免疫亲和净化-光化学衍生-高效液相色谱法检测动物肝脏中六种黄曲霉毒素

目的以鸡、猪等动物肝脏为研究对象,二氯甲烷为提取溶剂,建立免疫亲和柱净化、高效液相色谱-光化学衍生-荧光检测器检测六种黄曲霉毒素的分析方法。方法样品经提取、过滤、浓缩、复溶后经免疫亲和柱净化,进样后采用光化学衍生器在线对黄曲霉毒素G1、B1衍生,荧光检测器分析B1、B2、G1、G2、M1、M2六种黄曲霉毒素。结果在不同加标水平,不同肝脏回收率在70.2%~94.1%之间,变异系数为2.67%~12.90%,检测低限为0.16μg/kg。结论该方法的准确度与精密度较高,可以满足痕量分析要求。     

免疫亲和柱净化-光化学柱后衍生高效液相色谱 荧光法测定粮食中黄曲霉毒素

建立了粮食中黄曲霉毒素B1、B2、G1、G2的免疫亲和柱净化-光化学柱后衍生高效液相色谱荧光检测法。样品经甲醇-水提取,免疫亲和柱净化,高效液相色谱分离,光化学柱后衍生,荧光检测器测定。结果表明,黄曲霉毒素B1、B2、G1、G2的检出限分别为0.50、0.25、0.50、0.25μg/kg,回收率为67.2%～91.7%,RSD小于10%。该方法快速、准确、灵敏度高、重现性好,能满足我国对粮食中黄曲霉毒素限量的检测要求。     

免疫亲和柱净化-在线柱后光化学衍生-高效液相色谱-荧光检测法快速测定食品中的黄曲霉毒素含量

目的 通过建立免疫亲和柱净化-在线柱后光化学衍生-高效液相色谱-荧光检测法探索快速测定食品中的黄曲霉毒素含量的方法。方法 样品经甲醇-水(V/V, 7︰3)提取, 免疫亲和柱净化、富集、淋洗后, 收集洗脱液, 洗脱液经Acclaim?120 C18 柱分离, 在254 nm紫外光下进行光化学衍生, 用荧光检测器进行测定。结果 黄曲霉毒素G2, G1, B2, B1检测限均小于2 μg/kg, 加标回收率为94.3%~108.8%。 结论 该方法能连续操作, 节省人力, 可用于食品中黄曲霉毒素含量的测定。     

IAC-HPLC法检测牛奶中黄曲霉毒素和玉米赤霉醇及类似物质量分数

建立了IAC-HPLC(免疫亲和柱净化-高效液相色谱)法检测牛奶中6种黄曲霉毒素和玉米赤霉醇及类似物的方法。样品经免疫亲和柱净化后,黄曲霉毒素用高效液相色谱——荧光检测器柱后衍生检测,玉米赤霉醇及其类似物用高效液相色谱——紫外检测器检测。结果表明,牛奶中黄曲霉毒素(M2,M1,G2,G1,B2,B1)的检测限分别为0.004,0.004,0.004,0.003,0.002,0.002μg/L,6种黄曲霉毒素的平均回收率在91.20%~113.8%之间,变异系数小于8.79%;玉米赤霉醇及其类似物的检测限均为0.05μg/L,平均回收率在54.22%~90.76%之间,变异系数小于9.44%。     

坚果中黄曲霉毒素的光化学柱后衍生-高效液相色谱法测定

目的建立坚果中黄曲霉毒素B1、B2、G1、G2的高效液相色谱荧光检测器测定方法。方法样品以甲醇-水(70:30,v/v)溶液匀质提取,过黄曲霉毒素总量免疫层析亲和柱净化,经LaChrom C18色谱柱分离和光化学柱后衍生反应器衍生后,用带有荧光检测器的高效液相色谱仪测定。采用峰面积外标法定量坚果中黄曲霉毒素B1、B2、G1、G2含量。结果四种黄曲霉毒素在各自的浓度范围内线性关系良好,相关系数均大于0.999,B1、B2、G1、G2的检出限依次为0.10、0.05、0.10、0.05μg/kg。在3个添加水平下回收率为77.5%~109.8%,相对标准偏差为1.43%~2.71%。结论该方法的灵敏度、准确度、精密度均符合黄曲霉毒素的检测技术要求,适用于坚果中黄曲霉毒素B1、B2、G1、G2的日常检测。     

免疫亲和层析净化-高效液相色谱法同时测定牛奶和奶粉中黄曲霉毒素B1、B2、G1、G2、M1、M2

建立牛奶和奶粉中黄曲霉毒素B1、B2、G1、G2、M1、M2的免疫亲和层析净化和柱后衍生高效液相色谱测定方法。样品经溶解、离心、过滤后,通过免疫亲和柱,黄曲霉毒素特异性抗体选择性地与存在的黄曲霉毒素抗原键合,形成抗体-抗原复合体。甲醇-乙腈混合溶液(4:5,v:v)洗脱,带荧光检测器的高效液相色谱仪经柱后衍生测定,外标法定量。标准曲线线性良好,添加回收率在57.0%～88.7%,相对标准偏差在3.37%～16.9%,牛奶中各黄曲霉毒素检出限:B1为2ng/kg,B2为1ng/kg,G1、G2为3ng/kg,M1、M2为5ng/kg;奶粉中B1为20ng/kg,B2为10ng/kg,G1、G2为30ng/kg,M1、M2为50ng/kg,检测低限能够满足各国对牛奶和奶粉中黄曲霉毒素的限量要求。该方法准确、快速、灵敏度高,适用于牛奶和奶粉中黄曲霉毒素的测定。     

HPLC-柱后光化学衍生法检测花生酱中黄曲霉毒素

建立高效液相色谱-在线柱后光化学衍生-荧光检测器检测花生酱中黄曲霉毒素B1、B2、G1、G2的含量。样品以乙腈-水(80∶20)溶液提取,经免疫亲和柱净化后,利用在线柱后光化学衍生-HPLC-FLD进行分析测定。结果:在优化条件下,黄曲霉毒素B1、G1在0.30 mg/L~10 mg/L,黄曲霉毒素B2、G2在0.06 mg/L~3.0mg/L线性关系良好,r0.998,回收率80%~101%,RSD5.9%。黄曲霉毒素B1、B2、G1、G2的检测限(LOD)分别为0.10、0.03、0.15、0.04μg/kg。     

免疫亲和柱净化-柱后光化学衍生-高效液相色谱法检测粮油中黄曲霉毒素

介绍了同时采用免疫亲和柱净化-柱后光化学衍生-高效液相色谱方法检测粮油中黄曲霉毒素B1、B2、G1和G2。样品经过甲醇-水(体积比为70∶30)提取,通过免疫亲和柱富集和净化,采用Cloversil C18色谱柱(4.6 mm i.d.×150 mm,粒度5μm),以甲醇∶水(50∶50)溶液为流动相,柱后光化学衍生、利用荧光检测器检测,黄曲霉毒素B1、B2、G1和G2检出限分别为0.5,0.2,0.7和0.2μg/kg,标准曲线的线性范围分别为:0.51～10.19 ng/mL,0.15～2.92 ng/mL,0.54～10.71 ng/mL,0.16～3.3 ng/mL,在稻谷、食用油样品中,平均加标回收率为70.3%～109.8%,相对标准偏差为1.4%～9.1%,说明回收率、精密度均可以满足实际工作的要求。     

高效液相色谱-柱后衍生法测定植物油脂中 4种黄曲霉毒素

目的建立一种可同时测定植物油脂中黄曲霉毒素G2、G1、B2、B1残留量的分析方法。方法将待测样品经前期溶剂提取、免疫亲和柱净化处理后,通过高效液相色谱-柱后衍生-荧光检测器进行测定。结果黄曲霉素G2、B2在0.1~2 ng/m L范围内表现出良好的线性关系,而黄曲霉素G1、B1在1~20 ng/m L范围内r0.9995,其线性关系良好。在添加浓度为0.1~20μg/kg的范围内,平均回收率在75.0%~125.7%之间。结论该方法快速简便准确,可作为植物油脂中黄曲霉毒素的残留量测定方法。     

免疫亲和柱净化-大体积流通池高效液相色谱法同时检测食品中6种黄曲霉毒素

建立了免疫亲和柱净化-大体积流通池无需衍生同时测定食品中6种黄曲霉毒素(Aflatoxins)的检测方法。样品采用乙腈-水(84:16,V/V)超声辅助提取,用免疫亲和柱净化,经过XBridge TM C18柱(150 mm×4.6 mm,5μm)分离。采用乙腈-甲醇-水(15:25:60,V/V)为流动相,大体积流通池荧光检测器检测,无需衍生,外标法定量。6种黄曲霉毒素在9.5 min内有效分离,从样品前处理到结果分析整个过程小于50 min。黄曲霉毒素B1、B2、G1、G2、M1、M2的检出限(LOD,S/N=3)分别为0.05μg/kg、0.02μg/kg、0.05μg/kg、0.02μg/kg、0.04μg/kg、0.03μg/kg,能够满足我国对食品中黄曲霉毒素限量的要求。6种黄曲霉毒素标准曲线线性良好,线性相关系数r2大于0.999;样品加标回收率为77.6%~90.5%,精密度RSD为2.42%~6.08%。本方法简便、准确、灵敏度高,无需衍生即可同时检测食品中6种黄曲霉毒素,适用于食品中6种黄曲霉毒素的快速定量测定。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press     

INFOPRINT 2014 was Successfully Held in Dongguan

Sponsored by Printing and Printing Equipment Industries Association of China （PEIAC） and organized by Print China magazine, the Seventeenth Beijing International Printing Information Conference （INFOPRINT 2014） was successfully held on 11th Dec. 2014 at Dongguan Exhibition International Hotel.