Physical, chemical, histological and palatability characteristics of muscles from three breed-types of cattle at different times-on-feed

Forty-five steers (9-12 months of age) of Angus (n =15), Brahman (n = 15) and Brahman × Angus (n = 15) breed-types were fed a high-energy diet and then slaughtered after 0, 112 or 224 days of feeding. At 7 days post mortem, the M. longissimus and M. biceps femoris were removed from the left side of each carcass and steaks were obtained for determination of sensory panel ratings, Warner-Bratzler shear force, sarcomere length, collagen content and collagen solubility. Tenderness ratings of steaks from the M. longissimus and M. biceps femoris from Angus were generally higher than ratings for steaks from Brahman or Brahman × Angus steers. Steaks from Brahman × Angus received higher tenderness ratings than steaks from Brahman steers in only a few comparisons. The three breed-types of cattle responded to time-on-feed differently; Brahman cattle needed to have been fed longer than Angus cattle to produce equally tender beef. With increased time-on-feed, M. longissimus tenderness increased for all breed-types, but M. biceps femoris tenderness was not related to time-on-feed. Few significant differences were observed among breed-types and among time-on-feed periods for collagen content or collagen solubility. Tenderness differences were closely correlated with the contractile state of the muscle which, in turn, was associated with weight, subcutaneous fat thickness and temperature decline of the carcass.     

Properties of insoluble collagen from three different types of muscles.

1. Lyotropic solubilization of insoluble collagen from heart, M. longissimus dorsi and M. semimembranosus offers differently polymerized structures. In M. semimembranosus and in heart muscle the degree of polymerization is the highest, whilst in the solubilized portion of M. longissimus dorsi considerable proportions of alpha-and beta-chains can be observed. 2. All the polymeric structures can be broken down by pepsin and dithiothreitol treatment. 3. The proportion of collagen type I to III varies in different muscles. Collagen type I constitutes a considerable portion of the collagenous core in M. longissimus dorsi and in heart muscle, whilst in M. semimembranosus the predominating type is collagen type III.     

Tenderness profiles of ten muscles from F1 Bos indicus x Bos taurus and Bos taurus cattle cooked as steaks and roasts

Twenty Bos taurus (Hereford x Angus crosses) and 20 F1 Bos indicus x Bos taurus heifers of the same age, management and feeding regimen, were harvested and evaluated at 2 days postmortem for carcass and meat traits. Ten muscles were obtained from the right sides and aged until 10 days postmortem. Bos indicus carcasses were lighter, had less fat cover, smaller ribeyes, and less intramuscular lipid (all p ≤ 0.05). Bos taurus longissimus lumborum, gluteus medius, triceps brachii, and semimembranosus muscles cooked as steaks and roasts had a lower Warner-Bratzler shear force (WBSF) (p ≤ 0.05) than those from Bos indicus. Bos taurus deep pectoral and semitendinosus muscles cooked as roasts had a lower WBSF (p < 0.05) than Bos indicus. Infraspinatus, longissimus lumborum, and semitendinosus muscles were more tender (p < 0.05) as roasts than steaks, whereas the opposite was true for the deep pectoral and semimembranosus muscles. Seven of the 10 muscles had lower WBSF (p ≤ 0.05) for Bos taurus when cooked as steaks, roasts or both.     

Tenderness and oxidative stability of post-mortem muscles from mature cows of various ages

Two experiments were conducted to investigate the influence of age of mature cows (2-4yr, 6-8yr, and 10-12yr cows; n=6 in each) on beef quality. In Experiment 1, Longissimus dorsi (LD) steaks were stored at 3°C for 0-10d. Steaks from more mature cows had an increased (P<0.05) Warner-Bratzler shear force (WBSF) and a slower troponin-T post-mortem degradation. Storage reduced WBSF in all steak samples regardless of animal age. In Experiment 2, Semitendinosus (ST) and Semimembranosus (SM) patties were stored at 3°C for 0-7d simulating retail display. The rate of lipid oxidation during storage increased with animal age (P<0.05) and was greater in ST than in SM patties. However, myoglobin oxidation was minimally affected by animal age. Thus, advanced maturation not only intensified cow meat toughness but also lowered its oxidative stability.     

Chemical composition and cooking properties of beef forequarter muscles of Mexican cattle from different genotypes

Beef forequarter muscles biceps brachii (BRB), brachialis (BRA), complexus (COM), splenius (SPL), infraspinatus (INF), teres major (TER), rhomboideus (RHO), subscapularis (SUB), supraspinatus (SUP), triceps brachii long-head (TRB) and triceps brachii lateral-head (TRI) were obtained from Mexican beef carcasses originated from Bos indicus (Bi, n=10) or Bos taurus (Bt, n=10) young bulls. Muscles were analyzed for WBSF, cooking loss, and moisture and fat contents. INF had the lowest WBSF (37.23N) and cooking loss (31.78%) of all. RHO exhibited the lowest moisture (72.62%) and highest fat content (5.23%) of all. Bi genotype exhibited higher WBSF (53.78, 48.69N), higher cook loss (36.27, 34.32%), higher fat content (2.93, 2.35%), and lower moisture content (73.70, 75.07%) than Bt. Further research is needed to estimate the actual consumer acceptance of beef forequarter muscles and their marketing potential as individual cuts in the Mexican market.     

Conjugated Linoleic Acid (CLA) content of meat from three muscles of Massese suckling lambs slaughtered at different weights

Eighteen Massese male lambs, fed mainly maternal milk were slaughtered at 11, 14 and 17kg. Samples of Longissimus Dorsi (LD), Triceps Brachii (TB) and Semimembranosus (Sm) muscles were collected. Milk from the lamb's dams was sampled weekly. Fatty acid composition of milk and meat was determined. TB was the fattest muscle, Sm the leanest one and LD showed an intermediate value of total lipids, while the weight at slaughter did not influence total intramuscular fat content in any muscle. Although slaughter weight slightly affected overall fatty acid composition of muscles, rumenic acid and total CLA content in TB and Sm, but not in LD, significantly increased with slaughter weight. As regard milk fatty acid composition, the contents of total CLA, RA and others minor CLA isomers decreased during the first four weeks after lambing and then increased at the last control (five weeks). The animals slaughtered at a live weight of 14 and 17kg showed a greater SCD enzyme activity (estimated by product/substrate ratio) and a higher rumen activity (estimated by means of branched chain and odd chain fatty acid content in meat) than animals slaughtered at 11kg. Cis-7, trans-9 CLA content significantly increased with the slaughter age in TB and SM, while trans-7, trans-9 CLA, only increased in TB, and cis-8, cis-10 CLA, only increased in SM. Further studies are needed in order to verify weather the different behaviour of RA in LD muscle may be due to differences in muscle metabolism or fatty acid utilisation.     

Myoglobin content and beta-hydroxyacyl-CoA-dehydrogenase activity of different muscles of cattle and calf

The heart, tongue, jowl, diaphragm and tail as well as shoulder, top round, the longissimus dorsi muscle of slaughtered cattle and the diaphragms of calf were examined with respect to their myoglobin content and beta-hydroxyacyl-CoA-dehydrogenase (HADH) activity. According to Gottesmann and Hamm [1] the product of these two values, the so-called MH value, can serve as the differentiation between the diaphragm and "normal cross striated skeletal muscles". Like the diaphragm, heart, tongue and jowl of cattle show higher MH values than those of "normal beef". Muscles in the tail have the same MH values as those of normal beef muscles. There are no essential differences in the MH values of various cross-striated muscle types of cows and calves. Muscles of cattle show a slightly higher myoglobin content, whereas the HADH activity is lower than in veal.     

Factors influencing the variation in tenderness of seven major beef muscles from three Angus and Brahman breed crosses

Beef carcasses (n=30) from 3/4 Angus (A)×1/4 Brahman (B), 1/4A×3/4B, and 1/2A×1/2B F(1) crosses were used to evaluate breed type, electrical stimulation, and postmortem aging on the M. semimembranosus (SM), M. semitendinosus (ST), M. biceps femoris (BF), M. vastus lateralis (VL), M. gluteus medius (GM), M. longissimus dorsi lumborum (LD), and M. triceps brachii (TB). Shear force values decreased with increased postmortem aging to a greater extent in steaks from 3/4A×1/4B than steaks from the other breed types. Shear force values for steaks from the round (SM, ST, BF, VL) were higher than steaks from the loin (LD, GM) and chuck (TB) for both electrically stimulated and non-electrically stimulated muscles. In the LD muscle, calpastatin activities were similar among breed types. Muscle type played the greatest role in determining tenderness.     

Effects of stress by unfamiliar sounds on carcass and meat traits in bulls from three continental beef cattle breeds at different ageing times

One-hundred-and-twenty-four young bulls of three Continental beef cattle breeds were used to study the effect of pre-slaughter stress by unfamiliar sounds on carcass traits and quality characteristics of beef aged for three different periods. Stress due to unfamiliar noises had a moderately negative effect on carcass and meat quality. Carcasses from group Stressed had higher (P < 0.05) values of pH₀, pH₂₄, a*, and Warner-Bratzler shear force, and lower (P < 0.05) values of L*, b* and pressure losses than from Unstressed animals. There were significant differences (P < 0.05) among breeds in L*, a* and pressure losses. The ageing time had a significant effect on chromatic variables, WBSF and pressure losses. The cooking losses were not significantly affected by any of the three factors discussed. These results emphasize the importance of implementing appropriate management practices during pre-slaughter handling of cattle in order to reduce any possible risk factor for stress, as well as the different sensitivity of the breeds to similar stimuli.     

Physicochemical characteristics of three muscles from free-range reared Iberian pigs slaughtered at 90 kg live weight

Three muscles from free-range reared pigs with different metabolic pattern were studied. m. Masseter (M), m. Longissimus dorsi (LD) and m. Serratus ventralis (SV) the first having an oxidative metabolism and the other two an intermediate and glycolytic metabolism. m. Masseter contained the highest content of myoglobin (M: 6.65 mg/g, LD: 3.00 mg/g and SV: 3.64 mg/g; P<0.001) and exhibited the highest CIE a* (M:17.10, LD: 14.83, SV:15.34, P<0.001) and C* (M: 17.95, LD: 15.61, SV: 15.54, P<0.001) values. m. L. dorsi and S. ventralis contained a higher intramuscular fat content than m. Masseter (M: 2.26 g/100 g muscle, LD: 4.79 g/100 g muscle, SV: 3.52 g/100 g muscle, P=0.001) and lower amounts of phospholipids (M: 0.33 g/g imf, LD: 0.12 g/g imf, SV: 0.19 g/g imf; P<0.001). Fatty acid profiles from total intramuscular fat and lipid fractions, neutral lipids and polar lipids, significantly differed among muscles, there being a higher content of unsaturated fats (especially in the C18:2 and C20:4 percentages) in the m. Masseter than in the other two muscles analysed. Comparatively, muscles from 90 kg live weight Iberian pigs contained more fat and heme pigments and were redder than those from the commercial pig crosses usually produced.     

Tenderness of pork muscles as influenced by chilling rate and altered carcass suspension

Tenderness improvements in porcine muscles (M. longissimus dorsi, LD; M. semimembranosus, SM; M. biceps femoris, BF) were evaluated in a total of 72 carcasses by using combinations of three different chilling rates (fast, delayed fast, slow) and two different suspension methods (Achilles tendon, pelvic bone).

Tenderness was improved by fast chilling in LD, SM and BF by the pelvic suspension as compared to conventional suspension in the Achilles tendon (P < 0·05). The lengthening of the sarcomeres in SM and BF as produced by pelvic suspension exceeded those found in LD, without having proportional additional effect on the tenderness. While the pelvic-induced tenderization did not change significantly by delayed fast chilling, additional tenderization in BF and SM was obtained by combining pelvic suspension with slow chilling. In conventionally suspended sides, tenderness was unaffected by delayed fast chilling—with slow chilling, however, improvements were observed in LD and SM to a similar extent as obtained by the pelvic suspension. In the LD muscle, the tenderizing effect produced by treatments was largest in muscles having pH values 45 min post stunning above 6·1 (P < 0·05).     

Tenderness enhancement of beef from Bos indicus and Bos taurus cattle following electrical stimulation

High voltage electrical stimulation (1130 V peak, 14.28 bidirectional half sinusoidal pulses/s) or low voltage stimulation (45 V peak, 36 alternating square wave pulses/s) was used on cattle: (1) low voltage stimulation applied for 10 or 40 s with fast and slow chilling or high voltage stimulation for 60 s with normal chilling, applied to 100% Bos taurus cattle, (2) low voltage stimulation (40 s) and high voltage stimulation (60 s) with normal chilling applied to mixed Bos indicus and B.taurus cattle, (3) high voltage stimulation (54 s) with normal chilling applied to B. taurus and B. indicus cattle of 0–100% B. indicus composition, and (4) high voltage stimulation (60 s) applied to 100% B. taurus and 100% B. indicus cattle. All stimulation parameters enhanced the tenderness of steaks from M. longissimus thoracis et lumborum (LTL) aged at 1 °C up to 28 days compared with non stimulated LTL. Short low voltage stimulation of 10 s was marginally more effective than no stimulation and longer durations of 40 s were very effective and high voltage stimulation was most effective.     

Comparative lipid composition of porcine muscles at different anatomical locations

The influence of anatomical location on the intramuscular lipids was studied in Large White pigs. The amount of total, neutral and polar lipids and the composition of fatty acids of the above three fractions were determined for Longissimus dorsi (LD) at two points and Transversus abdominis (TA) and Biceps femoris (BF) taken from the two half carcasses of an animal. The symmetrical samples from the same animal had identical intramuscular lipid characteristics. The three muscles did not show significant differences in their total (2·8 to 2·4g/100g) and neutral (2·1 to 1·4g/100g) lipid contents. The amount of polar lipids was characteristic of every muscle (0·65 g, 0·84 g and 0·97 g per 100 g, respectively, in LD, BF and TA). The fatty acid composition of neutral lipids was influenced by the type of muscle. Polar lipid had similar fatty acid composition in the three muscles. The intramuscular lipids contained appreciable amounts of essential fatty acids of the family n-6; interestingly, a part of which (25% of EFA) was the long chain fatty acid (C20 and C22) located in the polar lipids.     

Carcass characteristics and composition of Brahman, angus and Brahman x Angus steers fed for different times-on-feed

Twenty-five steers of each of three breedtypes (Angus, Brahman and F(1) Brahman x Angus) were sorted by frame size and muscle thickness, assigned to groups (five steers of each breedtype) to be fed for 0, 56, 112, 168 or 224 days, slaughtered and compared for various carcass traits. Steers of each breedtype had similar dressing percentages. Carcasses from all three breedtypes merited similar USDA quality and yield grades; breedtypes differences in quality grade were slight. Differences were found in the fat deposition patterns exhibited by the three breedtypes. Brahman steers tended to deposit more of their total fat as subcutaneous fat early in the feeding period. Angus steers had more (P < 0·05) seam fat as a percentage of carcass weight at all five feeding periods and more (P < 0·05) kidney, pelvic and heart fat at two of the five feeding periods than Brahman steers. Brahman steers had a higher percentage of their separable lean in the muscles of the round than did steers of the other breedtypes.     

The actin content of different muscles from beef and pork

In the literature 3-methylhistidine (3-MeHis) is mentioned as a parameter for the determination of meat content. Because of the variable molar ratio of 3-MeHis in the myofibrillar protein myosin, this indicator cannot be used without some restriction. However, the content of 3-MeHis in the myofibrillar protein actin is constant. Moreover, actin is relatively heat-stable in comparison with other muscle proteins. These facts made actin an interesting parameter for determination of the meat (protein) content of heated and raw meat products.

Fast protein liquid chromatography (FPLC) was used to separate from a meat extract an actin-containing fraction without myosin. The actin content of the meat was calculated from its content in the fraction. The latter was determined by means of a 3-MeHis method.

Several muscles of the beef and pig carcass were examined for their actin contents. Generally, the actin values were comparable with the data reported in the literature.

The influence of heat treatment was also studied on one type of beef muscle. The determined actin content was not affected up to a temperature of 85°C.     

Effect of pelvic suspension on three major leg muscles in the pig carcass and implications for ham manufacture

Thirty-two female pigs were slaughtered and their left sides suspended conventionally by the Achilles tendon whilst right sides were suspended from the pelvic bone. The first 16 pigs were used to measure suspension effects on the characteristics of three major leg muscles (m. gluteobiceps, m. semimembranosus and m. rectus femoris) and to assess their suitability for ham manufacture. The remaining pigs were used for the production of hams, made from tunnel- boned whole legs injected to a target 10% weight gain, then tumbled and cooked. Compared with conventional hanging, pelvic suspension resulted in significantly (P<0.01) lower weight loss from the sides during chilling (2.8 and 2.5%). There were major changes in the shape of all three muscles, with pelvic suspension producing marked increases in the width of the m. gluteobiceps (24%) and length of the m. semimembranosus (34%), and a small reduction in the length of the m. rectus femoris (2%). It also resulted in the two former muscles having a relatively higher brine retention, lower drip losses after storage and lower instrumental texture values (Volodkevitch shear force). Cooking loss was reduced in the pelvic-suspended m. semimembranosus. Similarly, whole gammon hams made from pelvic-suspended sides had greater injection gains than hams from conventionally-hung sides (13.6 and 12.3%), with lower cooking losses (16.7 and 19.0%). Instrumental texture values (punch and die peak force) made on the individual muscles in 3-mm thick slices were lower for the m. gluteobiceps (5914 and 9085 g) and m. semimembranosus (6163 and 7600 g) as a result of pelvic suspension. There were minor but significant effects on some colour parameters which probably have little commercial impact. The results demonstrate the advantages of pelvic suspension in terms of brine uptake and retention, and hence increased yield, as well as reduced toughness, for at least two of the major leg muscles, m. gluteobiceps and m. semimembranosus, with no disadvantage for the m. rectus femoris. Together, these three muscles constitute about half of the trimmed leg weight.     

Protein extractability and thermal gel formability of myofibrils isolated from skeletal and cardiac muscles at different post-mortem periods

The effects of the post-mortem ageing period on the extractability of myofibrillar proteins from pork cardiac and rabbit skeletal muscles under various conditions of pH and ionic strength were studied with particular reference to the changes in the solubility of individual myofibrillar proteins and their denaturation characteristics. The ultimate influence of these changes on the heat-induced gel forming ability of myofibrils isolated from cardiac and skeletal muscles at different post-mortem stages was also investigated. Results showed that pork cardiac myofibrils always exhibited lower solubility than those from rabbit skeletal muscles under identical conditions of pH, ionic strength and temperature. SDS-PAGE profiles indicated several quantitative differences in the relative proportion of individual protein species present in cardiac and skeletal myofibrils. The solubility of various proteins present in myofibrils was also affected differently on heating in 0-1 and 0-6 _M NaCl solution at various pH values. Thermal denaturation of cardiac myofibrils occurred at about 10°C higher than that of skeletal myofibrils as revealed by differential scanning calorimetry. Cardiac myofibrils formed much weaker heat-induced gels than those produced by skeletal myofibrils under identical conditions of temperature, pH, ionic strength and protein content.     

Repeatability of anogenital distance measurements from birth to maturity and at different physiological states in female Holstein cattle

The inverse association between anogenital distance (AGD; the distance from the center of the anus to the base of the clitoris) and fertility, its moderate heritability, and high variability reported in dairy cattle make AGD a promising candidate for further exploration as a reproductive phenotype. In addition to heritability, repeatability (i.e., consistency in measurements taken at different time points) is important for a reproductive phenotype to be considered useful in genetic selection. Therefore, our primary objective was to determine the repeatability of AGD from birth to breeding age (≈16 mo) in Holstein heifer calves, and during different stages of the estrous cycle, gestation, and lactation in Holstein cows. We also determined the associations among AGD, height (at the hip), and body weight (BW) at birth. In calves (n = 48), we recorded BW (kg) and height (cm) at birth and measured AGD (mm) at approximately 0, 2, 6, 9, 12, and 16 mo of age. In cows, AGD was measured at different stages of the estrous cycle (proestrus, estrus, metestrus and diestrus; n = 20), gestation (30, 90, 180, and 270 d; n = 78), and lactation (30–300 d in milk in 30-d increments; n = 30). Calf height and BW at birth had a weak positive association with AGD at birth. The AGD increased linearly from birth to breeding age, but there was no association between the AGD at birth and at breeding age in heifers. Although any 2 consecutive AGD measurements were correlated, 6 mo was the earliest age at which AGD was moderately correlated (r = 0.41) with that of breeding-age heifers. The AGD was neither influenced by the different stages of estrous cycle nor lactation and remained highly repeatable (r ≥ 0.95). Although AGD measurements at 30, 90, and 180 d of gestation (126.9, 126.7, and 127.7 mm, respectively) were strongly correlated (r ≥ 0.97) with each other, AGD at 270 d of gestation (142.8 mm) differed from AGD at all earlier stages of gestation. In summary, AGD measured at birth did not reflect AGD at breeding age in heifers, but AGD measurements in cows had high repeatability at all stages of the estrous cycle, gestation, and lactation, except at 270 d of gestation. Therefore, AGD could be measured reliably at any of the aforesaid physiological states in cows due to its high repeatability, except during late gestation. The earliest gestational stage when pregnancy-associated increase in AGD occurred, however, could not be definitively established in the present study.     

The effects of various post-mortem treatments on certain physical and sensory properties of three different bovine muscles

Sixty crossbred steers with chronological ages approximating 15 months, carcass weights approximating 270 kg and subcutaneous fat thickness measurements approximating 1·2 cm, were maintained for approximately 210 days on a constantly increasing concentrate ration (13 to 75%, according to NAS nutrient requirements for beef cattle; NAS, 1976). These animals were utilized to evaluate the effects of delayed chilling, hot-boning and electrical stimulation on certain physical, histological, biochemical and sensory properties of three different muscles (Semimembranosus, SM; Longissimus dorsi, LD; and Triceps brachii, TB). Results indicated that neither delayed chilling nor electrical stimulation produced consistent meaningful alterations in any of the physical, cooking or palatability attributes evaluated. However, early post-mortem excision of muscle (hot-boning) consistently produced toughening in all of the muscles evaluated. This was observed to a much greater degree in the TB than in either the SM or LD. Based upon sarcomere length determinations, such toughening could be attributed to cold shortening only in the TB. Electrical stimulation of excised muscle was ineffective in preventing or offsetting this toughening. The delayed chilling treatment imposed (2 h at 12·5°C) was ineffective in enhancing potential effects of electrical stimulation on tenderness.