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1.
Biofilms are complex bacterial communities have a mechanism for antibiotic resistance leading to human health problems. It remains challenging to treat and eradicate biofilms. In this work, the use of d-maltose synthesized silver nanoparticles (AgNPs) was investigated in an effort to eradicate a biofilm. AgNPs were synthesized using a modified Tollen’s method. d-maltose was used in synthesizing AgNPs with different concentrations of d-maltose (0.01, 0.05 and 0.1 M), referred to as NP1, NP2 and NP3, respectively. TEM images revealed that the particles were polygon shaped. The particle sizes were found to be 86.81?±?13.39, 54.94?±?11.63 and 31.43?±?31.76 nm depending on their sugar concentrations. UV–Vis, ATR–FTIR, and XRD patterns were employed to characterize the AgNPs. Then, these AgNPs were investigated for their anti-bacterial effects against Escherichia coli and Staphylococcus aureus. Evaluation of the minimum inhibitory concentration and minimal bactericidal concentration revealed that S. aureus was inhibited by all AgNPs and killed by NP1 and NP3, and E. coli was inhibited and killed at all AgNPs doses. Furthermore, anti-biofilm activity against these two bacteria was observed using SEM and confocal laser scanning microscopy. This sugar coated AgNPs is a promising material for use in eradication of biofilms.  相似文献   

2.
Silver nanoparticles (AgNPs) were successfully synthesized from the reduction of Ag+ using AgNO3 solution as a precursor and Brassica rapa var. japonica leaf extract as a reducing and capping agent. This study was aimed at synthesis of AgNPs, exhibiting less toxicity with high antibacterial activity. The characterization of AgNPs was carried out using UV–Vis spectrometry, energy dispersive X-ray spectrometry, fourier transform infrared spectrometry, field emission scanning electron microscopy, X-ray diffraction, atomic absorption spectrometry, and transmission electron microscopy analyses. The analyses data revealed the successful synthesis of nano-crystalline Ag possessing more stability than commercial AgNPs. The cytotoxicity of Brassica AgNPs was compared with commercial AgNPs using in vitro PC12 cell model. Commercial AgNPs reduced cell viability to 23% (control 97%) and increased lactate dehydrogenase activity at a concentration of 3 ppm, whereas, Brassica AgNPs did not show any effects on both of the cytotoxicity parameters up to a concentration level of 10 ppm in PC12 cells. Moreover, Brassica AgNPs exhibited antibacterial activity in terms of zone of inhibition against E. coli (11.1?±?0.5 mm) and Enterobacter sp. (15?±?0.5 mm) which was higher than some previously reported green-synthesised AgNPs. Thus, this finding can be a matter of interest for the production and safe use of green-AgNPs in consumer products.  相似文献   

3.
A simple, eco-friendly phytosynthesis of copper oxide nanoparticles (CuO NPs) using Cassia auriculata leaf extract was reported. The prepared CuO NPs was characterized by UV–vis spectroscopy which exhibited the surface plasmon resonance (SPR) band at 380–385 nm. TEM and EDX analysis confirmed that CuO NPs were spherical and in size range of 30–35 nm with identified elements Cu and O. X-ray diffraction (XRD) spectrum showed the crystalline nature of the prepared CuO NPs. FTIR spectrum confirmed the presence of Cu–O functional groups. CuO NPs showed significant antibacterial efficacy against all the tested bacterial strains, i.e., Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. CuO NPs showed strong antibacterial action against B. subtilis and E. coli than P. aeruginosa and S. aureus. The results of this study revealed that C. auriculata leaf extract was found to be an effective bio-reducing agent for CuO NPs synthesis and also the antibacterial efficacy of phytofabricated CuO may be useful for its applications in medical and textile industries.  相似文献   

4.
The present research is focused on the development of ecofriendly biopolymer blend based nanocomposites to enhance the effect of cytotoxic activity. Novel eco-friendly synthesis of pure Chitosan–Agar blend and Chitosan–Agar/ZnO nanocomposites was successfully synthesized by in-situ chemical synthesis method. The influence of Chitosan–Agar (1:1 wt/wt%) concentrations (0.1, 0.5, 1 and 3 g) was studied. The presence of ZnO nanoparticles in Chitosan–Agar polymer matrix was confirmed by UV, FTIR, XRD, FESEM, EDAX and TEM. The crystallite size of the nanocomposites in the range of 12–17 nm is observed from XRD analysis. PL and UV reveal that Nanocomposites shows an blue shift by increase in the blend concentrations. TEM analysis shows that 0.1 and 3 g of Chitosan–Agar/ZnO Nanocomposites are in spindle and spherical shape with polycrystalline nature. The prepared Nanocomposites shows the respectable Antibacterial activity against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Pseudomonas aureginosa and Klebsilla pneumonia) bacteria. The potential toxicity of Chitosan–Agar/ZnO nanocomposites was studied for normal (L929) and breast cancer cell line (MB231). The result of this investigation shows that the Chitosan–Agar/ZnO nanocomposites deliver a dose dependent toxicity in normal and cancer cell line.  相似文献   

5.
The chitosan Schiff bases were synthesised through the condensation reaction of chitosan with o-, m- and p-nitrobenzaldehyde (abbreviated as CSB-o, CSB-m and CSB-p) in the ratio 1:1 and were characterised by means of FTIR, UV, XRD and SEM. The thermal dehydration and degradation kinetics of all these Schiff bases were studied using different isoconversional and maximum rate (peak) methods, viz. Kissinger–Akahira–Sunose (KAS), Tang, Starink, Flynn–Wall–Ozawa (FWO) and Bosewell from DSC data and the thermal stability from TG. The activation energy values of thermal dehydration and degradation reactions obtained from isoconversional methods of FWO and Bosewell are slightly higher than that obtained from other methods. All the isoconversional and maximum rate (peak) methods yielded consistent values of E α for both the dehydration and degradation reactions and is in the order CSB-o < CSB-m < CSB-p. The Schiff bases observed (from TG) the same order of thermal stability.  相似文献   

6.
Several methods have been developed for synthesis of Ag nanoparticles. However, Ag nanoparticles are unstable materials, and they tend to agglomerate losing their properties. Polymers are commonly employed for the stabilization of Ag nanoparticles in colloidal solutions. Polymers with ionic groups such polyelectrolytes can stabilize metallic nanoparticles through electrostatic and steric effects. In this work we employed poly(p-acryloyloxybenzaldehyde) and their derivates containing arsonic acid groups in ortho and para positions as reducing and stabilizing agents in the synthesis of Ag nanoparticles. Polyelectrolytes containing arsonic acid groups in ortho position were better reducing agents than the poly(p-acryloyloxybenzaldehyde) and the polyelectrolyte with arsonic acid group in para position, leading to the reduction of Ag+ ions in short reaction time. The polyelectrolyte with arsonic acid groups in para position was the best stabilizing agent leading to obtaining Ag nanoparticles with the smallest average size.  相似文献   

7.
Compatibilizer plays very important roles in preparing high performance polymer composites, not only for the ternary immiscible polymer blends, but also for the recycled and reused of waste plastics mixture. Generally, the compatibilizers can be used as the toughening agent in blending polymer materials. In the present work, the poly(styrene-b-(ethylene-co-butylene)-b-styrene) (SEBS) or maleic anhydride-grafted poly(styrene-b-(ethylene-co-butylene)-b-styrene) (SEBS-g-MA) acts as the compatibilizer and toughening agent for the preparation of R-PET/LDPE/SEBS (70/20/10) ternary blends. It must be pointed that the ternary blends are costlessly and conveniently prepared from the recycled poly(ethylene terephthalate) (R-PET) and linear low density polyethylene (LLDPE) through a melt blending in a co-rotating twin-screw extruder and injection moulded. The morphologies of the ternary blends are characterized by scanning electron microscopy (SEM). It was found that the blends contains reactive or non-reactive compatibilizer, the morphology originates from the LLDPE particles encapsulated by both SEBS and SEBS-g-MA. So, it results to the reduced interfacial tension between of the R-PET and SEBS-g-MA, in which the grafted chains of PET-g-SEBS-g-MA formed through in situ reaction between R-PET and SEBS-g-MA phases. Therefore, core–shell particles with smaller diameter disperse uniformly in the blends. Moreover, the good compatibilization and corresponding morphologies induce in balanced mechanical and thermal properties. DSC analysis show the dispersed phase particles could act as nucleating agent in the R-PET matrix, which results the improvement of the crystallization temperature. And it was also observed the decreased nucleation activity in graft copolymers in the R-PET/LLDPE/SEBS-g-MA blends. Notched Charpy impact strength and elongation at break are improved by the addition of compatibilizer.  相似文献   

8.
Atractylodes rhizome is widely used in traditional Chinese herbal medicine. Although the chemical composition of the root has been studied in detail, the oil content and fatty acid composition of the seeds of Atractylodes species have not been reported. Fatty acyl composition of seeds from Atractylodes lancea and A. macrocephala was determined by gas chromatography and mass spectrometry of fatty acid methyl esters and 3-pyridylcarbinol esters. The predominant fatty acid in the seeds of both species was linolenic acid, but the unusual acetylenic fatty acid, crepenynic acid (cis-9-octadecen-12-ynoic acid), was also observed at levels of 18% in A. lancea and 13–15% in A. macrocephala. Fatty acid content was 24% for the samples of A. lancea and 16–17% for samples from A. macrocephala. sn-1,3 regioselective lipase digestion of seed lipids revealed that crepenynic acid was absent from the sn-2 position of the seed triacylglycerol. Crepenynic acid was also found in the seed oil of Jurinea mollis at 24% and was not present in the sn-2 position of the TAG. A contrasting distribution of crepenynic acid was found in the oil of Crepis rubra, suggesting differences in crepenynic acid synthesis or TAG assembly between these species.  相似文献   

9.
Giant silk moths (Lepidoptera: Saturniidae) typically are not well represented as larvae or adults in community level inventories of Lepidoptera, and as a result, little is known about their population dynamics. Furthermore, in recent years, many species of silk moths appear to have experienced population declines. Volatile sex pheromones are powerful sampling tools that can be used in operational conservation and monitoring programs for insects. Here, we describe the identification of the sex attractant pheromone of a giant silk moth, the luna moth Actias luna. Coupled gas chromatography-electroantennographic detection and gas chromatography-mass spectrometric analyses of extracts from pheromone glands of female luna moths supported the identification of (6E,11Z)-6,11-octadecadienal (E6,Z11–18:Ald), (6E)-6-octadecenal (E6–18:Ald), and (11Z)-11-octadecenal (Z11–18:Ald) as the compounds in extracts that elicited responses from antennae of male moths. These identifications were confirmed by synthesis, followed by testing of blends of the synthetic compounds in field trials in Ontario, Canada, and Kentucky, USA. Male moths were attracted to synthetic E6,Z11–18:Ald as a single component. Attraction appeared to be enhanced by addition of E6–18:Ald but not Z11–18:Ald, suggesting that the luna moth pheromone consists of a blend of E6,Z11–18:Ald and E6–18:Ald.  相似文献   

10.
Diaphorina citri is a vector of the bacterial causative agent of Huanglongbing (HLB?=?Citrus greening), a severe disease affecting citrus crops. As there is no known control for HLB, manipulating insect behaviour through deployment of semiochemicals offers a promising opportunity for protecting citrus crops. The behavioural responses of D. citri to plant volatiles, and the identity of these plant volatiles were investigated. Volatiles were collected from host plants Murraya paniculata, Citrus sinensis, C. reshni, C. limettioides, Poncirus trifoliata, and from non-host plants Psidium guajava, Mangifera indica, Anacardium occidentale. In behavioural assays, female D. citri spent more time in the arms containing volatiles from either M. paniculata or C. sinensis compared to the control arms. When D. citri was exposed to volatiles collected from A. occidentale, they preferred the control arm. Volatiles emitted from the other studied plants did not influence the foraging behaviour of D. citri. Chemical analyses of volatile extracts from C. sinensis, M. paniculata, and A. occidentale revealed the presence of the terpenoids (E)-4,8-dimethylnona-1,3,7–triene (DMNT) and (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT) in higher amounts in A. occidentale. In further behavioural bioassays, female D. citri spent less time in arms containing a synthetic blend of DMNT and TMTT compared to the control arms. Female D. citri also spent less time in arms containing the synthetic blend in combination with volatile extracts from either M. paniculata or C. sinensis compared to the control arms. Results suggest that higher release of the two terpenoids by A. occidentale make this species unattractive to D. citri, and that the terpenoids could be used in reducing colonisation of citrus plants and therefore HLB infection.  相似文献   

11.
Self-propagating high-temperature synthesis (SHS) of bulk MgB2 superconductor from Mg-2B powder blend is reported. This reaction proceeds violently at 100 A under a protective atmosphere. Since the heat of reaction of Mg and B was not enough for chain reaction, then (Ti + C) mixed powders were used as the ignition agent to assist the reaction (Mg + 2B). In this case, the combustion front moved without any difficulty. The diffraction lines of the product can be indexed to a hexagonal MgB2 phase, with lattice constants a = 3.0845 Å, and c = 3.5259 Å. For comparison, the direct synthesis of (Mg + 2B) mixture was carried out at (800°C–1000°C). It can be found, that high-temperature sintering (1000°C) will induce the formation of impurities. The MgB2 grains are fine, well compacted and more homogenous. The structure of materials was studied using XRD, FESEM and EDX. M-H curvatures were measured under the magnetic fields between ?80 kOe and 80 kOe. J c was calculated from width of magnetization hysteresis loops based on the extended Bean Model.  相似文献   

12.
A cathode material for lithium-ion batteries–LiNi1/3Co1/3Mn1/3O2–was prepared by solution combustion synthesis and characterized by XRD, SEM, and galvanostatic charge/discharge cycling. The sample calcined at 950°C for 10 h showed best charge/discharge performance. An initial discharge capacity (C) of 150.5 mA h g–1 retained 95.7% of its value after 75 charge/discharge cycles at Ic = 14 mA g–1 (0.2C rate), Id = 70 mA g–1 (0.5C rate).  相似文献   

13.
Poly(N-isopropylmethacrylamide-co-methacrylic acid) [p(NipAam-Mac)] microgels were synthesized and used as microreactors to fabricate silver nanoparticles. Pure and hybrid microgels were characterized using Ultraviolet–Visible (UV/Vis) spectroscopy, Fourier transform infra-red (FTIR) spectroscopy and transmission electron microscopy (TEM). Catalytic activity of hybrid microgels and mechanism of catalysis by this system was explored using different reaction conditions. At the same temperature, apparent rate constant (kapp) was found to be varied from 0.0414 to 0.7852 min?1 by increasing the concentration of NaBH4 from 2.49 to 22.41 mM at constant concentration of substrate and catalyst. However upon extra increase in concentration of NaBH4 from 22.41 to 37.35 mM reduced the value of kapp to 0.2178 min?1. Likewise, the value of kapp was found to be increased from 0.1242 to 0.5495 min?1 with increasing the concentration of 4-nitrophenol [Para-nitrophenol (p-Np)] from 0.063 to 0.079 mM keeping other parameters constant. Further increase in concentration of p-Np caused decline in the value of kapp. Kinetic data reveals that catalytic reduction of p-Np obeys Langmuir–Hinshelwood mechanism and p-Np is converted to p-Ap on the surface of the silver nanoparticles passing through various reaction intermediates.  相似文献   

14.
Derivatives of 2,2-dimethyl-3-(2-methylprop-1-enyl)cyclopropanecarboxylic acid (chrysanthemic acid) are classic natural pyrethroids discovered in pyrethrum plants and show insecticidal activity. Chrysanthemic acid, with two asymmetric carbons, has four possible stereoisomers, and most natural pyrethroids have the (1R,3R)-trans configuration. Interestingly, chrysanthemic acid–related structures are also found in insect sex pheromones; carboxylic esters of (1R,3R)-trans-(2,2-dimethyl-3-(2-methylprop-1-enyl)cyclopropyl)methanol (chrysanthemyl alcohol) have been reported from two mealybug species. In the present study, another ester of chrysanthemyl alcohol was discovered from the striped mealybug, Ferrisia virgata (Cockerell), as its pheromone. By means of gas chromatography–mass spectrometry, nuclear magnetic resonance spectrometry, and high-performance liquid chromatography analyses using a chiral stationary phase column and authentic standards, the pheromone was identified as (1S,3R)-(?)-cis-chrysanthemyl tiglate. The (1S,3R)-enantiomer strongly attracted adult males in a greenhouse trapping bioassay, whereas the other enantiomers showed only weak activity. The cis configuration of the chrysanthemic acid–related structure appears to be relatively scarce in nature, and this is the first example reported from arthropods.  相似文献   

15.
Static and dynamic magnetic properties of SHS-produced BaFe12–2x (Co х Ti х )O19 (х = 1.0, 1.1, 1.2) and SrFe12–2y (Co y Ti y )O19 (0 ≤ y ≤ 1.0) hexaferrites were characterized by magnetization and FMR measurements. Dynamic properties of SrFe12–2y (Co y Ti y )O19 hexaferrites were rationalized in terms of not only magnetic anisotropy but also the anisotropy of magnetomechanical ratio. SHS-produced ferrites can be recommended for designing radar-absorbent coatings and other SHF devices operating in the range 20–50 GHz.  相似文献   

16.
The tail gland of the red fox (Vulpes vulpes) secretes lipids containing volatile terpenes used in social communication. We have analysed lipids extracted from fur of the tail gland, body (flanks) and muzzle of foxes. GC–MS showed a novel group of iso-valerate and tiglate monoesters of alkane-1,2-diols (C18:0–22:0). There was also a larger group of Type II diesters in which a second, longer chain, fatty acid (FA) was attached to the free alcohol group. LC–MS showed the full range of diol diesters, mostly C36:0–50:0, with smaller amounts of the corresponding mono-unsaturated tiglate esters. An additional group of diesters with higher MW (C49:0–62:0) containing two long-chain FA was present in the lipids of body and muzzle fur. After saponification and GC–MS, 98 fatty acids were characterized as their methyl esters. Apart from the C5 FA, most were saturated n-, iso-, anteiso- or other methyl-branched FA (C12:0–28:0) whose structures were determined by a combination of their mass spectra and Kovats retention indices. Several FA have not previously been found in nature or in vertebrates. Thirty-four alkane-1,2-diols were found as their TMS derivatives, mostly n-, iso- or anteiso-isomers of C16:0–25:0. The tail gland had the greatest amount of wax esters, from a greater variety of FA and diols, but lacked the esters with two long-chain FA. These findings show that fox skin lipids comprise mono- and di-esters of alkane-1,2-diols, and exhibit enormous complexity due to the diversity of their constituent FA, diols and the many possible isomers of their esters.  相似文献   

17.
Lead-free BaZr x Ti1–xO3 ferroelectrics (x = 0.2, 0.4, 0.6, 0.8) were synthesized by conventional double-sintering technique. The structural, morphological, and dielectric properties of BaZr x Ti1–xO3 were investigated as a function of zirconium content x. A single-phase perovskite structure of tetragonal ceramics was identified by XRD. The SEM images of the ferroelectrics exhibit fine grains of varied porosity. All synthesized samples exhibited dielectric dispersion at low frequencies.  相似文献   

18.
A novel green fluorescent dye, 4-hexylamino-N-(2-hydroxy-1-hydroxymethylethyl)-1,8-naphthalimide (AHHNA), was designed and synthesized, and then used as a chain extender to prepare a series of intrinsically green fluorescent polyurethane (PU) latexes. The chemical structure of AHHNA and the properties of the fluorescent PU latexes and their films were characterized. It was found that the UV–Vis and the fluorescent spectra of the PUs remained fairly consistent with AHHNA, and the fluorescence emitting capacity of 1,8-naphthalimide group was enhanced by covalently bonding AHHNA onto the PU chain. The amount of AHHNA used in the latex preparation had no obvious effect on the latex colloid properties including particle size, poly index, and zeta potential. Results showed that the color difference (ΔE) and the extraction rate (R e) of the intrinsically fluorescent PU latex film were 5.72% and 3.1%, respectively, while the ΔE and R e of the extrinsically fluorescent PU latex film were 8.31% and 84.7%, respectively, indicating that the light fastness and the solvent fastness of the intrinsically fluorescent PU latex film were much better than those of the extrinsically fluorescent PU latex film.  相似文献   

19.
This mini-review is focused on preparation of polymer protected gold (AuNPs) and silver (AgNPs) nanoparticles that are immobilized on the surface of inorganic supporters and within hydrogel and/or cryogel matrices. A series of water soluble polymers such as poly(N-vinylpyrrolidone), poly(acrylic acid), branched polyethyleneimine, and amphoteric cryogel based on the copolymer of N,N-dimethylaminoethylmethacrylate and methacrylic acid poly(DMAEM-MAA) were used for reduction and stabilization of metal nanoparticles. The catalytic properties of polymer protected AuNPs and AgNPs were evaluated with respect to hydrogen peroxide decomposition, hydrogenation of 4-nitrophenol and oxidation of cyclohexane.  相似文献   

20.
Bracon hebetor is a larval ectoparasitoid that utilizes several pests belonging to the family Pyralidae (Lepidoptera) as hosts. In the present study, we analyzed the kairomonal response of this wasp to the male-produced sex pheromone of a host, the greater wax moth Galleria mellonella, an economically important pest of honeybees, Apis mellifera. Coupled gas chromatography-electroantennographic detection (GC-EAD) revealed three compounds in headspace collections from male G. mellonella that elicited responses from B. hebetor antennae: decanal and the previously identified sex pheromone components, nonanal and undecanal. Y-tube olfactometer tests that used naïve, mated wasps showed that females, but not males, were highly attracted to (a) male G. mellonella headspace samples, (b) two synthetic blends of nonanal and undecanal (in ratios matching that found in male moth samples), and (c) the two aldehydes tested individually. Further, female wasps did not discriminate between a blend of aldehydes and male G. mellonella headspace. In dose-response trials that used octanal, nonanal, decanal, and undecanal, no difference in EAG responses of the two sexes was observed, except for undecanal at the second highest dose, for which female antennae showed significantly larger responses than did male antennae. When the two binary blends were tested at different doses, female wasps were significantly attracted to the two highest doses (1 µg and 10 µg), but not to the lowest dose (100 ng). Our results show that females of this economically important parasitoid utilize the male-produced sex pheromone of a host as an indirect cue to guide them to potential oviposition sites.  相似文献   

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