Protein Characteristics that Affect the Quality of Vital Wheat Gluten to be Used in Baking: A Review

The use of vital wheat gluten in the baking industry and wheat flour mills aims to improve the rheological characteristics of flour considered unsuitable to obtain products such as sliced bread, French bread, high‐fiber breads, and other products that require strong flours. To improve characteristics such as flour strength, dough mixing tolerance, and bread volume, vital wheat gluten is added to flour at levels that can vary from 2% to 10% (flour basis), with 5% being a commonly used dosage. However, the vital wheat gluten commercialized in the market has few quality specifications, especially related to the characteristics of the proteins that constitute it and are responsible for the formation of the viscoelastic gluten network. Information on protein quality is important, because variations are observed in the technological quality of vital wheat gluten obtained from different sources, which could be associated to damage caused to proteins during the obtainment process. Several tests, either physical–chemical analyses, or rheological tests, are carried out to establish gluten quality; however, they are sometimes time‐consuming and costly. Although these tests give good answers to specify gluten quality, flour mills, and the baking industries require fast and simple tests to evaluate the uses and/or dosage of vital gluten addition to wheat flour. This review covers the concepts, uses, obtainment processes, and quality analysis of vital wheat gluten, as well as simple tests to help identify details about protein quality of commercial vital wheat gluten.     

谷朊粉对高含量荞麦面团的影响及其作用机理

本文全面研究了不同添加量谷朊粉(5%、10%和15%)对高含量(50%小麦粉替代率)荞麦面团流变学特性及馒头品质的影响,并以添加10%谷朊粉的混合面团和参照组(纯小麦粉)面团为基础,通过扫描电镜,红外光谱及分析面团中的化学作用力等分析手段,进一步探讨了谷朊粉对改善混合面团流变学性质及馒头品质方面的作用机理。结果表明,添加10%的谷朊粉能够增加荞麦-小麦混合面团中二硫键的含量,改变面团中的化学作用力(离子键、氢键含量和疏水作用力),进而改变面团中面筋蛋白的构象,改善面团的微观结构,从而改善混合粉的糊化特性及面团的粉质和拉伸特性,提高馒头的比容和弹性,改善馒头芯的孔隙结构,但仍达不到参照组馒头的品质,表明添加高含量荞麦全粉不仅仅是稀释面筋蛋白。     

Fatty acids composition and rheology properties of wheat and wheat and white or brown rice flour mixture

Manufacturing of bread from rice flour only presents technological difficulty because the rice is gluten-free and gluten is the most important structure forming protein. By using wheat and rice flour mixture, this problem can be avoided, and end product is enriched by rice-oil constituents. In this paper fatty acids composition, with an emphasis on total saturated, and total unsaturated fatty acids, rheological and baking properties of wheat–rice flour mixture (70:30 w/w) were investigated. The results show that wheat–rice flour mixture has better fatty acids composition with higher content of stearic, arachidic, lignoceric, oleic, and phthalic acids compared to wheat flour. Also, wheat flour did not include myristic, arachidic, lignoceric and linolenic acids, so rice flour addition made fatty acids profile richer as number of constituents is higher, nine instead six. The content of total unsaturated fatty acids content was higher in wheat–brown rice flour mixture than in wheat flour and in wheat–white rice flour mixture. When rice flour was added to wheat flour the rheological properties were changed: flour mixture had less water absorption, less degree of softening, longer development time, higher gelatinization temperature, but better stability and finally, better quality number and group than wheat flour. So, the wheat and rice flour mixture can be considered as a good quality flour and can be used for making good quality wheat-rice bread and cake.     

谷朊粉对面包基础粉品质特性的影响

探讨不同添加量的谷朊粉对面包基础粉蛋白质的品质、流变特性及面包烘焙品质的影响。结果表明:谷朊粉可以明显增加面包基础粉的湿面筋含量、沉淀值和蛋白质含量,强化其流变学特性,有效改善面包烘焙品质,增大面包比容和高径比并改善其弹性和硬度。当谷朊粉添加量为10%时,面包烘焙品质最好。     

Combined effects of wheat gluten and carboxymethylcellulose on dough rheological behaviours and gluten network of potato–wheat flour-based bread

Incorporating high level of potato flour into wheat flour enhances nutritional values of bread but induces a series of problems that lead to the decline of the bread quality. To overcome the barrier, wheat gluten and carboxymethylcellulose (CMC) were added into potato–wheat composite flour to improve dough machinability and bread quality. The rheological properties, thermo-mechanical properties and microstructures of dough were investigated. The results showed that the interaction between gluten and CMC mitigated the discontinuity of gluten matrix and gluten protein aggregation caused by the addition of potato flour, which yielded a more branched and compact gluten network. The compact three-dimensional viscoelastic structure induced improvements of gas retention capacity and dough stability, making it mimic the machinability properties of wheat flour dough. Bread qualities were apparently improved with the combined use of 4% gluten and 6% CMC, of which specific volume increased by 42.86%, and simultaneously, hardness reduced by 75.93%.     

Dough rheology and baking performance of wheat flour–lupin protein isolate blends

The impact of addition of two lupin protein isolates (LPI), enriched either in proteins belonging to globulin (LPI G) or to albumin (LPI A) fraction, on wheat flour dough and bread characteristics was investigated. LPI addition increased the dough development time and stability plus the resistance to deformation and the extensibility of the dough. The presence of LPI proteins in dough affected bread quality in terms of volume, internal structure and texture, while extra gluten addition to the blends to compensate for wheat gluten dilution, resulting from LPI addition, led to an improvement of bread quality characteristics. Generally, the incorporation of LP isolates to wheat flour delayed bread firming. The results obtained are discussed in terms of a possible action of LPI particles as a filler of the gluten network and partly in terms of possible interactions that take place between the gluten protein constituents and those of lupin.     

青麦粉添加对馒头面团及面筋蛋白结构的影响

以馒头专用粉及青麦粉制作青麦馒头,利用激光共聚焦显微镜、黏度仪、红外光谱仪等分析仪器进行测定,探究不同青麦粉添加量(0%、5%、10%、15%、20%)对面团粉质特性、糊化特性、微观结构及面筋蛋白二级结构的影响。结果表明,随着青麦粉添加量的增加,混合粉湿面筋含量、面筋指数减少;糊化温度、峰值黏度降低,面团糊化时间缩短,面团稳定时间从6.18 min降至4.92 min、粉质质量指数减小了18,面筋强度变弱,承受力变差;面团拉伸面积、延伸度和最大拉伸阻力在135 min时显著减小;面筋网络结构逐渐出现孔洞,分布不均匀;面团面筋蛋白各吸收峰都发生了偏移,β-折叠和α-螺旋结构含量增加;综上所述,青麦粉的加入改变了面团特性和面筋蛋白结构,这些变化可能是导致青麦馒头品质下降的主要原因。     

板栗淀粉、蛋白质及全粉的特性及在面包中应用研究

基于淀粉和蛋白质对面包品质至关重要,分析板栗粉、板栗淀粉及板栗蛋白的特性,并探索对面包品质的影响。板栗粉中淀粉（52.87%）、蛋白质（6.58%）均低于高筋小麦粉。与小麦淀粉相比,板栗淀粉更易溶胀、糊化,但热稳定性较差。与小麦蛋白相比,板栗蛋白的α-螺旋含量(10.6%)较低,无规卷曲（34.4%）较高。这些特性正好反映了,随着板栗粉的添加量提高,板栗粉-小麦粉混合粉粉质品质和面包比容均呈下降趋势。当板栗粉添加量为10%时,面包体积最大、气孔细密、评分最佳。     

Effect of Amino Acids and Peptides on Mixing and Frozen Dough Properties of Wheat Flour

Free amino acids, peptides, and vital wheat gluten were investigated to determine their effect on the mixing and frozen dough baking properties of wheat flour. Addition of 1% cysteine and aspartic acid decreased and glutamic acid, histidine, arginine, and lysine increased the mixing tolerance of flour. Cystine, methionine, tryptophan, and phenylalanine increased but isoleucine, histidine, glycine, arginine, glutamic acid, aspartic acid, and lysine decreased loaf volume of nonfrozen dough breads. However cystine, methionine, tryptophan, and phenylalanine did not increase loaf volume of bread prepared from frozen dough. Vital wheat gluten increased mixing tolerance and bread loaf volume only for the nonfrozen dough. However, wheat gluten hydrolysate, corn, and bonito peptides decreased mixing tolerance after optimum mixing time and were effective in increasing loaf volume for both frozen and nonfrozen dough. As the amount of corn and bonito peptide increased, specific loaf volumes also increased. Addition of 2.5% corn peptide was most effective in increasing loaf volume of frozen dough bread. Crust browning and crumb stickiness increased, whereas crumb softness decreased with addition of peptides. Addition of less than 1% peptide did not adversely affect the aftertaste and off‐flavor of bread. These results suggest that addition of peptides are effective for improving the baking quality of frozen dough, whereas amino acids and gluten have no effect.     

豫西南小麦品种馒头制作适宜性研究

馒头是我国居民的传统主食之一。随着馒头机械化生产和市场化销售比例增加,馒头产业得到较快发展。在工业化馒头制作条件下,小麦品种特性及其面粉的馒头制作适宜性,尤其是面粉质量的稳定性,显著影响馒头的产品质量、消费体验及工业化生产的经济效益。选用豫西南地区生产上种植面积大、代表性较强的小麦品种为原料,分析其小麦粉品种的理化特性,研究馒头制作的适宜性及其小麦粉品种质量和馒头特性之间的关系。结果表明,豫西南小麦粉湿面筋含量较高,但面筋指数还有待改进;优质小麦占比有待提高;小麦粉色泽红绿值（a*值）、黄蓝值（b*值）、面筋指数、粉质参数的面团软化度、拉伸参数的拉伸面积及吹泡参数的面团延展性,是评价馒头粉质量的重要参考指标。小麦品种郑麦119、平麦998、陕道198、郑麦1342、郑麦1860比较适合制作馒头。     

Nutritional,Health, and Technological Functionality of Lupin Flour Addition to Bread and Other Baked Products: Benefits and Challenges

Lupin is an undervalued legume despite its high protein and dietary fiber content and potential health benefits. This review focuses on the nutritional value, health benefits, and technological effects of incorporating lupin flour into wheat-based bread. Results of clinical studies suggest that consuming lupin compared to wheat bread and other baked products reduce chronic disease risk markers; possibly due to increased protein and dietary fiber and bioactive compounds. However, lupin protein allergy has also been recorded. Bread quality has been improved when 10% lupin flour is substituted for refined wheat flour; possibly due to lupin-wheat protein cross-linking assisting bread volume and the high water-binding capacity (WBC) of lupin fiber delaying staling. Above 10% substitution appears to reduce bread quality due to lupin proteins low elasticity and the high WBC of its dietary fiber interrupting gluten network development. Gaps in understanding of the role of lupin flour in bread quality include the optimal formulation and processing conditions to maximize lupin incorporation, role of protein cross-linking, antistaling functionality, and bioactivity of its γ-conglutin protein.     

强筋小麦磨制面包粉工艺研究

为了研究中国育成并大面积推广强筋小麦品种的面包粉制粉工艺,以中国第一批确定的强筋面包小麦品种陕优225为主要原料,以国产4皮3芯中型加工设备进行工艺研究,同时也进行了以陕优225小麦为主原料的配麦试验。结果表明,面粉蛋白质品质,即蛋白质含量、湿面筋含量均以皮磨高于芯磨,皮磨由1B到4B逐渐升高,芯磨则由1M到3M逐渐降低;面团流变学品质除吸水率外,形成时间、稳定时间、拉伸性均与蛋白质品质规律基本一致,所不同的是面团流变学品质以3B为最高;面包烘焙品质面包体积和面包总评分其规律性与面团流变学品质相一致,皮磨以3B皮最好,以1B最差,芯磨以1M最好,3M最差。配麦试验结果显示,以陕优225与另一形成时间长的强力面包小麦以85:15配麦,取2B、3B、4B、1M、2M、3M前路粉,依次配置筛目目数为100、120、130、100、120目和130目,面粉品质明显改善,面包烘焙评分提高5.0～8.8分。     

Fortification of wheat flour with black soldier fly prepupae. Evaluation of technological and nutritional parameters of the intermediate doughs and final baked products

Bread wheat flour presents specific nutrient deficiencies, such as some essential amino acids and this drawback could be tackled by adding ingredients that contain them in high amounts. Therefore, this study aims at developing new types of flours as well as at analysing enriched flours and baked derived products, which combine the compositional and sensory characteristics of wheat bread with some peculiar nutritional properties of black soldier fly prepupae (Hermetia illucens). These composite flours were obtained by mixing “Italian type 1” semi-whole wheat flour (W = 300) with flour obtained from prepupae of black soldier fly (20 g and 40 g/1 kg composite flour). Chemical, physical, and rheological analyses were carried out for each of the flour mixtures. Moisture, ash, gluten, total protein content, falling number, strength, tenacity, extensibility, amino acid profile of doughs and breads were analysed as well. An increase in the content of essential amino acids in doughs and baked products was obtained and also led to an improvement in bread texture.     

乳酸菌发酵剂对杂粮面团及馒头品质的影响

为研究混合乳酸菌发酵对杂粮面团及馒头品质的影响,将黄豌豆粉、莜麦粉、谷朊粉及燕麦β-葡聚糖（oatmeal β-glucan,OBG）复配成杂粮粉基质,利用直投式混合乳酸菌发酵剂发酵面团制作馒头。探究乳酸菌在面团中的酸化能力,分析杂粮面团中OBG、抗性淀粉（resistant starch,RS）和游离氨基酸含量的变化以及面团的微观结构变化,并对发酵前后的杂粮馒头和小麦粉馒头进行品质及营养特性比较。结果表明,在发酵杂粮面团中乳酸菌酸化能力强,发酵24 h后乳酸和乙酸质量浓度分别达到24.85、8.98 mg/mL。与未发酵的杂粮面团相比,乳酸菌发酵杂粮面团中OBG含量下降32.56%,RS含量上升32.88%,游离氨基酸总量提升至1.46倍,氨基酸组成模式更优,部分蛋白质和纤维素发生降解,面筋网络结构更加紧密且连续。在营养方面,乳酸菌发酵前后的杂粮馒头膳食纤维质量分数均高于6%,蛋白质质量分数分别为40.35%和38.38%,属于高纤维高蛋白食品,显著优于小麦粉馒头。乳酸菌发酵杂粮馒头具有更高的体外蛋白消化率和比容,且乳酸菌发酵杂粮馒头感官评价总评分显著高于未发酵杂粮馒头,整体可接受度...     

Effects of barley flour and barley protein isolate on chemical, functional, nutritional and biological properties of Pita bread

This study was carried out to investigate the effects of fortification of wheat flour with barley flour (BF) and barley protein isolate (BPI) at three levels; 5, 10 and 15% levels on the chemical composition, nutritional evaluation and biological properties of pita bread. Proteins fractions such as globulin, prolamin, glutelin-1 and glutelin-2 as well as protein isolates were extracted from barley flour and evaluated for protein yield, chemical composition and nutritional quality. Highest yield and essential amino acids contents were obtained in barley protein isolate. SDS-PAGE gels electrophoresis indicated that fortified wheat flour with BPI and BF consists of proteins coming from wheat flour and barley proteins. The contents of essential limiting amino acids in bread were increased from 1.38 to 3.10 g/100 g for lysine and from 0.86 to 1.73 g/100 g for methionine as the ratio of fortification with BF and BPI increased from 0 to 15%. The highest content of total phenolics, antioxidant activity, and inhibitory activity for both angiotensin converting enzyme (ACE) and α-amylase were found in fortified bread with BPI at 15%. Results indicated that bread made from fortification of wheat flour with BF and BPI at 15% showed superior chemical, physico-chemical, nutritional and biological properties.     

紫苏面包粉的制备及其粉质特性分析

以面包专用粉为主料,添加紫苏油粕粉制备具有紫苏风味的紫苏面包粉,并添加适量的谷朊粉改善其粉质特性,采用粉质仪检测,以吸水量、形成时间、稳定时间和质量指数为考核指标,研究紫苏油粕粉和谷朊粉添加量对紫苏面包粉品质的影响。结果表明,紫苏油粕粉添加量10%,谷朊粉添加7%时,紫苏面包粉粉质最优:吸水量60.9%,形成时间10 min20s,稳定时间12min 36s,质量指数140。     

玉米粉配比对玉米-小麦混合粉及其馒头品质的影响

采用不同的配比将玉米粉与小麦粉搭配,通过测定玉米-小麦混合粉指标和馒头指标,以研究玉米粉对混合粉及其馒头品质的影响。结果表明:随着玉米粉比例的增加,混合粉的吸水率、稳定时间、粉质指数呈逐渐减小的趋势;最大抗延伸性、拉伸面积、沉降值、湿面筋、面筋指数也逐渐降低;峰值黏度、稀懈值、回升值、降落数值等都有所下降,而糊化温度和弱化度有所上升。玉米粉的使用在一定程度上影响了面团的流变学性能,降低了面筋的品质,但是在比例不超过15%时影响并不十分明显,辅以一定的改良技术,不但可以生产出食用品质优良的馒头,而且可以改善馒头的营养特性。     

Characterization of the Bread Made with Durum Wheat Semolina Rendered Gluten Free by Sourdough Biotechnology in Comparison with Commercial Gluten‐Free Products

Durum wheat semolina was fermented with sourdough lactic acid bacteria and fungal proteases aiming at a complete gluten hydrolysis. The gluten‐free (GF) semolina, added with naturally GF ingredients and structuring agents, was used to produce bread (rendered GF bread; rGFB) at industrial level. An integrated approach including the characterization of the main chemical, nutritional, structural, and sensory features was used to compare rGFB to a gluten‐containing bread and to 5 commercial naturally GF breads. High‐performance liquid chromatography was used for free amino acids (FAAs), organic acids, and ethanol analysis. A methanolic extract was used for determining total phenols and antioxidant activity. The bread characterization also included the analysis of dietary fibers, mycotoxins, vitamins, and heavy metals. Beyond chemical analysis, nutritional profile was evaluated considering the in vitro protein digestibility and the predicted glycemic index, while the instrumental texture profile analysis was performed to investigate the structure and the physical/mechanical properties of the baked goods. Beyond the huge potential of market expansion, the main advantages of durum wheat semolina rendered GF can be resumed in the high availability of FAAs, the high protein digestibility, the low starch hydrolysis index, and the better technological properties of bread compared to the commercial GF products currently present on the market. Vitamins, minerals, and dietary fiber profiles are comparable to those of gluten‐containing wheat bread. Also the sensory profile, determined by a panel test, can be considered the most similar to those of conventional baked goods, showing all the sourdough bread classic attributes.     

Effect of Rice, Corn and Soy Flour Addition on Characteristics of Bread Produced from Different Wheat Cultivars

Preparation and consumption of bread enriched with flours that contain appreciable amounts of protein, lysine, dietary fiber, and minerals will provide a healthy alternative to consumers and also a lowering of bread making cost in countries where wheat is not a major domestic crop. Addition of rice, corn, and soy flour to bread and durum wheat flours at 10, 20, 30, 40, 50% levels was carried out to examine the effects on the baking (specific volume, color, firmness) and sensory characteristics of bread. Dough rheological properties were also studied using Brabender Farinograph and Extensograph. Results of the present study suggest that incorporation of rice, corn, and soy in bread wheat flour up to a level of 10% (flour basis) and in durum wheat flour up to 20% produces bread without any negative effect in quality attributes such as color, hardness, and flavor and reasonable acceptance offering promising nutritious and healthy alternative to consumers. Increasing levels of substitution (30 and 50%) resulted in decreasing dough strength, extensibility, and loaf volume, due to the replacement of gluten by the added protein. Overall acceptability scores of these breads were found to be very low. The durum flour can be substituted with nongluten flours up to 10% more than the bread wheat flour because of its stronger gluten matrix and better dough rheological characteristics.     

紫苜蓿在面包焙烤和面条烹煮中的应用研究

从紫苜蓿叶中提取出的苜蓿叶蛋白含57.9%蛋白质和12.7%膳食纤维,是一种良好的食用蛋白新资源。苜蓿叶蛋白的氨基酸配比较合理,营养价值较好,但工艺特性较差。对面粉的添加量超过3%～4%时,会对面包焙烤品质、面条烹煮品质造成明显不良影响,因此通常的添加范围不超过3%～4%。当添加量增大时,需同时添加小麦活性面筋粉等品质改良剂。以苜蓿叶渣为原料经先进工艺制得的膳食纤维粉,含10.7%水溶性纤维、17.64%半纤维素、36.22%纤维素和9.24%木质素,膨胀力和持水力分别为9mL/g和800%,是一种良好的食品新资源。