纳米Au修饰电化学传感器检测沙林机理的研究

采用Raman光谱和循环伏安法研究了纳米A以u电极检测沙林的电化学反应机理。研究发现,以Fe“作为检测沙林分子中F的探针离子、纳米Au修饰的Au电极电化学传感器检测液相中的沙林,可同时检测到沙林分子中F和P=0基团,实验结果具备特征性。     

交流电沉积制备纳米枝晶拉曼基底

使用交流电沉积方法在预定的微区域内制备了纳米枝晶表面增强拉曼散射基底。通过调节交流和偏置直流电压可以控制基底的形貌、位置以及生长方向。从电化学过程、液电耦合场2个角度对拉曼基底的生长机理进行了阐述。激光共焦显微拉曼实验进一步表明：使用四巯基吡啶作为指针分子,该基底具有良好的拉曼活性。     

一种新型包埋染料的核壳纳米颗粒表面增强共振拉曼探针的研制及其在免疫分析中的应用

应用于生物分析的纳米探针主要包括:等离子体共振颗粒探针,量子点、荧光探针和拉曼探针等等.拉曼探针跟其它纳米颗粒探针相比,其重要的优点是它能提供丰富的分子结构信息,从而很大程度上避免了光谱重叠,有可能研制出各种不同的拉曼探针.特别值得注意的是,基于表面增强与共振增强相结合的纳米拉曼探针,能超灵敏地、高特异性地检测生物分子.该文采用了一种新型的合成方法,成功地制备了以纳米金为核,二氧化硅为外壳,且其中包埋有染料的金/二氧化硅核壳纳米颗粒,并把它作为一种表面增强共振拉曼(SERRS)探针用于免疫分析.     

纳米Au修饰电化学传感器直接检测液相中沙林的研究

采用电势阶跃法,在Au电极表面修饰纳米Au,制备出的电化学传感器采用循环伏安法直接检测液相中的沙林,探讨了检测沙林的电化学反应机理.研究发现,检测沙林中,在1.4～0.1 V范围循环伏安扫描之前,预先进行2.0～-2.0V循环伏安扫描,是实现电化学方法检测沙林的重要先决条件.实验表明,纳米Au修饰Au电极表面后,提高了...     

基于Ag纳米粒子的LSPR传感器装置设计与实现

设计了一种集原位芯片制备和在线检测于一体的局域表面等离子体共振(LSPR)生物传感器装置,减少了检测过程中芯片移动产生的误差,并实现了小型化、在线化、简易化的目标。在该装置上利用电沉积法制备了Ag纳米粒子的LSPR芯片,实验测试结果表明:氧化铟锡(ITO)层厚度为23±5 nm时,在-1.4 V电位,制得折射率灵敏度为246 nm/RIU的LSPR芯片。     

金纳米颗粒制备及其光学特性研究

采用晶种生长法通过改变晶种对生长溶液的体积比例、引入离子合成了棒状和星形金纳米颗粒.首先用NaBH4作为还原剂制得小粒径球形金纳米颗粒作为晶种溶液,生长溶液中引入AgNO3为辅助试剂、溴化十六烷三甲基铵(CTAB)为表面活性剂来引导合成形貌不同的颗粒.对制备的球状、棒状和星状金纳米颗粒进行了紫外-可见光谱测试和表面增强...     

纳米金刚石颗粒的衬底增强效应研究

金刚石氮空位(NV)色心由于具有发光稳定、无毒、量子特性已成为最有发展前景的生物荧光标记材料.为进一步提高生物荧光标记检测和识别的分辨率,结合表面荧光增强效应,提出了结合Si,SiO2,金属三种不同折射率的材料来实现对NV色心荧光信号的增强特性.测试结果表明:金刚石结构与金属材料相结合后荧光增强最大达到17.71倍,进一步表明荧光增强机理,为提高生物荧光标记检测和识别灵敏度与分辨率提供了研究基础.     

基于光纤传感的表面纳米修饰技术实验研究

针对光纤传感和纳米技术的广泛应用,分析了光纤表面纳米修饰技术的原理与过程.依据光纤表面纳米修饰技术的原理,对实验光纤进行了表面修饰的实验研究,并设计搭建了光纤表面修饰后的光测量的系统,进行了光谱测试.经过测试,表面纳米修饰后普通的光纤转变为敏感光纤,进一步扩大光纤传感技术的应用范围.     

Development of silver nanorod array based fiber optic probes for SERS detection

The development of intrinsic SERS fiber optic sensors, i.e., fiber optical sensors that also serve as SERS active platforms, is challenging in that an easy, robust method that integrates the SERS active platform with fiber optics is still largely missing. There is a trade-off between implementing optimal morphology of SERS active nanostructures for best enhancement effect and preserving optical transparency that allows maximum transmission of the excitation radiation and the detected signals. In the present work, highly sensitive and reproducible silver nanorod arrays (AgNRs) have been integrated to a fiber optic probe for SERS detection. The films underlying the AgNR coating have been tailored to allow maximum light transmission while maintaining optimal SERS activity. The intense spectral background from the probe fiber core is largely eliminated by using a GRIN lens to produce a tight focus of the incident radiation at the AgNR coating. The performance of the AgNR fiber optic probes has been evaluated in a forward scattering optical configuration using BPE and adenine. The low detection limit of BPE and adenine is 10⁻⁷ M. Reproducibility and sampling methods are also discussed.     

基于氧化石墨烯/金纳米粒子放大电信号的二茂铁标记型溶菌酶适体传感器

设计了一种简单的信号衰减型电化学溶菌酶适体传感器。以氧化石墨烯修饰的玻碳电极为基底（GO/GCE）,通过循环伏安法电沉积金纳米粒子在电极表面,得到AuNPs/GO/GCE。将3’端修饰巯基的单链DNA通过金硫键共价连接于电极上,而在与前者可部分杂交的适体探针链5’端标记上二茂铁甲酸。当目标物溶菌酶与适体探针链发生特异性结合,通过示差脉冲伏安法检测到二茂铁甲酸还原峰电流相应的改变,从而实现对溶菌酶的识别及定量检测。结果表明,该传感器对溶菌酶具有较好的选择性和灵敏度,对于溶菌酶的检测范围为1.08×10-11～1.08×10-8mol/L,检出限达到6.02×10-12mol/L。     

Analysis for scattering of conductive objects covered with anisotropic magnetized plasma by trapezoidal recursive convolution finite‐difference time‐domain method

The finite‐difference time‐domain method (FDTD) is extended to three‐dimensional (3D) anisotropic magnetized plasma based on the trapezoidal recursive convolution (TRC) technology. The TRC technique requires single convolution integral in the formulation as in the recursive convolution (RC) method, while maintaining the accuracy comparable to the piecewise linear recursive convolution (PLRC) method with two convolution integrals. In this article, the numerical results indicate that the TRC‐FDTD method not only improves accuracy over the RC‐FDTD with the same computational efficiency but also spends less computational time than the PLRC‐FDTD based on the same accuracy. The 3D TRC‐FDTD formula is provided and the bistatic radar scattering sections of conductive targets covered with anisotropic magnetized plasma are calculated. The results show that magnetized plasma cover layer can greatly reduce echo energy of radar targets, and the anisotropic magnetized plasma cover has better absorption effect than nonmagnetized. © 2010 Wiley Periodicals, Inc. Int J RF and Microwave CAE, 2010.     

Alpha-1-fetoprotein antibody functionalized Au nanoparticles: Catalytic labels for the electrochemical detection of α-1-fetoprotein based on TiO2 nanoparticles synthesized with ionic liquid

A novel strategy for the preparation of amperometric immunosensor for rapid determination of α-1-fetoprotein (AFP) in human serum has been developed. TiO₂ nanoparticles (NPs) were prepared by solvothermal reaction using TiCl₄ as raw materials and the mixture of ionic liquids and doubly distilled water as solvent. α-1-fetoprotein antibody (AFP Ab) was mixed with TiO₂ NPs/chitsotan (CHIT) solution and immobilized onto the surface of a glassy carbon electrode. AFP (Ab) functionalized Au NPs were used as catalytic labels for the amperometric detection of AFP by means of the electrocatalyzed reduction of Au NPs to H₂O₂. The electrochemical behavior of the immunosensor was studied. Other experimental conditions such as pH, immunoreactions temperature and time were also studied. The prepared immunosensor offers an excellent amperometric response for AFP ranging from 1.0 to 160.0 ng/mL with a detection limit of 0.1 ng/mL. The result shows that the immunosensor displays rapid response, high sensitivity, good reproducibility and favorable stability.     

Web服务业务流程规范的比较研究

随着XML和WebService技术的逐步成熟,流程层集成成为主流的企业应用集成(EAI)方式,而Web服务业务流程规范是流程层集成的基础。因此该文对典型的Web服务业务流程规范进行了介绍、比较和分析,并讨论了现有Web服务业务流程规范存在的主要问题以及今后的发展趋势。     

企业专利资源管理流程本体建模研究

针对企业专利资源管理流程中语义一致性和自动化程度不高的问题,将语义本体引入到专利管理工作流的描述中。利用IDEF5图表语言构建了企业专利资源管理流程本体,在此基础上实现了对企业专利价值评估流程的可视化描述,并结合OWL语言对流程本体进行了形式化定义,该方法提供了对相关术语和词汇的共同一致的理解,可增强流程知识的语义互操作、重用和共享,从而提高企业专利资源管理的效率。     

面向故障间格兰杰因果发现的霍克斯过程研究

现有因果关系建模方法应用于故障事件序列时,难以有效引入因果先验,使得算法结果过于稠密,同时在稀疏、时间精度低的数据上因果关系可靠性较差。将不同故障类型事件的因果关系建模为基于霍克斯过程的格兰杰因果关系,提出一种面向故障序列的格兰杰因果发现的霍克斯过程模型。将霍克斯过程拓展到离散时间域,解决低时间精度数据的建模问题,并通过构造基于贝叶斯信息准则的目标函数,保证因果结构稀疏性,进而利用基于EM算法与爬山法的迭代优化算法引入因果先验,提高模型的可靠性。实验结果表明,该方法在由不同参数生成的模拟数据上均表现突出,且在两个通信网络的真实数据集中,F1评分相比ADM4、MLE-SGL、TSSO和PCMCI算法提升15.18%以上。而通过引入根因标注和因果依赖性先验,算法的F1评分进一步提升22.43%以上,验证了引入先验的有效性。