痕量甲苯咪唑的高效液相色谱法测定

建立了用ＨＰＬＣ测定痕量甲苯咪唑的分析方法，该方法成功地用于甲苯咪唑片生产设备清洗液中痕量甲苯咪唑的测定。方法准确度１００．４％，检出范围０．２５μｇ－２．５μｇ，棉签回收率７３．１６％，精密度０．２１％。     

催化光度法测定痕量铜（Ⅱ）

基于氨介质中，铜离子催化过氧化氢氧化硝蛋黄而使其褪色的反应，建立了测定痕量铜的新方法。方法灵敏，简便，选择性了。测定铜（Ⅱ）的线性范围为０￣１０μｇ／Ｌ，检出量为０．３μｇ／Ｌ。用于水及食品中痕量铜（Ⅱ）的测定。     

盐酸联氨还原亮甲酚蓝催化光度法测定痕量钼

基于钼（Ⅵ）催化盐酸联氨还原亮甲酚蓝反应，拟定了测定痕量钼的新催化光度法。在不同温度条件下，可测钼含量范围为０．０２５～０．６０μｇ／ｍｌ，方法检出限为９．６×１０－９ｇ／ｍｌ，用于测定豆类中痕量钼。     

流动注射光度法测定水中痕量镉

用苯重氮氢基苯对偶氮苯作显色剂建立了测定痕量镉的流动注射分光光度分析法。在四硼酸钠－氢氧化钠溶液作载流的缓冲介质中，当乳化剂ＯＰ存在时，笨重氮氨基苯对偶氮苯与镉形成的红色配合物的吸光度在０．０４～０．４０μｇ／ｍｌ范围内呈良好的线性关系。考察了各种实验条件，方法简单、选择性好，进样体积为１００μｌ，测样频率每小时为７６次。在室温条件下，检出限达到０．００６μｇ／ｍｌ。对０．１２８μｇ／ｍｌ镉的１１次检测，变异系数为１．５％。平均回收率为１００．３％。方法用于水中镉的测定，结果良好。     

溴氯酚蓝催化动力学光度法测定痕量铁

本文研究了在硫酸介质中，铁（Ⅲ）催化过氧化氢氧化溴氯酚蓝而使其褪色的反应，建立了一种催化动力学光度法测定痕量铁的新方法。本方法的测定范围为０～２．１μｇ／２５ｍｌ，检出限为４．１×１０－１０ｇ／ｍｌ，用于水样及人发样品中痕量铁（Ⅲ）的测定，相对标准偏差小于５．５％，加标回收率为９２～１０８％。     

催化动力学分析法测定痕量碘的研究

基于稀硫酸介质中,痕量碘离子对高碘酸钾氧化甲基绿的催化作用,建立了动力学光度法测定痕量碘离子的新方法,方法检出限０．１４μｇ／ｍｌ,线性范围０～０．５μｇ／ｍｌ,包括其它卤素在内的大多数阴离子,阳离子不干扰测定,用于食盐中碘的测定,结果满意。     

催化光度法测定高压蒸汽中痕量铜的研究

基于稀氨水介质中，Ｃｕ（Ⅱ）催化过氧化氢氧化溴甲酚绿的反应，建立了动力学分光光度法测定高压蒸汽中痕量Ｃｕ（Ⅱ）的新方法。该方法检出限为１．１８×１０－１１ｇ／ｍＬ、线性范围为０～０．０２５μｇ／２５ｍＬ，可用于高压蒸汽中痕量Ｃｕ（Ⅱ）的测定。     

新试剂糠醛缩7-氨基-8-羟基喹啉-5-磺酸的合成及催化荧光测定超痕量钒(Ⅴ)

合成了新试剂糠醛缩７－氨基－８－羟基喹啉－５－磺酸（简称Ｆ７Ｎ８Ｑ５Ｓ）。试剂在酸性介质中被ＫＢｒＯ３氧化分解，生成强荧光物质，Ｖ（Ⅴ）对该反应具有强烈的催化作用，由此建立了测定痕量钒的催化荧光法。反应在酸性介质中进行，０．０５％Ｆ７Ｎ８Ｑ５Ｓ３．０ｍＬ，５％ＫＢｒＯ３０．６ｍＬ，沸水浴中加热２０ｍｉｎ，λｅｘ／λｅｍ＝３２６ｎｍ／３８６ｎｍ。钒的量在０．０～４．８μｇ／Ｌ范围内呈线性，检测限为０．４μｇ／Ｌ。该方法用于实际样品中痕量钒的测定，结果满意。此外还详细研究了反应机理。     

β修正─XO新光度法测定金属材料铜

在ｐＨ＝９氨性溶液中，铜（Ｃｕ￣（２＋））与二甲酚橙（ＸＯ）和ＣＴＢ反应生成紫红色化合物。采用β修正─ＸＯ新光度法测定金属材料中痕量铜。该方法灵敏度高，ＲＳＤ≤５％，加标回收率为９７．２％～１０４．０％，最低检测浓度为０．０２μｇ／ｌ。该方法适合于钢铁等中铜分析。     

催化光度法新指示反应测定痕量铁

研究了在表面活性剂增溶增敏下，邻菲　啉为活化剂，铁催化抗坏血酸还原苯基重氮氨基偶氮苯的褪色反应，建立了测定痕量铁的新方法。检出限为７．４×１０￣（－１１）ｇ／ｍｌ，线性范围为０～１．０μｇ／２５ｍｌ，用于发样、食品样中痕量铁的测定，结果满意。     

高效液相色谱法测定水产品中氟苯尼考和甲苯咪唑的残留量

[方法]建立了高效液相色谱测定水产品中氟苯尼考和甲苯咪唑残留量的分析方法.匀浆样品用碱性乙腈水提取,经盐析和乙腈饱和正己烷除脂后离心净化浓缩,采用高效液相色谱法检测,外标法定量.[结果]当空白样品中氟苯尼考添加水平为0.10～1.00 mg/kg、甲苯咪唑为0.01～0.10 mg/kg时与峰面积呈线性关系,相关系数均大于0.99.该方法的回收率为81.2％～102.4％,相对标准偏差为1.2％～7.5％,定量检出限分别为0.10、0.01 mg/kg.[结论]方法简便、快速、重现性好,适于进出口水产品检测分析.     

C型甲苯咪唑的合成与制备

以对氯苯甲酸为起始原料 ,经硝化、氯化、酮化、氨解、还原、环合、转晶 7步反应完成C型甲苯咪唑的制备 ,总收率以对氯苯甲酸计约 6 3.8%。     

Development of self-microemulsifying drug delivery system of mebendazole by spray drying technology: Characterization,in vitro and in vivo evaluation

In this study, a novel liquid self-microemulsifying drug delivery system (SMEDDS) containing mebendazole was formulated and further developed into a solid form by a spray drying method using Aerosil 200 as the solid carrier. The optimum liquid SMEDDS consisted of Labrafil 2125 CS, Tween 20, and Maisine 35-1 as the oil phase, the surfactant, and the cosurfactant, respectively. The formulated SMEDDS was completely emulsified or dispersed within a minute. All formulations were dissolved within 1 h using 0.1 N HCl as dissolution medium, whereas pure drug was less significantly dissolved in this time period. The droplet size was found to be within 250 nm for solid forms of SMEDDS. Solid state characterization was performed by scanning electron micrograph, differential scanning calorimetry, and X-ray powder diffraction. After oral administration to Wistar rats, mebendazole in the solid SMEDDS resulted in the significant improvement in bioavailability compared with that of pure drug analyzed by RP-HPLC. The optimized formulation showed 24.87 folds increase in bioavailability as compared to pure drug and 8.39 folds increase in bioavailability in comparison to marketed tablet of mebendazole. The optimized batch has found to be 3.1726 years of shelf life. In conclusion, the solid SMEDDS is a promising solid dosage form for poorly water-soluble and low bioavailability drugs.     

Anemias in preschool children: diagnosis, treatment and evaluation, Recife-PE, Brazil]

The diagnosis and the effects of treatment of anemia were assessed in children aged 6-71 months. A total of 1,161 preschool children from a health center of INAMPS (Instituto Nacional de Assistência Médica e Previdência Social) in Recife, Pernambuco, was studied. Hemoglobin was determined by the method described by Hainline. WHO criteria were used to identify anemia. According to their age, nutritional status and family income, the children were divided into groups, and those with anemia were treated with ferrous sulphate and an anti-helminthic (mebendazole). Anemia prevalence was substantially higher in children aged 2 years, and a statistically significant association (0.01 level) was found between anemia and nutritional status and family income. After treatment, hemoglobin values were normal in 40% of the anemic children; simultaneously, mean hemoglobin values increased from 9.11 to 10.3 g/dl which was statistically significant (p less than 0.001). This investigation is part of a collaborative study performed in four Brazilian states to offer "know-how" to a national program for combating iron deficiency anemia.     

The use of Caenorhabditis elegans for anthelmintic screening

Caenorhabditis elegans maintained on ampicillin-treated Escherichia coli has been found to be sensitive to the benzimidazole anthelmintics, albendazole, cambendazole, fenbendazole, flubendazole, mebendazole, oxfendazole, oxibendazole, parbendazole, thiabendazole and the non-benzimidazoles, avermectin B1_a, bitoscanate, febantel, levamisole, morantel, nitroscanate, oxantel, phenothiazine, pyrantel, pyrvinium pamoate, rafoxanide and stilbazium iodide at concentrations of 50 μg cm^?3 or less. It can therefore be used in a high through-put in vitro pre-screen for anthelmintics. It is suggested that C. elegans may also be useful for evaluating the mode of action of drugs and the mechanism of resistance of nematodes to anthelmintics.     

7种杀菌剂防治黄瓜白粉病田间药效评价

为筛选防治黄瓜白粉病的高效、低毒、安全杀菌剂,对戊唑醇等7种杀菌剂进行田间药效试验。结果表明:43%氟菌·肟菌酯SC 192.6 g/hm~2、300 g/L醚菌·啶酰菌SC 270 g/hm~2处理的防效较高,病指防效分别为88.69%和83.30%;430 g/L戊唑醇SC 96.75 g/hm~2、37%苯醚甲环唑WG 90 g/hm~2、36%硝苯菌酯EC 216 g/hm2处理的病指防效分别为78.75%、73.23%和74.55%;250 g/L嘧菌酯SC 337.5g/hm~2、250 g/L吡唑醚菌酯EC 150 g/hm~2处理的防效较差,病指防效分别为49.40%和47.78%。     

Proximal analysis, fatty acids profile, essential amino acids and mineral content in 12 species of fishes of commercial importance in Venezuela

Proximate composition, fatty acid profile, essential aminoacid and minerals were determined in twelve fish species (armadillo, bocachico, cachama, carpeta, corvina, lisa, mero, merluza, pargo, robalo, tilapia and trucha). Proximate analysis: moisture, protein and ash, were performed using AOAC methodology, fat by Bligh and Dyer method, fatty acids by Gas Chromatography, aminoacid using High Performance Liquid Chromatography and minerals by spectrophotometric method. Results showed that moisture varies between 70.49% for Armadillo and 78.64% for Mero, protein between 18.70% for Merluza and 25.53 for Armadillo, ash between 0.94% for Mero and 2.13% for Carpeta and fat between 1.12% for Pargo and 6.15% for Cachama. Unsaturated fatty acids (omega 3) were the most common found for all the spices. Essential amino acids studied were present in all the spices. Tilapia (10.938 g/100 g of fish), Bocachico (9.231 g/100 g of fish) and Mero (8.738 g/100 g of fish) shown greater content of essential amino acids. Phosphorous was the most concentrated mineral with a mean value of 238.13 mg/100 g of fish followed by calcium with 42.11 mg/100 g of fish. It was concluded that all studied species are an excellent source of protein, omega 3 fatty acids and minerals.     

高效液相色谱法测定牛组织中雷复尼特的残留量

采用高效液相色谱法测定牛组织中雷复尼特的残留量。牛组织样品经乙腈提取,中性氧化铝柱净化后采用Kromasil C18柱,以v(乙腈)∶v(pH2.5的水)=83∶17为流动相,紫外检测波长282nm处检测雷复尼特。在0.05～4.0μg/mL内线性关系良好,检测低限为0.01μg/g。高、中、低3个水平添加时肌肉组织中样品的平均回收率分别为78.3%,85.8%和95.1%;肝组织中样品的平均回收率分别为73.3%,83.9%和91.9%;肾组织中样品的平均回收率分别为74.3%、81.9%和92.8%。测试方法快速、简便、准确、灵敏,可用于牛组织中雷复尼特残留量的测定。     

火焰原子吸收光谱法测定芦荟中的钙、镁含量

建立了火焰原子吸收光谱法测定芦荟中钙、镁含量的方法。该方法的检出限量：钙为０－３μｇ／ｇ,镁为０－０２ μｇ／ｇ;平均加标回收率：钙为９９－１％ ,镁为９９－７ ％ ;相对标准偏差：钙为０－６６％ ,镁为０－５２％ 。本方法已用于芦荟鲜叶及芦荟制品中钙、镁含量的测定,结果令人满意。     

细菌纤维素吸附人体内毒素的研究

细菌纤维素湿膜和冷冻干燥的细菌纤维素干膜持水率分别为75.92g/g、9.14g/g,膨胀率分别为72.13mL/g、8.62mL/g。测定了其对人体内有毒物质组胺、氨和NO2-的吸附作用。细菌纤维素湿膜对组胺、氨和NO2-的吸附速率分别为44.83μg/(g·min)、0.83μg/(g·min)、5.28μg/(g·min),吸附率分别为18.6%、18.6%、27.8%。细菌纤维素干膜对组胺、氨和NO2-的吸附速率分别为52.25μg/(g·min)、1.23μg/(g·min)、7.67μg/(g·min),吸附率分别为15.8%、11.5%和23.0%。