The effect of polymer addition on granulation in an anaerobic baffled reactor (ABR). Part I: process performance

The stability and performance of an anaerobic baffled reactor (ABR) treating an ice-cream wastewater at several organic loading rates have been investigated. Specifically, it was determined whether an ABR would promote phase separation and if a polymer additive was capable of enhancing granule formation in an ABR. In order to achieve these goals, two ABRs, having identical dimensions and configurations, were used to study the above objectives using a synthetic ice-cream wastewater. The ABR proved to be an efficient reactor configuration for the treatment of a high-strength synthetic ice-cream wastewater. An organic loading rate of around 15 kg CODm(-3) d(-1) was treated with a 99% COD removal efficiency. From the jar test and inhibition assay, it was concluded that Kymene SLX-2 was the most effective and least inhibitory polymer tested. The methane yield was higher in the polymer-amended reactor compared to the control reactor. In addition, polymer addition resulted in a considerably higher degree of biomass retention and lower solids washout from the ABR. Consequently, it demonstrated that there was a considerable potential for sludge conditioning in ABRs by facilitating better biomass retention within the reactor which in turn led to better process performance. Granulation was achieved in both ABRs within 3 months. However, the granules from the polymer-amended reactor appeared earlier and were generally larger and more compact, although this was not quantified in detail during the present study. The main advantage of using an ABR comes from its compartmentalised structure. The first compartment of an ABR may act as a buffer zone to all toxic and inhibitory material in the feed thus allowing the later compartments to be loaded with a relatively harmless, balanced and mostly acidified influent. In this respect, the latter compartments would be more likely to support active populations of the relatively sensitive methanogenic bacteria and partly explains why the best granules and the highest methane yield were obtained in Compartment 2. It is unlikely that a complete separation of phases (acidogenic and methanogenic) occurred within the ABRs since methane production was observed in all compartments, although this was low (approximately 40% of all gas composition) in Compartment 1, becoming higher (approximately 70%) in the following compartments.     

The role of formate in the anaerobic baffled reactor

The anaerobic baffled reactor (ABR) contains a mixed anaerobic culture segregated into compartments. During pseudo-steady state runs, formate was detected in the first two or three compartments, thereafter dropping off sharply. Under conditions of shock loading, formate was detected in the reactor effluent, up to peak concentrations of 2500 mg/l. There are indications that formate may play an important role as an intermediate in the anaerobic digestion process, and that its production may contribute significantly to reactor stability.     

The distribution of bacterial activity in an anaerobic fluidized bed reactor

The relative phosphatase activity and the methane production rate of the biomass at different heights in an anaerobic fluidized bed reactor have been determined, together with volatile acid concentration profiles at three organic loadings. There was some evidence of partial phase separation within the reactor with the highest methane production rates being above the peak in phosphatase activity. Attached biomass had the highest relative activities and was considered to have a more important role in anaerobic stabilization than the unattached portion.     

The adsorption of heavy metals in an acidogenic thermophilic anaerobic reactor

A two-phase, thermophilic anaerobic reactor was operated with a starch-based feed. Copper, zinc, nickel and lead (3 mM) were individually added to the feed each for a period of 30 h. The results were analysed to determine the extent of metal binding in the acidogenic, first-stage reactor and the degree of protection that this afforded to the traditionally more sensitive methanogenic phase. The results showed that zinc and nickel were not bound particularly well, that lead was bound very strongly and that copper had binding characteristics that were between these two extremes. When these findings were compared with an earlier study with a mesophilic sludge, zinc was shown to have a very different behaviour. A possible reason for this is given. An examination of the gas production by the methanogenic stage, in relation to the amount of metal reaching this stage, suggested that the phase separation did not offer any real protection from the toxic effects of heavy metals.     

Investigation of bacterial community in activated sludge with an anaerobic side-stream reactor (ASSR) to decrease the generation of excess sludge

The goal of this study was to investigate the bacterial community in activated sludge with an anaerobic side-stream reactor (ASSR), a process permitting significant decrease in sludge production during wastewater treatment. The study operated five activated sludge systems with different sludge treatment schemes serving as various controls for the activated sludge with ASSR. Bacterial communities were analyzed by denaturing gradient gel electrophoresis (DGGE), sequencing and construction of phylogenetic relationships of the identified bacteria. The DGGE data showed that activated sludge incorporating ASSR contained higher diversity of bacteria, resulting from long solids retention time and recirculation of sludge under aerobic and anaerobic conditions. The similarity of DGGE profiles between ASSR and separate anaerobic digester (control) was high indicating that ASSR is primarily related to conventional anaerobic digesters. Nevertheless, there was also unique bacteria community appearing in ASSR. Interestingly, sludge in the main system and in ASSR showed considerably different bacterial composition indicating that ASSR allowed enriching its own bacterial community different than that from the aeration basin, although two reactors were connected via sludge recirculation. In activated sludge with ASSR, sequences represented by predominant DGGE bands were affiliated with Proteobacteria. The remaining groups were composed of Spirochaetes, Clostridiales, Chloroflexi, and Actinobacteria. Their putative role in the activated sludge with ASSR is also discussed in this study.     

Saline sewage treatment using a submerged anaerobic membrane reactor (SAMBR): Effects of activated carbon addition and biogas-sparging time

This study investigated the performance of a submerged anaerobic membrane reactor (SAMBR) treating saline sewage under fluctuating concentrations of salinity (0-35 g NaCl/L), at 8 and 20 h HRT, with fluxes ranging from 5-8 litres per square metre per hour (LMH). The SAMBRs attained a 99% removal of Dissolved Organic Carbon (DOC) with 35 g NaCl/L, while removal inside the reactor was significantly lower (40-60% DOC). Even with a sudden drop in salinity overall removal recovered quickly, while the recovery inside the reactor took place at a slower rate. This highlights the positive effect of the membrane in preventing the presence of high molecular weight organics in the effluent while also retaining biomass inside the reactor so that they can rapidly acclimatize to salinity. The reduction of continuous biogas sparging to intervals of 10 min ON and 5 min OFF resulted in a slight increase in transmembrane pressure (TMP) by 0.025 bar, but also resulted in an increase in effluent DOC removal and inside the SAMBR by 10% and 20%, respectively. The addition of powdered activated carbon (PAC) resulted in a decrease in the TMP by 0.070 bar, and an increase in DOC removal in the reactor and effluent by 30% and 5%, respectively. The PAC dramatically decreased the high molecular weight organics in the reactor over a period of 72 h. SEM pictures of the membrane and biomass before and after addition of PAC revealed a remarkable reduction of flocks on the membrane surface, and a reduction inside the reactor of soluble microbial products (SMPs). Finally, Energy Dispersive X-ray (EDX) analysis of the membranes pores and biofilm highlighted the absence of organic matter in the inner pores of the membrane.     

The effect of increased nickel ion concentrations on microbial populations in the anaerobic digestion of sewage sludge

Dissolved nickel at concentrations above 1 mg l⁻¹ was inimical to the methanogenic process either directly or indirectly via the toxic metabolic end-products of nickel tolerant degradative organisms. Many hydrolytic species of bacteria in the digester were inhibited at levels of dissolved nickel above 12 mg l⁻¹. Increased nickel concentrations inhibited carbohydrate fermentation, as judged by starch hydrolysis, while protein hydrolysing (and amino acid fermenting) populations of bacteria were selected for. Dosing with nickel caused a build up of higher molecular weight fatty acids in the digester, several of which are derived exclusively from amino acid fermentation, highlighting the altered hydrolytic emphasis of the more nickel tolerant bacterial population. Nickel, as a toxic heavy metal, exerts a primary toxic effect in the area of methanogenesis but is less toxic to the hydrolytic aspect of the overall digestion process.     

Acidogenesis of gelatin-rich wastewater in an upflow anaerobic reactor: influence of pH and temperature

The influence of temperature and pH on the acidification of a synthetic gelatin based wastewater was investigated using an upflow anaerobic reactor. Gelatin degradation efficiency and rate, degree of acidification, and formation rate of volatile fatty acids and alcohols all slightly increased with temperature. Temperature affected the acidogenesis of gelatin according to the Arrhenius equation with an activation energy of 1.83 kcal/mol. Compared with temperature, pH had a more significant effect on the acidogenesis. Gelatin degradation efficiency substantially increased with pH, from 60.0% at pH 4.0 to 97.5% at pH 7.0. The degree of acidification increased from 32.0% at pH 4.0 to 71.6% at pH 6.5, but dropped to 66.8% when pH increased to 7.0. The optimum pH for the overall acidogenic activity was found to be 6.0, close to 5.9, the optimum pH calculated using a semi-empirical model. Operation at pH of 4.0-5.0 favored the production of propionate, hydrogen, whereas the operation at pH 6.0-7.0 encouraged the production of acetate, butyrate, and i-butyrate. The region between pH 5.0 and 6.0 was the transition zone.     

Integrated removal of nitrate and carbon in an upflow anaerobic sludge blanket (UASB) reactor: Operating performance

Denitrification and methanogenesis of a synthetic wastewater containing volatile fatty acids and nitrate were obtained in a single-stage process using an upflow anaerobic sludge blanket (UASB) reactor. The reactor was initially inoculated with methanogenic granular sludge and was gradually adapted to nitrate by increasing the nitrate concentration in the influent. Excess carbon not utilized for denitrification was converted to methane. During steady-state at a loading of 336 mg NO₃-N/l/d (24 mmol NO₃/l/d) and 6600 mg COD/l/d more than 99% removal of both nitrate and carbon was achieved. Batch experiments with biomass from the reactor showed that approximately 90% of the added nitrate was recovered as nitrogen gas indicating that true dentrification occurred. This was further verified from mass balances over the reactor. The granules changed appearance during the first 5 months of operation being fluffy and buoyant, probably reflecting changes in the microbial composition induced by the presence of nitrate. However, during the next two months more dense granules with good settling abilities gradually established in the system making this kind of combined process feasible in a UASB reactor. Characterization of the produced granules showed that while the mean diameter and density was comparable to granules from purely methanogenic systems, although the strength was lower.     

Performance of an up-flow anaerobic stage reactor (UASR) in the treatment of pharmaceutical wastewater containing macrolide antibiotics

The performance of an up-flow anaerobic stage reactor (UASR) treating pharmaceutical wastewater containing macrolide antibiotics was investigated. Specifically, it was determined whether a UASR could be used as pre-treatment system at an existing pharmaceutical production plant to reduce the antibiotics in the trade effluent. Accordingly, a UASR was developed with an active reactor volume of 11 L being divided into four 2.75 L stages. Each stage of the reactor was an up-flow sludge blanket reactor and had a 3-phase separator baffle to retain biomass. The reactor was fed with real pharmaceutical wastewater containing Tylosin and Avilamycin antibiotics and operated with step-wise increases in the reactor organic loading rate (OLR) from 0.43 to 3.73 kg chemical oxygen demand (COD) m(-3)d(-1), and then reduced to 1.86, over 279 days. The process performance of the reactor was characterised in terms of its COD removal, Tylosin reduction, pH, VFA production, methane yield and sludge washout. At a total hydraulic retention time (HRT) of 4 d and OLR of 1.86 kg COD m(-3)d(-1), COD reduction was 70-75%, suggesting the biomass had acclimated to the antibiotics. Furthermore, an average of 95% Tylosin reduction was achieved in the UASR, indicating that this antibiotic could be degraded efficiently in the anaerobic reactor system. In addition, the influence of elevated Tylosin concentrations on the UASR process performance was studied using additions of Tylosin phosphate concentrate. Results showed similar efficiency for COD removal when Tylosin was present at concentrations ranging from 0 to 400 mgL(-1) (mean removal over this range was 93%), however, at Tylosin concentrations of 600 and 800 mgL(-1) there was a slight decline in treatment efficiency at 85% and 75% removal, respectively.     

The effect of high speed mixing and polymer dosing rates on the geometric and rheological characteristics of conditioned anaerobic digested sludge (ADS)

Wastewater sludges are non-Newtonian fluids because their shear rates change with shear stress and no linear relationship is observed between their shear stress and shear rate. Therefore, it is necessary to condition sludges with polyelectrolytes prior to dewatering to increase in the dewaterability. Since 1978, researchers have observed that the yield strength of conditioned sludge increased with the addition of polymer up to the optimum dose. Then sludge rheogram was used as a control parameter to optimize the addition of polymers, and the peak height in the shear stress vs. shear rate curve was an indication of sludge conditionablity, where the optimum polymer conditioning corresponding to the highest peak was obtained. However, few studies have addressed the effects of distinct conditioning factors on the appearance of peaks in such rheograms. In this study, the impact of factors such as high speed mixing and polymer dosing rates on the geometric and rheological characteristics of conditioned anaerobic digested sludge (ADS) with the polymer zetag7557 were investigated through the jar test method.The results showed that both the high speed mixing time and polymer dosing time had important effects on the emergence of the initial peak in test curves obtained using the Haake RV20 and Flokky rheometers for evaluation of conditioned ADS. A high speed mixing time within 60 s or a polymer dosing time of 5 s was sufficient for observation of the initial peak in flow curves, and both peak height and area decreased as the high speed mixing times were prolonged in most cases in this study. As same as the high speed mixing time, the extension of zetag7557 dosing time can also lead to the gradual decrease in the initial peak size of test curves, and form small aggregates with a decrease in two-dimensional fractal dimensions (D₂).Although the initial peak in the test curves was observed when high speed mixing intensities increased up to 300 rpm, there were several differences in the peak height and area observed on the Haake RV20 test rheograms and Floccky test curves. In addition, a high speed mixing intensity of 300 rpm was found to lead to the formation of smaller and less compact aggregates than other mixing intensities.All of the rheological and geometric parameters were somewhat correlated with fractal dimension-D_2P (based on regression analysis of the logarithmics of area and perimeter). However, D_2L(based on regression analysis of the logarithmics of area and maximum diameter) did not show good correlation with any other parameters. The median diameters of the aggregates were well correlated with one-dimensional fractal dimensions (D₁).     

Effects of lipids and oleic acid on biomass development in anaerobic fixed-bed reactors. Part II: Oleic acid toxicity and biodegradability

Oleic acid toxicity and biodegradability were followed during long-term operation of two similar anaerobic fixed-bed units. When treating an oleate based effluent, the sludge from the bioreactor that was acclimated with lipids during the first operation period, showed a higher tolerance to oleic acid toxicity (IC50 = 137 mg/l) compared with the sludge fed with a non-fat substrate (IC50 = 80 mg/l). This sludge showed also the highest biodegradation capacity of oleic acid, achieving maximum methane production rates between 33 and 46 mlCH4(STP)/gVS.day and maximum percentages of methanization between 85 and 98% for the range of concentrations between 500 and 900 mg oleate/l. When oleate was the sole carbon source fed to both digesters, the biomass became encapsulated with organic matter, possibly oleate or an intermediate of its degradation, e.g. stearate that was degraded at a maximum rate of 99 mlCH4(STP)/gVS.day. This suggests the possibility of using adsorption-degradation cycles for the treatment of LCFA based effluents. Both tolerance to toxicity and biodegradability of oleic acid were improved by acclimatization with lipids or oleate below a threshold concentration.     

Impact on reactor configuration on the performance of anaerobic MBRs: Treatment of settled sewage in temperate climates

The treatment efficiency and membrane performance of a granular and suspended growth anaerobic membrane bioreactor (G-AnMBR and AnMBR respectively) were compared and evaluated. Both anaerobic MBRs were operated in parallel during 250 days with low strength wastewater and under UK weather conditions. Both systems presented COD and BOD removal efficiencies of 80–95% and >90% respectively. Effluent BOD remained between 5 and 15 mgBOD L⁻¹ through the experimental period while effluent COD increased from 25 mg L⁻¹ to 75 mg L⁻¹ as temperature decreased from 25 °C to 10 °C respectively indicating the production of non biodegradable organics at lower temperatures. Although similar levels of low molecular weight organics were present in the sludge supernatant, recycling of the mixed liquor from the membrane tank to the bioreactor at a low upflow velocity enhanced interception of solids in the sludge bed of the G-AnMBR limiting the solid and colloidal load to the membrane as compared to the suspended system. Results from flux step test showed that critical flux increased from 4 to 13 L m⁻² h⁻¹ and from 3 to 5 L m⁻² h⁻¹ with gas sparging intensities varying from 0.007 m s⁻¹ to 0.041. Additional long term trials in which the effect of gas sparging rate and backwashing efficiency were assessed confirmed the lower fouling propensity of the G-AnMBR.     

The effect of reactor hydraulics on the performance of activated sludge systems—II. The formation of microbial products

A new model involving the concept of soluble residual microbial products formation was used to investigate the effect of reactor hydraulics on the substrate removal efficiencies of activated sludge systems. Strong experimental evidence in the literature suggests that what is measured in most studies is not the remaining portion of the influent degradable substrate, but organic matter of microbial origin which is residual, at least for the operating conditions considered. An appropriate simulation approach was formulated to account for the formation of these products, by a simple mechanistic modification of the newly proposed task group model. This model showed no practical difference between the performances of completely mixed and plug flow activated sludge systems, because they produced almost equal amounts of these microbial products, under similar operating conditions.     

Influence of transient substrate overloads on the proliferation of filamentous bacterial populations in an activated sludge pilot plant

Using oligonucleotide probes directed at the rRNA of filamentous bacteria, this study looks at the influence of the components of transient substrate overloads on the growth of the dominant filamentous bacteria of activated sludge fed by a synthetic substrate. By dissociating the massive input of organic matter from the oxygen shortage that the latter generally induces, it is revealed that each of these factors applied alone, induces only transitory, small-scale growth of the filaments Nostocoida limicola, Haliscomenobacter hydrossis. Thiothrix and of type 021N. In contrast, combining them during a reconstituted transient substrate overload with an artificially created oxygen deficit, induces very fast growth of H. hydrossis which is responsible for establishing major proliferation. This massive proliferation was easily reduced by chlorination.     

Biodegradation of nitrilotriacetate (NTA) by bacteria—II. Cultivation of an NTA-degrading bacterium in anaerobic medium

Bacterial strains with the ability to utilize nitrilotriacetate (NTA) under aerobic and anaerobic conditions have been isolated from natural waters exposed to NTA. One of these strains (NTA-A2) is a facultative anaerobe which grows under anaerobic conditions on NTA if nitrate is available in the medium. (Under aerobic conditions this strain can utilize acetate, glucose and some other sugars as well as NTA but not EDTA (ethylenediaminotetraacetate) or lactose as the sole carbon source.) The properties and characteristics of the strain NTA-A2 are described.     

Certification of the European Reference Soil Set (IRMM-443--EUROSOILS). Part II. Soil-pH in suspensions of water and CaCl2

IRMM-443 re-groups a set of six European Reference Soils (EUROSOILS), which had been certified for their adsorption coefficients for atrazine, 2,4-D and lindane (Certification of the European Reference Soil Set (IRMM-443-EUROSOILS)-Part I. Adsorption coefficients for atrazine, 2,4-D and lindane. Sci Total Environ, in press). The certification of these parameters was complemented by an additional certification of pH in suspension as well by the determination of indicative values for total nitrogen, organic and total carbon content. While Part I explained the principles of the value assignment process and discussed their application to the adsorption coefficients, Part II presents the certified values for pH as well as the indicative values for N(tot), C(tot) and C(org). In addition, the assessment of uncertainty components for stability and homogeneity, which have been included in the final uncertainty budget, is discussed.     

The effect of trace organics on the inhibition of gas production by anaerobic sludges: Batch studies

Gallic acid was used as a model inhibitor in a series of batch trials examining the anaerobic digestion of a substrate based on starch (1 g/l). Simple organic compounds were also added in trace (5 mg/l) quantities. The inhibition of digestion efficiency was assessed in terms of biogas production and gas composition. Some of the trace organics, specifically glucose and glycine, behaved antagonistically to the inhibitory effects of the gallic acid. The effect was quantified in terms of an activity term, based on the methane volumes produced. For glucose, its value was 56% and for glycine it was 38%. Altering the amounts of glucose did change the extent of the antagonism. In practical terms, the most effective concentration would be between 8 and 10 mg/l.     

The Biological System of the Elements (BSE). Part II: a theoretical model for establishing the essentiality of chemical elements. The application of stoichiometric network analysis to the biological system of the elements

Stoichiometric Network Analysis (SNA), originally developed by the Canadian chemist Bruce L. Clarke during the 1970s, provides a most efficient means of reducing the background topology of complex interaction networks to some skeleton topology around which systems dynamics can be understood without jeopardising insight into complex dynamics by over- or miss-simplification. Since it focuses on the corresponding autocatalytic (AC) features of a feedback system as those which control overall behaviour to some extent, SNA deals with reaction kinetics in and beyond chemistry, e.g. with nuclear reactions. It is therefore quite straightforward to apply this manner of simplification, which in turn is supported by a number of mathematical theorems on systems behaviour and properties of AC cycles, to biological systems although their 'full' complexity may not even be assessed in the yet rare cases of complete genetic sequencing. Assuming there is a relationship between the kinds of metal or metalloid species and key biological/biochemical transformations to be promoted with their aid--this relationship being the subject of bio-inorganic chemistry--and that biochemistry is, in effect, about systems which can reproduce and thus behave autocatalytically, one can expect SNA to yield formally sound statements on basic features of biology and biochemistry too. If we sum up the facts and considerations concerning essentiality or possible essentiality in a biological system of elements (Markert, 1994), this means joining the triangular representation of BSE, including statements on (the degree of biological) evolution and aggregation levels, to SNA treatment of autocatalysis within hierarchical systems from metalloenzymes to entire biocoenoses. Arguments using preferred cluster sizes and aggregation tendencies from coordination chemistry are then employed to circumscribe possible functions within the BSE. They are also extended to metals hitherto not known to be essential, such as tellurium or scandium.