particle and NOM removal, while most of the flocs formed by Al(a) are small and difficult to settle. Medium polymerized Al species (Al(b)) can destabilize particle and NOM efficiently, while some flocs formed by Al(b) are not large and not easy to precipitate as compared to those formed by colloidal or solid Al species (Al(c)). Thus, Al(c) could adsorb and remove NOM efficiently. The removal of contaminant by species of Al(a), Al(b) and Al(c) follows mechanisms of complexation, neutralization and adsorption, respectively. Unlike preformed Al(b) in PACl, in-situ-formed Al(b) can remove NOM and particle more efficiently via the mechanism of further hydrolysis and transfer into Al(c) during coagulation. While the presence of NOM would reduce Al(b) formed in-situ due to the complexation of NOM and Al(a).     

剩余污泥碱解上清液回用于生活污水脱氮研究

考察了剩余污泥碱解上清液作为反硝化碳源用于生活污水脱氮的可行性,经试验分析得出pH=10,SRT=9 d时产生的上清液和乙酸的反硝化速率相当,大大提高了生活污水反硝化性能,且不影响微生物活性。     

超缓凝高效减水剂GCL1—5的性能研究

为了适应大型混凝土工程和高温施工的需要,本文在改性木素磺酸钙缓凝高效减水剂GCL1的基础上进一步进行化学改性,研制出超缓凝高效减水剂GCL1 5,并对其进行性能研究。结果表明:掺加0 4%GCL1 5时,水泥净浆在20℃下初凝时间为26h25min,比未掺加减水剂时延缓了23h;40℃下初凝时间为4h50min,比未掺加减水剂时延缓了3h,缓凝效果显著。20℃时2h内的相对流动度损失为30%,40℃时为42 8%,能明显减小流动度损失。掺加GCL1 5的硬化水泥7d、28d、90d的抗压强度比分别为114%、125%、136%。扫描电镜研究的结果表明,GCL1 5使硬化水泥的后期强度明显提高的原因是GCL1 5能使水化产物致密、水泥石毛细孔径变小。     

磷系阻燃聚乙烯热降解动力学研究

采用热重分析方法研究纯聚乙烯、阻燃聚乙烯在空气气氛中的热降解行为,并采用Kissinger法和Flynn-Wall-Ozawa(FWO)法计算其热降解动力学参数。计算结果表明,阻燃聚乙烯的活化能高于纯聚乙烯,阻燃剂的加入提高了聚乙烯的热稳定性。     

氮气气氛下烟叶的热解动力学研究

采用热分析法,在氮气气氛下对云南产5种烟叶样品的热解过程进行研究,发现各烟叶样品的热解过程相似,大致经历了3个阶段,其中失重主要发生在快速热解阶段(120～350℃),失重率约占总失重率的75%。利用Coats-Redfern积分法建立了5种样品在主要失重阶段的"双组分两阶段"一级反应动力学模型,并计算了热解动力学参数,其线性相关性在0.97以上。     

The inactivation kinetics of H-1 parvovirus by chlorine

A newly isolated enteric virus has recently been found to be associated with large outbreaks of waterborne gastroenteritis. Most commonly referred to as the Norwalk agent, this virus appears to be morphologically and biophysically similar to the parvoviruses. In this study the parvovirus H-1, a putative human virus containing single-stranded DNA, was used as a model virus for chlorine inactivation experiments. The objective of this research was to investigate the kinetics of inactivation of this virus by low levels of free chlorine (0.05–0.20 mgl⁻¹) at pH 7 and at 5, 10, 20 and 30°C.Inactivation occurred in the usual dose-response relationship, that is, increasing the chlorine dose caused an increase in the rate of inactivation. The energy required for the inactivation reaction using 0.05 mgl⁻¹ free chlorine from 5 to 30°C was graphically determined to be 2.4 kcal mol⁻¹. The change in entropy was calculated to be -52.34 entropy units. For disinfection purposes, the time required for 99% inactivation of H-1 parvovirus at pH 7.20°C and a chlorine dose of 0.2 mgl⁻¹ free chlorine was 3.2 min. The parvovirus H-1 appeared to be less resistant to free chlorine than poliovirus type 1 (LSc).     

A waste characterisation procedure for ADM1 implementation based on degradation kinetics

In this study, a procedure accounting for degradation kinetics was developed to split the total COD of a substrate into each input state variable required for Anaerobic Digestion Model n°1. The procedure is based on the combination of batch experimental degradation tests (“anaerobic respirometry”) and numerical interpretation of the results obtained (optimisation of the ADM1 input state variable set). The effects of the main operating parameters, such as the substrate to inoculum ratio in batch experiments and the origin of the inoculum, were investigated. Combined with biochemical fractionation of the total COD of substrates, this method enabled determination of an ADM1-consistent input state variable set for each substrate with affordable identifiability. The substrate to inoculum ratio in the batch experiments and the origin of the inoculum influenced input state variables. However, based on results modelled for a CSTR fed with the substrate concerned, these effects were not significant. Indeed, if the optimal ranges of these operational parameters are respected, uncertainty in COD fractionation is mainly limited to temporal variability of the properties of the substrates. As the method is based on kinetics and is easy to implement for a wide range of substrates, it is a very promising way to numerically predict the effect of design parameters on the efficiency of an anaerobic CSTR. This method thus promotes the use of modelling for the design and optimisation of anaerobic processes.     

环氧—丙烯酸酯型路面微裂缝修补材料研究

对预聚时间与改性环氧—丙烯酸酯型微裂缝修补材料固化时粘度变化规律和初始固化时间的关系进行了分析,指出材料固化情况对预聚时间的变化很敏感,预聚时间应控制在10 min~20 min。