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采用ELISA技术检测转Bt基因水稻中Bt蛋白的含量,判断水稻样品中是否含有转基因成分。利用研磨、酶标、孵育等技术对样品进行前处理。采用阳性质控物浓度等倍稀释方法,建立标准曲线,相关系数为0.997 4。用一系列不同转基因含量的标准基体材料,分析方法的最低检测限,灵敏度达0.1%。通过对我国进入生产性试验的转Bt基因水稻品系TT51-1和科丰6号的测试,表明该方法与普通PCR方法真实性和灵敏度一致,可以广泛应用于转Bt基因水稻及其粗加工产品的转基因成分检测。为转基因生物安全监管和安全性评价提供技术支撑。 相似文献
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综述了近几年来出现的免疫检测技术的新方法,简要介绍了它们的原理、特点及其应用,并做出相应的比较。免疫检测技术是基于抗体抗原反应的原理对待测物进行定量定性分析的检测方法,具有特异性强、灵敏度高、简便等优点,是现代生命科学的重要研究手段,在生物分析检测领域有着广泛的应用前景。此外,展望了免疫检测技术的发展方向,以期为有关的科研工作者提供参考。 相似文献
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花生是一年生草本植物,起源于南美洲热带、亚热带地区,是世界上主要的食用植物油料作物之一,在全国大部分地区都有种植。作为花生榨油之后的主要副产物,花生饼粕中约含有50%的蛋白质,丰富的花生资源为花生蛋白的研究与开发利用提供了充足的原料,由此也有力地推动了花生蛋白产品的迅速发展。花生蛋白不仅所含氨基酸种类比较齐全,而且所含人体必需氨基酸的比例较高,是植物蛋白中为数不多能替代动物蛋白的理想营养佳品,通过蛋白质改性技术可以修饰蛋白质的功能特性,提高其加工性能,拓宽花生蛋白在各领域中的应用范围。本文从物理改性、化学改性、酶法改性3个方面探讨花生蛋白改性技术对其功能特性所产生的影响,物理方法主要包括超高压均质、热处理、超声处理、低温等离子体、臭氧、反胶束和冻融循环等;化学方法包括糖基化、酰化、磷酸化、pH偏移处理和多酚化合物处理等;生物方法主要包括酶法水解和酶法交联处理两种。此外,本文总结了不同改性方法的作用机制及其对花生蛋白性质的影响,同时展望了花生蛋白改性技术的应用及发展趋势,旨在为花生蛋白的开发利用和未来发展奠定基础。 相似文献
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作者主要介绍了检测拟除虫菊酯类农药及其代谢物的酶联免疫法和荧光免疫法。通过应用新型纳米材料(如磁纳米粒子,镧系发光纳米材料)以及噬菌体展示技术提高免疫检测的灵敏度,并对拟除虫菊酯类农药及其代谢物免疫检测的发展方向进行了展望。 相似文献
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目的:建立酶标抗原的直接竞争酶联免疫吸附法(dcELISA)检测食品中虾过敏蛋白,为食品过敏诊断试剂的开发和应用提供理论基础。方法:提取虾主要过敏蛋白,免疫小鼠制备抗虾过敏蛋白多克隆抗体,辣根过氧化物酶(HRP)标记抗原,建立酶标抗原的dcELISA检测虾过敏蛋白。结果:所建立的dcELISA法最低检测限为3.94ng/mL,标准曲线在0.12~128.86ng/mL范围内线性良好,批内和批间变异系数分别为6.16%和2.73%,回收率为82%~98%。结论:该方法具有良好的特异性、敏感性和稳定性,为进一步研制检测虾过敏蛋白的ELISA试剂盒提供有效的方法。 相似文献
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Yong Tang Lu Yan Jun-jian Xiang Wu-zhou Wang Hong-yu Yang 《European Food Research and Technology》2011,232(6):963-969
A functionalized gold nanoparticles bio-barcode technology was developed to detect semicarbazide. In the assay, the hapten
CPSEM (4-[[2-(aminocarbonyl) hydrazinylidene] methyl]-benzoic acid) was first prepared and conjugated to bovine serum albumin
as the coating antigen. Subsequently, gold nanoparticles dual-labeled with anti-CPSEM monoclonal antibodies and DNA oligonucleotide
were synthesized via a one-step preparation method. Enzymatic signal was converted to DNA signal by combining polymerase chain
reaction with indirect competitive ELISA (icELISA). The limit of detection of this method could reach 8 pg mL−1 for detecting SEM, about 25 times more sensitive than conventional ELISA. The immunosorbent bio-barcode assay is a rapid
high-throughput screening tool adapted for ultra-high sensitive detection. 相似文献
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Multi-allergen screening immunoassay for the detection of protein markers of peanut and four tree nuts in chocolate 总被引:1,自引:0,他引:1
S. Ben Rejeb M. Abbott D. Davies C. Cl roux P. Delahaut 《Food Additives & Contaminants》2005,22(8):709-715
A multiresidue enzyme immunoassay was developed to check for the presence of markers of peanut, hazelnut, almond, cashew and Brazil nuts in a single run. The assay was designed under the competitive indirect format and adapted for screening purposes applied to chocolate samples. The limit of detection for this assay was below 1 µg g-1 protein for each allergenic food. In most cases, the high specificity of the antibodies used allowed the identification of each particular allergenic food with no possible confusion. This assay was proven to be useful as part of an analytical procedure involving the identification of the unknown allergenic food among peanut and other tree nuts in recalled samples before the application of a quantitative technique to determine the level of cross-contamination. 相似文献
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为建立用于快速检测样品中氯霉素残留含量的胶体金免疫层析试纸条,采用免疫竞争法,将抗氯霉素单克隆抗体一胶体金复合物包被在胶体金结合垫上,并将人工合成的氯霉素抗原包被在硝酸纤维素薄膜表面作为检测线(T线),其与待测样品中氯霉素竞争结合胶体金标记的氯霉素单克隆抗体,并能以颜色直观显示检测的定性结果。检测虾肉等组织试样时,灵敏度最低值可达到1ng/ml,只需5—10min,与类似物无交叉反应。试纸条具有较高的灵敏度及特异性,操作便捷.稳定可靠,可作为氯霉素残留现场监控的有效筛检手段。 相似文献
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A rapid and inexpensive procedure to detect polychlorinated biphenyls (PCBs) in transformer oil is needed to facilitate identification and removal of PCB contaminated transformers. Here we describe a simple two-step liquid-liquid extraction using acidic dimethyl sulfoxide in conjunction with an immunoassay for detecting PCBs in transformer oil. The process described is faster and simpler than any previous immunoassay while maintaining comparable detection limit and false negative rate. Cross reactivity data, characterizing the immunoassay response to the four Kanechlor technical mixtures of PCBs in oil, are presented. Forty-five used transformer oil samples were analyzed by gas chromatography-high-resolution mass spectrometry and were also evaluated using the immunoassay protocol developed. Results presented show zero false negatives at a 1.4 ppm nominal cutoff for the transformer oils analyzed. 相似文献
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霉菌毒素污染严重危害食品的质量安全,最终影响到人们的健康。因此,建立毒素检测方法对预防霉菌毒素污染,减少毒素中毒事件发生至关重要。综述了污染粮食的霉菌毒素的种类、危害及免疫标记技术在粮食霉菌毒素检测中的应用。 相似文献
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Christine Hupfer Johann Mayer Helmut Hotzel Konrad Sachse K.-H. Engel 《European Food Research and Technology》1999,209(5):301-304
The detection of the genetic modification in silage obtained from insect-resistant Bt maize by means of the polymerase chain
reaction (PCR) is described. The detectability of the transgene was shown to be dependent on the length of the genomic target
sequence chosen for amplication by the PCR. By amplifying a Bt-maize-specific DNA sequence of 211 bp the genetic modification
was detected up to 7 months after ensilage. The effect of maize DNA degradation in the course of the ensilage on the detectability
of target sequences was demonstrated in model experiments.
Received: 17 December 1998 相似文献
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M. Ermolli A. Prospero B. Balla M. Querci A. Mazzeo G. Van Den Eede 《Food Additives & Contaminants》2006,23(9):876-882
An innovative immunoassay, called enzyme-linked immunoabsorbant assay (ELISA) Reverse, based on a new conformation of the solid phase, was developed. The solid support was expressly designed to be immersed directly in liquid samples to detect the presence of protein targets. Its application is proposed in those cases where a large number of samples have to be screened simultaneously or when the simultaneous detection of different proteins is required. As a first application, a quantitative immunoassay for Cry1AB protein in genetically modified maize was optimized. The method was tested using genetically modified organism concentrations from 0.1 to 2.0%. The limit of detection and limit of quantitation of the method were determined as 0.0056 and 0.0168 (expressed as the percentage of genetically modified organisms content), respectively. A qualitative multiplex assay to assess the presence of two genetically modified proteins simultaneously was also established for the case of the Cry1AB and the CP4EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) present in genetically modified maize and soy, respectively. 相似文献