Development and application of a new method for evaluating shape controllability of rolling mills for flat products

A new method is developed for quantifying the shape controllability of rolling mills for flat products. The method enables one to acquire a comprehensive and accurate assessment of shape control performance in such a way that both the adjusting range and the ability to bear undesirable rolling force fluctuations can be efficiently articulated, understood and studied. A concise discussion concluding the formulation of the evaluation system is offered regarding the general applicability of it. To demonstrate the feasibility of our approach, an example application is presented to the adjustment characteristics analysis of a six-high mill for its three fast acting actuators. Significant differences are found in the effects of work stability changes on the stand’s capability to counter quadratic and quartic terms of flatness deviations. The underlying factors which are likely to be responsible for such differences and an unfavourable condition that may arise in actual production are also discussed.     

Development and application of a new method for assessing job performance in behavioral/economic terms.

Describes the development of a method for estimating the standard deviation of job performance in dollars that might permit wider application of utility analysis to personnel activities. The method builds on traditional industrial psychological principles of job analysis and performance measurement, and it allows translation of behaviorally based performance rating data into economic terms. In a field study of 602 1st-level managers, promoted either via a panel selection/interview process or via an assessment center, the method was shown to be feasible, practical, and simple to use. Comparative utility analysis indicated a significant payoff in terms of improved performance per person per year for the assessment center. (26 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Proof of hepatitis A virus negative-sense RNA by RNA/DNA-hybrid detection: a method for specific detection of both viral negative- and positive-strand RNA species

The detection of hepatitis A virus (HAV) negative-strand RNA, which is synthesized during replication of the positive-strand RNA genome, proved to be difficult. We developed a method for the specific detection of HAV negative-strand RNA by RNA-DNA hybridization and luminescence detection using an anti-RNA:DNA hybrid antibody. This method, which is also applicable for the specific detection of positive-strand RNA, offers a simple, yet relatively rapid and certain means of detecting low amounts of RNA such as HAV negative-strand RNA. By using appropriate hybridization DNA probes, the method should be applicable for the detection of single-stranded RNA species of different viruses in general.     

单层双面自清理悬臂弹性筛网振动筛的结构及应用

简述了悬臂弹性筛网、单层双面悬臂弹性筛网和自清理装置的结构原理,对振动筛在莱钢750m3高炉上的应用进行了总结.实践表明,该筛具有结构合理、筛分效率高、运行可靠、寿命长等特点.     

A new computational method for detection of chimeric 16S rRNA artifacts generated by PCR amplification from mixed bacterial populations

A new computational method (chimeric alignment) has been developed to detect chimeric 16S rRNA artifacts generated during PCR amplification from mixed bacterial populations. In contrast to other nearest-neighbor methods (e.g., CHECK_CHIMERA) that define sequence similarity by k-tuple matching, the chimeric alignment method uses the score from dynamic programming alignments. Further, the chimeric alignments are displayed to the user to assist in sequence classification. The distribution of improvement scores for 500 authentic, nonchimeric sequences and 300 artificial chimeras (constructed from authentic sequences) was used to study the sensitivity and accuracy of both chimeric alignment and CHECK_CHIMERA. At a constant rate of authentic sequence misclassification (5%), chimeric alignment incorrectly classified 13% of the artificial chimeras versus 14% for CHECK_CHIMERA. Interestingly, only 1% of nonchimeras and 10% of chimeras were misclassified by both programs, suggesting that optimum performance is obtained by using the two methods to assign sequences to three classes: high-probability nonchimeras, high-probability chimeras, and sequences that need further study by other means. This study suggests that k-tuple-based matching methods are more sensitive than alignment-based methods when there is significant parental sequence similarity, while the opposite becomes true as the sequences become more distantly related. The software and a World Wide Web-based server are available at http://www-hto.usc.edu/software/mglobal CHI.     

Development and application of a new hot-work die steel for hot stamping

A new hot-work die steel for hot stamping was developed, and used the die for mass production. The produced die showed good performance owing to its high heat conductivity and wear-resistant characteristics. Two different benchmarking hot-work die steels were investigated, and then compared in terms of their impact ductility,temper characteristics, heat conductivity, and thermal stability. The result of the high-temperature friction wear test indicated that oxidative wear was the main mode in high temperature. On the basis of the comparison and test results, the alloying composition of the new hot-work die steel was especially designed. The new die steel showed good performance with good wear-resistant quality, as well as temper hardness and heat conductivity of HRC 50 and34.3 W/(m·K), respectively. Furthermore,without surface plasma nitriding, the die made of the new steel had no obvious galling with 6142 strokes. After surface plasma nitriding, the die completed 40000 strokes with good surface. The die life is expected to exceed 200000 strokes.     

还原性中间包覆盖剂的开发与应用

针对目前中间包覆盖剂不能去除因连铸过程异常而进入中间包钢水的氧的问题,开发了一种具有还原性质的连铸用中间包覆盖剂。与常规覆盖剂相比,该覆盖剂能还原去除中间包钢水内的过量溶解氧,起到净化钢水、改善连铸保护浇铸的作用,并具备常规覆盖剂的一切积极功能。阐述了该还原性中间包覆盖剂的主要组分和理化性能。该覆盖剂的应用,解决了连铸保护浇铸异常等因素引起的中间包钢水氧量过高的问题。     

一种板形检测值处理方法的开发及应用

介绍了一种用于接触式分段板形仪的板形检测信号处理方法及处理流程.用该方法处理板形检测信号不需要计算带钢在板形仪上形成的包角.该法编制成程序后,作为一个模块被应用在一台可逆冷轧机的板形控制系统中.轧机投入运行后,生产稳定,并取得了良好的板形控制效果,平均板形偏差控制在5 I以下,表明该方法是稳定、有效的,可以用于工业生产.     

Evaluation of a dipstick method for the detection of human immunodeficiency virus infection

Serology has been a fundamental tool to prevent post-transfusional infection with human immunodeficiency virus (HIV) and for epidemiological surveys, the first step to attempt control of the pandemia. Enzyme immunoassay is in widespread use. Nevertheless, simpler methods are needed in many countries, where laboratory facilities and trained personnel are limited, and HIV prevalence is high. The evaluation of a simple and noninstrumented HIV antibody test is presented here. The test employs synthetic antigens of HIV-1 and HIV-2 attached to the teeth of a polystyrene comb, which fit into the wells of standard microtiter plates where samples are diluted. Captured antibodies are developed with colloidal gold-labeled Protein A. Three seroconversion panels plus 662 samples were tested, including HIV-1 and HIV-2-infected individuals, normal blood donors, and a noninfected baby born to a seroreactive mother. When compared with enzyme-linked immunosorbent assay (ELISA) and Western blot, the dipstick showed 100% sensitivity and 98.7% specificity. The simplicity of result evaluations and excellent reagent stability make the dipstick suitable for small blood banks and for epidemiological surveys.     

PCR amplification of the fimA gene sequence of Salmonella typhimurium, a specific method for detection of Salmonella spp

The goal of this study was to evaluate the suitability of the fimA gene amplification by PCR as a specific method for detection of Salmonella strains. Salmonella typhimurium and other pathogenic members of the family Enterobacteriaceae produce morphologically and antigenically related, thin, aggregative, type 1 fimbriae. A single gene, fimA, encodes the major fimbrial unit. In order to obtain higher specificity, we have selected a series of primers internal to the fimA gene sequence and have developed a PCR method for detecting Salmonella strains. A collection of 376 strains of Salmonella comprising over 80 serovars, isolated from animals and humans in Canada, have been used to evaluate this PCR method. Forty non-Salmonella strains were also tested by the same procedure. Cultures were screened by inoculating a single colony of bacteria directly into a PCR mixture containing a pair of primers specific for the fimA gene. The specific PCR product is an 85-bp fragment which was visualized by polyacrylamide gel electrophoresis and ethidium bromide staining. All Salmonella strains gave positive results by the PCR. Feed and milk samples contaminated by Salmonella strains were also detected by this procedure. The detection of all Salmonella strains tested and the failure to amplify the fragment from non-Salmonella strains confirm that the fimA gene contains sequences unique to Salmonella strains and demonstrate that this gene is a suitable PCR target for detection of Salmonella strains in food samples.     

漏钢预报新技术研发及应用

介绍了目前国内外现有的几种漏钢预报识别方法,分析其原理及应用情况,提出了中冶连铸的漏钢预报模型及该新技术在数学方法、计算机技术、自动化控制应用方面的特点,并介绍了近期该新技术较好的应用效果。     

Structure of a neutralizing antibody bound monovalently to human rhinovirus 2

The structure of a complex between human rhinovirus 2 (HRV2) and the Fab fragment of neutralizing monoclonal antibody (MAb) 3B10 has been determined to 25-A resolution by cryoelectron microscopy and three-dimensional reconstruction techniques. The footprint of 3B10 on HRV2 is very similar to that of neutralizing MAb 8F5, which binds bivalently across the icosahedral twofold axis. However, the 3B10 Fab fragment (Fab-3B10) is bound in an orientation, inclined at approximately 45 degrees to the surface of the virus capsid, which is compatible only with monovalent binding of the antibody. The canyon around the fivefold axis is not directly obstructed by the bound Fab. The X-ray structures of a closely related HRV (HRV1A) and a Fab fragment were fitted to the density maps of the HRV2-Fab-3B10 complex obtained by cryoelectron microscope techniques. The footprint of 3B10 on the viral surface is largely on VP2 but also covers the VP3 loop centered on residue 3064 and the VP1 loop centered on residue 1267. MAb 3B10 can interact directly with VP2 residue 2164, the site of an escape mutation on VP2, and with VP1 residues 1264 to 1267, the site of a deletion escape mutation. Deletion of these residues shortens the VP1 loop, moving it away from the MAb binding site. All structural and biochemical evidence indicates that MAb 3B10 binds to a conformation epitope on HRV2.     

A PCR-RFLP method for the detection and species identification of human microsporidia

Amputation in a growing child should be performed through a joint and not above or below a joint, as is commonly the case in an adult. We describe a technique for performing a through-knee amputation in a situation in which a soft-tissue sarcoma involved a leg, reaching up to the knee joint. Soft-tissue dissection was performed above the knee, leaving a safe zone from the tumor. The distal femur was dissected free of all attachments to the tibia and leg, leaving it intact but protruding from the soft-tissue sleeve of the thigh. To be able to close the stump over the protruding distal femur, the femur was shortened in the metaphysodiaphyseal area. Follow-up over 3 years shows a good through-knee stump, tumor free, and a normal distal growth plate.     

Detection of human papillomavirus type-16 DNA utilising microtitre-plate based amplification reactions and a solid-phase enzyme-immunoassay detection system

The development of a nested polymerase chain reaction (PCR) assay to detect low concentrations of human papillomavirus type-16 (HPV-16) DNA for epidemiological studies is described. The PCR utilises primers located in the E5 open reading frame, has an analytical sensitivity of 4 HPV-16 genomes and does not produce amplicons from other common genital HPVs (types-6, -11, -18, -31 and 33). This assay was carried out in 96-well plates utilising internal primers labelled with dinitrophenol (DNP) and biotin so that amplicons can be captured onto streptavidincoated plates and detected using an alkaline phosphatase-labelled monoclonal antibody to DNP. The assay was effective for detecting HPV-16 DNA in plasmids, cell-lines and, both freshly collected or archival (formalin-fixed/paraffin embedded) clinical specimens. This system is therefore suitable for epidemiological studies to identify individuals infected with HPV-16 DNA in episomal form who may be at increased risk of developing anogenital carcinomas.     

烧结法赤泥沉降中新型絮凝剂的开发与应用

在烧结法生产过程中赤泥的沉降分离是一个非常重要的环节,本文通过对絮凝剂在沉降过程中的反应机理的分析、研究,以及对PNA-1与现有絮凝剂(A—2000)的试验对照,选取适应烧结法生产的新型高效絮凝剂,以改善赤泥的沉降性能,从而达到提高设备生产效率和降低生产成本的目的。     

Development of a new method for the analysis of sphinganine and sphingosine in urine and tissues

The fumonisins are inhibitors of de novo sphingolipid biosynthesis in vitro and in vivo and thus possibly interfere with the regulation of cell growth, differentiation, and neoplastic transformation. In addition, the ratio of free sphinganine (Sa) to free sphingosine (So) has been proposed as a marker of exposure for animals or humans consuming feed or food contaminated by these toxins. A method to analyze these sphingolipid bases has been proposed [Merrill et al., 1988: Anal Biochem 171:373-381; Riley et al., 1994a: JAOAC 77:533-540] but involves numerous steps and consequently is not ideally suited to the analysis of large numbers of samples, as is often required in epidemiological studies. A new method was therefore developed for the analysis of the Sa/So ratio in tissues as well as human and rat urine. Briefly, the method involves isolation of exfoliated cells from as little as 0.5 ml of rat urine or 2 ml of human urine followed by a rapid and efficient extraction of sphingolipid bases in ethyl acetate, an optimized derivatization step with o-phthaldialdehyde and a high-pressure liquid chromatography separation on a 250 mm x 4.6 mm. 5 microns Kromasil C18 column, with a 4-step phosphate buffer/methanol gradient. Fluorescence was monitored at 340 nm excitation, 455 nm emission, and retention times for So, Sa, and C-20 Sa were about 11, 14, and 22 min, respectively. The method was adapted to tissue analysis by partially digesting approximately 30 mg tissue with trypsin to permit isolation of a cell pellet before extraction of the sphingolipids as described above. The method was applied to the analysis of So and Sa in urines and tissues of fumonisin B1 (FB1) treated and untreated male BDIV rats. The Sa/So ratio in urine of untreated rats varied from 0.1 to 0.7, and for treated rats (between 1-5 mg FB1/kg body weight daily by gavage), the ratio was between 1.2-10. In kidney, the ratio was 0.1 in control rats and varied from 4 to 10.3 in treated rats. In human urine, measurements could easily be made in 2 ml of urine in females, but in males much larger volumes were required due to the low levels of sphingolipid bases.     

Selective amplification via biotin- and restriction-mediated enrichment (SABRE), a novel selective amplification procedure for detection of differentially expressed mRNAs

We present a novel subtractive enrichment protocol for the identification of differentially expressed mRNA species. This procedure, called SABRE (selective amplification via biotin- and restriction-mediated enrichment), uses selective streptavidin-biotin affinity and restriction enzyme site reconstitution to enrich for cDNA species more abundant in one population than in another. Analysis of liver cDNA from a mouse strain expressing the neomycin resistance gene demonstrated that this procedure is capable of identifying species present in one population but absent from another. Furthermore, experiments to identify genes with circadian expression patterns in mouse liver demonstrated that SABRE is capable of detecting even modest 2- to 10-fold differences in accumulation of moderately rare mRNA species, representing as little as 0.03% of total mRNA. These experiments identified the gene encoding coumarin 7-hydroxylase as displaying circadian expression in mouse liver.     

Psychotherapy outcome research: An application of a new method for evaluating research methodology.

Reviewed 142 psychotherapy outcome studies published in 21 journals between 1978 and 1985, focusing primarily on psychotherapy as a treatment of emotional problems, but including articles regarding the use of psychotherapy as a treatment for other problems (e.g, marital discord). Of concern was whether the methodological rigor of published psychotherapy outcome studies had improved since 1978: Results fail to indicate significant overall improvement or deterioration. (PsycINFO Database Record (c) 2010 APA, all rights reserved)