Contribution of tomato phenolics to suppression of COX-2 expression in KB cells

Tomatoes, which are consumed worldwide, contain abundant phenolics. The objective of this study was to understand the suppression effect of phenolics in fresh and heated tomatoes on the expression of cyclooxygenase 2 (COX-2). Both small and big tomatoes of fresh or heated (in boiling water for 30 min) treatments were used. Sephadex LH-20 gel was used to separate the noncondensed tannin containing and the condensed tannin containing fractions from the crude phenolic extracts of tomatoes. The condensed tannin containing fraction was rich in condensed tannins and simple phenolics. The noncondensed tannin containing fraction contained abundant nontannin flavans. This study explored the effect of tomato phenolic extracts on the regulation of 12-o-teradecanoylphorbol-13-acetate (TPA)-induced inflammatory responses in KB cells. HPLC showed that tomato phenolic profiles were similar between small and big tomatoes either by fresh or heated treatment. Fresh tomato extracts had 70.8 +/- 4.8% (mean +/- SD) noncondensed tannin containing polyphenols (6.68 +/- 0.09 mg/g dry weight), 27.4 +/- 6.9% condensed tannin containing polyphenols (3.52 +/- 0.24 mg/g dry weight), and 1.7 +/- 0.6% other residues. Instead, heated tomato had 53.3 +/- 4.3% noncondensed tannin containing polyphenols (2.70 +/- 0.20 mg/g dry weight), 24.2 +/- 1.7% condensed tannin containing polyphenols (7.37 +/- 0.03 mg/g dry weight), and 22.5 +/- 4.8% other residues. Cell studies showed that phenolic extracts of heated tomatoes resulted in increased suppression of COX-2 expression compared with that of fresh tomato. Noncondensed tannin containing fraction of fresh tomato greatly suppressed COX-2 expression (P < 0.05) that compared to the negative control, but both noncondensed tannin containing and condensed tannin containing fractions of heated tomatoes showed suppression on COX-2 expression. These results suggest that tomato phenolics may play an important role in the chemoprevention of cancer.     

Bog Bilberry (Vaccinium uliginosum L.) Extract Reduces Cultured Hep-G2, Caco-2, and 3T3-L1 Cell Viability,Affects Cell Cycle Progression,and Has Variable Effects on Membrane Permeability

ABSTRACT: Bog bilberry (Vaccinium uliginosum L.) is a blue-pigmented edible berry related to bilberry (Vaccinium myrtillus L.) and the common blueberry (Vaccinium corymbosum). The objective of this study was to investigate the effect of a bog bilberry anthocyanin extract (BBAE) on cell growth, membrane permeability, and cell cycle of 2 malignant cancer cell lines, Caco-2 and Hep-G2, and a nonmalignant murine 3T3-L1 cell line. BBAE contained 3 identified anthocyanins. The most abundant anthocyanin was cyanidin-3-glucoside (140.9 ± 2.6 μg/mg of dry weight), followed by malvidin-3-glucoside (10.3 ± 0.3 μg/mg) and malvidin-3-galactoside (8.1 ± 0.4 μg/mg). Hep-G2 LC50 was calculated to be 0.563 ± 0.04 mg/mL, Caco-2 LC50 was 0.390 ± 0.30 mg/mL and 0.214 ± 0.02 mg/mL for 3T3-L1 cells. LDH release, a marker of membrane permeability, was significantly increased in Hep-G2 cells and Caco-2 cells after 48 and 72 h compared to 24 h. The increase was 21% at 48 h and 57% at 72 h in Caco-2 cells and 66% and 139% in Hep-G2 cells compared to 24 h. However, 3T3-L1 cells showed an unexpected significant lower LDH activity (P ≤ 0.05) after 72 h of exposure corresponding to a 21% reduction in LDH release. BBAE treatment increased sub-G1 in all 3 cell lines without influencing cells in the G2/M phase. BBAE treatment reduced the growth and increased the accumulation of sub-G1 cells in 2 malignant and 1 nonmalignant cell line; however, the effect on membrane permeability differs considerably between the malignant and nonmalignant cells and may in part be due to differences in cellular membrane composition.     

大孔树脂法纯化覆盆子中黄酮化合物

目的 覆盆子是药食两用资源,山奈酚-3-O-芸香糖苷为其中的代表性黄酮。通过比较不同大孔树脂及纯化条件对覆盆子提取物中黄酮的纯化率,确定最佳的覆盆子黄酮纯化条件。方法 以覆盆子总黄酮的吸附率和解吸率为指标,筛选出最佳的大孔树脂及分离纯化参数;利用HPLC分析纯化物中的黄酮种类和含量。 结果 从D101、NAK-9、AB-8、HPD 500、HZ 806、S-8筛选出最适的分离纯化树脂AB-8,最佳分离纯化条件为：液体样品与树脂量比为0.6：1（V/M）,吸附时间为4 h,最适解吸溶剂为70%乙醇（V/V）,洗脱液体积为样品量的4倍,静态解吸时间为4 h。经大孔树脂纯化后,覆盆子黄酮纯度较纯化前提高了3.3倍。通过HPLC分析,纯化物中黄酮以山奈酚-3-O-芸香糖苷和椴树苷为主,含量分别为0.059 mg/g-PDS和0.0046 mg/g-PDS,占覆盆子原料干重的0.032%和0.024%。结论 本研究所得纯化方法可大幅提高覆盆子黄酮提取物的纯度,为覆盆子提取物在营养健康食品中的应用提供参考和借鉴。     

Effective cell separation utilizing poly(N-isopropylacrylamide)-grafted polypropylene membrane containing adsorbed antibody

We previously developed a cell separation method using a poly(N-isopropylacrylamide)-grafted polypropylene (PNIPAAm-g-PP) membrane containing an adsorbed monoclonal antibody (mAb). The purpose of this study is to elucidate the cell separation mechanism in detail and to design an optimal method. As the grafting yield of PNIPAAm increased, the level of the adsorption of IgG(1) and cell adhesion to the membrane decreased. After BSA was adsorbed to a PNIPAAm-g-PP membrane at 6 degrees C, where PNIPAAm was hydrophilic, a small amount of IgG(1) was adsorbed to the membrane at 37 degrees C, where PNIPAAm was hydrophobic. The desorption of the adsorbed IgG(1) was not enhanced even though temperature was reduced to 10 degrees C, where PNIPAAm was hydrophilic. These results indicate that the antibody adsorbed to the intact PP surface of the membrane predominantly contributes to the capture of target cells through the antigen-antibody reaction and that a thermoresponsive transition of PNIPAAm contributes to the detachment of the captured cells. The total number of cells recovered from a PNIPAAm-g-PP membrane containing the adsorbed mAb decreased as the grafting yield increased. A PNIPAAm-g-PP membrane with a 1.7% grafting yield containing adsorbed anti-human CD34 mAb enriched CD34-positive KG-1a cells to 85% from a 1:1 cell suspension of KG-1a cells and CD34-negative Jurkat cells.     

有机碳源对单针藻细胞生长、油脂积累和光合作用的影响

研究不同有机碳源对单针藻(Monoraphidium sp.)细胞生长,油脂积累和光合作用的影响,探讨其细胞生长和油脂积累的最佳碳源浓度。结果表明单针藻具有利用有机碳源进行混合营养生长的能力,葡萄糖、蔗糖对其细胞生长、总脂含量和光合放氧速率具有明显的促进作用,甘氨酸能够促进细胞生长,但总脂含量下降,乙酸钠则表现为抑制作用;在BG-11培养基中加入5g/L葡萄糖后,细胞生物量、总脂产量和光合放氧速率分别为7.8、3.2g/L和240.3μmolO2·(mg·chla)-1·h-1,是同等光合自养条件下的5.6、8.0和1.3倍;单针藻细胞生长、总脂积累的最佳葡萄糖添加浓度为10g/L。     

Assessing sequestration of selected polycyclic aromatic hydrocarbons by use of adsorption modeling and temperature-programmed desorption

Sequestration of phenanthrene and pyrene was investigated in two soils--a sandy soil designated SBS and a silt-loam designated LHS--by combining long-term batch sorption studies with thermal desorption and pyrolysis of amended soil samples. The Polanyi-based adsorption volume and the adsorbed solute mass increased with aging for both soils, thus demonstrating the mechanism for observed sequestration. Despite rigorous thermal analysis, 30-62% (SBS sand) and 8-30% (LHS silt-loam) of phenanthrene could not be recovered after 30-270 days of sorption, with the increase in desorption resistance showing greater significance in SBS sand. For both soils, these values were 20-65% of adsorbed phenanthrene mass. Activation energies estimated from the temperature-programmed desorption (TPD) of sorbed phenanthrene at < or = 375 degrees C were 51-53 kJ/mol, consistent with values derived for desorption of organic compounds from humic materials. The activated first-order model fitting of observed TPD data supports the conclusion that the desorption-resistant fraction of phenanthrene has become sequestered onto condensed organic domains and requires temperatures exceeding 600 degrees C to be released. The work demonstrates the use of thermal analysis in complementing the Polanyi-based adsorption modeling approach for assessing the mechanistic basis for sequestration of organic contaminants in soils.     

纳米Fe2O3靶向鸡肉腐败菌光催化抑菌效能 特性研究

目的探索可见光下纳米Fe2O3对鸡肉腐败菌的光催化抑菌效能特性及机制。方法以Escherichia coli及鸡肉腐败菌Pseudomonas fluorescens和Macrococcus caseolyticus为受试菌株,可见光照为激发条件,研究纳米Fe2O3对细菌菌落数量和菌体脂质氧化程度的影响。结果纳米Fe2O3在可见光下能够有效抑制E.coli、P.fluorescens和M.caseolyticus生长繁殖,1.2 mmol/L的H2O2能够增强Fe2O3的光催化抑菌活性;当Fe2O3质量浓度为0.4 g/L时,对P.fluorescens和M.caseolyticus的抑菌作用效果最强;在光催化过程中,P.fluorescens和M.caseolyticu的脂质氧化值随着反应时间延长呈先升高后降低的趋势,在120 min分别达到最大值1.31nmol/mg(细胞干重)和2.14 nmol/mg(细胞干重)。结论可见光条件下,M.caseolyticus对光催化反应比P.fluorescens更加敏感,纳米Fe2O3先引起细菌体内脂质氧化,进而导致菌体细胞破裂而死亡。     

明胶微球的制备及其对多酚的吸附效果

采用乳化法制备明胶微球,利用显微镜和扫描电镜进行形态表征,研究了明胶微球对不同种类多酚的吸附及解吸附作用,考察了明胶微球的重复使用率。结果表明：在pH 3.5、35 ℃条件下吸附120 min时,明胶微球对表没食子儿茶素没食子酸酯（epigallocatechin gallate,EGCG）、安石榴苷和原花青素表现出良好的吸附作用,而对绿原酸吸附能力弱。用蒸馏水80 ℃处理120 min,微球上吸附的多酚可较好地解吸附。当微球多酚质量比为5∶1时,除绿原酸外,其他多酚的吸附率约54%～78%、单位明胶微球多酚吸附值约107～156 μg/mg、解吸率约60%～75%。明胶微球重复利用5 次后对EGCG的吸附率和解吸率仍然可达55%以上,但重复利用8 次后微球吸附性能急剧下降。因此,明胶微球在食品中多酚的去除和回收中具有潜在的应用价值。     

大孔树脂分离纯化南极磷虾壳中的虾青素

研究AB-8大孔吸附树脂对虾青素的静态、动态吸附效果。结果表明,AB-8树脂对虾青素的最大吸附量为476.2 μg/g干树脂,乙酸乙酯对虾青素的解吸率为98.7%;动态吸附条件为虾青素上样质量浓度2 μg/mL、上样流速4 BV/h。此条件下,皂化液中虾青素的回收率为78.9%,纯度为92.4%。     

酵母菌谷胱甘肽合成能力比较及催化条件优化

试验收集培养了9株酵母菌,分别测定他们的细胞生物量和胞内谷胱甘肽(GSH)含量,酿酒酵母SP004细胞干重达到11.39g/L,啤酒酵母TS013胞内GSH含量达到1.5282mg/g湿细胞;验证考察了4种不同提取方法对细胞内GSH含量测定的影响,结果表明,温差破碎法提取效果较好.同时比较9株酵母菌催化合成GSH的能力,结果显示,絮凝酵母SP5 GSH合成酶活力相对较高,酶活达到2.385mg/g湿细胞,每克湿细胞催化前体氨基酸(AA)合成GSH转化率为7.76％.用单因素试验和正交试验分析法研究不同反应液体积与菌体量比、硫酸镁浓度、磷酸钾缓冲液浓度以及葡萄糖浓度对絮凝酵母SP5细胞催化前体AA合成GSH的影响,结果表明,合成GSH的适宜条件为:谷氨酸60mmol/L、半胱氨酸20mmol/L、甘氨酸20mmol/L、七水合硫酸镁20mmol/L、葡萄糖0.5mol/L、pH值为7.0磷酸钾缓冲液100mmol/L,30℃下,160r/min,振荡反应时间6h.在此条件下,絮凝酵母SP5细胞GSH合成酶活力平均达到2.9498mg/g湿细胞,每克絮凝酵母SP5湿细胞催化前体AA合成GSH的转化率平均为9.60％.     

Regulation and improvement of triterpene formation in plant cultured cells of Eriobotrya japonica Lindl

Loquat (Eriobotrya japonica Lindl) is a traditional Chinese medicinal plant that contains triterpenes, which have been shown to exhibit pharmaceutical activities. In this study, we investigated various different culture conditions for cultured cells of loquat to produce triterpenes, including illumination, carbon source, nutrient composition and culture system. When cultured on 2.5 mg/l of 6-benzyladenine, 1 mg/l of naphthalene acetic acid and 30 g/l of sucrose at 25 ± 2 °C in the dark for 30 days, the nutrient composition significantly regulated the cell growth and triterpene production. Supplied with the Murashige and Skoog medium reached higher level of dry weight (1.27 ± 0.09 g per flask) and total triterpene production (151.54 ± 12.58 mg/g of cultured cells), and the N6 medium produced tormentic acid but inhibited other triterpene products, while the B5 medium produced relatively high corosolic acid. Also found, suspension cultures of loquat cell could achieve high productivity as callus culture.     

Volatile and non-volatile compounds in irradiated semi-rigid crystalline poly(ethylene terephthalate) polymers

In this study two diVerent semi-rigid crystalline and oriented polyethylene terephthalate materials were used and were irradiated at 25-kGy dose at room temperature by using a caesium¹³⁷ radiator. Volatile and nonvolatile compounds present in the irradiated materials were identified and quantified. The qualitative results obtained from HS/GC/TCD/FID analysis at room temperature showed volatiles could not be identified. The HS/GC/MSD analysis performed at 106°C showed that the irradiation generated 668-742 μg/kg formic acid, 868-922 μg/kg acetic acid, 17-32 μg/kg 1,3-dioxolane, and 47-71 μg/kg 2-methyl-1, 3-dioxolane based on PET weight. The results obtained from the thermal desorption and GC/MSD performed at 200°C showed that 10-12mg/kg acetaldehyde, 479-975 μg/kg 1,3-dioxolane, and 6.6-11.2mg/kg methyl-1, 3-dioxolane were detected after irradiation. The concentrations of the two dioxolanes found from thermal desorption were much higher than those observed in the HS, although formic and acetic acids were not detected. It is possible that the formic and acetic acids produced by irradiation underwent further reactions with ethylene glycol during thermal desorption to form the dioxolanes. The soluble solid extracted from various PET specimens before and after irradiation were in a range of 0.67-0.78%. PET cyclic trimer is the major component and is present at 0.41-0.50%, accounting for more than 50% of the percent total solid in PET. Statistically, irradiation did not increase the soluble solid and cyclic trimer. The overall results suggest that 25-kGy irradiation had a significant eVect on increasing the volatile but not the non-volatile compounds detected in the PET specimens.     

坛紫菜不同溶剂组分的抗氧化活性

为研究坛紫菜酚类化合物的抗氧化活性,通过提取和萃取得到坛紫菜4 种溶剂组分：石油醚、乙酸乙酯、正丁醇和水溶性组分,对其DPPH 自由基清除率和还原力大小进行比较,并研究其中多酚和黄酮类化合物的分布。结果表明：4 种溶剂组分在0.2～1.5g/L 质量浓度范围内与抗氧化活性均存在显著剂量效应关系,其DPPH 自由基清除率和还原力大小均为：乙酸乙酯＞正丁醇＞石油醚＞水溶性组分。在质量浓度为1.5g/L 时,乙酸乙酯组分的还原力吸光度为1.97,大于80% 茶多酚(1.90),接近于BHT(2.00)。对多酚类化合物和黄酮类化合物而言,同样是在乙酸乙酯组分中分布最多(分别为69.84mg GAE/g md 和299.49mg RE/g md),在水溶性组分中最少(分别为11.1mgGAE/g md 和32.36mg RE/g md),因此酚类化合物主要分布在中等极性溶剂中,而不是强极性溶剂中。研究还发现,1g/L 坛紫菜4 种溶剂组分的多酚类化合物含量与DPPH自由基清除率、还原力间的相关系数为：r =0.9311 和r=0.7530,而黄酮类化合物含量与DPPH 自由基清除率、还原力间的相关系数为r=0.8899 和r=0.7211,可见坛紫菜多酚类化合物为其抗氧化的主要活性成分。     

Development of chitosan-magnetite aggregates containing Nitrosomonas europaea cells for nitrification enhancement

A cell suspension of the nitrifying bacterium Nitrosomonas europaea obtained after 96-h cultivation was subjected to magnetic separation using chitosan-conjugated magnetite particles (chitosan-magnetite), which have the ability to form aggregates with microbial cells. An equilibrium condition was obtained at room temperature after 30 min and over 90% of the cells were recovered when the chitosan-magnetite concentration was 200 mg/l. The relationship between the cell concentration in the supernatant in equilibrium and the number of cells adsorbed per 1g chitosan-magnetite was expressed by a Freundlich-type adsorption equation. A high nitrifying bacterium activity yield was obtained with a chitosan-magnetite concentration between 100 and 200 mg/l. Repeated batch culture resulted in more N. europaea cells accumulating on the aggregates and as a consequence their nitrification activity improved further. The chitosan-magnetite/cell aggregates were recovered and employed to remove ammonia from artificial wastewater together with PVA-alginate gel beads containing the denitrifying bacterium Paracoccus denitrificans. A higher ammonia removal rate was achieved under aerobic conditions in comparison with that obtained when N. europaea and P. denitrificans were coimmobilized in PVA-alginate gel beads.     

Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts

Crataegus (Hawthorn) has long been used as a folk medicine and is widely utilized in pharmaceutical preparations mainly because of its neuro- and cardiosedative actions and its low toxicity. The pharmacological effects of Crataegus have mainly been attributed to the polyphenolic contents. In this study, the production of polyphenols by ten-year-old Crataegus monogyna calli was studied in relation to growth variation and antioxidant capacity within a subculture period. Assays based on the Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and stability in oil-in-water emulsion were used to characterize the antioxidant actions of the callus cultures. High TEAC (3.66 micromol/g dry weight) and FRAP (208.19 micromol Fe2+/g dry weight) values were observed when maximal growth was reached(days 30-35), and this seemed to be influenced by optimum total phenol (47.40 mg/g dry weight), proanthocyanidin (20.81 mg/g dry weight), flavonoid (7.01 mg/g dry weight), anthocyanin (6.18 mg/g dry weight), (-)-epicatechin (1.77 mgl/g dry weight), procyanidin B2 (3.97 mg/g dry weight), and chlorogenic acid (1.11 mg/g dry weight) production during that period. The TEAC values were strongly associated with total flavonoids and to a lesser extent with total phenols, anthocyanins and total proanthocyanidins. The FRAP antioxidant values correlated to total phenols, proanthocyanidins and flavonoids, respectively. The polyphenolic rich calli were as effective as butylated hydroxytoluene (BHT) in preventing hydroperoxide and conjugated diene formation in a 30% oil-in-water emulsion prepared with stripped sunflower oil, during 7days storage at 30 degrees C. Crataegus monogyna cell culture represents an important alternative source for natural antioxidants.     

Removal and recovery of lithium using various microorganisms

The accumulation of lithium by microorganisms was examined. Among the 70 strains of the 63 species tested (20 bacteria, 18 actinomycetes, 18 fungi, and 14 yeasts), a high lithium accumulating ability was exhibited by strains of the bacteria, Arthrobacter nicotianae and Brevibacterium helovolum. Lithium accumulation by A. nicotianae cells was strongly affected by the pH of the solution. The amount of accumulated lithium was maximum at pH 6. Cells immobilized with polyacrylamide gel also adsorbed lithium. They could be reused during repeated adsorptions, and adsorbed 548 micromol of lithium/g dry wt. cells. The adsorbed lithium was quantitatively and easily desorbed with 1 M hydrochloric acid using a column system.     

Adsorption and desorption of phenanthrene on carbon nanotubes in simulated gastrointestinal fluids

Adsorption of phenanthrene on carbon nanotubes (CNTs) and bioaccessibility of adsorbed phenanthrene were studied in simulated gastrointestinal fluids. Adsorption of phenanthrene on CNTs was suppressed in pepsin (800 mg/L) solution (gastric) and bile salt (500 and 5000 mg/L) fluids (intestinal). In addition to competitive sorption, pepsin and high-concentration bile salt (5000 mg/L, above critical micelle concentration) solubilized phenanthrene (3 and 30 times of the water solubility, respectively), thus substantially reduced phenanthrene adsorption on CNTs. Pepsin and bile salts also increased the rapidly desorbing phenanthrene fraction from CNTs. The rapidly desorbing phase lasted less than 1 h for all CNTs. Further, 43-69% of phenanthrene was released from CNTs after desorption in the simulated gastric and intestinal fluid at low bile salt concentration while 53-86% was released in the gastric and intestinal fluid at high bile salt concentration. These findings suggest that the release of residual hydrophobic organic compounds from CNTs could be enhanced by biomolecules such as pepsin and bile salts in the digestive tract, thus increasing the bioaccessibility of adsorbed phenanthrene and possibly the overall toxicity of phenanthrene associated CNTs.     

Box-Behnken响应面设计优化微波辅助提取酵母胞内海藻糖的工艺

以安琪葡萄酒高活性干酵母为原料,以三氯乙酸(TCA)为浸提溶剂,以海藻糖得率为衡量指标,通过微波法灭酶得出:海藻糖酶适宜灭活方法为微波灭酶119W、45s;通过Box-Behnken响应面设计优化葡萄酒活性干酵母中海藻糖的提取工艺,得到最优工艺参数为:三氯乙酸浓度0.43mol/L、三氯乙酸用量41.95mL、提取时间88.75min、提取温度34.19℃.经回归分析表明:回归方程的R2=95.61％,R2λdj=91.22％,预测值为253.45mg/g dry cell.经验证,在最优提取工艺条件下,葡萄酒高活性干酵母中海藻糖得率为251.86mg/g dry cell,回归模型的预测值与实测值的相对误差＜1％.     

Removal of bromate from drinking water using the ion exchange membrane bioreactor concept

Bromate is a disinfection byproduct with carcinogenic properties that has to be removed from drinking water to concentrations below 10 or 25 microg/L. This work evaluates the applicability of the ion exchange membrane bioreactor (IEMB) concept for the removal of bromate from drinking water, in situations where nitrate is also present in concentrations up to 3 orders of magnitude higher than bromate. The batch results obtained show that the biological reduction of bromate was slow and only occurring after the complete reduction of nitrate. The specific bromate reduction rates varied from 0.027 +/- 0.01 mg BrO3(-)/g(cell dry weight) x h to 0.090 mg BrO3(-)/ g(cell dry weight) x h for the studied concentrations. On the other hand, transport studies, using anion exchange membranes showed that Donnan dialysis could efficiently remove bromate from polluted waters. Therefore, the use of a dense, nonporous membrane in the IEMB system, isolates the water stream from the biological compartment, allowing for the uncoupling of the water production rate from the biological reduction rate. The IEMB system was used for the treatment of a polluted water stream containing 200 microg/L of BrO3(-) and 60 mg/L of NO3(-). The concentrations of both ions in the treated water were reduced below the recommended levels. No bromate accumulation was observed in the biocompartment of the IEMB, suggesting its complete reduction in the biofilm formed on the membrane surface contacting the biocompartment. Therefore, the IEMB has proven to be a technology able to solve specific problems associated with the removal of bromate from water streams, since it efficiently removes bromate from drinking water even in the presence of nitrate, a known competitor of bromate biological reduction, without secondary contamination of the treated water by cells or excess of carbon source.     

GC-MS/MS法测定间接包装材料中18种光引发剂向改性聚苯醚模拟物的迁移量

为了研究间接包装材料中光引发剂(PIs)的迁移规律,建立了采用改性聚苯醚(MPPO)作为模拟物的迁移实验方法,并通过气相色谱-串联质谱(GC-MS/MS)联用技术测定了18 种PIs 的特定迁移量。间接包装材料中迁移出的PIs 被MPPO 吸附后,用乙腈萃取,萃取液用正己烷-乙酸乙酯(3:7,体积比)进行溶剂置换后,经有机滤膜过滤和DB-5MS 弹性毛细管色谱柱分离后,采用串联质谱法分析,内标法定量。结果表明:①18 种PIs 在26 min 内有效分离,且在0.01~5.00 mg/L 范围内线性关系良好(R2>0.997)。加标回收率在86.4%~123.8%之间,相对标准偏差(RSD)<8.3%,检出限(LOD)为0.001~0.021 mg/kg。②迁移量在0.02~30.00 μg/g 时,该测试方法相对重复性标准偏差在6.3%~21.4%之间,相对再现性标准偏差在13.5%~22.3%之间。③实际样品PIs 的迁移率与其分子量和沸点显著负相关。④真空镀铝内衬纸可以有效地阻隔PIs 的迁移。