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为了提高双孢蘑菇废弃菇柄5’-核苷酸得率,增加其经济价值。以双孢蘑菇废弃菇柄为原料,首先通过单因素试验研究了pH值、酶底比、酶解温度、酶解时间对5’-核苷酸酶解得率的影响,并采用正交试验分析对5’-磷酸二酯酶对核酸的酶解条件进行优化;其次对双孢蘑菇废弃菇柄浸提液中5’-核苷酸在不同pH值、温度和加热时间下的稳定性进行了研究。结果表明,在单因素试验结果基础上,采用正交试验分析对主要酶解参数进行优化,得到双孢蘑菇废弃菇柄核酸酶解的最优参数为:pH5.0、酶底比3000 U/g、酶解温度50℃、酶解时间3 h,5’-核苷酸得率为1.60 g/100 g。通过稳定性试验发现,双孢蘑菇废弃菇柄中5’-核苷酸在pH值为中性偏酸(pH5~7)条件下比较稳定,受热温度越高5’-核苷酸越不稳定,在试验范围内为保留更多的5’-核苷酸,热处理时间以不超过1.5 h为宜。这一结果可为双孢蘑菇废弃菇柄5’-核苷酸的提取及其在烹调加工中的应用提供依据和指导。 相似文献
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为探讨蛋白酶水解双孢蘑菇子实体的最佳工艺条件,本文选用中性蛋白酶、风味蛋白酶、木瓜蛋白酶、碱性蛋白酶和复合蛋白酶对双孢蘑菇子实体进行酶解实验。通过单因素实验、响应面实验研究了酶解温度、酶解时间、p H、加酶量及料水比对双孢蘑菇子实体水解度的影响,优化了其工艺参数。结果表明,酶解温度60℃,酶解时间4h,p H10.5,加酶量2%,料水比1∶12为最优酶解参数组合,验证实验得到双孢蘑菇的水解度达到26.01%。酶解液中可溶性多糖含量及还原糖含量较子实体分别增加了3.95倍和1.91倍,蛋白质含量减少了5.02g/100g。本研究表明酶解双孢蘑菇子实体蛋白可以提高其总体功能性营养价值。 相似文献
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蘑菇酱油酿造工艺初探 总被引:3,自引:0,他引:3
在酱油酿造过程适当的添加双孢蘑菇下脚料,可提高酱油风味品质。试验得出较佳原料配比为面粉:曲精:双孢蘑菇=1:0.02:50,双孢蘑菇比例不宜超过50,否则影响制曲,甚至导致制曲失败;成曲蘑菇:18Be浓盐水=2:5,才能得到良好的发酵结果,酿造出风味较好的蘑菇酱油。通过对成品蘑菇酱油的指标分析并和黄豆酱油等进行对比,蘑菇酱油的各项指标均达到国家一级酱油标准。 相似文献
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为了降低杀菌工艺对扇贝面酱营养品质的破坏程度,研究不同杀菌温度和杀菌时间对扇贝面酱中游离氨基酸的影响,以扇贝面酱为实验材料,采用柱前衍生-高效液相色谱法,测定不同杀菌温度和杀菌时间对扇贝面酱中游离氨基酸含量的影响,结合大肠菌群和致病菌的检测,确定扇贝面酱较适宜的杀菌条件。实验结果表明,杀菌温度、杀菌时间和杀菌温度×杀菌时间的交互作用对游离氨基酸含量的影响均达到显著水平(p<0.05)。较优的杀菌条件为80℃处理10min,在此条件下,扇贝面酱中可检测到18种游离氨基酸,与未杀菌的扇贝面酱相比,游离氨基酸总含量和必需氨基酸总含量均增多,产品微生物指标达到相关产品的国家标准。 相似文献
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Soo Im Chung Se Young Kim Yeong Joo Nam Mi Young Kang 《Food science and biotechnology》2010,19(1):51-56
Effect of king oyster mushroom on the physicochemical and textural properties of cuttlefish surimi gel was investigated. Surimi
gels containing 20, 30, 40, and 50%(w/w) king oyster mushroom were analyzed for color, rheological properties, and sensory
attributes. Texture profile analysis of fish paste added with king oyster mushroom paste showed significant decrease on hardness,
cohesiveness, and gumminess. Springiness of cuttlefish paste increased with the addition of king oyster mushroom paste. The
results of the sensory evaluation showed that cuttlefish paste added with 30, 40, and 50% king oyster mushroom had higher
overall acceptability compared with that of the control. However, flavor, softness, chewiness, and overall quality tended
to decrease in 50% king oyster mushroom paste group. Therefore, this study suggests that king oyster mushroom paste and cuttlefish
were suitable for surimi gel production and could be effective in improving the nutritional properties and functionality of
surimi gel. 相似文献
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在大田条件下连续两年,选用兰考矮早8和豫麦49-198为试验材料,研究不同施硫量和灌水次数对小麦粉质拉伸参数和淀粉糊化特性参数的影响.结果表明:施硫可以改善小麦品质,施硫能使兰考矮早8的稳定时间和拉伸阻力延长,弱化度、最终黏度和稀懈值升高,能使豫麦49-198的稳定时间和拉伸阻力延长,最终黏度、稀懈值和反弹值升高,并达显著或极显著差异;灌拔节水,能延长兰考矮早8的形成时间和稳定时间,提高其拉伸阻力、峰值黏度、最终黏度及反弹值;可以延长豫麦49-198的面团形成时间和稳定时间,提高其拉伸阻力.水、硫互作效应中,60 kg/hm2的硫肥施用量配合灌拔节水能改善兰考矮早8的品质,60 kg/hm2的硫肥施用量配合灌拔节水或不灌水亦能改善豫麦49-198的品质. 相似文献
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香菇面包的制作工艺研究 总被引:2,自引:0,他引:2
以面粉和香菇茵柄粉为原料,通过对面团配比、发酵、醒发以及烘烤等工艺参数的研究,研制出一种新型的营养面包。结果表明,香菇面包的生产工艺条件为:高筋面粉:香菇菌柄粉:糖:酵母为100:2.0:18:1.2,发酵温度为30℃,发酵时间为100min,醒发温度为32℃,醒发时间为130min,烘烤温度为上火190℃,底火200℃,烘烤时间为12min。对香菇面包进行品质评价,产品具有香菇特有的香气,膳食纤维丰富,营养价值高于普通面包。 相似文献
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Aurea M. Almazan 《Journal of the science of food and agriculture》1988,42(1):67-75
The quality of fried chips made from cassava flour depended on total soluble sugar (TSS) concentration and on some paste viscosity parameters of the flour. Chip colour darkened as TSS content increased. Taste was not affected by total HCN content but became bitter as TSS levels increased. Chip HCN concentration was minimal if flour with low HCN was used. Greater surface expansion of the dried chips during frying produced crisper chips. Expansion during frying correlated positively with maximum and setback viscosities of the flour paste. 相似文献
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Noodle characteristics prepared from both 100% of domestic and imported wheat flour (as a control) and blended with 40% of orange-fleshed sweet potato paste were studied. The domestic wheat flour had higher protein content (13.8%), compare to imported wheat flour (11.7%), thus giving the highest protein content (18.86%) in noodle prepared from 100% domestic wheat flour. However its noodle colour was disliked due to a lower whiteness level compared to imported wheat flour. Blended 60% of domestic wheat flour with 40% of sweet potato paste could improve the noodle colour acceptance. The noodles prepared from both 100% wheat flours and blended with 40% sweet potato paste had met the national standard quality for moisture and protein content. This suggests that sweet potato paste is promising for noodle ingredients as a wheat flour substitute. 相似文献
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