Effect of time of measurement on the relationship between metmyoglobin reducing activity and oxygen consumption to instrumental measures of beef longissimus color stability

Contributions of initial and retained levels of oxygen consumption and reducing capacity to animal variation in color stability were evaluated. Instrumental color values were determined on longissimus steaks (n=257) during 6 d of display. Oxygen consumption (OC), nitric oxide metmyoglobin reduction (NORA), initial metmyoglobin formation (IMF), and post-reduction metmyoglobin (PRM) were measured on d 0 and 6. During display, color variables, OC and reducing ability decreased (P<0.05). Color stable steaks had greater (P<0.05) reducing ability on d 0 and 6 and lower (P<0.05) OC on d 0 than unstable steaks. Color change was correlated to OC, NORA, and PRM on d 0 (r=0.19, -0.44 and 0.45, respectively) and to NORA and PRM on d 6 (r=-0.50 and 0.52, respectively). These data suggest that initial capacity for OC and reducing ability, combined with retained reducing ability contribute to animal variation in color stability.     

Electrical Stimulation Effects on Myoglobin Properties of Bovine Longissimus Muscle

Beef longissimus steaks from 40 bull carcasses were used to study the effects of electrical stimulation (ES) on meat color mechanisms and myoglobin properties. Total pigment concentration and ratios of myoglobin forms for ES and control (C) samples were not different. Color panel and several spectrophotometric measurements indicated ES produced a lighter, brighter-red color in displayed steaks. Disappearance of reduced myoglobin during blooming was similar in unaged C and ES steaks, but reduced myoglobin decreased faster during blooming of ES steaks aged 6 days. ES decreased anaerobic metmyoglobin reducing activity and induced more metmyoglobin formation when steaks were in a 1% oxygen atmosphere.     

Effects of Beef Carcass Electrical Stimulation and Hot Boning on Display Color of Unfrozen Vacuum Packaged Steaks

Ninety-six beef sides from 48 carcasses were used to determine effects of control (C, chilled 48 hr at 5°C), electrical stimulation (ES, 45 min postmortem, 400 volts for 2 min, pulsed), and hot boning (HB, 2 hr postmortem), and combination (ESHB) treatments on muscle color of longissimus (LD) and semimembranosus (SM), vacuum packaged steaks. HB muscles frequently were visually brighter purplish-red than other treatments. Compared to ESHB, ES LD was not different, but ES SM was duller purplish-red in color. Reflectance indicators of reduced myoglobin and metmyoglobin were essentially the same across treatments in both muscles. Vacuum packaged fresh beef steaks from all treatments were acceptable in color at 0, 3, 7, and 14 days of display. Vacuum packaging appears suitable for steaks from any of these carcass treatments but is especially useful for steaks from hot boned cuts.     

Mitochondrial Activity and Beef Muscle Color Stability

Concentration, oxygen consumption rate (OCR), oxygen consumption rate/g meat (OCRM) and metmyoglobin reducing activity (MRA) of muscle mitochondria were determined for beef longissimus dorsi and gluteus medius muscles of Holstein and crossbred steers which differed in color stability. OCR and OCRM decreased but no significant changes were observed in MRA during postmortem storage time. Significant effects of muscle and breed type on OCR, OCRM, and MRA were observed. Muscles and breeds of lower color stability had the highest levels of OCR, OCRM, and MRA. Differences in OCR of muscle mitochondria may be a contributing factor in the effects of muscle and breed on the rate of discoloration.     

注射水和氯化钙溶液对宰后猪肉肉色及其稳定性的影响

目的：探讨注射水和氯化钙溶液对宰后冷藏期间猪背最长肌肉色及其稳定性的影响。方法：猪背最长肌于宰后1.5h注射肉质量分数5%的水和200mmol/L氯化钙溶液,分别测定其冷藏期间肉色a*值、总色素含量、高铁肌红蛋白(MetMb)相对百分含量、MetMb还原酶活性等指标。结果：注射氯化钙溶液能降低肉中a*值、总色素含量、MetMb还原酶活性和乳酸脱氢酶-B(LDH-B)活性,增加MetMb相对百分含量和丙二醛(MDA)含量。注水能降低肉中总色素含量和MetMb还原酶活性,增加MetMb相对百分含量。结论：注射水和氯化钙溶液均能降低宰后冷藏期间猪背最长肌中总色素含量,增加MetMb相对百分含量,从而加快猪背最长肌的褪色,不利于其冷藏期间新鲜肉色的维持。     

Effects of Beef Carcass Electrical Stimulation and Hot Boning on Muscle Display Color of Polyvinylchloride Packaged Steaks

Ninety-six beef sides from 48 carcasses were used to determine the effects of control (C, chilled 48 hr at 5°C), electrical stimulation (ES, 45 min postmortem, 400 volts for 2 min, pulsed), hot boning (HB, 2 hr postmortem), and combination (ESHB) treatments on muscle color of longissimus (LD) and semimembranosus (SM) steaks packaged in polyvinylchloride film. LD from HB was mostly visually darker, had less oxymyoglobin, and more metmyoglobin than other treatments as was the SM, but SM had fewer differences between HB and ESHB. ES and ESHB muscles were visually similar, suggesting ES minimized the darkening effect of HB. Regardless of treatment, muscle color was acceptable at 0, 1, 3 and 5 days of display.     

牛背最长肌高铁肌红蛋白还原酶的分离及其酶学性质

以冷鲜牛背最长肌为原料,采用硫酸铵盐析、超滤法对原料肉中的高铁肌红蛋白还原酶进行粗分离,并研究温度、pH、金属离子对高铁肌红蛋白还原酶活性的影响.结果表明:在硫酸铵饱和度65％,超滤(＞50 kDa)条件下,牛背最长肌高铁肌红蛋白还原酶可得到分离与纯化.试验范围内,牛背最长肌高铁肌红蛋白还原酶的分子量为29.0～66....     

高氧气调包装贮藏牛排肉色稳定性的蛋白质组学

为进一步了解高氧气调包装牛排贮藏过程中肉色稳定性变化的机理,本研究比较了腰背最长肌牛排在各贮藏时间点（0、5、10、15?d）的肉色指标变化以及蛋白质组学变化。结果发现高氧气调包装牛排的肉色稳定性随贮藏时间延长不断降低,这可能是贮藏期间牛排的高铁肌红蛋白还原能力和氧气消耗率显著下降所致;通过蛋白质组学分析,共发现了20?个在贮藏过程中发生显著变化的差异蛋白,其中有15?个蛋白与肉色密切相关,它们主要是一些参与糖酵解和能量代谢的酶类（丙酮酸激酶、3-磷酸甘油醛脱氢酶、果糖-二磷酸醛缩酶、果糖-1,6-二磷酸酶同工酶2、苹果酸脱氢酶和黄素还原酶）,这些代谢酶的表达量随贮藏时间延长发生了不同程度的下调,减少了烟酰胺腺嘌呤二核苷酸和还原型烟酰胺腺嘌呤二核苷酸磷酸的产生,进而降低了肉色稳定性;另外,高氧气调包装贮藏过程中肉色稳定性的降低可能也与肌红蛋白的表达量下调有关;高氧环境还可能会促使抗氧化蛋白2、热休克蛋白以及DJ-1蛋白的表达量上调以抵御肉品的氧化应激反应,这些蛋白也都与肉色指标紧密相关。这些发现表明贮藏期间高氧气调包装对牛排蛋白质组的影响将直接决定其肉色稳定性的变化。     

Dietary vitamin E effect on color stability and sensory assessment of spoilage in three beef muscles

Effects of dietary vitamin E supplementation (1204 IU/head/day) for 122 days on color stability and microbial load on beef m. longissimus lumborum (LL), m. gluteus medius (GM) and m. psoas major (PM) were studied by subjective and objective evaluation. Color stability of these muscles followed the order LL > GM > PM (p < 0.05). Vitamin E-treated LL, GM and PM showed less metmyoglobin formation, higher a^* values and lower hue angle values than controls during storage at 4 °C (p < 0.05). Sensory evaluation demonstrated that panelists preferred the appearance of vitamin E-treated LL, GM and PM beef steaks. Vitamin E supplementation did not affect total microbial load on LL, PM and GM and did not influence panelists' olfactory assessment of microbial spoilage of beef. Endogenous -tocopherol concentration and lipid stability of microsomal fractions of LL, GM and PM were greater (p < 0.05) in vitamin E-treated muscles relative to controls. There was no muscle effect on the pro-oxidant activity of microsomes towards oxymyoglobin oxidation (p > 0.05). Oxymyoglobin stability was greater in the presence of microsomal fractions obtained from vitamin E-treated muscle than in those from controls. Dietary vitamin E supplementation delayed oxymyoglobin oxidation in LL, PM and GM muscle and increased the color shelf-life of these muscles without affecting total microbial load.     

Color and Color Stability of Frozen Restructured Beef Steaks: Effect of Processing Under Gas Atmospheres with Differing Oxygen Concentration

Restructured beef steaks were manufactured under gas atmospheres with differing oxygen concentration and subsequently vacuum-packaged. The effect of the different gas atmospheres on the color and color stability of frozen restructured beef steaks initially and at 1-month intervals for three months was investigated. Overall metmyoglobin concentrations for restructured beef steaks were not different (P > 0.05) from that of the intact steaks either initially or over storage time. The rates of overall metmyoglobin formation increased when oxygen concentration of the gas atmosphere was increased. Restructured steaks manufactured using pure carbon dioxide gas had the least amount of overall discoloration both initially and over storage time.     

Improvement of Color and Lipid Stability in Beef Longissimus with Dietary Vitamin E and Vitamin C Dip Treatment

The effects of dietary vitamin E supplementation and vitamin C dip treatment on color and lipid stability in longissimus muscle from Holstein and crossbred beef steers were studied during 16 days of display at 4°C. Dietary vitamin E supplementation retarded metmyoglobin formation of the meat and highly suppressed lipid oxidation compared to the control. Holstein longissimus showed higher metmyoglobin formation than crossbred beef longissimus. Dip treatment with a vitamin C solution was effective in maintaining stability of beef color and lipid.     

Color and Its Stability in Restructured Beef Steaks during Frozen Storage: Effects of Various Binders

Color and its stability were evaluated in restructured steaks made with various binders (calcium alginate, crude myosin extract, whey protein concentrate, wheat gluten, soy isolate or surimi) vs controls (intact ribeye muscle, restructured steaks with no additives or with NaCl and sodium tripolyphosphate). Steaks made with various binders showed similar effects on initial surface metmyoglobin concentration and sensory color attributes, except steaks made with calcium alginate or soy isolate protein. During 12-wk frozen storage, steaks made with various binders (except soy protein isolate) had similar color stabilities.     

EFFECT OF POSTMORTEM CONDITIONS ON CERTAIN CHEMICAL, MORPHOLOGICAL AND ORGANOLEPTIC PROPERTIES OF BOVINE MUSCLE

Paired sides from U.S. Choice grade beef were aged immediately after slaughter at 2 and 16°C. Samples were removed from longissimus and semitendinosus at slaughter and at 1, 3 and 7 days postmortem for ATPase assay, phase microscopy, shear and organoleptic evaluation. Rib steaks from sides aged at 16°C for 1-day postmortem were as tender as steaks from sides aged at 2°C for 7 days postmortem. Flavor development of rib steaks also was more rapid at 16°C than at 2°C. Tenderness of semitendinosus steaks was improved by aging sides at 16°C; the difference in improvement of tenderness of semitendinosus, however, was not as great between 2°nd 16° as it was for rib steaks. Ca⁺⁺, Mg⁺⁺ and EGTA-modified ATPase activity of myofibrils from both muscles increased with postmortem time, with myofibrils from muscles held at 16°C having slightly higher ATPase activity than myofibrils from muscles held at 2° Increased EGTA-modified ATPase activity was indicative of loss of calcium sensitivity of the myofibril. Sarcomeres of myofibrils from longissimus were longer at 1-day postmortem than those from at-death longissimus and they remained essentially unchanged during the remainder of postmortem aging; however, tenderness improved at 16°C for 1 day and at 2°C for 3 days. Also greater fragmentation of myofibrils from longissimus postmortem aged at 16°C for 1 day and at 2°C for 3 days was observed, suggesting that the rate of myofibril fragmentation is an important factor in tenderization.     

Microbiological and sensory characteristics of beef loin steaks: Role of intramuscular fat

Beef loin steaks of different grades (Prime, Choice and Good) were packaged and stored in polyvinyl chloride (PVC) film for 0-6 days and in high-oxygen barrier (HOB) film for 0-28 days. Grade had no significant effect (P > 0·05) on the aerobic plate count and did not result in major differences in the distribution of types in the microflora of steaks. Of the sensory characteristics examined, mean surface discoloration and mean overall appearance scores of Prime and Choice steaks packaged and stored in HOB film often were higher (P < 0·05) than those of Good steaks. Differences in metmyoglobin formation among steaks from the three grades were attributed to differences in the inherent characteristics of the muscles; muscle fibers from Prime and Choice samples were probably more red (as evidenced by greater marbling ability), while muscle fibers of Good samples were probably more white (as evidenced by lesser marbling ability). Red muscles have greater cytochrome activity, which will help reduce metmyoglobin to myoglobin in the absence of oxygen.     

紫外照射和温度波动对冷鲜肉肉色稳定性的影响

以不同部位冷鲜猪肉(背最长肌、后腿肉、腰大肌)为对象,研究肉样在2种贮藏条件(实际销售贮藏条件:4℃-13 h-紫外照射,0℃-11 h-避光;实验室设置条件:4℃-24 h-紫外照射)下色泽、肌红蛋白含量、高铁肌红蛋白还原酶活等的变化,旨在为提高冷鲜肉在销售贮藏期间的肉色稳定性提供依据。结果表明:实验室条件下,3个部位(背最长肌、后腿肉、腰大肌)冷鲜猪肉的色泽感官评定、a*值、氧合肌红蛋白含量和高铁肌红蛋白还原酶活性等均显著优于实际销售条件(P<0.05),实验室条件下更利于不同部位冷鲜猪肉的贮存。另外,在实验室条件下,3个部位中猪背最长肌肉色最稳定,其次是后腿肉,腰大肌肉色稳定性最差。     

Metmyoglobin Reducing Capacity of Fresh Normal, PSE, and DFD Pork During Retail Display

Fresh pale, soft, exudative (PSE), dark, firm, dry (DFD), and normal pork were stored under light or dark conditions at 4°C for 7 days. Sample pH, metmyoglobin reductase activity, oxygen consumption rate, and relative surface metmyoglobin and oxymyoglobin contents were determined. DFD pork had the highest metmyoglobin reductase activity and oxygen consumption rate. Enzyme activity of PSE was lower than that of normal pork, but no difference existed in oxygen consumption rate between PSE and normal samples. Metmyoglobin reductase activity dropped slowly during meat storage; oxygen consumption rate sharply decreased during the first day of storage. Both metmyoglobin reductase activity and oxygen consumption rate declined more rapidly in the light. Results can help develop guidelines for display and packaging of pork.     

Biochemical and physical factors affecting discoloration characteristics of 19 bovine muscles

Steaks from muscles (n = 19 from nine beef carcasses) were evaluated over the course of retail display (0-, 1-, 2-, 3-, 4- or 5-d) for objective measures of discoloration (metmyoglobin, oxymyoglobin, L*-, a*-, and b*-values), reducing ability (metmyoglobin reductase activity (MRA), resistance to induced metmyoglobin formation (RIMF), and nitric oxide metmyoglobin reducing ability (NORA)), oxygen consumption rate (OCR), oxygen penetration depth, myoglobin content, oxidative rancidity, and pH. Muscles were grouped according to objective color measures of discoloration. M. longissimus lumborum, M. longissimus thoracis, M. semitendinosus, and M. tensor fasciae latae were grouped as “high” color stability muscles, M. semimembranosus, M. rectus femoris, and M. vastus lateralis were grouped as “moderate” color stability muscles, M. trapezius, M. gluteus medius, and M. latissimus dorsi were grouped as “intermediate” color stability muscles, M. triceps brachi – long head, M. biceps femoris, M. pectoralis profundus, M. adductor, M. triceps brachi – lateral head, and M. serratus ventralis were grouped as “low” color stability muscles, and M. supraspinatus, M. infraspinatus, and M. psoas major were grouped as “very low” color stability muscles. Generally, muscles of high color stability had high RIMF, nitric oxide reducing ability, and oxygen penetration depth and possessed low OCRs, myoglobin content, and oxidative rancidity. In contrast, muscles of low color stability had high MRA, OCRs, myoglobin content, and oxidative rancidity and low RIMF, NORA, and oxygen penetration depth. Data indicate that discoloration differences between muscles are related to the amount of reducing activity relative to the OCR.     

Effects of succinate and pH on cooked beef color

Two experiments were conducted to assess the effects of succinate and pH on cooked beef color. In experiment 1, ten strip loins (M. longissimus lumborum) were divided in half and assigned to either non-enhanced control or 2.5% succinate. Each half-loin was cut into steaks, packaged in vacuum or 80% oxygen, and stored at 1 °C for 0, 6, or 12 days. Steaks were cooked to either 66 °C or 71 °C. Succinate increased (P < 0.05) steak pH, raw a* values, and interior cooked redness when packaged in high oxygen. In experiment 2, to assess the role of succinate in raw and cooked color, succinate or ammonium hydroxide was added to ground beef patties to result in a common meat pH (5.9). At a similar pH, succinate had greater metmyoglobin reducing activity and internal cooked redness compared with ammonium hydroxide (P < 0.05). In addition to ingredient-based changes in muscle pH, succinate may influence color by regenerating reducing equivalents.     

Effect of dietary β-carotene supplementation on beef color stability during display of two muscles from Japanese Black steers

Effect of dietary β-carotene supplementation (7500 mg/head/day) for 28 days prior to slaughter on beef color stability during display of M. semimembranosus (SM) and M. longissimus lumborum (LL) from Japanese Black steers was studied. Steak samples from two muscles were over-wrapped with PVC film and displayed under fluorescent lights at 4°C for 12 days. Metmyoglobin percentages of steak samples were determined at days 0, 3, 6, 9 and 12. The β-carotene concentration in both muscles was increased (P<0.001) by dietary β-carotene supplementation. Color display-life of muscles was calculated by the metmyoglobin threshold method based on a threshold value of 20% metmyoglobin. Color display-lives of SM and LL were extended 1.5 and 3 days by dietary β-carotene supplementation, respectively.     

Metmyoglobin reducing activity and colour stability of ovine longissimus muscle

Characteristics of metmyoglobin reducing activity in ovine longissimus were determined, and its effect on colour and colour stability of muscle was investigated in two experiments. In the first experiment vacuum packed ovine longissimus samples were incubated at 5–35°C during the first 16 h post mortem (n=8 per treatment). Metmyoglobin reducing activity was negatively affected by incubation temperatures above 30°C, but colour and colour stability were little affected at 24 h post mortem and after 2 weeks of vacuum storage at 2°C. In the second experiment the effects of pre-slaughter stress and electrical stimulation on metmyoglobin reducing activity, colour and colour stability of ovine longissimus (n=40) with an ultimate pH below 5.8 were investigated. Neither of the treatments had an effect on metmyoglobin reducing activity or colour parameters. The relatively large variation in metmyoglobin activity and colour parameters allowed correlation analysis. Metmyoglobin reducing activity was not correlated to colour or the colour stability parameters. The results of the present study indicate that metmyoglobin reducing activity is not the primary determinant of colour or colour stability of ovine longissimus muscle.