Studies on Desi and Kabull Chickpea (Cicer arietinum L.) Cultivars: Levels of Protease Inhibitors, Levels of Polyphenolic Compounds and in vitro Protein Digestibility

The levels of trypsin inhibitor activity were higher in both kabuli and desi seeds of chickpea than their chymotrypsin inhibitor activity. Mean values for the trypsin and chymotrypsin inhibitor units in dhal and seed samples of desi were higher as compared with kabuli cultivars. The presence of seed coat reduced the protein extraction. Mean values of polyphenolic compounds in seed samples of desi were more than twice that of kabuli and these differences disappeared in dhal samples indicating the distribution of these compounds mainly in the seed coat. The in vitro protein digestibility studies showed larger differences between desi seed and dhal samples when compared with kabuli seed and dhal samples. Polyphenolic compounds exhibited a highly significant and negative corretation (r = 0.872**) with in vitro digestibility of protein and a significant positive correlation with trypsin (r = 0.612*) and chymotrypsin (r = 0.507*) inhibitor activities.     

Variability of Some Physico-chemical Characters in Desi and Kabuli Chickpea Types

A collection of 50 chickpea accessions (26 kabuli and 24 desi types) was evaluated for 2 years for eight physico-chemical seed characters: 100-seed weight, hydration capacity, hydration index, coat thickness and contents of protein, oil, acid detergent fibre (ADF) and starch. Significant differences were found between desi and kabuli types for the majority of the characters. The variance component due to the genotype×year interaction was important for the hydration index, starch and protein content, showing the importance of the year effect on genotypic expression of these characters. One kabuli accession and five desi accessions with high and stable protein content were selected. There was no overlap between the variation limits of desi and kabuli for coat thickness and ADF content. There were high positive and significant correlations between seed weight and oil content for both types of chickpea.     

Chemical analysis and physico-chemical properties of chickpea and lentil cultivars

Some high yielding cultivars of chickpea (Kabuli and desi) (Cicer arietinum) and lentil (Lens esculenta) were used for chemical analysis and physico-chemical properties namely protein, fat, sugars, starch, in vitro digestibility of starch and protein; seed weight, seed volume, seed density, hydration capacity, swelling capacity, water absorption capacity and cooking time. Among chickpea, Gora Hisari (Kabuli) and Haryana Chana (desi) cultivars, and among lentil LH 82-6 cultivar manifested higher contents of protein, fat, sugars, starch and in vitro digestibility of starch and protein. Values of seed volume, seed density, hydration capacity, swelling capacity and water absorption capacity were also found higher for these cultivars which might have contributed towards less cooking time.     

Studies on Desi and Kabuli Chickpea (Cicer arietinum L.) Cultivars. The Levels of Amylase Inhibitors, Levels of Oligosaccharides and In Vitro Starch Digestibility

Amylase inhibitor activity (AIA) of chickpea extracts was investigated using pancreatic and salivary amylases. The extracts showed higher inhibitor activity towards pancreatic amylase than salivary amylase. Mean values indicated slightly higher inhibitory activity in desi than kabuli cultivars, though clear-cut differences were not observed among the cultivars. While in vitro starch digestibility of meal samples indicated no large differences among desi and kabuli types of chickpea, the mean values of digestibility of isolated starches of kabuli types was higher than those of desi types. The mean values of stachyose were higher in desi cultivars. When desi and kabuli types were considered together, stachyose and raffinose contents were not found significantly related to the concentrations of total soluble sugars while stachyose showed a significant correlation with raffinose.     

Amino Acid and Elemental Contents of Lima Bean Seed

A study was conducted with 17 cultivars of lima beans whose seed coat color was white, green or speckled and whose cotyledon color was white, green and mixed. Kjeldahl nitrogen, 17 amino acids and 8 elements were determined in the seed. The lima bean was found to be a good source of most amino acids except for the essential amino acid methionine, which was the limiting amino acid. Seven elements were supplied at an RDA level of 10% or better. No significant differences in the amino acids or the 8 elements were found among cotyledon or seed coat color. However, differences were found among cultivars.     

Relationships Between Selected Properties of Seeds,Flours, and Starches from Different Chickpea Cultivars

ABSTRACT

Five desi (PBG-1, PDG-4, PDG-3, GL-769, and GPF-2) and one kabuli type (L-550) chickpea cultivars were evaluated for their seed mass, volume, hydration capacity, swelling capacity, cooking time, and instrumental textural properties (hardness, cohesiveness, gumminess, and chewiness). Flour was prepared from these chickpea cultivars and various physicochemical and functional properties were determined. The pasting (pasting temperature, peak viscosity, breakdown, and final viscosity) and gelatinization (T _o, T _p, T _c, and ΔH _gel) properties of these flours were measured using Rapid Visco Analyzer (RVA) and Differential Scanning Calorimeter (DSC), respectively. Starch was also isolated from chickpea cultivars and evaluated for amylose content, swelling power, solubility, and syneresis values. Physicochemical, cooking, and instrumental textural properties of seeds of different chickpea cultivars were related to physicochemical, gelatinization, and pasting properties of their flours and physicochemical properties of their starches. Selected properties of chickpea seeds were significantly correlated with the properties of their starches and flours. Hardness value of soaked chickpea seeds was positively correlated to cooking time, seed mass, seed volume, hydration, and swelling capacity (p < 0.01). Water solubility index (WSI) of chickpea flours was positively correlated to seed mass, volume, hydration capacity, and hardness value (p < 0.05). Selected instrumental textural parameters of seeds had positive correlation with ΔH _gel of flours (p < 0.01). Peak viscosity of flours showed positive correlation to breakdown, final viscosity, bulk density, and negative correlation to cohesiveness of soaked seeds (p < 0.01). Final viscosity showed negative correlation to bulk density and water absorption index (WAI) (p < 0.01) of flours.     

A Method to Estimate the Hydration and Swelling Properties of Chickpeas (Cicer arietinum L.)

ABSTRACT:  Existing methodologies for estimating hydration properties in pulses (a potential indicator of cooking time) can be misleading and few measure both maximum hydration and the rate of hydration. Therefore, a new method, based on the Mitscherlich equation, was proposed and compared to existing methodologies. It was shown to be a superior estimator of both maximum hydration and hydration rate in desi and kabuli chickpeas. The new method was also superior for estimating maximum swelling and the rate of swelling in desi and kabuli chickpeas. Hydration and swelling data for 6 desi and 4 kabuli chickpea genotypes were compared using the new method and showed large genotypic differences. Use of the new method is recommended for estimating the hydration parameters of desi and kabuli chickpea for industry purposes and for selection of genotypes in breeding programs.     

Physicochemical Characteristics of 18 Cultivars of Nigerian Cowpeas (V. uniguiculafa) and Their Cooking Properties

Eighteen cultivars of cowpeas (V. unguiculata) were analyzed for cooking time, swelling capacity, leached solid, percent seed coat, seed density, amylose, tannins, proteins, and soluble sugars. The physicochemical characteristics varied with the seed coat color, the hilum color, and the skin texture. The cooking time was positively correlated to protein, seed density, percent seed coat, swelling capacity and leached solids, and negatively correlated to the levels of amylose, soluble sugars and tannins. There were significant correlations (p < 0.05) between sugars and swelling capacity, protein and seed density; and seed density and swelling capacity. The mean percent seed coat was significantly different (p < 0.05) between the varieties with wrinkled seed coats and those with smooth seed coats.     

Subunit,amino acid composition and in vitro digestibility of protein isolates from Chinese kabuli and desi chickpea (Cicer arietinum L.) cultivars

The subunit, amino acid composition and in vitro digestibility of the two protein isolates (GCPI and ZCPI) from one kabuli and one desi chickpea cultivars, grown extensively in Xinjiang Autonomous Region of China, were investigated and compared with those of soy protein isolate (SPI). SDS–PAGE showed that GCPI and ZCPI had almost the same band components under the reduced and unreduced conditions, with only minor difference in relative quantity for some bands, but different from that of SPI. The sulphur-containing amino acids were the first limiting amino acids for all three protein isolates of GCPI (2.11 g/100 g), ZCPI (2.20 g/100 g) and SPI (1.99 g/100 g). Amino acid score of the three protein isolates could reach the FAO/WHO requirement (1990) for the essential amino acids for preschool children. The order of in vitro digestibility was GCPI (87.47%) > ZCPI (80.82%) > SPI (71.04%). Our results indicated that, compared with soybean protein isolate, Chinese kabuli and desi chickpea protein isolates had higher digestibility value, and chickpea protein, especially for kabuli protein, could be utilized as a good source of protein for human nutrition.     

Chemical basis for the low sensory perception of the Ganxet bean (Phaseolus vulgaris) seed coat

The Ganxet bean (Phaseolus vulgaris L), highly appreciated in Catalan gastronomy, has a higher proportion of seed coat than other beans (eg White Kidney or Faba Asturiana); however, general diners and sensory tests point out a lower perception of this seed coat. The differences in chemical composition between Ganxet, White Kidney and Faba Asturiana beans, before and after cooking, were established and related to perceived sensory differences in seed coat content. Ganxet's higher absolute and percentage losses of total dietary fibre (19.5%), soluble dietary fibre (32.5%), cellulose (25.6%) and pectins (38.1%) break down the consistency of the seed coat during cooking, which would explain its low sensory perception. The high loss of cellulose during cooking could be due to its partial combination with proteins, which would aid its breakdown. © 2002 Society of Chemical Industry     

Genotype and growing environment influence chickpea (Cicer arietinum L.) seed composition

BACKGROUND: As a first step towards genetic improvement of seed quality in chickpea (Cicer arietinum L.), seven desi and nine kabuli varieties were grown at multiple sites to assess the affect of environment on seed yield, weight and selected seed constituents. The sites were chosen to represent a range of environments in chickpea production areas of the Canadian prairies. RESULTS: Genotype × environment interaction effects on starch, amylose and protein (desi only) concentrations and seed yield were significant, suggesting that the varieties did not perform consistently relative to each other in the different environments. Starch concentration was negatively correlated (r_kabuli = ?0.25, P < 0.05; r_desi = ?0.16, P < 0.05) with protein concentration in both chickpea market classes. However, repeatability estimates of starch, amylose and protein concentrations were low and inconsistent across chickpea market classes, possibly owing to complex biosynthetic pathways for these constituents. CONCLUSION: The results suggest that testing for seed constituent traits over a range of environments will be required to improve seed quality in individual chickpea varieties. The best selection strategies for seed constituent improvement in chickpea will be influenced by genotype and genotype × environment interaction for these traits. The negative relationship between seed constituents and yield indicates that selection for chickpea cultivars with desired seed composition may require compromise and indirect selection. Copyright © 2009 Crown in the right of Canada. Published by John Wiley & Sons, Ltd.     

Comparison of sprout quality characteristics of desi and kabuli type chickpea cultivars (Cicer arietinum L.)

Effect of germination time on comparative sprout quality characteristics (proximate composition, ascorbic acid, phytic acid, invitro protein digestibility, protein solubility and sensory properties) was investigated. Sprouting significantly increased (P<0.01) the moisture, crude protein, crude fat and ascorbic acid contents and decreased nitrogen free extract (NFE). Phytic acid was reduced (P<0.01) with sprouting, but more pronouncedly so in the case of Kabuli type (73% reduction) than in desi type (32% reduction). In-vitro protein digestibility (IVDP) and protein solubility improved significantly (P<0.01) with increase in sprouting time. The overall sensory scores increased with the first (24 h) sprouting interval and then decreased. However, the acceptability scores for both chickpea types remained within the acceptable range (>5.0). Although, organoleptically the desi type chickpea sprouts were preferred over Kabuli type, the nutritional improvement due to sprouting was more pronounced in Kabuli chickpeas than their desi counterparts. Due to overall superior sprout quality, the kabuli type was more suitable for sprouting purposes.     

Chemical composition and protein quality of newly released groundnut (Arachis hypogaea L ) cultivars

Five groundnut (Aruchis hypogueu L) cultivars developed by the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) and two local cultivars as controls were grown in post-rainy and rainy seasons at Patancheru, India. They were analysed for their proximate composition, minerals and trace elements, amino acid composition, true protein digestibility (TD), biological value (BV), net protein utilisation (NPU), and protein efficiency ratio. One-hundred seed mass, protein content and TD were significantly higher in the post-rainy season while starch, sugars, BV and NPU were significantly higher in the rainy season. Post-rainy cultivars exhibited higher concentrations of potassium, calcium, iron and several essential and non-essential amino acids. TD of blanched groundnut was better than that of the whole seeds. The composition and protein quality of ICRISAT cultivars were comparable to those of the controls.     

Variation among physical,chemical and technological properties in three Sicilian cultivars of Chickpea (Cicer arietinum L.)

Three Sicilian chickpea cultivars (‘Calia’, ‘Etna’ and ‘Principe’) were evaluated for physical, chemical and technological properties. Whereas no substantial differences were ascertained on seeds chemical composition, the cultivars greatly differed in terms of seed size, specific surface area (SSA) and seed coat incidence. These last two properties affected the technological properties of the seeds. In particular, a high SSA (‘Calia’) contributed to a faster softening, whilst a great coat thickness (‘Etna’) delayed both hydration and softening rate. The time required to obtain an acceptable firmness (cooking time) was greatly reduced by presoaking the seeds in salt solution (0.5% NaCl or NHCO₃). ‘Calia’ required the lowest cooking time when soaked in distilled water or in 0.5% NaCl. Presoaking seed in NaHCO₃ allowed halving the cooking time in all cultivars.     

Nutritive value of mango seed kernel

Seed kernels of two cultivars (Chausa and Dusheri) of mango (Mangifera indica) were analysed for chemical composition, lipid classes, fatty acid composition, amino acid profile and chemical evaluation of protein quality. The seed kernels constituted about 18% of the total fruit and had 5% protein, 6–7% crude fat, 0.19–0.44% tannins, iodine value of 34–44 and saponification number 202–213. Oleic acid (42%) and stearic acid (39%) were the principal fatty acids in the oil. The in vitro digestibility was low in these cultivars, possibly due to the presence of tannins. Sulphur-containing amino acids (methionine+cystine) and isoleucine were the limiting amino acids in Chausa and Dusheri, respectively. The essential amino acid index and protein quality index were high, thus indicating the good quality of the protein in mango seed kernel.     

模拟传统柴火烹饪方式对米饭食味及蛋白质营养特性的影响

研究不同烹制方式对米饭食味品质、蛋白质营养特性的影响。结果表明,烹制方式对米饭食味品质和蛋白质营养特性具有较大影响,快速米饭和柴火米饭比传统柴火米饭滋味好,硬度大、黏弹性好,挥发性风味物质种类多。传统柴火米饭醛、醇挥发性物质总量、必需氨基酸指数较高。电饭煲烹制方式对米饭香气、滋味、挥发性风味物质、还原糖、质地、氨基酸组成均具有较大影响,柴火米饭香气、滋味较好,醛、酮、醇、酚挥发性物质总量较高,且质地柔软黏弹、必需氨基酸指数最高。主成分分析提取了前4个主成分,累计方差贡献率达到88.22%,表明不同烹制方式米饭食味与营养品质可以分为非必需氨基酸组成、挥发性风味物质与必需氨基酸组成、外观特性和黏弹性、感官品质4类,主成分综合评分得分顺序由高到低分别是传统柴火米饭、柴火米饭、快速米饭。     

不同品种杏仁氨基酸组成分析及综合评价

选取新疆种植的10 种杏仁作为研究对象,通过必需氨基酸指数（essential amino acid index,EAAI）和氨基酸比值系数分（score of ratio coefficient of amino acid,SRC）等化学指标对杏仁蛋白质营养价值进行系统分析;并在主成分分析和聚类分析的基础上对10?种杏仁的品质进行综合评价。结果表明杏仁总氨基酸质量分数在16.7%～23.12%之间,必需氨基酸与总氨基酸比值在32.18%～33.75%之间,EAAI值＞100,SRC值＜100,且品种间差异较大,其中限制性氨基酸为含硫氨基酸和赖氨酸。通过主成分分析提取3?个主成分,累计方差贡献率为90.295%,由每个主成分贡献率重新建立的综合评价模型较好地反映了杏仁蛋白质的综合信息,并将10?种杏仁按品质排名。聚类分析将10?种杏仁分为4?类,结果与主成分分析一致。     

Characterization of protein isolates from different Indian chickpea (Cicer arietinum L.) cultivars

Protein isolates prepared by alkaline solubilization followed by isoelectric precipitation and freeze drying from desi (PBG-1, PDG-4, PDG-3, GL-769, and GPF-2) and kabuli (L-550) chickpea cultivars were evaluated for functional (water and oil absorption capacities, least gelation concentration, foaming capacity and stability) and thermal properties. Significant difference (P ? 0.05) in properties of kabuli and desi chickpea protein isolates was observed. Kabuli chickpea protein isolate showed significantly (P ? 0.05) higher ash (1.14%), protein (94.4%), L^∗, ΔE value, oil absorption capacity (OAC) and lower water absorption capacity (WAC) than their corresponding desi chickpea protein isolates. The solubility-pH profile of different protein isolates showed minimum solubility in the pH between 4.0 and 5.0 and two regions of maximum solubility at pH 2.5 and 7.0. Foaming capacity of all protein isolates increased with the increase in concentration. Kabuli chickpea protein isolate showed the highest foam stability (94.7%) after 120 min of storage. The thermal properties of protein isolates from different chickpea cultivars were studied by differential scanning calorimetry (DSC). Protein isolates from both the chickpea types differed significantly (P ? 0.05) in peak denaturation temperature (T_d) and heat of transition (ΔH). Kabuli type protein isolate exhibited lower T_d and ΔH value as compared to those from desi types. The interrelationships between characteristics of protein isolates showed a significant (P ? 0.05) negative correlation of T_d with protein content and OAC. It was also observed that cultivars with high fat content had high ΔH and lower WAC.     

SEED COAT EFFECTS IN COOKED RECONSTITUTED BEAN TEXTURE

The contribution of seed coat to cooked reconstituted bean texture was assessed by measuring sensory and instrumental parameters of beans with and without coats and also seed coats alone. Experiments using white beans stored for one year under tropical conditions to induce the hard-to-cook (HTC) defect or temperate conditions both resulted in reductions in the order of 50% in puncture force and sensory properties of beans when the seed coat had been removed. HTC bean texture was influenced less by absence of the seed coat than texture of soft beans. Seed coats softened during cooking but those from HTC beans softened less than those from soft beans. It is possible that seed coats harden during tropical storage by a lignification-type mechanism. Beans with harder seed coats absorbed less water during soaking which may contribute further to bean hardness.     

20种中国蚕豆的烹煮加工适用性分析

为探索我国蚕豆烹煮加工的适用性,在前期选取了来自全国各主产区的20种蚕豆作为分析对象,对其化学组分、物理特性进行了测定和分析,在此基础上,继续对其烹煮品质进行评价,结果发现通鲜l号等5个品种的烹煮品质最佳.将蚕豆的理化性质与烹煮品质三者之间进行相关性分析,结果表明,蚕豆的灰分含量与蚕豆的物理性质、烹煮品质相关性最高,说明蚕豆的烹煮加工品质特性受地域土壤环境影响很大.影响烹煮品质的其他指标依次是子叶淀粉、皮重比例、皮粗纤维、子叶直链淀粉、子叶蛋白,而物理指标中的百粒体积可作为预测蚕豆烹煮品质的重要指标.