Energy recovery from grass of urban roadside verges by anaerobic digestion and combustion after pre-processing

Grass from urban roadside verges is a potential, though widely unused, resource for bioenergy recovery. Two possible bioenergy recovery techniques were tested, i.e. i) direct anaerobic digestion of the whole parent material and ii) the “integrated generation of solid fuel and biogas from biomass” (IFBB) procedure, which divides biomass into a press fluid and a press cake by mashing and mechanical dewatering. Biomass yield, chemical composition and canopy height of biomass, contribution of functional groups, fermentation characteristics of silage and press fluids, as well as characteristics of the produced solid fuel was investigated, applying a 4-cut management for anaerobic digestion, a 2-cut management for IFBB and an 8 times mulching as a reference. Mean annual biomass yield (2013 and 2014) was 3.24, 3.33 and 5.68 t dry matter ha⁻¹ for the mulching, 4-cut management and 2-cut management, respectively. Yields were higher in 2014 due to more favourable weather conditions. Fibre concentration was higher in material of the 2-cut management than in the 4-cut management, however, methane yield of the corresponding silages was the same. Highest methane yield was gained from press fluids with 292 l_N kg⁻¹ volatile solids. The press cake had a lower heating value of 16 MJ kg⁻¹ dry matter and a K₂O/CaO index of 0.51–0.88. Gross energy output was 26.4 GJ ha⁻¹ for anaerobic digestion and 84.4 GJ ha⁻¹ for IFBB. Thus, an altered roadside verge management with reduced cutting frequency might allow a significant energy recovery and improved ecosystem services, i.e. increased biodiversity.     

Growth,yield, fiber content and lodging resistance in eight varieties of Cenchrus purpureus (Schumach.) Morrone intended as energy crop

Growth, biomass yield, fiber content and lodging resistance were studied, during a six month growth period, for eight varieties of Cenchrus purpureus, intended as energy crop, in Veracruz, Mexico. Then, only yield at day 182 was assessed for two additional years. The varieties were: CT115 (CT), African Cane (AC), Taiwan (TAI), King Grass (KG), Vruckwona (VRU), Roxo (RX), OM22 (OM) and Cameroon (CAM). Local weather is warm and sub-humid, historical data for monthly average temperature and annual rainfall were 25.8 °C and 1142 mm, respectively. Height, diameter and light interception were measured monthly from day 65–185. At day 185, biomass yield and tiller density were measured. Number of lying tillers was counted to estimate lodging resistance. Cellulose and hemicellulose content were estimated in leaf and stem. No differences were found for dry matter yield or stem yield at day 185 in the first year. Regarding the next two years, TAI yielded above CT, OM or ROX. Average dry matter yield was higher in the second year than in the establishment cycle (38.6 vs 21.1 Mg ha⁻¹), but decreased in the third year (32.2 Mg ha⁻¹). In both stem and whole plant, AC and KG showed higher hemicellulose content than RX, OM or CT; while AC and VRU had higher cellulose than RX in stem, or than CT in the whole plant. Furthermore, varieties AC, KG, VRU and TAI were resistant to lodging and had a higher fiber content, so they are recommended as energetic crops.     

Anaerobic digestion of corn silage on a commercial scale: Differential utilization of its chemical constituents and characterization of the solid digestate

The utilization of different chemical constituents of corn silage during industrial-scale anaerobic digestion was determined. Corn silage together with the resulting solid digestate generated during biogas production were collected from an industrial plant during a regular operating period. Moisture, water and ethanol extractives, ash, total nitrogen, starch, cellulose, the monomeric composition of hemicellulose, acid soluble and acid insoluble lignin were measured in both corn silage and corn silage solid digestate. The relative consumption of each component of corn silage during its anaerobic digestion was estimated with reference to acid insoluble lignin. It was assumed that lignin was not digested throughout the process. Starch and large fractions of extractives and acid soluble lignin were digested. In contrast, the digestion of cellulose and particularly hemicellulose were limited (40% and 29% respectively). Of the hemicellulose monomers, xylose was the least digested (20%). The present work shows that the digestate produced by commercial corn-silage anaerobic digestion contains a notable quantity of cell wall polymers. These could potentially be used in biorefinery processes, e.g. ethanol and xylo-oligosaccharide production.     

Combined ethanol and methane production from switchgrass (Panicum virgatum L.) impregnated with lime prior to steam explosion

Pretreatments are crucial to achieve efficient conversion of lignocellulosic biomass to soluble sugars. In this light, switchgrass was subjected to 13 pretreatments including steam explosion alone (195 °C for 5, 10 and 15 min) and after impregnation with the following catalysts: Ca(OH)₂ at low (0.4%) and high (0.7%) concentration; Ca(OH)₂ at high concentration and higher temperature (205 °C for 5, 10 and 15 min); H₂SO₄ (0.2% at 195 °C for 10 min) as reference acid catalyst before steam explosion. Enzymatic hydrolysis was carried out to assess pretreatment efficiency in both solid and liquid fraction. Thereafter, in selected pretreatments the solid fraction was subjected to simultaneous saccharification and fermentation (SSF), while the liquid fraction underwent anaerobic digestion (AD). Lignin removal was lowest (12%) and highest (35%) with steam alone and 0.7% lime, respectively. In general, higher cellulose degradation and lower hemicellulose hydrolysis were observed in this study compared to others, depending on lower biomass hydration during steam explosion. Mild lime addition (0.4% at 195 °C) enhanced ethanol in SSF (+28% than steam alone), while H₂SO₄ boosted methane in AD (+110%). However, methane represented a lesser component in combined energy yield (ethanol, methane and energy content of residual solid). Mild lime addition was also shown less aggressive and secured more residual solid after SSF, resulting in higher energy yield per unit raw biomass. Decreased water consumption, avoidance of toxic compounds in downstream effluents, and post process recovery of Ca(OH)₂ as CaCO₃ represent further advantages of pretreatments involving mild lime addition before steam explosion.     

Sunflower as a biofuels crop: An analysis of lignocellulosic chemical properties

Four accessions of cultivated sunflower (Helianthus annuus) and silverleaf sunflower (Helianthus argophyllus), were each grown in three locations (Georgia, British Columbia, and Iowa) at different planting densities and phenotyped for biomass-related traits and wood biochemistry. In most environments, H. argophyllus produced significantly more biomass than H. annuus. Cell wall chemistry for a subset of plants grown in Georgia and Iowa was assessed using analytical wet chemistry methods to measure lignin and sugar content/composition. The analysis of lignin and the S/G-lignin ratios for a larger number of samples (n > 250) was also assessed by high-throughput pyrolysis Molecular Beam Mass Spectrometry. Average pyMBMS estimated lignin content (i.e., dry weight fraction) for 60 °C dried basal stem samples of H. annuus and H. argophyllus was 29.6% (range, 24.0%–34.6%) and 28.6% (range, 24.6%–33.3%), respectively when averaged across all environments. The average S/G lignin mass ratio was 1.5 (range, 1.0–2.0) for H. annuus and 1.7 (range, 1.0–2.4) in H. argophyllus. Stem samples from these two species only differed statistically for a few cell wall chemistry traits; however, accession level differences within each species were apparent. Cell wall chemistry in both species was significantly affected by both location and planting density, thus demonstrating the need to select for these traits in the environment for which the crop will be produced. Overall, these results show that cultivated sunflower and silverleaf sunflower both possess the necessary phenotypic diversity to facilitate the development of a hybrid sunflower with improved lignocellulosic biofuels traits, namely increased biomass, decreased lignin, and increased glucan.     

Environmental assessment of electricity generation from an Italian anaerobic digestion plant

A standard ISO Life Cycle Assessment study was carried out to evaluate the environmental sustainability of electricity production from an anaerobic digestion (AD) plant using a mixture of dedicated energy crops, agricultural residues and livestock effluents as input materials. The functional unit was 1 MJ of electricity. System boundaries were from cradle to grave and covered all the phases from energy crops cultivation to the production of biogas and its use in a Combined Heat and Power plant to produce electricity. Liquid and solid digestate storage and spreading on agricultural land were included. Primary data were collected from the AD plant for all the above phases. Since heat produced is used only internally, no allocation was applied in the study. As regards digestate management, CH₄ emissions were calculated from literature, whereas four literature methods were applied for calculation of nitrogen emissions with the goal to perform a sensitivity analysis on LCA results. ILCD Handbook impact assessment methodologies were used. Results show that the main hotspots are energy crops cultivation and the management of digestate, mainly because of both nitrogen and methane emissions, affecting Global Warming, Acidification, Marine and Freshwater Eutrophication. Finally, a detailed Monte Carlo analysis, was carried out to evaluate the results uncertainty. The study represents the state of the art about the environmental performance of the AD plant with the use of sensitivity and uncertainty analysis, which both improve the reliability of results, and allows drawing general conclusions on how to mitigate the environmental impacts of AD process.     

Improvement of enzymatic saccharification of Populus and switchgrass by combined pretreatment with steam and wet disk milling

To reduce the recalcitrance of lignocellulosic biomass for subsequent biological processing, we pretreated energy crop feedstocks with mild steam treatment (ST; 130 and 150 °C for 60 min) and wet disk milling (WDM). We tested two phylogenetically different, but typical energy crop feedstocks: Populus trichocarpa and switchgrass (Panicum virgatum). WDM after ST facilitated the fibrillation of both types of biomass, resulting in an increase of specific surface area, improved enzymatic saccharification yield, and decrease in cellulose crystallinity. After steam treatment at 150 °C followed by 17 cycles of WDM, enzymatic hydrolysis resulted in almost complete glucan to glucose conversion in both feedstocks.     

Simultaneous enzymatic saccharification and ABE fermentation using pretreated oil palm empty fruit bunch as substrate to produce butanol and hydrogen as biofuel

Simultaneous saccharification and acetone–ethanol–butanol (ABE) fermentation was conducted in order to reduce the number of steps involved in the conversion of lignocellulosic biomass into butanol. Enzymatic saccharification of pretreated oil palm empty fruit bunch (OPEFB) by cellulase produced 31.58 g/l of fermentable sugar. This saccharification was conducted at conditions similar to the conditions required for ABE fermentation. The simultaneous process by Clostridium acetobutylicum ATCC 824 produced 4.45 g/l of ABE with butanol concentration of 2.75 g/l. The butanol yield of 0.11 g/g and ABE yield of 0.18 g/g were obtained from this simultaneous process as compared to the two-step process (0.10 g/g of butanol yield and 0.14 g/g of ABE yield). In addition, the simultaneous process also produced higher cumulative hydrogen (282.42 ml) than to the two-step process (222.02 ml) after 96 h of fermentation time. This study suggested that the simultaneous process has the potential to be implemented for the integrated production of butanol and hydrogen from lignocellulosic biomass.     

Second-generation bioethanol of hydrothermally pretreated stover biomass from maize genotypes

Twelve maize genotypes, were agronomically evaluated and their stover hydrothermally pretreated in a temperature range of 210–225 °C to assess the effects of genotype and pretreatment severity on stover recalcitrance toward bioethanol conversion. Maize genotypes exhibited significant variation for biomass yield and all agronomic evaluated, while among all cell wall constituents measured in the unpretreated stover, only ash content showed differences among genotypes. The pretreatment severities assayed impacted most stover compositional traits, and the glucose recovered after enzymatic hydrolysis displayed a similar profile among genotypes with similar genetic background. Harsher pretreatment conditions maximized the potential cellulosic bioethanol production (208–239 L/t), while the mildest maximized the bioethanol from the hemicellulosic hydrolysates (137–175 L/t). Consequently, when both pentose and hexose sugars were considered, the total potential bioethanol produced at the lowest and highest pretreatment temperatures was similar in all genotypes (292–358 L/t), indicating that the lowest temperature (210 °C) was the optimal among all assayed. Importantly, the ranking of genotypes for bioethanol yield (L/ha) closely resembled the ranking for stover yield (t/ha), indicating that breeding for biomass yield would increase the bioethanol production per hectare regardless of the manufacturing process. Similarly, the genetic regulation of corn stover moisture is possible and relevant for efficient energy production as biomass moisture has a potential impact on stover transportation, storage and processing requirements. Overall, these results indicate that local landrace populations are important genetic resources to improve cultivated crops, and that simultaneous breeding for production of grain and stover bioethanol is possible in corn.     

Characterization,pretreatment and saccharification of spent seaweed biomass for bioethanol production using baker's yeast

Seaweeds are marine macroalgae found abundantly and viewed as potential source of phycocolloids to produce biofuel. In this study, seaweed spent biomass obtained from alginate production industry and biomass obtained after pigment extraction were found to contain a considerable amount of phycocolloids. These two spent biomasses were investigated for the production of ethanol. In this study, the red seaweed spent biomass of Gracilaria corticata var corticata showed higher content of polysaccharide (190.71 ± 30.67 mg g⁻¹ dry weight) than brown seaweed spent biomass (industrial) (136.28 ± 30.09 mg g⁻¹ dry weight). Hydrolysis of spent biomasses with different concentrations of sulfuric acid (0.1%, 0.5% and 1%) was also investigated. Brown seaweed spent biomass and red seaweed spent biomass exhibited high amount of sugar in 0.5% and 1% sulfuric acid treatment, respectively. Proximate and ultimate composition of seaweed spent biomasses were analysed for energy value. The FT-Raman spectra exhibited similar stretches for both acid hydrolysed spent biomasses with their respective standards. Ethanol produced through a fermentation process using spent hydrolysates with baker's yeast at pH 5.3 was found to be significant. The ethanol yield from brown seaweed spent biomass and red seaweed spent biomass was observed to be 0.011 g g⁻¹ and 0.02 ± 0.003 g g⁻¹ respectively, when compared with YPD (0.42 ± 0.03 g g⁻¹) and d-galactose (0.37 ± 0.04 g g⁻¹) as standard on day 4. The present study revealed the possibility of effective utilization of spent biomass from seaweed industry for ethanol production.     

Enhanced substrate degradation and methane yield with maleic acid pre-treatments in biomass crops and residues

Organic acids are envisaged as alternative catalysts to strong mineral acids, in pre-treatment of ligno-cellulosic biomass for anaerobic digestion (AD). To evaluate this hypothesis, an untreated control and four pre-treatments (25 °C for 24 h) involving two levels of maleic acid (34.8 and 69.6 kg m⁻³), alone and combined with sulphuric acid (4 kg m⁻³), were studied in three agricultural substrates: Arundo (aka giant reed), Barley straw and B133 fibre sorghum. Methane production was assessed in a batch AD assay (35 °C for 51 days) with 4 g L⁻¹ of volatile solid (VS) load. Fibre composition and structure were investigated through chemical analysis and Fourier transform infrared (FTIR) spectrometry. Arundo and B133 that were the most and least recalcitrant substrate, respectively, staged the highest and lowest increase in methane with high maleic acid: +62% over 218 cm³ g⁻¹ of VS in untreated Arundo; +36% over 284 cm³ g⁻¹ of VS in untreated B133. Barley straw showed an intermediate behaviour (+41% over 269 cm³ g⁻¹ of VS). H₂SO₄ addition to maleic acid did not improve CH₄ output. The large increase in methane yield determined by pre-treatments was reflected in the concurrent decrease of fibre (between 14 and 39% depending on fibrous component). Based on FTIR spectra, bands assigned to hemicellulose and cellulose displayed lower absorbance after pre-treatment, supporting the hypothesis of solubilisation of structural carbohydrates and change in fibre structure. Hence, maleic acid was shown a suitable catalyst to improve biodegradability of ligno-cellulosic biomass, especially in recalcitrant substrates as Arundo.     

Assessing sustainable forest biomass potential and bioenergy implications for the northern Lake States region,USA

Forestlands in the United States have tremendous potential for providing feedstocks necessary to meet emerging renewable energy standards. The Lake States region is one area recognized for its high potential of supplying forest-derived biomass; however, the long-term availability of roundwood harvests and associated residues from this region has not been fully explored. Better distribution and temporal availability estimates are needed to formulate emerging state policies regarding renewable energy development. We used a novel predictive methodology to quantify sustainable biomass availability and likely harvest levels over a 100-year period in the Lake States region. USDA Forest Inventory and Analysis estimates of timberland were combined with published growth and yield models, and historic harvest data using the Forest Age Class Change Simulator (FACCS) to generate availability estimates. Monte-Carlo simulation was used to develop probability distributions of biomass harvests and to incorporate the uncertainty of future harvest levels. Our results indicate that 11.27–15.71 Mt y⁻¹ dry roundwood could be sustainably harvested from the Lake States. Assuming 65% collection rate, 1.87–2.62 Mt y⁻¹ residue could be removed, which if substituted for coal would generate 2.12–2.99 GW h of electricity on equivalent energy basis while reducing GHG (CO₂e) emission by 1.91–2.69 Mt annually. In addition to promoting energy security and reducing GHG emissions, forest residues for energy may create additional revenues and employment opportunities in a region historically dependent on forest-based industries.     

Structural and chemical analysis of process residue from biochemical conversion of wheat straw (Triticum aestivum L.) to ethanol

Biochemical conversion of lignocellulose to fermentable carbohydrates for ethanol production is now being implemented in large-scale industrial production. Applying hydrothermal pretreatment and enzymatic hydrolysis for the conversion process, a residue containing substantial amounts of lignin will be generated. So far little is known about the composition of this lignin residue which at present is mainly incinerated for heat and power generation and not yet converted so much into more valuable products.In this study, the structural and chemical composition of the solid and liquid fractions of lignin residue from wheat straw were analysed and processing factors discussed. Roughly 70 and 15% of the solid mass fraction consisted of lignin and ash, respectively. Residual carbohydrates mostly originated from hemicellulose in the liquid fraction and from cellulose in the solid fraction. The solid fraction also contained significant amounts of protein, which is a valuable by-product when used as animal feed or when enzymes and yeast cells are separated for process recycling. Silica was the dominant constituent in the mineral fraction and except for few fragments of lignified middle lamellae most particles in the solid fraction appeared as silica coated by lignin, hampering separation of the two components before incineration or refinement of the residue.     

Considering uncertainty in the optimal energy management of renewable micro-grids including storage devices

This paper proposes a new probabilistic framework based on 2m Point Estimate Method (2m PEM) to consider the uncertainties in the optimal energy management of the Micro Girds (MGs) including different renewable power sources like Photovoltaics (PVs), Wind Turbine (WT), Micro Turbine (MT), Fuel Cell (FC) as well as storage devices. The proposed probabilistic framework requires 2m runs of the deterministic framework to consider the uncertainty of m uncertain variables in the terms of the first three moments of the relevant probability density functions. Therefore, the uncertainty regarding the load demand forecasting error, grid bid changes and WT and PV output power variations are considered concurrently. Investigating the MG problem with uncertainty in a 24 h time interval with several equality and inequality constraints requires a powerful optimization technique which could escape from the local optima as well as premature convergence. Consequently, a novel self adaptive optimization algorithm based on θ-Particle Swarm Optimization (θ-PSO) algorithm is proposed to explore the total search space globally. The θ-PSO algorithm uses the phase angle vectors to update the velocity/position of particles such that faster and more stable convergence is achieved. In addition, the proposed self adaptive modification method consists of three sub-modification methods which will let the particles choosel the modification method which best fits their current situation. The feasibility and satisfying performance of the proposed method is tested on a typical grid-connected MG as the case study.     

Changes in feedstock quality in willow chip piles created in winter from a commercial scale harvest

Storage and handling are important facets of biomass logistics because there are associated costs and biomass properties can change significantly as material proceeds through the supply chain. Thus, this aspect of biomass supply systems requires continued study. Shrub willow chips were harvested, and used to create six piles that each contained between 10 and 22 Mg (fresh biomass). Material was monitored for several months in temporary storage to assess changes in biomass quality (moisture, ash and energy content). Internal pile temperatures increased due to biological activity and conditions within a pile quickly differentiated based on location (shell, core, top, and side). Mean moisture content increased from 42 to 47% (mass fraction) between harvest and delivery of the chips, but ranged between 37 and over 60% over the next three months depending on pile location with the shell generally drier than the core. Mean ash content increased 1 to 2% points (mass fraction) between harvesting, reloading and delivery to the trial location, but became more variable during storage. Higher heating values (HHV) were stable between 18.6 and 19.0 MJ kg⁻¹ over the six months, but lower heating values (LHV) ranged between 8.6 and 11.7 MJ kg⁻¹ and mirrored changes in moisture content. There was minimal change in chip quality over two months, but quality became more variable over longer time periods. This period could be extended, and negative effects on chip quality could be mitigated, by improving storage methods, blending different types of chips, or employing pretreatments.     

The effects of physical and chemical preprocessing on the flowability of corn stover

Continuous and reliable feeding of biomass is essential for successful biofuel production. However, the challenges associated with biomass solids handling are commonly overlooked. In this study, we examine the effects of preprocessing (particle size reduction, moisture content, chemical additives, etc.) on the flow properties of corn stover. Compressibility, flow properties (interparticle friction, cohesion, unconfined yield stress, etc.), and wall friction were examined for five corn stover samples: ground, milled (dry and wet), acid impregnated, and deacetylated. The ground corn stover was found to be the least compressible and most flowable material. The water and acid impregnated stovers had similar compressibilities. Yet, the wet corn stover was less flowable than the acid impregnated sample, which displayed a flow index equivalent to the dry, milled corn stover. The deacetylated stover, on the other hand, was the most compressible and least flowable examined material. However, all of the tested stover samples had internal friction angles >30°, which could present additional feeding and handling challenges. All of the “wetted” materials (water, acid, and deacetylated) displayed reduced flowabilities (excluding the acid impregnated sample), and enhanced compressibilities and wall friction angles, indicating the potential for added handling issues; which was corroborated via theoretical hopper design calculations. All of the “wetted” corn stovers require larger theoretical hopper outlet diameters and steeper hopper walls than the examined “dry” stovers.     

Enhancement of lipid productivity in green microalgae Chlorella sp. via fast neutron irradiation

Microalgae is becoming a promising candidate for biofuel production and energetic lipid production, which urges the need to develop efficient methods for improvement of the lipid production. In this study, the neutron irradiation was employed to improve lipid production under different dosage irradiation. Effects of different dosages (No.1:8.14 × 10⁹, No.2:5.64 × 10⁸, No.4:9.42 × 10⁷ n/cm²) were compared by investigating associated algae cell growth, total lipid production, metabolization and enzyme activity. Dosages No.2 and No.4 improved the lipid content without negative influence on the growing, whereas irradiations shortened the period of biomass accumulation. The neutron irradiation in No.2 accelerated the synthesis of lipid from the carbohydrate transition process. In addition, the neutron irradiation not only increased the total lipid production by 20% but also speeded up the reaction rate to reach the maximal total lipid production in 28 days. Our results can provide a better understanding of the lipid production in microalgae Chlorella sp. by neutron irradiation, which are critical for evaluation of neutron irradiation based technologies for the sustainable and renewable biofuel production.     

Ash reduction strategies in corn stover facilitated by anatomical and size fractionation

There is growing interest internationally to produce fuels from renewable biomass resources. Inorganic components of biomass feedstocks, referred to collectively as ash, damage equipment and decrease yields in thermal conversion processes, and decrease feedstock value for biochemical conversion processes. Decreasing the ash content of feedstocks improves conversion efficiency and lowers process costs. Because physiological ash is unevenly distributed in the plant, mechanical processes can be used to separate fractions of the plant based on ash content. This study focuses on the ash separation that can be achieved by separating corn stover by particle size and anatomical fraction. Baled corn stover was hand-separated into anatomical fractions, ground to <19.1 mm, and size separated using six sieves ranging from 9.5 to 0.150 mm. Size fractions were analyzed for total ash content and ash composition. Particle size distributions observed for the anatomical fractions varied considerably. Cob particles were primarily 2.0 mm or greater, while most of the sheath and husk particles were 2.0 mm and smaller. Particles of leaves greater than 0.6 mm contained the greatest amount of total ash, ranging from approximately 8 to 13% dry weight of the total original material, while the fractions with particles smaller than 0.6 mm contained less than 2% of the total ash of the original material. Based on the overall ash content and the elemental ash, specific anatomical and size fractions can be separated to optimize the feedstocks being delivered to biofuels conversion processes and minimize the need for more expensive ash reduction treatments.     

Upgraded biofuel from residue biomass by Thermo-Catalytic Reforming and hydrodeoxygenation

Research is focused on the utilisation of waste or residue biomass for bioenergy conversion. A promising conversion technology for the production of liquid biofuels from residue biomass is a process called Thermo-Catalytic Reforming (TCR^®​) which is a combination of prior thermal treatment of the biomass at mild temperatures (intermediate pyrolysis) followed by a second catalytic treatment step at elevated temperatures (reforming). This article focuses on the conversion of TCR^® liquids from digestate as a feedstock for subsequent hydrocarbon production. The generated bio-oil showed a lower heating value of 34.0 MJ kg⁻¹ with an oxygen content of 7.0% and a water content of 2.2%. The bio-oil was hydrodeoxygenated using an industrial NiMo–Al₂O₃ catalyst at temperatures of 503 K–643 K and a pressure of 14 MPa. The hydrodeoxygenated bio-oil reached a lower heating value of 42.3 MJ kg⁻¹ with an oxygen content below 0.8 mg kg⁻¹ and water content of 30 ppm. Product yields and catalyst life give confidence that upgrading of the TCR^®​ bio-oil offers a suitable option to meet the high standards of common fuels.     

In vitro-propagated Miscanthus × giganteus plants can be a source of diversity in terms of their chemical composition

Miscanthus × giganteus is a triploid hybrid propagated vegetatively from rhizomes or in a tissue culture, so its clones are characterised by a very low genetic diversity. Giant Miscanthus is cultivated mainly for biomass used as biofuel; depending on its chemical composition, it can be used in many industries. The goal of this work was to determine whether regenerants obtained in tissue culture can be a source of new forms characterised by different biomass yield and energy values as well as by their concentration of cellulose, hemicellulose and lignin. In the study the number and height of stems as well as the dry weight, ash and water mass fraction of biomass were estimated. The study was performed on Miscanthus plants propagated from rhizomes obtained from two different locations in Poland (Zabierzów – Z and Radzików – R), and on regenerants (Iv) obtained from Z plants. The results indicated that somaclonal variation occurs in M. × giganteus cultures and that regenerants show low polymorphic variation compared to donor plants. Plants propagated in an in vitro culture showed the lowest stem height, but the highest number of stems per plant. Dry matter yield was greatest in the R group, while regenerants demonstrated the highest level of hemicellulose fraction and lowest cellulose and lignin concentration. Energy value was the highest for Z plants, but Iv plants showed energy values on the same level as those of R plants. The data suggest that in vitro-propagated plants may be a source of new Miscanthus forms.