Characteristics of bread making doughs: influence of sourdough fermentation on the fundamental rheological properties

The proofing process is an essential step in the bread making technology in providing a link between the bubble structure created in the mixer and the final baked loaf structure. This research was carried out to test the effect of two different types of fermentation on bread making dough properties. Fundamental rheological tests were used to evaluate and compare doughs prepared by using compressed yeast and only natural yeast. Dynamic oscillation tests were performed using a controlled stress--strain rheometer; the phase angle (δ) and the value of the storage modulus (G′) were measured for all samples. Significant differences were found between the doughs which were made using commercial compressed yeast (baker's yeast (BY)) and those prepared using sourdough (SD). In particular SD was less elastic, less firm and easily extensible having higher phase angle and lower G′.     

Combined effects of wheat gluten and carboxymethylcellulose on dough rheological behaviours and gluten network of potato–wheat flour-based bread

Incorporating high level of potato flour into wheat flour enhances nutritional values of bread but induces a series of problems that lead to the decline of the bread quality. To overcome the barrier, wheat gluten and carboxymethylcellulose (CMC) were added into potato–wheat composite flour to improve dough machinability and bread quality. The rheological properties, thermo-mechanical properties and microstructures of dough were investigated. The results showed that the interaction between gluten and CMC mitigated the discontinuity of gluten matrix and gluten protein aggregation caused by the addition of potato flour, which yielded a more branched and compact gluten network. The compact three-dimensional viscoelastic structure induced improvements of gas retention capacity and dough stability, making it mimic the machinability properties of wheat flour dough. Bread qualities were apparently improved with the combined use of 4% gluten and 6% CMC, of which specific volume increased by 42.86%, and simultaneously, hardness reduced by 75.93%.     

Impact of oleuropein on rheology and breadmaking performance of wheat doughs,and functional features of bread

Oleuropein (OP) is a polyphenol present in drupes and leaves of olive tree with health benefits and, as antioxidant, potentiality to alter gluten functionality. Effects of OP inclusion to wheat flour (0.01% and 0.02% on flour weight basis) on dough rheology and breadmaking performance, and bread features were investigated. Farinograph, uniaxial extension and shear rheometry (oscillatory and creep-recovery) were applied. Doughs containing OP were stronger, more elastic, and less sticky indicating the ability of OP to act as flour improver. The strengthening effect of OP on gluten led to the increase in bread volume and softer crumb compared to control. A lower crumb density of bread with the addition of OP was related to a higher in vitro glycaemic response. An increase in the antioxidant capacity of bread made with the phenolic compound was also found.     

Impact of whey protein addition on wheat bread fermented with a spontaneous sourdough

Wheat bread is consumed in large quantities throughout the world. However, it contains an inadequate amount of proteins. This fact can be improved by adding whey protein concentrate (WPC). Sourdough (S) fermentation generally improves some characteristics and the digestibility of bread. The aim of this paper was to determinate the physicochemical properties and in vitro protein digestibility of bread with 20% of WPC leavened with spontaneous S (WPC 20-S). To complete the experimental design, bread with S without WPC (WPC 0-S) and bread with yeast (Y) without and with WPC 20% (WPC 0-Y and WPC 20-Y, respectively) were produced. WPC 20-S bread had higher pH, lower moisture and higher hardness and chewiness, compared with WPC 0-S. Interestingly, WPC had a negative effect only on the specific volume of bread fermented with Y but not when S was used. For all formulations, WPC improved the protein content and in vitro protein digestibility.     

Microstructure,textural and sensorial properties of durum wheat bread as affected by yeast content

In this study, breads varying in yeast content and therefore leading to distinct cellular structures are investigated. Firstly, X-ray microtomography is used to characterize not only the final cellular structure, but also the process of development of microstructure during the fermentation stage. With μCT, image analysis of the full 3-D microstructure, measuring the size, shape, networking/connectivity and distribution of various phases was possible. These measurements represent the full 3-D microstructure, which is not always possible by 2-D image analysis using statistical techniques. Secondly, the textural properties of the baked bread were obtained by compression tests and finally, the sensorial quality of the final product is also evaluated and correlated with the above properties. Results showed that an increase of the yeast content increased the percentage volume of pores and decreased the force required for compression of the bread sample and the tenacity. Moreover, a larger quantity of smaller sized pores led to a firmer bread structure. Regarding the sensorial properties, none of the investigated microstructural parameters were significantly correlated with the overall quality of the bread. In fact, the overall quality was more strongly affected from other parameters such as odor and appearance attributes.     

Impact of sourdough on the texture of bread

Sourdough has been used since ancient times and its ability to improve the quality and increase the shelf-life of bread has been widely described. During sourdough fermentation, lactic acid bacteria (LAB) produce a number of metabolites which have been shown to have a positive effect on the texture and staling of bread, e.g. organic acids, exopolysaccharides (EPS) and/or enzymes. EPS produced by LAB have the potential to replace more expensive hydrocolloids used as bread improvers. Organic acids affect the protein and starch fractions of flour. Additionally, the drop in pH associated with acid production causes an increase in the proteases and amylases activity of the flour, thus leading to a reduction in staling. While improving the textural qualities of bread, sourdough fermentation also results in increased mineral bioavailability and reduced phytate content. In this review we will be discussing the effect of sourdough on wheat and rye bread as well as the potential of sourdough to improve the quality of gluten-free bread.     

The polypeptide composition,structural properties and antioxidant capacity of gluten proteins of diverse bread and durum wheat varieties,and their relationship to the rheological performance of dough

The aim of this study was to compare five bread and five durum wheat genotypes for gliadins and glutenins profiles, the concentration of free sulphhydryl groups and disulphide bonds, antioxidant capacity of gluten proteins and their bread‐making performance. On average, bread wheat had significantly higher concentration of total sulphur‐rich (S‐rich) and sulphur‐poor (S‐poor) subunits of gliadins, as well as total low molecular weight (LMW) and high molecular weight (HMW) subunits of glutenins than durum wheat. However, durum wheat had higher concentration of S‐rich γ‐gliadins and S‐poor D‐LMW‐glutenins, but did not possess S‐poor ω‐gliadins. The concentration of disulphide bonds and total cysteine was higher in the durum gluten than that in the bread gluten, as well as antioxidant capacity (on average 90.6 vs. 85.9 mmol Trolox Eq kg^?1, respectively). In contrast to the bread wheat, the concentration of HMW‐glutenins was negatively associated with extensibility, as well as resistance to extension in durum wheat flour dough.     

Shelf-life stability of artisanally and industrially produced durum wheat sourdough bread (“Altamura bread”)

Physico-chemical properties and volatile compounds of three commercial Altamura breads were evaluated during storage at 25 °C. Two protected denomination of origin (PDO) artisanally produced Altamura breads (Bari, Italy), characterized either by high (High A) or low (LowA) loaf, and an industrial product, commercialized as “Altamura like” (IndA), were studied.HighA and LowA breads had a tick crust that was also detached from the crumb creating an air cushion between crust and crumb. IndA products had a thinner crust, a more homogeneous crumb structure as well as a more homogeneous water distribution among the different portion of the bread loaf than HighA and LowA. A more pronounced water gradient characterized the artisanal breads. Crust and under crust portion of all breads, and crumb for IndA product, underwent a significant reduction of moisture content and a_w during storage. Both artisanal breads were subjected to a more significant crumb hardening than IndA sample. Fresh crusts of artisanally produced breads were also significantly harder than IndA. Fresh IndA samples were significantly less cohesive and less springy than artisanal products; cohesiveness significantly decreased in all samples during storage. A more complex gas chromatographic profile was found in the artisanal bread as a larger amount of volatile compounds was present as compared to the IndA bread. Volatile compounds originated both from microbial activity and non-enzymatic browning. Larger amount of volatile compounds characteristics of yeast fermentation was found in IndA. Volatiles decreased over storage in both samples, more significant in the IndA product.     

Effect of durum wheat varieties on bread quality

In this study, the effect of different durum wheat varieties on the sensorial and nutritional quality of bread was assessed. In particular, bread manufactured with six wheat cultivars was compared with bread based on commercial semolina mixtures. X‐ray microtomography was used to characterise the final cellular structure of the baked bread. The textural properties of baked bread and doughs were also investigated. In addition, the glucose equivalent (GE) of bread was also evaluated. Finally, the microstructural, sensorial and textural parameters were correlated with each other. Results highlighted that Cappelli and Saragolla bread samples showed higher protein and total soluble fibre content, respectively, in comparison with the reference samples. Moreover, the GE of the Cappelli, Claudio and Saragolla bread samples was similar to that of the reference samples and lower than that of the other samples. However, the sensorial properties of all bread samples showed a very positive score (up to 7.7).     

Effects of sourdough and enzymes on staling of high-fibre wheat bread

The effects of sourdough and enzyme mixture (α-amylase, xylanase and lipase) on the specific volume, staling and microstructure of wheat pan bread supplemented with wheat bran were studied. Staling of bread was followed for 6 days by measuring the crumb firmness, changes in crystallization of amylopectin (DSC), increase in signal from the solid phase (NMR) and by light microscopy. The most effective treatment in improvement of quality was the combination of bran sourdough and enzyme mixture. During storage the rate of changes in crumb firmness, amylopectin crystallinity and rigidity of polymers were greatest for the white wheat bread. The most pronounced microstructural changes were swelling of starch granules and separation of amylose and amylopectin in the starch granules. Least changes in crumb firmness, amylopectin crystallinity and rigidity of polymers were observed in bran sourdough bread with enzymes. In contrast to white wheat bread, the starch granules were very much swollen in bran sourdough bread with enzyme mixture. This was hypothesized to be due to the higher water content of bran bread, and degradation of cell wall components leading to altered distribution of water among starch, gluten and bran particles during storage.     

The effects of wheat sourdough on glutenin patterns, dough rheology and bread properties

Sourdough was prepared with cellular suspension containing 10⁹ cfu of each strain mL⁻¹ and incubated at 28 °C for 24 h and at 37 °C for 4 h. Two different sourdough levels (20 and 40%) were used in bread dough preparation. The bread doughs were proofed at 30 °C and 85% relative humidity for 60/120/180 min. When glutenin changes that occurred in samples 17, 18, 19, and 20 (40% SD 28) are compared with those that appeared in controls, it is obvious that, the relative intensities of some of the protein bands slightly decreased and a few fainter protein bands appeared (which did not exist in controls). A few fainter protein bands corresponding to the MM ≈ 25 kDa (high-mobility region) and the MM ≈ 66 kDa (low-mobility region) were appeared in the same samples. In the samples prepared with 20% sourdoughs incubated at 28 or 37 °C, the bands were still evident after 180 min of proof. This can be explained that glutenin fractions were not hydrolysed in these applications due to the delay in pH drop. The use of 40% sourdough incubated at 28 °C for 24 h resulted in sticky doughs and breads with lower volume, harder texture, unsatisfactory crumb grain and unpleasant flavour than the rest of the samples. The use of sourdoughs incubated at 37 °C for 4 h caused positive effect on loaf volumes, specific loaf volumes and crumb structure.     

蕨麻对面包制品品质特性的影响

主要研究面粉中添加1％～9％的蕨麻粉对面团粉质特性以及面包品质的影响。结果表明．随着蕨麻粉添加量的增加,面团的吸水率逐渐增高,面团的形成时间和稳定时间延长,弱化度降低;随着蕨麻粉添加比例的增加,面包硬度增加,面包的感官评分总体上呈现降低趋势,但是当蕨麻添加量大于5％时,面包体积急剧减少,面包纹理结构变差,质地和口感都明显下降,因此,蕨麻粉在面包粉中的合理添加比例为5％。     

The sourdough microflora Microbiological, biochemical and breadmaking characteristics of doughs fermented with freeze-dried mixed starters, freeze-dried wheat sourdough and mixed fresh-cell starters

Freeze-dried mixed starters, freeze-dried wheat sourdough and mixed fresh-cell starters made withLactobacillus sanfrancisco CBI,L. plantarum DC400 andSaccharomyces cerevisiae 141 and/orS. exiguus M14 were used for leavening wheat doughs, and their microbiological, biochemical and breadmaking characteristics were compared with those of Italian traditional doughs produced by baker's yeast. All the doughs fermented with starters had more balanced microbiological and biochemical characteristics than dough started with baker's yeast in which alcoholic fermentation end-products largely predominated. By using starters, the greatest lactic acid bacteria cell number and acetic acid production, were achieved, along with more complete profiles of volatile compounds and greater structural stability of fermented doughs. Fresh-cell starters showed higher microbial functionality and represented the only way to enrich the doughs withS. exiguus M14, some of which survived the freeze-drying process. No differences were detected between the two different types of freeze-dried starters and the subsequent use (10 times) of doughs initially produced with freezedried starters eliminated initial differences in the microbial functionality with respect to fresh-cell starters.     

Effects of wheat sourdough process on the quality of mixed oat-wheat bread

The aim of this work was to study the effects of sourdough fermentation of wheat flour with Lactobacillus plantarum, on the quality attributes of mixed oat-wheat bread (51 g whole grain oat flour and 49 g/100 g white wheat flour). Emphasis was laid both on β-glucan stability as well as bread structure and sensory quality. The variables of the sourdough process were: dough yield (DY), fermentation time, fermentation temperature, and amount of sourdough added to the bread dough. The sourdough process was shown to be a feasible method for mixed oat-wheat bread, and, when optimized, provided bread quality equal to straight dough baking. A small amount (10g/100 g dough) of slack sourdough fermented at high temperature for a long time resulted in the most optimal sourdough bread with the highest specific volume (3.5 cm³/g), the lowest firmness after 3 days storage (0.31 kg), and low sensory sourness with high intensity of the crumb flavour. Wheat sourdough parameters did not affect the content of oat β-glucan in the bread. Additionally, both straight dough and sourdough bread contained 1.4-1.6 g β-glucan/100 g fresh bread. The average molecular weight of β-glucan was 5.5 × 10⁵ in both types of bread, while that of oat flour was 10 × 10⁵. This indicates that a slight degradation of β-glucan occurred during proofing and baking, and it was not affected by variation in the acidity of the bread between pH 4.9-5.8.     

Model studies for wheat sourdough systems using gluten,lactate buffer and sodium chloride

Model studies were conducted in order to study the influence of acid and NaCl, as occurring in wheat sourdough bread, on fundamental rheological properties of wheat gluten. Gluten was divided into pieces and subjected to a swelling period in lactate buffer of pH 3.9, with or without added NaCl (3 g/100 ml). The respective controls were unbuffered NaCl solution and pure water. The microstructure of the gluten pieces was studied by laser-scanning confocal microscopy and the recombined pieces were examined using fundamental rheology. The combination of buffer (pH 3.9) and NaCl in comparison to unbuffered NaCl solution caused a denser, but partially dissolved fibrillar microstructure. Further to this, swelling of the gluten was reduced (62.0% versus 65.9% moisture) and an increase in firmness and elasticity was observed: in comparison with unbuffered NaCl solution, the absolute value of the complex dynamic modulus (|G*|) was higher, while the phase angle was lower in dynamic oscillatory measurements at 30 °C and dynamic temperature sweeps (30–95 °C), while in creep tests at 30 °C and 95 °C strain values were lower and relative recovery higher. In contrast, pH 3.9 buffer without added NaCl caused softer rheological behaviour than water and a film-like microstructure.     

Significance of viscosity profile of pasted and gelled formulated wheat doughs on bread staling

Pasting profile during cooking and cooling of straight/soured started bread doughs formulated with non fat-sodium carboxymethylcellulose (CMC), hydroxypropylmethylcellulose (HPMC), fungal α-amylase and fat-monoglycerides (MGL), diacetyl tartaric acid ester of mono-diglycerides and sodium stearoyl lactylate (SSL)-additives was recorded in the Brabender (BVA) visco-amylograph and Newport rapid viscoanalyser (RVA). Rheological results were correlated with bread staling kinetics during storage. Bread dough viscosity characteristics, derived from the RVA pasting profile during cooking and cooling, highly correlate with bread staling kinetic parameters. This is particularly so in the cases of peak viscosity, pasting temperature, and setback during cooling that can be considered as valuable predictors, at a dough level, of bread firming behaviour during storage. Individual and/or binary addition of surfactants to bread dough, particularly MGL and SSL, positively influence the level of the pasting parameters associated with a significant delay in bread firming. Individual additions of methylcellulose derivatives, mainly CMC, induce in general a deleterious effect on dough viscosity. Moreover, the simultaneous presence of CMC and HPMC results in a significant improvement of dough rheology during cooling. Binary mixtures SSL/CMC and MGL/CMC are not recommended from the viscoelastic point of view, due to antagonistic effects of the pair gum/surfactant that nullify the benefits of individual emulsifiers.     

小麦粉面筋蛋白质流变学特性的研究

本试验采用快速黏度仪(RVA)通过面筋蛋白醇溶指数法,研究了不同类型小麦粉的面筋蛋白醇溶指数,探讨了不同小麦粉的粉质特性、拉伸特性及湿面筋、面筋指数及其与醇溶指数、最终黏度的影响.结果表明:不同类型的小麦粉的面筋蛋白RVA曲线存在明显差异,醇溶指数在α=0.05水平上与小麦粉吸水率、延伸性呈负相关,与峰值黏度呈正相关;在α=0.10水平上与小麦粉稳定时间呈负相关,最终黏度在α=0.05水平上与小麦粉面筋指数呈负相关,与峰值黏度呈正相关.因此,麦胶蛋白明显影响小麦粉的吸水率、稳定时间、延伸性及面团的面筋指数,而与拉伸阻力、湿面筋及弱化度无相关性,此与麦胶蛋白的富有延伸性、粘性,但弹性小的性质有关.     

Heat damage of water biscuits from einkorn,durum and bread wheat flours

To limit heat damage and improve the nutritional properties of bakery products, furosine, glucosylisomaltol, hydroxymethylfurfural, furfural, sugars, α-amylase, β-amylase and colour were assessed during the production of water biscuits from three einkorn, three bread and one durum wheat flours. Heat damage indices, colour and a_w development during baking (from 25 to 75 min duration) of water biscuits from one bread wheat were also studied.