高效液相色谱法测定试验鸡肉中水飞蓟宾含量

为建立试验鸡肉中水飞蓟宾含量的高效液相色谱（high performance liquid chromatography,HPLC）检测方法,以乙酸乙酯为提取剂从鸡肉匀浆中提取水飞蓟宾后,采用C18色谱柱,在流动相为水∶甲醇=47∶53（体积比）、柱温30℃、流速0.65 mL/min、检测波长288 nm、进样量20μL的条件下进行分离检测。结果显示,水飞蓟宾标准品浓度在0.1～5.0μg/mL时线性关系良好,标准曲线方程为y=95.16x-0.875 3,相关系数R2=0.999 6,检测试验鸡肉中水飞蓟宾的平均回收率为88.45%～104.34%,相对标准偏差（relative standard deviation,RSD）为1.85%～2.94%。结果表明,该方法简单易行,精密度较高,回收率良好,可用于试验鸡肉中水飞蓟宾含量的测定。     

甲醇超声提取法测定保健食品中维生素A

目的 采用甲醇超声方法提取保健食品中的维生素A。用高效液相色谱法(high performance liquid chromatography, HPLC)法进行含量测定, 并与GB/T 5009.82-2016进行比较, 同时进行方法学验证。方法 样品前处理方法为甲醇超声提取法。采用C18(4.6 mm×250 mm, 5 μm)色谱柱, 甲醇为流动相, 检测波长为325 nm, 流速为1 mL/min, 柱温为30 ℃, 进样量为20 μL。结果 超声时间为5~15 min提取效率最高, 工作曲线在1.00~10.00 μg/mL范围内线性关系良好, 相关系数为0.9998, 相对标准偏差为1.82% (n=6), 平均回收率为101.09%, 方法检出限为10 μg/100 g, 定量限为31 μg/100 g。结论 和国标法相比, 本方法操作简单、准确度高, 适用于保健食品中维生素A含量的测定。     

高效液相色谱法测定蜂胶软胶囊中白杨素和高良姜素的含量

目的建立高效液相色谱法测定保健食品蜂胶软胶囊中白杨素和高良姜素含量的方法。方法样品经乙醇提取后,由C_(18)色谱柱分离,以甲醇-0.1%磷酸(60:40,V:V)为流动相进行等度洗脱,用紫外检测器在波长为270 nm检测,外标法定量。结果白杨素在5.685～284.25μg/mL浓度范围内线性关系良好,相关系数为0.999,加标回收率为97.1%～103.0%,相对标准偏差(relative standard deviation,RSD)为0 8%～1.8%;高良姜素在5.32～266μg/mL浓度范围内线性关系良好,相关系数为0.999,加标回收率为96.2%～102.0%,RSD为0.5%～1.9%。结论此方法简便快速,准确可靠,可提高保健食品蜂胶软胶囊中白杨素和高良姜素含量测定的效率。     

高效液相色谱法测定中国劲酒中桂皮醛的含量

目的建立采用高效液相色谱法(high performance liquid chromatography,HPLC)测定中国劲酒中桂皮醛含量的方法。方法样品以10%甲醇为提取剂,超声提取30 min,以乙腈-水(35:65,V:V)为流动相,流速为1.0 m L/min,采用Shimadzu VP-ODS色谱柱(250 mm×4.6 mm,5μm)分离,在290 nm检测波长下经HPLC检测,同时对流动相比例和提取剂等条件进行优化。结果桂皮醛浓度在0.0216~0.216μg/m L范围内呈良好的线性关系,相关系数为0.99998,平均回收率为98.1%,相对标准偏差为1.63%,方法检出限为0.1μg/m L。结论本方法操作简便﹑结果准确且重现性好,可用于中国劲酒中桂皮醛含量的测定。     

分散液相微萃取-液相色谱法测定奶瓶中的双酚A

建立了分散液相微萃取提取婴儿奶瓶中溶出痕量双酚A(BPA)的方法,并用高效液相色谱测定了其含量。奶瓶浸泡液用0.5 mL氯仿作萃取剂,0.5 mL甲醇作分散剂,以3 500 r/min离心10 min,吸取萃取剂20μL,进样,采用高效液相色谱法(HPLC)测定BPA的含量。色谱柱为Inertsil C18柱(5μm,4.6×150 mm),流动相为甲醇∶水(V∶V)=70∶30,流速为0.8 mL/min,检测波长为280 nm。该方法 BPA在0.05 mg/L~0.25 mg/L范围内线性关系良好(R=0.999 7),BPA检出限(S/N=3)为0.50μg/L。该法用于婴儿奶瓶中BPA含量的检测,测得婴儿奶瓶中双酚A的含量范围在0~18.93μg/L,平均加标回收率为96.44%,RSD为4.57%(n=4)。方法结果满意,可适用于婴儿奶瓶、矿泉水等食品和饮料容器中溶出BPA的含量测定。     

板栗花粉总黄酮提取及HPLC定量

对板栗花粉总黄酮提取工艺进行优化,最佳提取条件:提取温度60℃、提取时间1.5h、乙醇浓度80%、液固比(V/m)20:1。HPLC条件:紫外检测波长280nm、柱温30℃、进样量20μL、流速1mL/min、流动相甲醇:水=75:25(V/V),在此色谱条件下HPLC法测定板栗花粉总黄酮含量为5.00g/100g。      

板栗花粉总黄酮提取及HPLC定量

对板栗花粉总黄酮提取工艺进行优化,最佳提取条件:提取温度60℃、提取时间1.5h、乙醇浓度80%、液固比(V/m)20:1.HPLC条件:紫外检测波长280nm、柱温30℃、进样量20μL、流速1mL/min、流动相甲醇:水=75:25(V/V),在此色谱务件下HPLC法测定板栗花粉总黄酮含量为5.00g/100g.     

超高效液相色谱法测定化妆品中对羟基苯乙酮含量

目的建立超高效液相色谱法(ultra performance liquid chromatography,UPLC)测定化妆品中对羟基苯乙酮含量的分析方法。方法样品前处理以甲醇为溶剂,超声30 min,采用色谱柱Atlantis~?d C_(18)分离,以甲醇-水(25:75,V:V)为流动相,流速为0.2 mL/min,检测波长为275 nm,经UPLC检测;同时对提取剂和提取时间、流动相比例、检测波长等条件进行优化。结果对羟基苯乙酮浓度在0.4376~21.83μg/m L范围内呈良好的线性关系,相关系数为0.9996,膏霜、乳、液等不同剂型样品回收率高,平均回收率分别为99.89%、99.83%和100.24%,RSD分别为0.75%、0.65%和0.46%。方法检出限为1.38μg/g。结论该方法简单、快速、准确,可适用于化妆品中对羟基苯乙酮含量的测定。     

高效液相色谱法检测保健食品中的番茄红素

目的建立高效液相色谱法测定保健食品中番茄红素的分析方法。方法用含有0.1%2,6-二叔丁基-4-甲基苯酚抗氧化剂的甲醇:二氯甲烷(10:90,V:V)作为提取溶液。通过Agilent Eclipse XDB-C_(18)(4.6 mm×250mm,5μm)色谱柱;甲醇:乙酸乙酯(85:15,V:V)作为流动相,二极管阵列检测器,检出波长475 nm,色谱柱温25℃,流速1.0 mL/min,进样体积10μL,作为检测条件。结果番茄红素在0~200μg/mL范围内线性良好,相关系数r=0.9995,最低检出限为0.0815μg/mL,平均回收率在89.7%~109.8%之间,RSD为8.4%。结论本方法可以准确、快速地检测保健食品中的番茄红素。     

超高效液相色谱法(UPLC)快速测定胡椒中胡椒碱含量

建立了胡椒中胡椒碱含量测定的超高效液相色谱(UPLC)测定法。样品经95%乙醇回流提取,以甲醇/水(70/30,V/V)为流动相,流速0.25mL/min,343nm波长进行检测。结果表明:胡椒碱保留时间为2.585min,测定线性范围为0.05～10.0μg/mL,r=0.9998,当添加量为0.4～4.0g/100g时,回收率为90.5%～95.8%,相对标准偏差为0.56%～1.81%,检测限为0.008g/100g。该方法操作简单,灵敏度高,重现性好,省时,适合于胡椒碱含量的测定。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press