共查询到20条相似文献，搜索用时 15 毫秒
1.
Jingwen Wang Junjie Yuan Jiajia Liu Haixia Zou Lin Yang Hong Chen Xiangmeng Qu 《Small (Weinheim an der Bergstrasse, Germany)》2023,19(28):2207622
DNA self-assembly provides a “bottom-up” route to fabricating complex shapes on the nanometer scale. However, each structure needs to be designed separately and carried out by professionally trained technicians, which seriously restricts its development and application. Herein, a point-and-shoot strategy based on enzyme-assisted DNA “paper-cutting” to construct planar DNA nanostructures using the same DNA origami as the template is reported. Precisely modeling the shapes with high precision in the strategy based on each staple strand of the desired shape structure hybridizes with its nearest neighbor fragments from the long scaffold strand. As a result, some planar DNA nanostructures by one-pot annealing the long scaffold strand and selected staple strands is constructed. The point-and-shoot strategy of avoiding DNA origami staple strands’ re-designing based on different shapes breaks through the shape complexity limitation of the planar DNA nanostructures and enhances the simplicity of design and operation. Overall, the strategy's simple operability and great generality enable it to act as a candidate tool for manufacturing DNA nanostructures. 相似文献
2.
Yingxu Shang Na Li Shengbo Liu Ling Wang Zhen-Gang Wang Zhong Zhang Baoquan Ding 《Advanced materials (Deerfield Beach, Fla.)》2020,32(21):2000294
DNA origami has been widely investigated as a template for the organization of various functional elements, leading to potential applications in many fields such as biosensing, nanoelectronics, and nanophotonics. However, the synthesis of inorganic nonmetallic nanomaterials with predesigned patterns using DNA origami templates has seldom been explored. Here, a novel method is reported to site-specifically synthesize silica nanostructures with designed patterns on DNA origami templates. The molecular dynamic simulation confirms that the positively charged silica precursors have a stronger electrostatic affinity to protruding double-stranded DNA (dsDNA) than DNA origami surfaces. The work describes a novel strategy to fabricate silica nanostructures with nanoscale precision. Moreover, the site-specific silicification of DNA nanoarchitectures expands the scope of customized synthesis of inorganic nonmetallic nanomaterials. 相似文献
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Ian T. Hoffecker Sijie Chen Andreas Gdin Alessandro Bosco Ana I. Teixeira Bjrn Hgberg 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(1)
Self‐assembled DNA origami nanostructures have a high degree of programmable spatial control that enables nanoscale molecular manipulations. A surface‐tethered, flexible DNA nanomesh is reported herein which spontaneously undergoes sharp, dynamic conformational transitions under physiological conditions. The transitions occur between two major macrostates: a spread state dominated by the interaction between the DNA nanomesh and the BSA/streptavidin surface and a surface‐avoiding contracted state. Due to a slow rate of stochastic transition events on the order of tens of minutes, the dynamic conformations of individual structures can be detected in situ with DNA PAINT microscopy. Time series localization data with automated imaging processing to track the dynamically changing radial distribution of structural markers are combined. Conformational distributions of tethered structures in buffers with elevated pH exhibit a calcium‐dependent domination of the spread state. This is likely due to electrostatic interactions between the structures and immobilized surface proteins (BSA and streptavidin). An interaction is observed in solution under similar buffer conditions with dynamic light scattering. Exchanging between solutions that promote one or the other state leads to in situ sample‐wide transitions between the states. The technique herein can be a useful tool for dynamic control and observation of nanoscale interactions and spatial relationships. 相似文献
4.
Yoones Kabiri Adithya N. Ananth Jaco van der Torre Allard Katan Jin‐Yong Hong Sairam Malladi Jing Kong Henny Zandbergen Cees Dekker 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(31)
While graphene may appear to be the ultimate support membrane for transmission electron microscopy (TEM) imaging of DNA nanostructures, very little is known if it poses an advantage over conventional carbon supports in terms of resolution and contrast. Microscopic investigations are carried out on DNA origami nanoplates that are supported onto freestanding graphene, using advanced TEM techniques, including a new dark‐field technique that is recently developed in our lab. TEM images of stained and unstained DNA origami are presented with high contrast on both graphene and amorphous carbon membranes. On graphene, the images of the origami plates show severe unwanted distortions, where the rectangular shape of the nanoplates is significantly distorted. From a number of comparative control experiments, it is demonstrated that neither staining agents, nor screening ions, nor the level of electron‐beam irradiation cause this distortion. Instead, it is suggested that origami nanoplates are distorted due to hydrophobic interaction of the DNA bases with graphene upon adsorption of the DNA origami nanoplates. 相似文献
5.
Liquid Crystals: Alignment and Graphene‐Assisted Decoration of Lyotropic Chromonic Liquid Crystals Containing DNA Origami Nanostructures (Small 12/2016) 
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下载免费PDF全文 Kevin Martens Timon Funck Susanne Kempter Eva‐Maria Roller Tim Liedl Benno M. Blaschke Peter Knecht José Antonio Garrido Bingru Zhang Heinz Kitzerow 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(12):1542-1542
6.
Yang Xin Charlotte Kielar Siqi Zhu Christoph Sikeler Xiaodan Xu Christin Mser Guido Grundmeier Tim Liedl Amelie Heuer‐Jungemann David M. Smith Adrian Keller 《Small (Weinheim an der Bergstrasse, Germany)》2020,16(13)
Although DNA origami nanostructures have found their way into numerous fields of fundamental and applied research, they often suffer from rather limited stability when subjected to environments that differ from the employed assembly conditions, that is, suspended in Mg2+‐containing buffer at moderate temperatures. Here, means for efficient cryopreservation of 2D and 3D DNA origami nanostructures and, in particular, the effect of repeated freezing and thawing cycles are investigated. It is found that, while the 2D DNA origami nanostructures maintain their structural integrity over at least 32 freeze–thaw cycles, ice crystal formation makes the DNA origami gradually more sensitive toward harsh sample treatment conditions. Whereas no freeze damage could be detected in 3D DNA origami nanostructures subjected to 32 freeze–thaw cycles, 1000 freeze–thaw cycles result in significant fragmentation. The cryoprotectants glycerol and trehalose are found to efficiently protect the DNA origami nanostructures against freeze damage at concentrations between 0.2 × 10?3 and 200 × 10?3 m and without any negative effects on DNA origami shape. This work thus provides a basis for the long‐term storage of DNA origami nanostructures, which is an important prerequisite for various technological and medical applications. 相似文献
7.
Kevin Martens Timon Funck Susanne Kempter Eva‐Maria Roller Tim Liedl Benno M. Blaschke Peter Knecht José Antonio Garrido Bingru Zhang Heinz Kitzerow 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(12):1658-1666
Composites of DNA origami nanostructures dispersed in a lyotropic chromonic liquid crystal are studied by polarizing optical microscopy. The homogeneous aqueous dispersions can be uniformly aligned by confinement between two glass substrates, either parallel to the substrates owing to uniaxial rubbing or perpendicular to the substrates using ozonized graphene layers. These opportunities of uniform alignment may pave the way for tailored anisometric plasmonic DNA nanostructures to photonic materials. In addition, a decorated texture with nonuniform orientation is observed on substrates coated with pristine graphene. When the water is allowed to evaporate slowly, microscopic crystal needles appear, which are aligned along the local orientation of the director. This decoration method can be used for studying the local orientational order and the defects in chromonic liquid crystals. 相似文献
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Ulrich Kemper Jingjing Ye David Poppitz Roger Gläser Ralf Seidel 《Small (Weinheim an der Bergstrasse, Germany)》2023,19(26):2206438
DNA origami molds allow a shape-controlled growth of metallic nanoparticles. So far, this approach is limited to gold and silver. Here, the fabrication of linear palladium nanostructures with controlled lengths and patterns is demonstrated. To obtain nucleation centers for a seeded growth, a synthesis procedure of palladium nanoparticles (PdNPs) using Bis(p-sulfonatophenyl)phenylphosphine (BSPP) both as reductant and stabilizer is developed to establish an efficient functionalization protocol of the particles with single-stranded DNA. Attaching the functionalized particles to complementary DNA strands inside DNA mold cavities supports subsequently a highly specific seeded palladium deposition. This provides rod-like PdNPs with diameters of 20–35 nm of grainy morphology. Using an annealing procedure and a post-reduction step with hydrogen, homogeneous palladium nanostructures can be obtained. With the adaptation of the procedure to palladium the capabilities of the mold-based tool-box are expanded. In the future, this may allow a facile adaptation of the mold approach to less noble metals including magnetic materials such as Ni and Co. 相似文献
10.
Chalmers Chau;Gayathri Mohanan;Iain Macaulay;Paolo Actis;Christoph Wälti; 《Small (Weinheim an der Bergstrasse, Germany)》2024,20(20):2308776
DNA origami synthesis is a well-established technique with wide-ranging applications. In most cases, the synthesized origami must be purified to remove excess materials such as DNA oligos and other functional molecules. While several purification techniques are routinely used, all have limitations, and cannot be integrated with robotic systems. Here the use of solid-phase reversible immobilization (SPRI) beads as a scalable, high-throughput, and automatable method to purify DNA origami is demonstrated. Not only can this method remove unreacted oligos and biomolecules with yields comparable to existing methods while maintaining the high structural integrity of the origami, but it can also be integrated into an automated workflow to purify simultaneously large numbers and quantities of samples. It is envisioned that the SPRI beads purification method will improve the scalability of DNA nanostructures synthesis both for research and commercial applications. 相似文献
11.
Zhong Chen Xiang Lan Qiangbin Wang 《Small (Weinheim an der Bergstrasse, Germany)》2013,9(21):3724-3724
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Zhong Chen Xiang Lan Qiangbin Wang 《Small (Weinheim an der Bergstrasse, Germany)》2013,9(21):3567-3571
13.
《Advanced Materials Interfaces》2018,5(14)
Self‐assembly of designed nanostructures on a lipid membrane offers a powerful approach for enhancing the functionality of its surface. Here, the environment‐dependent assembly of blunt‐ended DNA origami structures on a phase‐separated lipid‐bilayer membrane consisting of liquid‐disordered (Ld) and solid‐ordered (So) phases is reported. Fluorescence microscopy and atomic force microscopy (AFM) imaging reveal that DNA origami structures preferentially bind to the So phase. At higher concentrations, blunt‐ended origamis form 2D lattices on the Ld phase through surface‐mediated self‐assembly. In contrast, those adsorbed on the So phase are less mobile and pack into aggregates. However, this situation is changed by adding NaCl. High‐speed AFM imaging reveals the mechanism underlying the NaCl‐induced changes, wherein the lattices on the Ld phase desorb from the surface while the packed aggregates on the So phase reorganize into the lattices. These results indicate that the formation of 2D lattices depends on the phase of the lipids; however, it can be tuned by ionic conditions, enabling us to select the domain on which the lattice is formed. The present results may guide the creation of membrane‐supported DNA nanostructures that self‐assemble into functional states in a lipid phase or ion‐responsive manner. 相似文献
14.
Miran Liber Toma E. Tomov Roman Tsukanov Yaron Berger Mary Popov Dinesh C. Khara Eyal Nir 《Small (Weinheim an der Bergstrasse, Germany)》2018,14(23)
Organizing DNA origami building blocks into higher order structures is essential for fabrication of large structurally and functionally diverse devices and molecular machines. Unfortunately, the yields of origami building block attachment reactions are typically not sufficient to allow programed assembly of DNA devices made from more than a few origami building blocks. To investigate possible reasons for these low yields, a detailed single‐molecule fluorescence study of the dynamics of rectangular origami dimerization and origami dimer dissociation reactions is conducted. Reactions kinetics and yields are investigated at different origami and ion concentrations, for different ion types, for different lengths of bridging strands, and for the “sticky end” and “weaving welding” attachment techniques. Dimerization yields are never higher than 86%, which is typical for such systems. Analysis of the dynamic data shows that the low yield cannot be explained by thermodynamic instability or structural imperfections of the origami constructs. Atomic force microscopy and gel electrophoresis evidence reveal self‐dimerization of the origami monomers, likely via blunt‐end interactions made possible by the presence of bridging strands. It is suggested that this mechanism is the major factor that inhibits correct dimerization and means to overcome it are discussed. 相似文献
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Breveruos Sheheade Miran Liber Mary Popov Yaron Berger Dinesh C. Khara Jürgen Jopp Eyal Nir 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(51)
Efficient fabrication of structurally and functionally diverse nanomolecular devices and machines by organizing separately prepared DNA origami building blocks into a larger structure is limited by origami attachment yields. A general method that enables attachment of origami building blocks using ‘sticky ends' at very high yields is demonstrated. Two different rectangular origami monomers are purified using agarose gel electrophoresis conducted in solute containing 100 × 10?3 m NaCl, a treatment that facilitates the dissociation of most of the incorrectly hybridized origami structures that form through blunt‐end interactions during the thermal annealing process and removes these structures as well as excess strands that otherwise interfere with the desired heterodimerization reaction. Heterodimerization yields of gel‐purified monomers are between 98.6% and 99.6%, considerably higher than that of monomers purified using the polyethylene glycol (PEG) method (88.7–96.7%). Depending on the number of PEG purification rounds, these results correspond to about 4‐ to 25‐fold reduction in the number of incorrect structures observed by atomic force microscopy. Furthermore, the analyses of the incorrect structures observed before and after the heterodimerization reactions and comparison of the purification methods provide valuable information on the reaction mechanisms that interfere with heterodimerization. 相似文献
17.
Tao Zhang Caroline Hartl Kilian Frank Amelie Heuer‐Jungemann Stefan Fischer Philipp C. Nickels Bert Nickel Tim Liedl 《Advanced materials (Deerfield Beach, Fla.)》2018,30(28)
3D crystals assembled entirely from DNA provide a route to design materials on a molecular level and to arrange guest particles in predefined lattices. This requires design schemes that provide high rigidity and sufficiently large open guest space. A DNA‐origami‐based “tensegrity triangle” structure that assembles into a 3D rhombohedral crystalline lattice with an open structure in which 90% of the volume is empty space is presented here. Site‐specific placement of gold nanoparticles within the lattice demonstrates that these crystals are spacious enough to efficiently host 20 nm particles in a cavity size of 1.83 × 105 nm3, which would also suffice to accommodate ribosome‐sized macromolecules. The accurate assembly of the DNA origami lattice itself, as well as the precise incorporation of gold particles, is validated by electron microscopy and small‐angle X‐ray scattering experiments. The results show that it is possible to create DNA building blocks that assemble into lattices with customized geometry. Site‐specific hosting of nano objects in the optically transparent DNA lattice sets the stage for metamaterial and structural biology applications. 相似文献
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Sisi Fan Dongfang Wang Ahmad Kenaan Jin Cheng Daxiang Cui Jie Song 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(26)
Structural deoxyribonucleic acid (DNA) nanotechnology offers a robust platform for diverse nanoscale shapes that can be used in various applications. Among a wide variety of DNA assembly strategies, DNA origami is the most robust one in constructing custom nanoshapes and exquisite patterns. In this account, the static structural and functional patterns assembled on DNA origami are reviewed, as well as the reconfigurable assembled architectures regulated through dynamic DNA nanotechnology. The fast progress of dynamic DNA origami nanotechnology facilitates the construction of reconfigurable patterns, which can further be used in many applications such as optical/plasmonic sensors, nanophotonic devices, and nanorobotics for numerous different tasks. 相似文献