Nitrogen fertilizer in leucaena alley cropping. I. Maize response to nitrogen fertilizer and fate of fertilizer-15N

Field microplot experiments were conducted in the semi-arid tropics of northern Australia to evaluate the response of maize (Zea mays L.) growth to addition of N fertilizer and plant residues and to examine the fate of fertilizer¹⁵N in a leucaena (Leucaena leucocephala) alley cropping system, in which supplemental irrigation was used. Leucaena prunings, maize residues and N fertilizer were applied to alley-cropped maize grown in microplots which were installed in the alleys formed by leucaena hedgerows spaced 4.5 metres apart. The¹⁵N-labelled fertilizer was used to examine the fate of fertilizer N applied in the presence of mulched leucaena prunings and maize residues.Application of leucaena prunings increased maize yield while addition of N fertilizer in the presence of the prunings produced a further increase in maize production. There was a significant positive interaction between N fertilizer and leucaena prunings in increasing maize production. The addition of maize residues in the presence of N fertilizer and leucaena prunings decreased maize yield and N uptake and increased fertilizer¹⁵N loss from 38% to 47%. Maize recovered 24–79% of fertilizer¹⁵N in one cropping season, depending on application rate of N fertilizer and field management of plant residues. About 20–34% of fertilizer¹⁵N remained in the soil. More than 37% of fertilizer¹⁵N was apparently lost from the soil and plant system largely through denitrification when N fertilizer was applied at 40 kg N ha^–1 or more in the presence or absence of plant residues. Application of N fertilizer improved maize yield and increased the contribution of mulched leucaena prunings to crop production in the alley cropping system.     

Recovery of 15N-labelled fertilizer applied to winter wheat and perennial ryegrass crops and residual 15N recovery by succeeding wheat crops under different crop residue management practices

The recovery of ¹⁵N-labelled fertilizer applied to a winter wheat (120 kg N ha^–1) and also a perennial ryegrass (60 kg N ha^–1) crop grown for seed for 1 year in the Canterbury region of New Zealand in the 1993/94 season was studied in the field. After harvests, ryegrass and wheat residues were subjected to four different residue management practices (i.e. ploughed, rotary hoed, mulched and burned) and three subsequent wheat crops were grown, the first succeeding wheat crop sown in 1994/95 to examine the effects of different crop residue management practices on the residual ¹⁵N recovery by succeeding wheat crops. Total ¹⁵N recoveries by the winter wheat and ryegrass (seed, roots and tops) were 52% and 41%, respectively. Corresponding losses of ¹⁵N from the crop-soil systems represented by un-recovered ¹⁵N in crop and soil were 12% and 35%, respectively. These losses were attributed to leaching and denitrification. The proportions of ¹⁵N retained in the soil (0-400 mm depth) at the time of harvest of winter wheat and ryegrass were 36% and 24%, respectively. Although the soil functioned as a substantial sink for fertilizer N, the recovery of this residual fertilizer by subsequent three winter wheat crops was low (1-5%) and this was not affected by different crop residue management practices.     

Fate of Fertilizer15N applied as urea and ammonium sulphate in opium poppy (Papaver somniferum L.) grown under greenhouse conditions

Laboratory incubation and greenhouse experiments were conducted to investigate the comparative effectiveness of urea and ammonium sulphate in opium poppy (Papaver somniferum L.) using¹⁵N dilution techniques. Fertilizer treatments were control (no N), 600 mg N pot^–1 and 1200 mg N pot^–1 (12 kg oven dry soil) applied as aqueous solution of urea or ammonium sulphate. Fertilizer rates, under laboratory incubation study were similar to that under greenhouse conditions. A fertilizer¹⁵N balance sheet reveals that N recovery by plants was 28–39% with urea and 35–45% with ammonium sulphate. Total recovery of¹⁵N in soil-plant system was 77–82% in urea. The corresponding estimates for ammonium sulphate were 89–91%. Consequently the unaccounted fertilizer N was higher under urea (18–23%) as compared to that in ammonium sulphate (9–11%). The soil pH increased from 8.2 to 9.4 with urea whereas in ammonium sulphate treated soil pH decreased to 7.3 during 30 days after fertilizer application. The rate of NH₃ volatilization, measured under laboratory conditions, was higher with urea as compared to the same level of ammonium sulphate. The changes in pH of soil followed the identical trend both under laboratory and greenhouse conditions.     

The effect of plant residues on plant uptake and leaching of soil and fertilizer nitrogen in a tropical red earth soil

Two field experiments, in which differing amounts and types of plant residues were incorporated into a red earth soil, were conducted at Katherine, N.T., Australia. The aim of the work was to evaluate the effect of the residues on uptake of soil and fertilizer N by a subsequent sorghum crop, on the accumulation and leaching of nitrate, and on losses of N.Stubble of grain sorghum applied at an exceptionally high rate (~ 18 000 kg ha^–1) reduced uptake of N by sorghum by 13% and depressed the accumulation of nitrate under a crop and particularly under a fallow.Loss of fertilizer N, movement of nitrate down the profile, and uptake by the crop was studied in another experiment after application of N as¹⁵NH₄ ¹⁵NO₃ to field microplots. By four weeks after fertilizer application 14% had been lost from the soil-plant system and by crop maturity 36 per cent had been lost. The pattern of¹⁵N distribution in the profile suggested that losses below 150 cm had occurred during crop growth. The recovery of¹⁵N by the crop alone ranged from 16 to 32 per cent. There was an apparent loss of N from the crop between anthesis and maturity. Residue levels common to sorghum crops in the region (~ 2000 kg ha^–1) did not significantly affect uptake by a subsequent sorghum crop, N losses, or distribution of nitrate in the profile.     

Uptake and balance of labelled fertilizer nitrogen by potatoes

Potatoes have a shallow rooting system. This can seriously affect the efficient use of fertilizer N. During two consecutive years, 1985 and 1986, a study was conducted on a commercial field to investigate the uptake of labelled N by potatoes under the recommended N rate and existing agricultural practices. The fertilizer efficiency, fertilizer distribution within the plant and soil and the total fertilizer balance were made using¹⁵NH₄ ¹⁵NO₃ 3.63 At. %¹⁵N excess. The recovery of the applied N-fertilizer in the whole plant was 25 and 56% for 1985 and 1986, respectively. The % Ndff and % Ndfs ranged between 30–40% and 60–70% respectively in both years. An important amount of fertilizer N was left in the soil after harvest. It reached 44 and 34% in 1985 and 1986, respectively.The total balance of the applied fertilizer N showed that up to 31 and 10% of the fertilizer N was lost during 1985 and 1986, respectively. The differences between the two growing seasons were mainly related to the method and timing of fertilizer N application and to the amount of rainfall.     

Detectability of15N-depleted fertilizer N in soil and plant tissue during a corn-rye crop rotation

Use of¹⁵N-depleted fertilizer materials have been primarily limited to fertilizer recovery studies of short duration. The objective of this study was to determine if¹⁵N-depleted fertilizer N could be satisfactorily used as a tracer of residual fertilizer N in plant tissue and various soil N fractions through a corn (Zea mays L.) -winter rye (Secale cereale L.) crop rotation. Nitrogen as¹⁵N-depleted (NH₄)₂SO₄ was applied at five rates (0, 84, 168, 252, and 336 kg N ha^–1) to corn. Immediately following corn harvest a winter rye cover crop treatment was initiated. Residual fertilizer N was easily detected in the soil NO ₃ ^- -N fraction following corn harvest (140-d after application). Low levels of exchangeable NH ₄ ⁺ -N (<2.5 mg kg^–1) did not permit accurate isotope-ratio analysis. Fertilizer-derived N recovered in the soil total N fraction following corn harvest was detectable in the 0 to 30-cm depth at each N rate and in the 30 to 60 and 60 to 90-cm depths at the 336 kg ha^–1 N rate. Atom %¹⁵N concentrations in the nonexchangeable NH ₄ ⁺ -N fraction did not differ from the control at each N rate. Nitrogen recovery by the winter rye cover crop reduced residual soil NO ₃ ^- -N levels below the 10 kg ha^–1 level needed for accurate isotope-ratio analysis. Atom %¹⁵N concentrations in the soil total N fraction (approximately one yr after application) were indistinguishable from the control plots below the 168, 252, and 336 kg ha^–1 N rate at the 0 to 30, 30 to 60, and 60 to 90-cm depths, respectively. Recovery of residual fertilizer N by the winter rye cover crop was verified by measuring significant decreases in atom %¹⁵N concentrations in rye tissue with increasing N rates. The greatest limitation to the use of¹⁵N-depleted fertilizer N as a tracer of residual fertilizer N in a corn-rye crop rotation appears to be its detectibility from native soil N in the total N pool.Research partially supported by grants from the National Fertilizer and Environmental Research Center/TVA and the Virginia Division of Soil and Water Conservation.     

The effect of fertilizer placement on nitrogen uptake and yield of wheat and maize in Chinese loess soils

Field trials were carried out to study the fate of¹⁵N-labelled urea applied to summer maize and winter wheat in loess soils in Shaanxi Province, north-west China. In the maize experiment, nitrogen was applied at rates of 0 or 210 kg N ha^–1, either as a surface application, mixed uniformly with the top 0.15 m of soil, or placed in holes 0.1 m deep adjacent to each plant and then covered with soil. In the wheat experiment, nitrogen was applied at rates of 0, 75 or 150 kg N ha^–1, either to the surface, or incorporated by mixing with the top 0.15 m, or placed in a band at 0.15 m depth. Measurements were made of crop N uptake, residual fertilizer N and soil mineral N. The total above-ground dry matter yield of maize varied between 7.6 and 11.9 t ha^–1. The crop recovery of fertilizer N following point placement was 25% of that applied, which was higher than that from the surface application (18%) or incorporation by mixing (18%). The total grain yield of wheat varied between 4.3 and 4.7 t ha^–1. In the surface applications, the recovery of fertilizer-derived nitrogen (25%) was considerably lower than that from the mixing treatments and banded placements (33 and 36%). The fertilizer N application rate had a significant effect on grain and total dry matter yield, as well as on total N uptake and grain N contents. The main mechanism for loss of N appeared to be by ammonia volatilization, rather than leaching. High mineral N concentrations remained in the soil at harvest, following both crops, demonstrating a potential for significant reductions in N application rates without associated loss in yield.     

Uptake of fertilizer and soil nitrogen by ryegrass swards during spring and mid-season

Double-labelled¹⁵N ammonium nitrate was used to determine the uptake of fertilizer and soil N by ryegrass swards during spring and mid-season. The effects of water stress (40% of mean rainfall v 25 mm irrigation per 25 mm soil water deficit) and the rate of application of N in the spring (40 v 130 kg ha^–1) on the recovery of 130 kg N ha^–1 applied in mid-season were also evaluated. Apparent recovery of fertilizer N (uptake of N in the fertilized plot minus that in the control expressed as a percentage of the N applied) was 95 and 79% for fertilizer N applied in the spring at rates of 40 and 130 kg ha^–1, respectively. Actual recovery of the fertilizer N assessed from the uptake of¹⁵N was only 31 and 48%, respectively. The uptake of soil N by the fertilized swards was substantially greater than that by the control. However, the increased uptake of soil N was always less than the amount of fertilizer N retained in or lost from the soil. Broadly similar patterns for the uptake of fertilizer and soil N were observed during mid-season. Uptake of N in mid-season was highest for swards which received 40 kg N ha^–1 in the spring and suffered minimal water stress during this period. Application of 130 kg N ha^–1 in spring reduced the uptake of N in mid-season to an extent similar to that arising from water stress. Only 1.8 to 4.2 kg ha^–1 (3 to 10%) of the N residual from fertilizer applied in the spring was recovered during mid-season. Laboratory incubation studies suggested that only a small part of the increased uptake of soil N by fertilized swards could be attributed to increased mineralisation of soil N induced by addition of fertilizer. It is considered that the increased uptake of soil N is partly real but mostly apparent, the latter arising from microbially mediated exchange of inorganic¹⁵N in the soil.     

The effect of autumn applied 15N-labelled fertilizer on nitrate leaching in a cultivated soil during winter

Nitrogen (N) fertilizer applied in autumn to arable farm land raises concerns over affects on ground water quality. The contribution of autumn ¹⁵N-labelled fertilizer (50 kg N ha^-1) to nitrate leaching losses from a cultivated soil (silt loam on sandy loam; Udic Ustochrept) was measured using undisturbed monolith lysimeters (500 mm diameter, 700 mm long) during consecutive winters in Canterbury, New Zealand. The addition of ¹⁵N-labelled fertilizer at 50 kg N ha^-1 did not significantly increase nitrate leaching losses. Soil-derived-N contributed 78 and 88% (1996 and 1997, respectively) of the nitrate leached beneath fertilized lysimeters. Warmer weather and wetter soil conditions at cultivation and fertilizer application during 1997, compared with 1996, resulted in an increased release of soil-derived-N in 1997. Nitrate leaching and average nitrate concentrations were therefore 41% and 56% higher, respectively, during the winter of 1997 than the winter of 1996. However, fertilizer leaching losses were relatively consistent between years (7.8 and 8.6%). Although not statistically significant, total N leaching losses and average nitrate concentration were 24 to 30% higher below fertilized lysimeters as compared with unfertilized lysimeters, indicating a priming effect of fertilizer on soil N release. During both late winter periods, leachate nitrate concentrations from fertilized and unfertilized lysimeters exceeded World Health Organisation (WHO) limits for drinking water. Higher release of soil-derived-N in 1997 also meant WHO limits were exceeded for 6 weeks longer than in 1996. In conclusion, the application of ¹⁵N fertilizer in autumn directly contributed only a small proportion of the total amount of N leached in this cultivated soil. However, the apparent priming effect of autumn applied-N fertilizer has importance on the overall environmental impact of this production system, as the amount of N leached, and extent to which health limits were exceeded, was largely determined by the factors which controlled the release of soil-derived-N.     

Sugarcane residue management and grain legume crop effects on N dynamics,N losses and growth of sugarcane

To reduce greenhouse gas emissions farmers are being encouraged not to burn sugarcane residues. An experiment was set up in NE Thailand, where sugarcane residues of the last ratoon crop were either burned, surface mulched or incorporated and subsequently the field left fallow or planted to groundnut or soybean. The objectives of the current experiment were to evaluate the residual effects of these treatments during the following new sugarcane crop on (i) microbial and mineral N dynamics, (ii) performance of sugarcane and (iii) effectiveness of recycled legume residues compared to mineral N fertilizer on N use efficiencies, ¹⁵N recovery in the system and in soil particle size and density fractions (using ¹⁵N labelled legume residues and fertilizer). The millable cane and sugar yield were positively affected by sugarcane residue mulching and incorporation compared to burning suggesting microbial remobilization of previously immobilized N. Residual effects of legumes increased sugarcane tillering and yield (127 and 116 Mg ha⁻¹ for groundnut and soybean, respectively) compared to the fallow treatment without N fertilizer (112 Mg ha⁻¹). Soybean residues of higher C:N ratio (33:1) and lignin content (13%) compared to groundnut residues (21:1 C:N, 5% lignin) decomposed slower and improved N synchrony with cane N demand. This led to a better conservation of residue N in the system with proportionally less ¹⁵N losses (15–17%) compared to the large losses from groundnut residues (50–57%) or from mineral N fertilizer (50–63%). ¹⁵N recoveries in soil were larger from residues (41–80%) than from fertilizer (30%) at final harvest. Recycled legume residues were able to substitute basal fertilizer N application but not topdressing after 6 months.     

Effect of climate on the recovery in crop and soil of15N-labelled fertilizer applied to wheat

Data was assembled from experiments on the fate of¹⁵N-labelled fertilizer applied to wheat (Triticum spp.) grown in different parts of the world. These data were then ranked according to the annual precipitation-evaporation quotient for each experimental location calculated from the average long-term values of precipitation and potential evaporation. Percentage recovery of¹⁵N fertilizer in crop and soil varied with location in accordance with the precipitation-evaporation quotient. In humid environments more¹⁵N fertilizer was recovered in the crop than in the soil, while in dry environments more¹⁵N fertilizer was recovered in the soil than in the crop. Irrespective of climatic differences between locations 20% (on average) of the¹⁵N fertilizer applied to wheat crops was unaccounted for at harvest. Most of the¹⁵N fertilizer remaining in the soil was found in the 0–30 cm layer. The most likely explanation of these differences is that wheat grown in dry environments has a greater root:shoot ratio than wheat grown in humid environments and, further, that the residue of dryland crops have higher C/N ratios. Both factors could contribute to the greater recovery of¹⁵N fertilizer in the soil in dry environments than in humid ones.     

Influence of delaying flood and preflood nitrogen application on dry-seeded rice

In the southern U.S. rice belt it is recommended that rice (Oryza sativa L.) grown in the dry-seeded, delayed flood cultural system have the preflood N fertilizer applied and the field flooded at the fourth to fifth leaf stage of plant development. The objective of this field study was to determine if delaying the flood and preflood N application past the fifth leaf stage was detrimental to rice total N and fertilizer¹⁵N uptake, total dry matter, and grain yield. This study was conducted on a Crowley silt loam (Typic Albaqualfs) and a Perry clay (Vertic Haplaquepts). The preflood N fertilizer and flood were delayed 0, 7, 14, or 21 d past the fourth to fifth leaf stage, after which time a permanent flood was established and maintained until maturity. All treatments received 20.5 g N m^–2 as¹⁵N-labeled urea in three topdress applications. All plant and soil samples were taken at maturity. Harvest index increased as the preflood N and flood were delayed past the 4 to 5 leaf stage. Total N in the grain + straw either decreased or showed a decreasing trend as the N and flood were delayed. Similarly, uptake of native soil N decreased as flood was delayed. Conversely, percent recovery of fertilizer N in the rice plant and the plant-soil system increased as the preflood N and flood were delayed. Rice grain yield was not significantly affected by delaying the preflood N and flood up to 21 d.Received....... . Published with permission of the Director of the Arkansas Agric. Exp. Stn. Project ARK01386. Supported in part by the Tennessee Valley Authority National Fertilizer and Environmental Research Center and the Arkansas Rice Research and Promotion Board.     

Nitrogen losses from fertilizers applied to maize, wheat and rice in the North China Plain

Ammonia volatilization, denitrification loss and total nitrogen (N) loss (unaccounted-for N) have been investigated from N fertilizer applied to a calcareous sandy loam fluvo-aquic soil at Fengqiu in the North China Plain. Ammonia volatilization was measured by the micrometeorological mass balance method, denitrification by the acetylene inhibition – soil core incubation technique, and total N loss by ¹⁵N-balance technique. Ammonia loss was an important pathway of N loss from N fertilizer applied to rice (30–39% of the applied N) and maize (11–48%), but less so for wheat (1–20%). The amounts of unaccounted-for fertilizer N were in the order of rice > maize > wheat. Deep placement greatly reduced ammonia volatilization and total N loss. Temperature, wind speed, and solar radiation (particular for rice), and source of N fertilizer also affect extent and pattern of ammonia loss. Denitrification (its major gas products are N₂ and N₂O) usually was not a significant pathway of N loss from N fertilizer applied to maize and wheat. The amount of N₂O emission (N₂O is an intermediate product from both nitrification and denitrification) was comparable to denitrification loss for maize and wheat, and it was not significant in the economy of fertilizer N in agronomical terms, but it is of great concern for the environment.     

Initial and residual effects of nitrogen fertilizers on grain yield of a maize/bean intercrop grown on a Humic Nitosol and the fate and efficiency of the applied nitrogen

Initial and residual effects of nitrogen (N) fertilizers on grain yield of a maize/bean intercrop grown on a deep, well-drained Humic Nitosol (66% clay, 3% organic carbon) were evaluated. Enriched (¹⁵N) N fertilizer was used to study the fate of applied N in two seasons: using urea (banded) at 50 kg N ha^–1 in one season, and¹⁵N-enriched urea (banded), calcium ammonium nitrate (CAN, banded), and urea supergranules (USG, point placement) were applied in the other season (different field) at 100 kg N ha^–1. Nitrogen fertilizer significantly (P = 0.05) increased equivalent maize grain yield in each season of application with no significant differences between N sources, i.e., urea, CAN, and USG. Profitmaximizing rates ranged from 75 to 97 kg N ha^–1 and value: cost ratios ranged from 3.0 to 4.8. Urea gave the highest value: cost ratio in each season. Most (lowest measurement 81%) of the applied N was accounted for by analyzing the soil (to 150 cm depth) and plant material. Measurements for urea, CAN, and USG were not significantly different. The high N measurements suggest low losses of applied N fertilizer under the conditions of the study. Maize plant recovery ranged from 35 to 55%; most of this N (51–65%) was in the grain. Bean plant recovery ranged from 8 to 20%. About 34–43% of the applied N fertilizer remained in the soil, and most of it (about 70%) was within the top soil layer (0–30 cm). However, there were no significant equivalent maize grain increases in seasons following N application indicating no beneficial residual effect of the applied fertilizers.     

Residual N and P fertilizer effect and fertilizer recovery on intercropped and sole-cropped corn and bean in semiarid northeast Brazil

The effects of a single ¹⁵N and P fertilizer application (16 and 12 kg ha^–1) on intercropped and sole-cropped corn and beans was followed over three consecutive years. Grain (0.1–0.9 ton ha^–1 yr^–1) and straw productions (0.2–2.5 ton ha^–1 yr^–1) were limited by rainfall and showed small responses to fertilizer. In the first year, plant N uptake was more than twice the fertilizer amounts, while P uptake was less than half the fertilizer amounts. Plant N derived from fertilizer was low (9–19%). Sole corn took up more (34%) than beans (16%) and the combined intercrop (26%) and also had higher recovery of fertilizer in the soil than single beans (50% against 28%). The distribution of fertilizer N and P in the soil showed a similar pattern in all treatments, with a high concentration around the application spot and decreasing concentrations at greater distances and above and below this point. Total P increases in a soil volume 10 cm around the application spot corresponded to 60% of the amount applied. Fertilizer contributions to the second crop were < 3% of total plant N and represented <6% of the applied amount. Therefore, the residual fertilizer effect on production was attributable to P. The patterns of fertilizer N and P distribution in the soil remained similar but N recoveries decreased 14–18%. Despite low rainfall, low productivities and reasonable proportions of fertilizer N remaining in the soil, the residual effects of the applied fertilizer N were too low to justify a fertilizer recommendation based on economic returns on the investment.     

Impact of organic residue management on nitrogen use efficiency in an annual rice cropping sequence of lowland Central Thailand

Field experiments were conducted in Central Thailand under a rice–fallow–rice cropping sequence during consecutive dry and wet seasons of 1998 to determine the impact of residue management on fertilizer nitrogen (N) use. Treatments consisted of a combination of broadcast urea (70 kg N ha^–1) with rice straw (C/N 67) and rice hull ash (C/N 76), which were incorporated into the puddled soil 1 week before transplanting at a rate of 5 Mg ha^–1. Nitrogen-15 balance data showed that the dry season rice recovered 10 to 20% of fertilizer N at maturity. Of the applied N, 27 to 36% remained in the soil. Loss of N (unaccounted for) from the soil–plant system ranged from 47 to 54% of applied N. The availability of the residue fertilizer N to a subsequent rice crop was only less than 3% of the initial applied N. During both season fallows NO₃-N remained the dominant form of mineral-N (NO₃+NH₄) in the aerobic soil. In the dry season grain yield response to N application was significant (P=0.05). Organic material sources did not significantly change grain yield and N accumulation in rice. In terms of grain yields and N uptake at maturity, there was no significant residual effect of fertilizer N on the subsequent rice crop. The combined use of organic residues with urea did not improve N use efficiency, reduced N losses nor produced higher yields compared to urea alone. These results suggested that mechanisms such as N loss through gaseous N emissions may account for the low fertilizer N use efficiency from this rice cropping system. Splitting fertilizer N application should be considered on the fertilizer N use from the organic residue amendment.     

Response of dryland wheat to fertilizer nitrogen in relation to stored water,rainfall and residual farm yard manure

Yield response of dryland wheat to fertilizer N application in relation to components of seasonal water (available soil moisture and rainfall) and residual farm yard manure (FYM) was studied for five years (1983–84 to 1987–88) on a maize-wheat sequence on sandy loam soils in Hoshiarpur district of Punjab, India. Four rates of N viz. 0, 40, 60 and 80 kg ha^–1 in wheat were superimposed on two residual FYM treatments viz. no FYM (F₀) and 15 t ha^–1 (F₁₅) to preceding maize. FYM application to maize increased the residual NO₃-N content by 19–30 kg ha^–1 in the 180 cm soil profile. For a given moisture distribution, F₁₅ increased attainable yields. Over the years, F₁₅ increased wheat yield by 230 to 520 kg ha^–1. Response to fertilizer N was lower in FYM amended plots than in unamended plots. Available soil moisture at wheat seeding and amount and distribution of rainfall during the vegetative and the reproductive phases of crop development affected N use efficiency by wheat. Available soil moisture at seeding alone accounted for 50% variation in yield. The residual effect of FYM on wheat yield could be accounted for by considering NO₃-N in 180 cm soil profile at seeding. The NO₃-N and available soil moisture at wheat seeding along with split rainfall for two main phases of crop development and fertilizer N accounted for 96% variation in wheat yield across years and FYM treatments.     

Nitrogen use efficiency of <Superscript>15</Superscript>N-labelled sheep manure and mineral fertiliser applied to microplots in long-term organic and conventional cropping systems

Nitrogen (N) utilisation by crops has to be improved to minimize losses to the environment. We investigated N use efficiency of animal manure and mineral fertiliser and fate of fertiliser N not taken up by crops in a conventional (CONMIN) and a bio-organic (BIOORG) cropping system of a long-term field experiment over three vegetation periods (winter wheat–soybean–maize). Microplots planted with wheat received a single application of ¹⁵N-labelled slurries (either urine or faeces labelled) or mineral fertiliser. At the end of each vegetation period we tested whether higher microbial activity and larger microbial biomass in BIOORG than CONMIN soils, and lower long-term N input level in BIOORG, affected use efficiency and fate of fertiliser N not taken up by crops. Recovery of ¹⁵N in wheat was 37%, 10% and 47% from urine, faeces and mineral fertiliser, respectively, and decreased strongly in the residual years. In total 41%, 15% and 50% of ¹⁵N applied as urine, faeces and mineral fertiliser was recovered by the three crops. ¹⁵N recovered from originally applied urine, faeces and mineral fertiliser in the topsoil (0–18 cm) at the end of the third vegetation period was 19%, 25% and 20%, respectively. Of urine-, faeces- and mineral fertiliser-¹⁵N, 40%, 61% and 29%, respectively, was not recovered by the three crops and in topsoil suggesting significant transport of ¹⁵N-labelled components to deeper soil layers. CONMIN and BIOORG differed neither in fertiliser N use efficiency by crops nor in ¹⁵N recovery in soil indicating insignificant difference in the turnover and utilization of the applied manure nitrogen in the conventional and the bio-organic cropping systems.     

Nitrogen utilization by lowland rice as affected by fertilization with urea and green manure

Field studies were conducted during two consecutive wet seasons in flooded rice (Oryza sativa L.) to determine the effect of green manure on urea utilization in a rice-fallow-rice cropping sequence. Replicated plots were fertilized with 60 to 120 kg of urea N ha^–1 in three split applications (50, 25 and 25%) with or without incorporation of dhaincha (Sesbania aculeata L.) (100 kg N ha^–1). During the first crop only 31 to 44% of the urea added was used by the rice. Incorporatingin situ grown dhaincha (GM) into the soil at transplanting had little effect on urea utilization. Forty-four to 54% of the N added was not recovered in the soil, rice crop, or as nitrate leachate during the first cropping season. Incorporation of GM had no effect on fertilizer N recovery. Only about 2% of the urea N added to the first rice crop was taken up by the second rice crop and, as in the first crop, the GM had little effect on residual N, either in amount or utilization.     

1. The chemistry and biology of flooded soils in relation to the nitrogen economy in rice fields

Response of lowland rice to sources and methods of nitrogen fertilizer application were summarized for more than 100 experiments. In about 2/3 of the experiments, the yield increase per kg of fertilizer N was judged to be relatively poor with best split applications of urea. Based on frequency distribution, sulfur coated urea and urea briquets or urea supergranules deep placed more often produced satisfactory yield increases than best split urea, but even with these sources/methods the yield increases were judged to be relatively poor in about 1/2 of the experiments. There is an enormous potential to increase rice production with no further increases in inputs of fertilizer N if we could learn to match the best method/source of fertilizer with the soil-crop management complex.About 60% of the yields with no fertilizer N were in the range of 2 to 4 t/ha. Based on the average yield response to urea, this is equivalent to about 100 kg of urea N. It would appear worthwhile to study ways to improve utilization of soil nitrogen since it is already in place on the land and apparently in fairly abundant amounts in many soils.About 50 experiments with¹⁵N fertilizers were summarized. In almost all cases, the uptake of tagged fertilizer was less than the net increase in N in the above ground matter. In about 2/3 of the experiments, the addition of fertilizer N increased soil N uptake more than 20% and in 1/3 of the experiments the uptake of soil N was increased more than 40%. These results lead to much uncertainty about practical interpretation and use of¹⁵N data.