白雪花对肿瘤细胞增殖的影响研究

[目的]研究白雪花提取物对舌癌细胞增殖的影响.[方法]将舌癌细胞预培养1 d后,将白雪花提取物按不同的浓度分别加入各培养组中,分别培养2～5 d后,用酶标仪测定细胞增殖情况,计算白雪花提取物对舌癌细胞的抑制率.同时,使用Hoechest33342荧光染色法测定细胞核变化情况.[结果]随着白雪花提取物浓度的增大和作用时间的延长,白雪花提取物对舌癌细胞增殖的抑制作用更加明显.当药物浓度达到0.8 mg/ml时,显微镜下几乎见不到完整的细胞核.[结论]白雪花提取物可以有效抑制舌癌细胞增长,导致肿瘤细胞凋亡.     

6种植物提取物的抑菌活性研究

[目的]研究大蒜、石榴、苍耳等6种植物提取物时几种常见蔬菜病害病原菌的抑制作用.[方法]采用生长速率法测定6种植物丙酮抽提物对病原菌菌丝生长的抑制效果,并进一步开展大蒜粉、苍耳提取物防治番茄早疫病的田间试验.[结果]大蒜、石榴提取物对多种植物病原菌的菌丝都有明显的抑制作用;苍耳次之;辣椒提取物主要抑制黄瓜炭疽病菌和番茄早疫病菌;蒲公英提取物主要对黄瓜炭疽病有抑制作用;侧柏叶对黄瓜白粉病菌的抑制率较高.1.5%大蒜粉防治番茄旱疫病效果较好,防效均在95%以上;其中,以2 000倍效果最佳,对成株期番茄早疫病的防治效果迭99.40%.[结论]6种植物提取物中,大蒜提取物的抑菌活性最强,1.5%大蒜素粉剂对番茄旱疫病的防治效果最佳.     

臭牡丹提取物对病原真菌抑菌效果的研究

[目的]研究臭牡丹不同部位的不同溶剂提取物对常见植物病原真菌的抑制效果.[方法]采用菌饼法研究臭牡丹的抑菌能力.[结果]臭牡丹提取物对小麦赤霉、杨树溃疡、棉花枯萎、番茄灰霉病菌均具有不同程度的抑制作用,其中,茎的蒸馏水提取物对杨树溃疡病菌的抑制效果最好,其最小抑菌浓度(M/C)为15mg/ml;茎的80%乙醇提取物对小麦赤霉病菌的抑制效果最好,其MIC为30mg/ml.臭牡丹提取物在pH4～8的范围内具有不同程度的抑菌作用.[结论]臭牡丹提取物对4种病原真菌具有较好的抑制效果.     

中剂量螺旋藻多糖和银杏提取物的联合抗衰老作用研究

[目的]研究中剂量螺旋藻多糖和银杏提取物的联合抗衰老作用.[方法]每天对试验小鼠颈下注射D-半乳糖125 mg/kg,连续42d,建立衰老模型.给药组小鼠每天灌服100 mg/kg螺旋藻多糖、银杏叶及复合药液,观察中剂量复合螺旋藻多糖和银杏叶有效成分对D-半乳糖致衰老小鼠的作用,并测定免疫器官重量、血清SOD活性和心肌、脑丙二醛(MDA)含量等相关衰老指标.[结果]螺旋藻多糖和银杏提取物均能有效对抗D-半乳糖所致小鼠衰老,二者的复合药效要强于单一药效.[结论]该研究为螺旋藻多糖和银杏提取物的联合抗衰老作用提供了试验依据.     

硼替佐米对白血病细胞株HEL抑制作用的初步探讨

目的 探讨硼替佐米单独或联合化疗药物对急性白血病细胞株HEL生长的影响及其机制.方法 MTT法检测药物对HEL的生长抑制效应,流式细胞术检测细胞周期变化及凋亡,Western blotting检测凋亡及细胞周期相关蛋白表达.结果 硼替佐米对HEL细胞的生长抑制作用呈浓度依赖关系,其半数抑制浓度(IC50)为7.15 nmol/L;硼替佐米联合化疗药物明显增强抑制HEL细胞生长;流式细胞术检测可见时间依赖性的G2/M周期阻滞;Western blotting检测显示bcl-2表达下降,bax、p27表达增高,联合用药时具有相加效应,但不管是单独或联合用药,p53蛋白的表达均无明显改变.结论 硼替佐米可能是一种有效治疗急性白血病的药物,与柔红霉素联合应用有更强的抑制肿瘤细胞生长和诱导其凋亡的作用,其机制可能与bcl-2,bax及p27蛋白的调节有关,是非p53依赖性的.     

rAAV/CEA转染树突状细胞诱导特异性CTL杀伤MCF-7细胞系CD44+ CD24-/low乳腺癌干细胞

目的:携带癌胚抗原(carcinocmbryonic antigen,CEA)基因的重组人腺相关病毒(reconstructive human adeno-association virus,rh-AAV)感染树突状细胞(dendritic cell,DC)诱导获得抗原特异性细胞毒性T淋巴细胞(cytotoxic lymphocyte,CTL),体外检测其对CD44+CD24-/low乳腺癌干细胞的杀伤效果.方法:分离供者外周血单个核细胞,以细胞因子白介素-4(interleukin-4,IL-4)、粒细胞-巨噬细胞克隆刺激因子(granulocyte-macrophage colony-stimulating factor,GM-CSF)和肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)诱导培养获得DC,细胞因子白介素-2(interleukin-2,IL-2),刺激培养获得T淋巴细胞.含CEA基因的rh-AAV感染培养DC,诱导成熟后将DC和T细胞混合培养获得CTL细胞.流式分选MCF-7和MA-MDB-231细胞系中CD44+CD24-/low乳腺癌干细胞,MTT法检测CTL细胞对CD44+CD24-/low乳腺癌干细胞的杀伤效果.结果:MCF-7和MDA-MB-231中CD44+/CD24-/low群比例分别为5.1%和76.3%.CEA基因转染DC诱导的CTL细胞对表达CEA抗原的MCF-7杀伤率为46.5%±15.0%,与未转染组相比,差异有统计学意义(P=0.009);对MCF-7细胞中分选的CD44+CD24-/low乳腺癌干细胞的杀伤率为44.7%±28.2%,明显高于未转染组.对非乳腺癌干细胞杀伤率为50.6%±22.2%,与未转染组相比,差异有统计学意义(P=0.05).在不表达CEA基因的MDA-MB-231乳腺癌细胞,CEA转染诱导的CTL细胞对未分选细胞、分选的CD44+/CD24-/low亚群和非干细胞亚群的杀伤率与未转染的对照组相比,差异均无统计学意义(P＞0.05).结论:携带CEA基因rh-AAV转染DC诱导产生的抗原特异性CTL细胞可杀伤表达CEA的乳腺癌细胞,对CD44+CD24-/low乳腺癌干细胞也具有一定的杀伤活性,提示免疫治疗可能是治疗乳腺癌干细胞潜在有效的手段.     

32种中草药抑菌活性的比较研究

[目的]研究连翘(FRUCTUS FORSYTHIAE)、黄连(RHIZOMA COPTIDIS)和刺五加(RADIX ET RHIZOMA SEU CAULIS ACAN-THOPANACTS SENTICOSI)等32味中草药提取物的抑菌作用.[方法]利用95%乙醇提取药物的有效成分,以大肠杆菌和金黄色葡萄球菌为指示菌,采用牛津杯法测定中草药提取物的抑菌作用,[结果]32种中草药中,虎杖等26种中草药提取物对金黄色葡萄球菌具有抑制作用;连翘等23种中草药提取物对大肠杆菌具有抑制作用.且抑菌效果在一定浓度范围内随药液浓度的增大而增强.其中,连翘和黄连对大肠杆菌的最低抑菌浓度分别是4和10 mg/ml,对金黄色葡萄球菌的最低抑菌浓度分别是6和8 mg/ml.[结论]虎杖和连翘分别对金黄色葡萄球菌和大肠杆菌具有较好的抑制效果.     

镧对镉胁迫羽衣甘蓝的调节效应

[目的]为花卉植物用于Cd污染土壤栽培提供依据.[方法]采用水培试验研究了镧(La)对羽衣甘蓝受到镉(Cd)胁迫的调节效应.[结果]低浓度的Cd对羽衣甘蓝的出芽率、株高、根系、地上和地下鲜重与干重等指标均有一定的抑制作用,高浓度的Cd对其有明显的抑制作用,而一定浓度的La可以缓解这种抑制作用.[结论]5mg/L La缓解高浓度Cd对羽衣甘蓝种子出芽以及生长等各项指标的抑制作用效果最好.     

稀土Y3+对铜绿微囊藻生长和生理特性的影响

[目的]探讨不同浓度钇(Y3+)对铜绿微囊藻(Microcystis aeruginosa)生长特性的影响和藻细胞超微结构的变化.[方法]以铜绿微囊藻FACHB912为试材,采用生理和生化方法研究了不同浓度的外源稀土Y3+(0、0.05、0.10、0.20、0.50、1.00、2.00、5.00和10.00 mg/L)对藻细胞生长的影响,并比较了藻细胞叶绿素a、丙二醛(MDA)的含量及其超微结构随不同浓度Y3+胁迫的变化.[结果]相对低浓度Y3+(0.05～0.20 mg/L)对铜绿微囊藻生长表现出明显促进作用,而高浓度Y3+(0.50～10.00 mg/L)则部分或完全抑制了藻细胞的正常生长;Y3+对铜绿微囊藻的叶绿素a合成、MDA也有影响.叶绿素a随Y3+浓度的提高呈现先上升后下降的变化趋势;低浓度Y3+(0.05～0.20 mg/L)对铜绿微囊藻MDA含量无显著影响,然而随着Y3+浓度的增加和胁迫时间的延长,藻细胞中MDA含量显著上升.[结论]一定低浓度的Y3+可有效促进铜绿微囊藻的生长和藻细胞叶绿素a等生理指标的上升.     

信号分子N-乙酰高丝氨酸内酯(AHLs)诱导铜绿微囊藻细胞凋亡的研究

[目的]研究信号分子N-乙酰高丝氨酸内酯(AHLs)与铜绿微囊藻细胞凋亡的关系.[方法]以铜绿微囊藻为试验材料,采用5μmol/L AHLs处理铜绿微囊藻细胞,用DAPI染色后观察细胞凋亡的形态.[结果]AHLs在I μmol/L浓度下能诱导铜绿微囊藻的细胞凋亡,从而抑制铜绿微囊藻的生长和增殖.[结论]该研究结果为治理铜绿微囊藻水华爆发提供了科学依据.     

转炉炉壳热应力分析

根据实际转炉建立实体模型。以有限元为手段,考虑了炉衬和炉壳材料的物性参数随温度变化的特点和炉衬与炉壳之间膨胀间隙,计算了转炉炉壳在温度载荷和炉衬膨胀压力同时作用下的热应力。所得结果可为转炉炉壳设计提供依据。     

焦炭热性能指标意义浅析

通过对焦炭在高炉内的历程及其变化规律的剖析,阐述焦炭热性能指标的意义,形象地把焦炭在高炉内的损耗分为三部分(M1,M2,M3)。指出M2对焦比及焦炭价格有一定的指导作用。     

Results of treatment of cancer of the cardia

Cancer of the cardia is traditionally discussed with cardiac cancer of the lower portion of the esophagus and upper gastric cancer invading the esophagus, and the specific characteristics of cancer of the cardia have never been clearly defined. We reviewed the outcome of 172 patients with adenocarcinoma of the cardia who had undergone radical surgery between 1949 and 1994 in the Division of Gastrointestinal Surgery of the Cancer Institute Hospital. The centers of the tumors were located within 2 cm above and below the boundary between the esophagus and the stomach, and their longitudinal diameter was less than 8 cm. We divided the patients into an early period (1949-1979; n = 79) and a late period (1980-1994; n = 93), and focused on the historical transition. There were no differences in patient gender or histology between the two periods. However, the late period was associated with fewer cases of esophageal invasion and shorter longitudinal diameter, as the age of the population advanced. The number of advanced cancers such as the localized and infiltrative type had decreased, and early cancer and early clinical stage had become more common, but despite the fact that the number of early cancers had increased, extended dissection, such as thoracic and paraaortic lymph node dissection, was performed more frequently. By clinical stage, the long-term outcome markedly improved in Stage I patient in the late period, and tended to improve in Stage II and III patients. This appears to have been attributable to the prevention of micrometastasis by extended dissection, although the number of early cancers is another major potential cause. There were no differences in the outcome of Stage IV patients between the two periods, and further advances in multimodality therapy must be awaited. The range of resection is basically proximal gastrectomy, and if there are adequate indications, the prognosis is favorable. In view of the status of lymphatic flow and lymph node metastasis, and long-term results, lateroaortic lymph node dissection is important. Since the number of early cancer patients has been increasing, if intraabdominal recurrence is prevented, intrathoracic lymph node dissection will contribute greatly to the outcome of such patients.     

The effect of amount of possession of the trait of hostility on accuracy of perception of hostility in others.

Some theories of personality hold that an individual's perception of others is influenced by his own personality characteristics; more specifically, that a trait of low social value will be projected more into others, and that insight into the possession of these characteristics reduces this distortion. These 2 hypotheses were tested by having a group of college students rate each other (including themselves) on degree of friendliness or hostility. Agreement with group rating about self yielded the insight score. In general, the hypotheses were supported; the "friendly" Ss were most accurate in their perception of others, and insight was positively associated with accurate perception. From Psyc Abstracts 36:04:4HL16M. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effect of antibiotics of chemotaxis of human leukocytes

The effect of 20 different antibiotics on chemotaxis by human neutrophils was studied. Human leukocytes incubated with chloramphenicol, rifampin, sodium fusidate, and tetracyclines in vitro showed markedly depressed migration. The mechanisms by which these antibiotics affect leukotaxis are discussed.     

Quality of life of relatives of mental patients

Usage of quality of life of psychiatric patients' relatives as the parameter of estimation of the quality of psychiatric care is considered. By means of standard questionnaire elaborated by the authors as well as by means of both individual and group conversations the quality of life was analyzed in 40 relatives which lived together with psychiatric patients. Different spheres of their life and difficulties (financial, social-psychologic, production) were examined in families with such patients. The defects of psychiatric care that had direct influence on the quality of life of the psychiatric patients' relatives are outlined.