Long-term water quality monitoring of the Sejnane reservoir in northeast Tunisia

The Sejnane reservoir in northeast Tunisia provides drinking and irrigation water. Long-term water quality monitoring data including precipitation, evaporation, temperature, pH, conductivity, dissolved oxygen, turbidity, total suspended solids, major anions and cations, fluoride, BOD₅, NO₃ ⁻, NO₂ ⁻, NH₄ ⁺, P _tot, fecal coliform bacteria, boron and heavy metals (Fe, Zn, Cu, Ni, Pb, Cr and Cd) are reported. The appropriateness for irrigation was estimated by the SAR and Na percentage and the water quality assessed using the Canadian Water Quality Index as good to excellent, which confirmed its suitability for drinking, aquatic life and irrigation purposes.      

Assessment of heavy metals contamination and its effects on oyster (Saccostrea cucullata) biometry parameters in the Asaluyeh port coasts,Persian Gulf,Iran

The current study examines biometric parameters and concentration of some heavy metals in soft tissues of Saccostrea cucullata in the Asaluyeh coast, Persian Gulf, Iran. The metals concentration in the soft tissue of the Saccostrea cucullata ranged as: Zn: 765–3411 mg kg^?1, Cu: 120.3–580.6 mg kg^?1, Cd: 0.13–3.5 mg kg^?1, As: 0.39–3.3 mg kg^?1, Pb: 0.05–1.64 mg kg^?1, Ni: 0.02–0.44 mg kg^?1, V: 0.06–0.32 mg kg^?1, Cr: 0.02–0.16 mg kg^?1 and Hg: 0.002–0.068 mg kg^?1. The results showed that the concentration of total heavy metals in oysters of the control station (CA) is usually lower. The results also indicated lack of correlation between oyster biometric parameters and bioaccumulated heavy metals, except between Cu and longitudinal diameter (R = 0.77). The concentration of heavy metals is higher than limits recommended by the World Health Organization, except for Cd and Pb.     

Denitrification en continu a l'aide de microorganismes immobilises sur des supports solidesContinuous denitrification by immobilized cells

In this paper, we describe a study of biological denitrification by immobilized cells. Nitrates are reduced in sterile solutions by Pseudomonas aeruginosa immobilized in a fixed bed reactor, and in synthetic waste water by mixed cultures immobilized into a fluidized bed reactor.The fixed bed reactor is a Plexiglas column filled with corn stovers (Table 1). It is 0.05 m in diameter and 0.55 in height, its volume being approx. 11. The fresh medium is injected at the base of the column and the liquid level is regulated by an overflow weir. Reactor and carrier are sterilized with ethylene-oxide. After sterilization 1 l. of a growing batch culture of Pseudomonas aeruginosa is introduced aseptically and the reactor is then fed continuously (45 ml h^?1) with fresh medium (NNO₃ = 40 mg l^?1) until the first steady state is reached.Nitrates and nitrites are determinated by means of a colorimetric method.Reactor efficiency remains constant for over 40 days. Nitrates and nitrites concentrations are measured inside the reactor for flow varying from 2 to 16 ml min^?1 (Fig. 2). Reductions of nitrates and nitrites seem to be two first-order reactions (Fig. 3 and Table 2) and constant rate increases with flow rate (Fig. 4). Until nitrate concentration reaches 960 mg/l^?1 (NNO₃) degradation is correct (Figs 5 and 6), beyond nitrites, which have been formed, seem to be inhibitor.Using this reactor, 50 mg NNO₃ have been reduced per hour and per liter of empty reactor, but it may be possible to reduce 140 mg NNO₃ l^?1 h^?1 if fresh medium contains 200 mg NNO₃ l^?1.The fluidized bed reactor is a Plexiglas column filled with earthenware. It is 0.05 m in diameter and 3.15 m in height, its volume being approx. 6.201. Fresh medium is injected at the base of the column and the liquid level is regulated by an overflow weir. Figure 7 shows the retention time of the liquid in the reactor in relation to flow. The first steady state has been reached after 2 weeks, and it has not been possible to know half life time of the column.Four experiments were conducted (Table 3) and, for each nitrate, nitrite and methanol concentrations in the reactor were measured (Fig. 8). So, it appears that reduction of nitrates and nitrites are two first-order reactions (Table 4) and that constant rate values, which are higher than in fixed bed reactor, increase with flow.The reactor is more affected by a flow shift than by a nitrate concentration shift in fresh medium, and biomass linked onto carrier is about 76 mg of dry matter g^?1 of earthenware.So, our fluidized bed column is able to reduce 560 mg NNO₃ h^?1 l^?1 of empty reactor, then retention time of liquid is less than 3 min.     

Simultaneous nitrification and denitrification process in a new Tubificidae-reactor for minimizing nutrient release during sludge reduction

Nutrient release is reported as one of the main disadvantage of sludge reduction induced by aquatic worm. In this study, a Static Sequencing Batch Worm Reactor (SSBWR) was proposed with novel structure of perforated panels, combined aeration system and cycle operation. Effective simultaneous nitrification and denitrification were obtained owing to the stratified sludge layer containing aerobic and anoxic microzone formed on each carrier during most of the operation time in the SSBWR, which created suitable conditions for remarkable sludge reduction and nutrient removal. The results showed that the total nitrogen (TN) concentration, NO₃^?–N + NO₂^?–N concentration and NH₄⁺–N release could be reduced by 67.5%, 98.5% and 63.0%, respectively. And the soluble chemical oxygen demand (sCOD) released by sludge predation was also proved to provide a carbon source for denitrification leading to carbon release control and substantial cost savings. A schematic diagram of the stratified sludge layer and the mass balance of the nitrification–denitrification cycle were given, providing further insight into the nutrient (sCOD and nitrogen compounds) transformation during the worm predation in the SSBWR. For the mixed sludge liquid of 3000 mg TSS/L, 30 mg/L sCOD and 40 mg/L NO₃^?–N, the NO₃^?–N and NO₂^?–N came close to zero, and the sludge concentration, NH₄⁺–N release and sCOD release was reduced by 33.6%, 63.0% and 72.5%, respectively, during 48 h’ predation.     

The life cycle impact assessment applied to the Domingo Rubio tidal system by the study of seasonal variations of the aquatic eutrophication potential

The innovative technique of Life Cycle Impact Assessment (LCIA) applied to dynamic environmental systems has been recently developed. In this work we investigate a complex system, the Domingo Rubio tideland (Huelva, Spain), where a tidal marsh and a continental lagoon converge. This wetland, catalogued as Natural Park by the Andalusia government, is subjected to a high eutrophicant pressures related to the strawberry culture and the inputs coming from industrial wastes. NO₂⁻, NO₃⁻ and PO₄³⁻ were analyzed in 41 water samples, obtaining values up to 100 mg L^− 1 Σ(NO₂⁻, NO₃⁻) and 18.5 mg L^− 1 PO₄³⁻. All these values exceed the accepted levels by the European Environment Agency. N/P ratios and the Aquatic Eutrophication Potentials (AEP) for N and P showed a constant imbalance of the system. During one tidal cycle, the tidal channel can have both N and P as limiting nutrient (P is the limiting nutrient during low tide and N is during high tide) and there exists an alternation of AEP domination too between N and P in the continental area, what points to an excess of both nutrients all over the study area, and to the necessity of diminishing the nutrient inputs and a higher control on these pollution sources as well.     

Effects of operational parameters on the treatment of nitrate‐rich wastewater by autohydrogenotrophic denitrifying bacteria

The effects of the C/N ratio, as well as hydrogen gas and substrate concentrations, on the treatment of nitrate‐rich wastewater by autohydrogenotrophic denitrifying bacteria in sequencing batch reactors were investigated. Among the various C/N ratios applied in this study, a C/N ratio of 4 was found to be the most suitable ratio that resulted in faster growth and adaptation. The denitrification rate improved with less accumulation of nitrite with increasing H₂ sparging time. Moreover, an increase in nitrate concentration resulted in high nitrite accumulation. The nitrite accumulated to 601 mg/L NO₂‐N when the nitrate concentration was 1000 mg/L NO₃^– ‐ N. The specific degradation rates for nitrate and nitrite were found to be 27.8 mg NO₃^?‐N/g MLVSS/d and 50 mg NO₂^?‐N/g MLVSS/d, respectively, when the C/N ratio was 2, and H₂ as electron donor was sparged for 1 min with 2 h interval.     

Responses of Cyperus involucratus Rottb. to nitrogen and phosphorus, with reference to wastewater reclamation

There is increasing use of aquatic plants in biosystems to ameliorate wastewaters. Cyperus involucratus Rottb. is a large emergent aquatic plant which may be useful in treating wastewater because it grows quickly, does not die back in winter, has little weed potential and grows in dryland situations. Many wastewaters contain high levels of N and P, and the capacity of C. involucratus to take up these nutrients was studied under glasshouse conditions. Maximum growth was attained between 84 and 168 mg NO₃-Nl⁻¹ and 1–5 mg PO₄-Pl⁻¹. At a low level of supplied NO₃-N, most of the N in plant organs was reduced-N; at levels of supplied NO₃-N above 84 mg l⁻¹, there was considerable accumulation of NO₃-N in plant organs. The accumulation of NO₃-N was greatest in culms where it made up over 50% of their N content when plants were grown on 350 mg NO₃-Nl⁻¹. Linear regression equations best described the relationships between dry matter and reduced-N contents in roots, culms and leaves. Over 60% of the total plant N and P accumulated in above ground organs, and concentrations of up to 0.53% P and 3.4% N (dry wt basis) were recorded in leaves. C. involucratus would be a suitable plant for wastewater reclamation on account of its high dry matter production, its ability to tolerate hyper-eutrophic levels of N and P, and the accumulation of most of the N and P in the harvestable portions of the plant.     

Stormwater impact on water quality of rivers subjected to point sources and urbanization – the case of Addis Ababa,Ethiopia

Urbanization of a catchment often causes degeneration of rivers. We studied the water quality of three rivers in Addis Ababa based on the impact of stormwater and non‐point sources, and urbanization. Along these rivers several point sources were registered, with direct discharge of industrial and domestic wastes into them. To distinguish the impact of these year‐round point‐sources from stormwater, we analysed physicochemical parameters, nutrients and heavy metals sampled from upstream to downstream sections of each river in the dry and wet season. Dissolved oxygen (DO), NO₂–N, NH₄–N, PO₄–P, (Cr(VI) and Cu) exceeded international standards, pointing to a generally poor water quality of the rivers in both seasons. NO₃–N, Mn and Zn were problematic in dry season only. Although stormwater improved DO, conductivity, PO₄–P, Cr(VI) and Zn, the levels were still critical, pointing to construction sites, agriculture and pit latrines, somewhat offsetting the effect of stormwater dilution. No clear impact of urbanization pressure was found.     

The stability of particulate nitrate in the Los Angeles atmosphere

Simultaneous measurements of atmosphere particulate nitrate (NO₃^?) and its gas phase precursor nitric acid (HONO₂) were conducted in Los Angeles during severe smog episodes, and the results were compared to those predicted on the basis of thermodynamic data for the HONO₂ (g) + NH₃ (g) = NH₄ NO₃ (s) equilibrium. Over the wide range of conditions studied (four-hour averaged NO₃^? = 0.5?44.3 μg m^?3 HONO₂ = 1.4?36.0 μg m^?3, T = 10?33°C, humidity = 16?99%, ozone up to 460 ppb) the solid equilibrium model was found to be applicable to only about one-third of the total number of data sets. In this case good agreement was found between measured nitrate values and those expected from comparison of measured and equilibrium nitric acid concentrations. Samples collected at humidities above the deliquescent point of ammonium nitrate at the sampling temperature, for which the solid NH₄ NO₃ equilibrium no longer applies and solution chemistry must be considered, accounted for two-thirds of the data sets. For this subset, good agreement was also found between experimental results and theoretical considerations.     

Fire Emissions into the Atmosphere

The amount of various pollutants per year emitted into the atmosphere from fires in Sweden is estimated. The pollutants considered in detail are CO₂, CO, HCN, NO_x, SO₂, HCl, unburned hydrocarbons, and particulate matter. The emission of N₂O, aromatic hydrocarbons, dioxins, and heavy metals, is discussed qualititavely. The estimate is based on the number of fires in buildings and vehicles in Sweden a typical year. Emissions from wildfires have not been included.The results indicate that the amount of combustible material consumed by fire each year is approximately 12 kton, although it should be noted that during certain years, industrial fires may inflate this number. The emission of particulate matter and unburned hydrocarbons is approximately equivalent to that expected from the combustion of 2 Mton of solid fuel in a combustion plant, corresponding to energy production of approximately 15 TWh, which is of the same order of magnitude as that expected from heavy transport in Sweden each year. The emission of the other species studied is negligible relative to other sources. Measurements of species that have not been studied in detail (such as dioxins) under realistic conditions are necessary for determining the amount of these species emitted per year.     

Application of response surface methodology for simultaneous carbon and nitrogen (SND) removal from dairy wastewater in batch systems

The application of batch systems to the direct treatment of dairy wastewater was investigated. Batch experiments were conducted to study the organics removal and simultaneous nitrification and denitrification (SND) process. The experiment was conducted by varying four independent parameters (mixed liquor suspended solids, chemical oxygen demand [COD]/N ratio, aeration time and cycling time), using a central composite design under response surface methodology. The process responses of five dependent parameters (COD, Total kjehldahl nitrogen (TKN), NO₃ ^?, effluent NO₃ ^? and effluent total nitrogen) were studied, and in each case, the percentage removal in batch runs was determined with each process displayed in contour plots. Finally, after optimizing the process conditions, the best treatment of dairy wastewater under optimized conditions was established and the responses were shown. This study shows that microbial granules cultivated under the alternating aerobic/anaerobic conditions in batch systems could efficiently remove organic carbon and convert all the ammonium to nitrogen gas.     

Isotope tracer methods for investigations of nitrogen deficiency in Castle Lake, California

Previous studies conducted at Castle Lake, California suggested that phytoplankton growth could be limited by low levels of dissolved inorganic nitrogen during the summer growing season. We have used the isotopes H¹⁴CO₃⁻, ¹³NH₄⁺, ¹⁵NO₃⁻, and ¹³NO₃⁻ as tracers to determine the rates of inorganic carbon and nitrogen uptake by the natural phytoplankton community and their response to nitrogen enrichment. Experimental designs followed the procedures of several bioassay techniques which have been developed to investigate algal nutrient deficiency.The results indicate that the phytoplankton were nitrogen deficient throughout the growing season. Ammonium uptake was rate-limited by the external concentration of ammonium. Nitrate uptake was regulated by the availability of nitrate and molybdenum, and potentially by the assimilation of ammonium. Although N-enrichment enhanced photosynthesis in short-term experiments during the early portion of the growing season, the photosynthetic response was initially negative during midseason, and a positive response did not occur until 2–3 days had elapsed. This suggests that a lack of stimulation, or even inhibition, of inorganic carbon uptake upon nitrogen enrichment does not necessarily preclude N-deficiency.     

Inorganic nitrogen release kinetics and exchangeable inorganic nitrogen of the sediments from shallow lakes in the middle and lower reaches of the Yangtze River region

Inorganic nitrogen (IN) release kinetics and exchangeable inorganic nitrogen (EIN) of the sediments from shallow lakes in the middle and lower reaches of the Yangtze River region were investigated. The results showed that the EIN contents of the studied sediments ranged from 131 to 274 mg/kg. With the increase of the total nitrogen (TN) contents, their EIN also significantly increased. Their ratios of EIN to TN ranged from 5 to 17%, and with the increase of their TN contents, their ratios decreased. The relative contribution of NH₄⁺‐N and NO₃^‐N to EIN ranged from 83 and 11% to 89 and 17%, respectively. The curves of the NO₃^‐N release kinetics of the studied sediments were not completely consistent with those of IN and NH₄⁺‐N. The amounts of the IN released from sediments not only relate to their EIN contents, but also to other N fractions.     

A decade’s (2014–2024) perspective on cassava’s (Manihot esculenta Crantz) contribution to the global hydrogen cyanide load in the environment

In recent years, developing countries have increased their cassava (Manihot esculenta) production for food security. Cassava contains cyanogen glycosides, mainly as linamarin, which through bio-catalysis, i.e. enzyme hydrolysis, results in hydrogen cyanide (HCN). HCN is released into the environment through numerous ways with subsequent volatilisation. Thus, the HCN released during the period 2002–2013 was estimated between 0.025?×?10^?3 to 6.71 ppq (African), 0.012?×?10^?3 to 1.01 ppq (Asian) and 0.007?×?10^?3 to 0.920?×?10^?3 ppq (South American). Furthermore, a decade’s (2014–2024) projection of HCN volatilisation displays increases of 60.5% (Africa), 57.7% (Asia) and 50.5% (South America) when compared with the current production. Furthermore, gas released during cassava plants’ growth, i.e. HCN, NH₃, and NO₂, was quantified in healthy plants. Varying concentrations of HCN were released. These further indicated the presence of a pseudo-halogenic gas in the environment – a contributor to climate change.     

Modeling residential indoor concentrations of PM2.5, NO2, NOx,and secondhand smoke in the Subpopulations and Intermediate Outcome Measures in COPD (SPIROMICS) Air study

Increased outdoor concentrations of fine particulate matter (PM_2.5) and oxides of nitrogen (NO₂, NO_x) are associated with respiratory and cardiovascular morbidity in adults and children. However, people spend most of their time indoors and this is particularly true for individuals with chronic obstructive pulmonary disease (COPD). Both outdoor and indoor air pollution may accelerate lung function loss in individuals with COPD, but it is not feasible to measure indoor pollutant concentrations in all participants in large cohort studies. We aimed to understand indoor exposures in a cohort of adults (SPIROMICS Air, the SubPopulations and Intermediate Outcome Measures in COPD Study of Air pollution). We developed models for the entire cohort based on monitoring in a subset of homes, to predict mean 2-week–measured concentrations of PM_2.5, NO₂, NO_x, and nicotine, using home and behavioral questionnaire responses available in the full cohort. Models incorporating socioeconomic, meteorological, behavioral, and residential information together explained about 60% of the variation in indoor concentration of each pollutant. Cross-validated R² for best indoor prediction models ranged from 0.43 (NO_x) to 0.51 (NO₂). Models based on questionnaire responses and estimated outdoor concentrations successfully explained most variation in indoor PM_2.5, NO₂, NO_x, and nicotine concentrations.     

Atmospheric NH3 and NO2 concentration and nitrogen deposition in an agricultural catchment of Eastern China

To assess the atmospheric environmental impacts of anthropogenic reactive nitrogen in the fast-developing Eastern China region, we measured atmospheric concentrations of nitrogen dioxide (NO₂) and ammonia (NH₃) as well as the wet deposition of inorganic nitrogen (NO₃⁻ and NH₄⁺) and dissolved organic nitrogen (DON) levels in a typical agricultural catchment in Jiangsu Province, China, from October 2007 to September 2008_. The annual average gaseous concentrations of NO₂ and NH₃ were 42.2 μg m⁻³ and 4.5 μg m⁻³ (0 °C, 760 mm Hg), respectively, whereas those of NO₃⁻, NH₄⁺, and DON in the rainwater within the study catchment were 1.3, 1.3, and 0.5 mg N L⁻¹, respectively. No clear difference in gaseous NO₂ concentrations and nitrogen concentrations in collected rainwater was found between the crop field and residential sites, but the average NH₃ concentration of 5.4 μg m⁻³ in residential sites was significantly higher than that in field sites (4.1 μg m⁻³). Total depositions were 40 kg N ha⁻¹ yr⁻¹ for crop field sites and 30 kg N ha⁻¹ yr⁻¹ for residential sites, in which dry depositions (NO₂ and NH₃) were 7.6 kg N ha⁻¹ yr⁻¹ for crop field sites and 1.9 kg N ha⁻¹ yr⁻¹ for residential sites. The DON in the rainwater accounted for 16% of the total wet nitrogen deposition. Oxidized N (NO₃⁻ in the precipitation and gaseous NO₂) was the dominant form of nitrogen deposition in the studied region, indicating that reactive forms of nitrogen created from urban areas contribute greatly to N deposition in the rural area evaluated in this study.     

Emissions from power plants fueled by peat,coal, natural gas and oil

Measurements were carried out to investigate the emissions of SO₂, NO_x, fly ash, heavy metals, radioactive elements and polycyclic aromatic hydrocarbons (PAH) from one oil-fired, two coal-fired, five peat-fired and four natural gas-fired power plants or district heating plants.The emissions of sulphur dioxide depended mainly on the sulphur content of the fuel. In the combustion of oil, practically all the sulphur in the fuel reached the atmosphere as sulphur dioxide. When coal and peat were used as fuel, the amounts of sulphur retained in the ash were about 15 and 20%, of the original sulphur content, respectively. The SO₂ emissions from oil, coal, peat and natural gas were typically 1000, 600, 120, and < 10 mg MJ⁻¹, respectively.The emissions of oxides of nitrogen depended on the nitrogen content of the fuel and also on the combustion temperature. The NO_x emissions from plants burning oil, coal peat and natural gas were typically 100, 300, 160 and 100 mg MJ⁻¹, respectively. The lowest emissions were measured in the small natural gas-fired district heating plants (20–30 mg NO_x MJ⁻¹).The emissions of fly ash and various elements depended mainly on the ash content of the fuel and on the efficiency of the dust collectors, which decreases sharply when the power plant output exceeds its nominal value. When the dust separation efficiency is 99% for coal- and peat-fueled power plants, and when there are no dust collectors in oil-fueled power plants, the typical fly ash emissions are 45, 20 and 25 mg MJ⁻¹, respectively. Characteristic emissions of heavy metals and natural radioactive elements were greatest in the combustion of coal, with the exception of vanadium and nickel, of which the greatest emissions were from oil; the greatest emissions of ²¹⁰Po were from peat.The PAH emissions of the different fuel-fired power plants were, with one exception, similar (2–10 μg MJ⁻¹). Emissions from the circulating fluidized bed combustion of peat were about 10 times (40 μg MJ⁻¹) those of the other methods of combustion.     

Present limitations and future prospects of stable isotope methods for nitrate source identification in surface- and groundwater

Nitrate (NO₃⁻) contamination of surface- and groundwater is an environmental problem in many regions of the world with intensive agriculture and high population densities. Knowledge of the sources of NO₃⁻ contamination in water is important for better management of water quality. Stable nitrogen (δ¹⁵N) and oxygen (δ¹⁸O) isotope data of NO₃⁻ have been frequently used to identify NO₃⁻ sources in water. This review summarizes typical δ¹⁵N- and δ¹⁸O-NO₃⁻ ranges of known NO₃⁻ sources, interprets constraints and future outlooks to quantify NO₃⁻ sources, and describes three analytical techniques (“ion-exchange method”, “bacterial denitrification method”, and “cadmium reduction method”) for δ¹⁵N- and δ¹⁸O-NO₃⁻ determination. Isotopic data can provide evidence for the presence of dominant NO₃⁻ sources. However, quantification, including uncertainty assessment, is lacking when multiple NO₃⁻ sources are present. Moreover, fractionation processes are often ignored, but may largely constrain the accuracy of NO₃⁻ source identification. These problems can be overcome if (1) NO₃⁻ isotopic data are combined with co-migrating discriminators of NO₃⁻ sources (e.g. ¹¹B), which are not affected by transformation processes, (2) contributions of different NO₃⁻ sources can be quantified via linear mixing models (e.g. SIAR), and (3) precise, accurate and high throughput isotope analytical techniques become available.     

The stabilization of “dissolved” iron in freshwaters

Laboratory experiments have been conducted to see what substances are capable of holding iron at a concentration of about 1 ppm in a “dissolved” form (i.e. will pass through a 0.45 μm pore size filter) in oxygenated water. The results show that reagents capable of doing this include humic and tannic acids, surfactants such as sodium dodecyl sulphate and sodium dodecylbenzene sulphonate, and the inorganic ions silicate and phosphate. In contrast, the synthetic polymer polyvinylpyrrollidone and the simple ions Cl⁻, HCO₃⁻, SO₄⁻ and NO₃⁻ showed no ability to stabilise iron. The efficiency of phosphate at keeping iron in the “dissolved” state was found to decrease in the presence of cations, particularly divalent ones, but increased with rise in water pH in the range 6–11.It seems unlikely that much of the stabilization observed for any of the reagents tested is due to their forming complexes with the iron. A much more likely explanation is that the substances for which positive results were obtained are able to stabilize fine colloidal iron particles and inhibit them from forming larger aggregates.     

Erratum to “Sources of nitrate and ammonium contamination in groundwater under developing Asian megacities”

The status of nitrate (NO₃⁻), nitrite (NO₂⁻) and ammonium (NH₄⁺) contamination in the water systems, and the mechanisms controlling their sources, pathways, and distributions were investigated for the Southeast Asian cities of Metro Manila, Bangkok, and Jakarta. GIS-based monitoring and dual isotope approach (nitrate δ¹⁵N and δ¹⁸O) suggested that human waste via severe sewer leakage was the major source of nutrient contaminants in Metro Manila and Jakarta urban areas. Furthermore, the characteristics of the nutrient contamination differed depending on the agricultural land use pattern in the suburban areas: high nitrate contamination was observed in Jakarta (dry fields), and relatively lower nutrients consisting mainly of ammonium were detected in Bangkok (paddy fields).The exponential increase in NO₃⁻-δ¹⁵N along with the NO₃⁻ reduction and clear δ¹⁸O/δ¹⁵N slopes of NO₃⁻ (∼ 0.5) indicated the occurrence of denitrification. An anoxic subsurface system associated with the natural geological setting (e.g., the old tidal plain at Bangkok) and artificial pavement coverage served to buffer NO₃⁻ contamination via active denitrification and reduced nitrification.Our results showed that NO₃⁻ and NH₄⁺ contamination of the aquifers in Metro Manila, Bangkok, and Jakarta was not excessive, suggesting low risk of drinking groundwater to human health, at present. However, the increased nitrogen load and increased per capita gross domestic product (GDP) in these developing cities may increase this contamination in the very near future. Continuous monitoring and management of the groundwater system is needed to minimize groundwater pollution in these areas, and this information should be shared among adjacent countries with similar geographic and cultural settings.