3G技术背景下的移动测控网络

根据企业移动信息化的需求构建移动测控网络。采用3G移动通信技术与现有成熟的无线通信技术相结合,建立信息高速传输通道;通过智能终端设备进行信息采集和调用;以数据、声音和图像等多种形式做为信息载体,把企业设备状态参数、工业现场视频、监控系统界面等大量数据实时传送到需要的任何位置,使企业管理人员和技术人员可以随时、随地、随身获取和处理信息。     

Structure analysis of small proteins by electron microscopy: valinomycin, bacitracin and low molecular weight cell growth stimulators.

Dark field electron microscopy was combined with optical filtering to study at high resolution the structure of the cyclopeptide antibiotics, bacitracin and valinomycin, and two proteins of unknown structure, LMW-CSA N and B, low molecular weight granulocyte colony stimulating activity isolated from medium conditioned with normal or leukemic leukocytes. For bacitracin and valinomycin the images faithfully represented the known structural features at a resolution of 0.5 nm or better, depicting a two-ring structure for bacitracin, as well as the position of the potassium ion in valinomycin. Both proteins of unknown structrue had at least one cyclic peptide portion. LMW-CSA N had a size of 2.0 nm, LMW-CSA B of 2.4 nm. A potential site of the calcium ionophoric activity in the latter protein was found to be in the larger of the two ring portions constituting the molecule.     

Instrumentation control and automation in the control of biological effluent treatment.

The role of instrumentation, control, and automation (ICA) in the management, operation, and control of domestic and industrial wastewater treatment works is examined following an historical account of developments in the UK over the past two decades. Included also are aspects of process optimization and process selection, taking into account size and types of works and populations served. The particular ICA requirements of individual unit processes are examined and the effects of unattended operation and remote supervision are considered. Particular attention is paid to measurement technology, the shortcomings of available instruments and systems, and recent developments of relevance, with particular emphasis being placed on microelectronic and fibre- and electro-optic-based sensors and on the role of advanced commenting methods including expert systems. Progress with biologically based sensors is also discussed.     

MD175G10型塔式起重机起升调速系统

ＭＤ175Ｇ10型塔式起重机 (以下简称塔机 )是法国波坦公司生产的一种用于房屋建筑的塔式起重机 ,最大起重量为 10ｔ,最小起重力矩为 1750ｋＮ·ｍ ,起重臂长 50ｍ ,其安装方式可采取附墙式、固定式及大车行走式等多种形式。该塔机的调速系统颇具特色 ,为双电动机合成调速系统。1　调速系统的组成如图 1所示 ,塔机的调速系统由 2台绕线转子电动机 (变速比为 1∶2 )、减速器、卷筒、 2个电磁制动器、限位开关、电阻箱和电气控制柜等组成。2　调速系统的工作原理该系统为双电动机调速系统。塔机的提升、下降各有 5个挡位。其中第 1、 2、 3挡是…     

模糊自整定PID在单品炉温度控制中的仿真

针对焦炉温度大惯性、纯滞后、非线性和时变性等特点,结合PID和模糊控制两者的优点,提出了一种模糊自整定PID控制方法.对模糊自整定PID算法进行了理论分析,对焦炉生产的简化模型做了仿真研究.结果表明,采用模糊自整定PID控制,系统的调节时间缩短,响应速度加快,抗干扰能力和适应参数变化的能力都优于常规PID控制,具有更好的动态特性和稳定性,有效减少了炉温的波动.     

RHO GTPases in the control of cell morphology, cell polarity, and actin localization in fission yeast.

The fission yeast Schizosaccharomyces pombe undergoes morphogenetic changes during both vegetative and sexual cell cycles that require asymmetric cell growth and actin cytoskeleton reorganisations. Different complex signal transduction pathways participate in S. pombe morphogenesis. The Rho family of GTPases are present in all eukaryotic cells, from yeast to mammals, and their role as key regulators in the signalling pathways that control actin organisation and morphogenetic processes is well known. In this review, we will briefly summarize the role of the Rho GTPases in the establishment and maintenance of cell polarity and growth of S. pombe. As in other fungi, S. pombe morphogenesis is closely related to cell wall biosynthesis, and Rho GTPases are critical modulators of this process. They provide the coordinated regulation of cell wall biosynthetic enzymes and actin organisation required to maintain cell integrity and polarised growth.     

Micro-scale chromophore-assisted laser inactivation of nerve growth cone proteins

Directed growth cone movement is crucial for the correct wiring of the nervous system. This movement is governed by the concerted actions of cell surface receptors, signaling proteins, cytoskeleton-associated molecules, and molecular motors. In order to investigate the molecular basis of growth cone motility, we applied a new technique to functionally inactivate proteins: micro-scale Chromophore-Assisted Laser Inactivation [Diamond et al. (1993) Neuron 11:409-421]. Micro-CALI uses laser light of 620 nm, focused through microscope optics into a 10-microm spot. The laser energy is targeted via specific Malachite green-labeled, non-function-blocking antibodies, that generate short-lived protein-damaging hydroxyl radicals [Liao et al. (1994) Proc Natl Acad Sci USA 91:2659-2663]. Micro-CALI mediates specific loss of protein function with unachieved spatial and temporal resolution. Combined with time-lapse video microscopy, it offers the possibility to induce and observe changes in growth cone dynamics on a real time base. We present here the effects of the acute and localized inactivation of selected growth cone molecules on growth cone behavior and morphology. Based on our observations, we propose specific roles for these proteins in growth cone motility and neurite outgrowth.     

用快原子轰击质谱研究醌式化合物Medermycin、G－15F和G－15G

Ｍｅｄｅｒｍｙｃｉｎ、Ｇ－１５Ｆ和Ｇ－１５Ｇ三个化合物都有醌式结构。在底物甘油中，准分子离子为（Ｍ＋３Ｈ）＋，但在底物３－硝基苯甲醇（ＮＢＡ）中，准分子离子为（Ｍ＋Ｈ）＋。本文用ＦＡＢＭＳ对此作了研究。     

Electrospray ionization mass spectrometry of oligonucleotide complexes with drugs, metals, and proteins.

I. Introduction 61 II. Binding of Small Molecules to DNA 62 A. Covalent Binding 62 B. Reversible (Noncovalent) DNA-Binding Agents 65 III. DNA-Metal Ion Complexes 67 A. Platinum Complexes 70 B. Other Metal Ions 73 IV. DNA-Protein Complexes 74 A. Introduction 74 B. ESI-MS of DNA-Protein Complexes 76 C. ESI-MS Analysis of Proteolytic Products of DNA-Protein Complexes 79 D. ESI-MS of Ternary DNA-Protein-Ligand Complexes 80 V. Conclusions 80 Abbreviations 81 References 81 --Interactions of DNA with drugs, metal ions, and proteins are important in a wide variety of biological processes. With the advent of electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI), mass spectrometry (MS) is now a well-established tool for the characterization of the primary structures of biopolymers. The gentle nature of the ESI process, however, means that ESI-MS is also finding application for the study of noncovalent and other fragile biomolecular complexes. We outline here the progress, to date, in the use of ESI-MS for the study of noncovalent drug-DNA and protein-DNA complexes together with strategies that can be employed to examine the binding of small molecules and metal complexes to DNA. In the case of covalent complexes with DNA, sequence information can be derived from ESI-MS used in conjunction with tandem mass spectrometry (MS/MS) and/or enzymatic digestion. MS/MS can also be used to probe the relative binding affinities of drugs that bind to DNA via noncovalent interactions. Overall, the work in this area, to date has demonstrated that ESI-MS and MS/MS will prove to be valuable complements to other structural methods, offering advantages in terms of speed, specificity, and sensitivity. (c) 2001 John Wiley & Sons, Inc.