共查询到20条相似文献,搜索用时 62 毫秒
1.
2.
研究了麦芽协同复合蛋白酶水解玉米蛋白粉的条件,并分析了水解物的抗氧化活性。结果表明:最佳条件为先用麦芽水解,在底物质量浓度150 g/L、麦芽添加量15 g/100g、pH 5.5、温度50℃条件下水解3 h;然后用复合蛋白酶水解,在加酶量1.3 g/100 g、pH 8.0、温度60℃条件下水解2.5 h。在此条件下,蛋白质回收率为50.18%±0.24%,相比于不加麦芽,蛋白质回收率提高了11.16%。水解物蛋白质量浓度为1 mg/mL时,亚铁离子螯合率、DPPH·清除率、·OH清除率和·O-2清除率分别为83.90%±0.38%、51.19%±1.07%、79.30%±0.63%和35.68%±0.51%,相比于不加麦芽,亚铁离子螯合率降低0.85%,DPPH·清除率、·OH清除率和·O-2清除率分别提高了8.13%、24.41%和64.73%。 相似文献
3.
4.
以玉米胚芽蛋白水解物为底物,通过对其羟基自由基清除能力、超氧阴离子自由基清除能力、二苯基苦味酸基苯肼(DPPH)自由基清除能力、Cu2+螯合能力、在亚油酸体系中的抗氧化性、油脂过氧化值(POV)等指标的测定,研究玉米胚芽蛋白水解物的抗氧化活性.结果表明,玉米胚芽蛋白水解物对羟基自由基、超氧阴离子自由基、DPPH自由基具有一定的清除能力,且浓度为6mg/mL时,清除效果最好;对Cu2+具有螯合能力,并且螯合能力随着玉米胚芽蛋白水解物浓度的增加而呈上升趋势;对亚油酸氧化具有抑制作用,作用效果与剂量成正相关,同时对油脂的POV值具有抑制作用,浓度为0.1%时抑制作用最强. 相似文献
5.
目的:研究黄粉虫酶解物(TMH)体内外抗氧化活性和免疫活性。方法:研究TMH的还原能力及其对DPPH自由基和超氧阴离子自由基的清除能力;通过动物试验研究TMH的体内抗氧化能力和免疫能力。结果表明:TMH具有较强的抗氧化能力,对DPPH自由基、O_2~-·自由基的清除能力分别达到88.4%(10 mg/mL),61.0%(200 mg/L),还原能力为0.653(6 mg/L);动物试验表明,TMH可以显著提高小鼠血浆、肝脏中CAT、GSHPx、T-SOD(P0.05)活力,降低MDA(P0.05)含量并抑制羟自由基能力;显著增加血清中IgG、IgM、IL-4、IFN-γ(P0.05)的含量,提高小鼠脾脏和肝脏指数,表现出较强的抗氧化能力以及改善机体免疫功能的作用。 相似文献
6.
7.
8.
玉米蛋白肽的生产加工研究现状 总被引:1,自引:0,他引:1
玉米蛋白粉是生产玉米淀粉的副产物,因其色泽为玉米黄,又称"黄粉".它缺少赖氨酸、色氨酸等人体必需氨基酸,生物学价值低,严重影响了其在食品工业中的应用.而将玉米蛋白粉经过一定得加工就可以得到玉米活性肽.玉米活性肽是比蛋白质简单、分子量小,由氨基酸通过肽键相连的一类化合物.虽然玉米活性肽的肽链较短,但易于被人体消化吸收,而且能改善身体素质等诸多保健功能.制备玉米蛋白肽的方法主要有酶法、微生物法、化学合成法.其中酶法已经得到广泛的应用,但产物具有较大的苦味;微生物法不但可以利用微生物产生的胞外酶有效地分解蛋白质,还可以通过自身的分解合成代谢去除寡肽两端的苦味;而合成法不适合工业化生产.因此,目前最佳生产玉米蛋白肽是微生物法.通过对玉米蛋白粉进行水解,可以获得降压肽、抗疲劳肽、抗氧化肽等,高F值寡肽. 相似文献
9.
10.
小麦蛋白Alcalase水解物免疫活性肽的研究 总被引:8,自引:1,他引:8
实验研究了以Alcalase碱性蛋白酶水解小麦分离蛋白制备小麦肽的工艺,分析了温度、pH值、底物浓度、酶添加量和时间对酶水解的影响。通过正交实验得到了Alcalase水解小麦蛋白的最佳工艺,工艺参数为酶用量22.5μL/g,pH值9.5,温度60℃,底物浓度4%,反应时间100min,水解度18.63%。所得小麦肽混合物用离子交换树脂依据电荷进行初步分离获得4个组分。经过小鼠体外脾淋巴细胞增殖反应(MTT法)鉴定其免疫活性,结果表明pH8和pH10洗脱得到的组分对小鼠脾细胞增殖具有较强的刺激作用,组分中可能含有对小鼠体外淋巴细胞增殖具有刺激作用的活性肽。 相似文献
11.
12.
13.
14.
15.
Corn gluten meal was hydrolyzed by alkaline protease and Flavourzyme to obtain the antioxidant peptides. The antioxidant activities of the hydrolysates or peptides were evaluated by free radical scavenging capacity (1,1-diphenyl-2-picrylhydrazyl/2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt/hydroxyl radical/superoxide radical anion), metal ion (Fe2+/Cu2+) chelating activity and lipid peroxidation inhibitory capacity. The hydrolysates were separated by ultrafiltration, and those with molecular weight <10 kDa exhibited highest antioxidant activity in all relevant assays. The hydrolysates were subsequently purified by gel filtration chromatography, and fraction F3 showed the highest antioxidant activity. Three peptides were identified from fraction F3 using LC–ESI–Q–TOF MS/MS as Leu-Pro-Phe (375.46 Da), Leu-Leu-Pro-Phe (488.64 Da) and Phe-Leu-Pro-Phe (522.64 Da). These peptides exhibited good free radical scavenging activity and lipid peroxidation inhibitory effect. Thus, corn gluten meal may be used as a potential source of antioxidant peptides for food and nutraceutical applications. 相似文献
16.
Hyung Joo Suh Song Hwan Bae Dong Ouk Noh 《Journal of the science of food and agriculture》2000,80(5):614-618
Corn gluten hydrolysates were treated with active carbons to remove the bitterness. After treatment with active carbons, the ACE (angiotensin‐converting enzyme) inhibitory activity decreased in the order of active clay, Junsei (fine and granule) and Koent CPG (67.2, 65.4 and 64.9% respectively) in TP 10K. In addition, that of PP 10K treated with Junsei (fine and granule) and active clay decreased to 75.6, 72.2 and 74.7% respectively. In PP 10K, Koent PWA and active clay effectively reduced the bitterness. PP 10K treated with Koent PWA and active clay was reported to taste as trace of bitterness to slightly bitter. TP 10K treated with Koent RC and CPG tasted as trace of bitterness to slightly bitter, and that treated with Koent PWA and Junsei (granule) tasted as slightly bitter to bitter. After treatment with active carbons, the amounts of T‐N (total nitrogen) in PP 10K and TP 10K reduced from 148 and 135 mg to 136–42 and 134–108 mg respectively. Before treatment with active carbons, PP 10K and TP 10K consisted of peptides with APLs (average peptide lengths) 14.4 and 12.8 respectively. After Junsei (fine) treatment, the APLs of PP 10K and TP 10K were 16.9 and 16.2 respectively. After treatment with the other active carbons, the APLs of PP 10K and TP 10K were 14.4–2.78 and 14.4–3.5 respectively. Before treatment with active carbons, the surface hydrophobicities of PP 10K and TP 10K were 38 and 29.9 respectively. However, after treatment with active carbons, the surface hydrophobicities of PP 10K and TP 10K were reduced, except for PP 10K treated with active clay. In PP 10K, treatments with Koent PWA and active clay markedly reduced the surface hydrophobicity from 38 to 13.3 and 14.2 respectively. The surface hydrophobicity of TP 10K treated with Koent RC and CPG was markedly reduced from 29.9 to 9.9 and 11.1 respectively. © 2000 Society of Chemical Industry 相似文献
17.
该文阐述玉米黄粉中各蛋白组分性质及提取方法,指出超声波乙醇法提取醇溶蛋白和超声波辅助碱法提取谷蛋白是目前较有推广应用价值提取法;且玉米黄粉中各蛋白组分提取及应用要根据其性质确定。 相似文献
18.
《食品与发酵工业》2019,(19):52-57
以玉米蛋白和大豆蛋白为原料,按m(玉米蛋白)∶m(大豆蛋白)=7∶3复配后,研究其水解产物的抗氧化活性。首先用米曲霉(Aspergillus oryzae)种曲和碱性蛋白酶(alcalase)协同水解玉米-大豆蛋白复配物,制备玉米-大豆蛋白水解物(CSPHs);然后通过测定CSPHs的DPPH自由基清除率和金属离子(Fe~(2+))螯合能力,分析其体外抗氧化活性;再建立双氧水(H_2O_2)氧化应激模型,探讨CSPH的细胞抗氧化活性。结果表明,CSPHs在体外具有较强的抗氧化活性,并且在加入质量分数为10%的米曲霉种曲与加入质量分数0. 8%的碱性蛋白酶情况下,协同水解后的产物CSPH4具有良好的细胞抗氧化活性。此时的水解度、可溶性蛋白含量分别为46. 10%和(73. 04±1. 68) mg/mL,·DPPH清除率和亚铁离子螯合能力分别为(41. 26±0. 69)%和(50. 23±3. 15)%。CSPH4可降低H_2O_2诱导的氧化应激人结肠癌细胞(Caco-2)细胞内活性氧(reactive oxygen species,ROS)的含量。米曲霉种曲和Alcalase协同水解复配物得到的产物具有良好的抗氧化活性,具有运用到食品工业中作为抗氧化剂的潜力。 相似文献
19.
20.
酶解100kg玉米蛋白粉制备玉米功能短肽的中试研究 总被引:9,自引:0,他引:9
酶解玉米蛋白对生产高营养且易于吸收、高附加值的具有生物学功能特性的生物产品具有重要意义。本文在多次小试实验的基础上,利用酶工程技术对100kg玉米蛋白粉水解制备玉米短肽进行了中试研究,为推动玉米蛋白资源的深加工和产业化进程提供依据。 相似文献